Objective To investigate expression and significance of matrix metalloproteinase-9 (MMP-9) and neutrophil gelatinase-associated lipocalin (NGAL) in serum and placental tissue of preeclampsia maternal.Methods 74 cases of preeclampsia pregnant women as research subjects,which 39 cases of severe preeclampsia,35 cases of mild preeclampsia,selected 30 cases of normal late maternal as the control group.Three groups of maternal serum levels of MMP-9 and of NGAL were detected by enzyme-linked immunosorbent assay.RT-PCR was used to test placenta MMP-9 mRNA,and NGAL mRNA level.Results Preeclampsia maternal serum levels of MMP-9 and NGAL level were significantly higher than those of severe preeclampsia pregnant women serum level of MMP-9 and NGAL were significantly higher than mild preeclampsia (t =3.127,2.846,3.508,3.284,all P ＜ 0.05).Preeclampsia placentas of MMP-9 mRNA and NGAL mRNA levels were significantly lower than the control group (t =-2.842,-2.728,-3.532,-3.276,(a)ll P ＜ 0.05),severe preeclampsia placentas of MMP-9 and NGAL were significantly higher than mild preeclampsia(t =2.732,2.741,all P ＜ 0.05),severe preeclampsia placentas of MMP-9 mRNA and NGAL mRNA levels were significantly lower than in patients with mild preeclampsia (t =-2.681,-2.587,all P ＜ 0.05).Preeclampsia maternal serum levels of MMP-9 and NGAL was positively correlated with placental tissue mRNA levels of MMP-9 and NGAL mRNA level was positively correlated (P ＜ 0.05).Conclusion Preeclampsia maternal serum MMP-9 and NGAL were significantly elevated,placental MMP-9 mRNA and NGAL mRNA levels were significantly decreased,suggested that they may be involved in the pathogenesis of preeclampsia.

Rapid and accurate diagnosis of pathogens is the responsibility of the clinical microbiology lab.Effective implementation of this function requires standard procedure of specimen collection and processing.In order to improve the ability of infectious disease diagnosis,clinical microbiology laboratory should strengthen the communication with the clinicians,to improve the quality and quantity of specimens.Meanwhile,microbiology laboratory should learn some clinical knowledge and understand the demand and development of clinical practice.Clinical microbiology lab should emphasize patient-oriented and multidisciplinary close cooperation,for improvement of pathogen diagnosis level.

Objective To observe the repairing effect of hyaluronic acid hydrogel on rat articular cartilage defects .Methods One hundred and twenty SD rats were randomly divided into group A (natural repair group) ,group B (hyaluronic acid group) and group C (composite material group) ,36 rats was in each group and 12 rats were used to obtain cartilage cells .Full‐thickness articular os‐teochondral defects(2 .5 mm in diameter and 3 .0 mm in depth)were created mechanically in femoral trochlea of rats .Histological and biochemical method were used to evaluate the repairing effect of different material at different points(10 ,20 ,40 d) .Results Repairing effect of group C was best by histological and biochemical method at different points ,followed by group B ,and they were better than that of group A .Conclusion Hyaluronic acid hydrogel could provide cartilage cell proliferation for porous space truss structure ,and could carry cartilage cells at the same time .the cellular space truss structure have high surrounding tissue compatibil‐ity and biodegradation ,it is helpful for cartilage cell survival ,reproduction and division .

The soluble antigen was isolated from pig retinal extract by precipitation with ammonium sulfate followed by molecular sieve. 50 ?g S antigen with equal volume complete Freund's adjuvant was injected into double hind foodpads of guinea pigs. Two weeks later, experimental autoimmune uveitis developed. Its pathologic feature is that a great number of lymphocytes infiltrated into chroid which was thickened gradually from anterior to posterior. Inflammatory variation was relative to disease phase. Ultrastructure pathology was characterized by swelling and necrosis in the inner and outer segments of photorecepter cells. This result suggests that S antigen has significant effect on inducing experimental autoimmune uveitis and the outer layer of retina is the sensitive target organ of S antigen.

