OBJECTIVE: To investigate the effect of Compound danshen dripping pills on cytochrome CYP1A2 activity for reference of clinical rational drug use. METHODS: The contents of four major metabolites of Caffeine, i.e. 5-acetylamino-6-formylamino-3-methyluracil(AFMU),1-methylxanthine(1X), 1-methyluric acid(1U) and 1, 7-dimethyluricacid (17U) in the urine before and after oral administration of Compound danshen dripping pills were determined by RP-HPLC. The activity of cytochrome CYP1A2 was derived from the ratio of (AFMU+1X+1U)/17U. RESULTS: The average activity of CYP1A2 was 4.20?1.54 before medication, which stood at 4.26?1.95 at 14 days and 4.35?1.26 at 28 days after medication, up 1.42% and 3.57%, respectively, yet there was no significant difference after medication as compared with before medication. CONCLUSION: No significant difference was noted on CYP1A2 activity after oral medication of Compound danshen dripping pills, nor does it modify the efficacy of the concurrent drugs that metabolized by CYP1A2.

Objective To understand the status quo of health education requirement of cervical cancer patients undergoing chemotherapy, in order to take individualized health education to improve the quality of life of patients, as well as provide basis for hospitals to conduct targeted health education. Methods 68 cases of cervical cancer patients with chemotherapy were investigated with health education questionnaire. Results Cervical cancer patients with chemotherapy were in general needs of health education, to understand the treatment protocol and effect came to the first place of strong demand. Patients with different times of hospitalization, different age had different emphasis point for health education needs. The most popular education style was oral education. Conclusions Cervical cancer patients with chemotherapy lack knowledge of health education, they hope to obtain health education about examination, treatment and rehabilitation. Nurses should give targeted, planned and individualized health education in order to meet the health needs of patients and improve their quality of life.

Objective To understand the epidemic situation,epidemiological characteristics and clinical features of import?ed malaria in Donghai County,Lianyungang City from 2005 to 2015. Methods The epidemiological data of imported malaria in Donghai County from 2005 to 2015 were collected and analyzed by the descriptive epidemiological method. Results A total of 126 imported malaria cases were reported in Donghai County from 2005 to 2015,including 101 falciparum malaria cases (80.19%),13 ovale malaria cases(10.32%),7 vivax malaria cases(5.56%),4 quartan malaria cases(3.17%),and 1 case with mixed infection(0.79%). These cases were distributed in 14 towns,and mainly in 4 towns namely Niushan,Tuofeng,Shil?iu,and Huangchuan,and the cases in the 4 towns accounted for 63.49% of the total cases. The cases occurred in the whole year,but the peak period of the disease was in April,which was related to the returned time of these patients from foreign coun?