AIM Protective action of TMP on hearts and brains under the condition of the acute hypoxia was studied. METHODS On the device of mice acute hypoxia ,ability of tolerancing hypoxia of mice was investigated,and superoxide dismutase(SOD) and malondialdehyde(MDA) and nitric oxide(NO) contents in hearts and brains were measured.RESULTS TMP could make the resisting hypoxia action in mice strengthen significantly,and the action had a does dependent increase. TMP made SOD contents in hearts and brains in does dependent increase;MDA contents in hearts and brains were not notable in low does ,but TMP could decreased MDA contents in hearts and brains in middle and high does ( P

In this paper, some study methods of pharmacokinetic, the explanation ways of data and experimental methods of transdermal therapeutic system(TTS) were described. The advancement of modern science accelerates the development of pharmacokinetic of TTS and makes more progress in this field.

Objective:To discuss the diagnosis,treatment of uterus cornea pregnancy.Methods:Retrospective analysis was made in 21 cases of uterus cornua pregnancy is recent 10 years.Results:8 cases were diagnosed uterus cornua,9 cases were misdiagnosed ectopic pregnancy,1 case was misdiagnosed rupture of uterus of artifical obortion,1 case was misdiagnosed pregnancy with uterus septus,1 case was misdiagnosed choriocarcinoma.Surgical treatment was done in 19 patients,and the other 2 cases received conservative therapy.Conclusion:Cornual pregnancy is often misdiagnosed as ectopic pregnancy or other diseases.More careful gynecological and transobdominal ultrasound examination will improve the accuracy of diagnosis.

Objective To study the clinical value of procalcitonin on early diagnosis of sepsis .Methods 60 patients who entered ICU were enrolled in the present study were chosen .The change of procalcitonin , CRP, WBC,ESR,GRANR and the highest temperature was observed dynamically with 1 day and 4 days in hospitalization . The patients were ultimately divided into the two groups according to the diagnostic standard of sepsis ,the sepsis group (SIRS group) and infective systemic inflammatory response syndrome group (NONSIRS group).Results After ana-lyzing the processed data,researchers found that PCT of the sepsis group (6.73 ±1.31)ng/mL was higher evidently than that of the SIRS group(0.25 ±0.05)ng/mL(t=3.10,P<0.05),the area under ROC curve of PCT (0.869) is evidently greater than CRP(0.798),WBC(0.737) and ESR(0.394)(P<0.05),which had statistical significance. Conclusion Procalcitonin is a good index of early diagnosis on sepsis ,therefor,its sensitivity and specificity is higher than the other inflammatory indicators .Finally,procalcitonin can be widely used in clinical trial .

Objective To observe the clinical efficacy of Xing Nao An Shen Jie Yu needling method (brain-awakening mind-calming and anti-depression needling method) in treating depression after cerebral stroke. Method Totally 120 patients with depression after cerebral stroke were randomized into two groups: the acupuncture group was intervened by An Shen Xing Nao Jie Yu needling method, while the Western medication group was by oral administration of Fluoxetine Hydrochloride capsules. The Hamilton Depression Scale (HAMD), National Institute of Health Stroke Scale (NIHSS), and Barthel Index (BI) were adopted to evaluate the depression degree, neural function deficit, and activities of daily living after 4-week and 8-week treatments. Result The total effective rate was 96.5% in the acupuncture group, versus 83.9% in the Western medication group, and the difference was statistically significant (P<0.05); the acupuncture group was superior to the Western medication group in improving the HAMD, NIHSS, and BI scores (P<0.01), and its therapeutic efficacy present with a feature of time-effect. Conclusion Xing Nao An Shen Jie Yu needling can significantly improve the symptoms of depression after cerebral stroke, promoting the recovery of neural function and enhancing the quality of life.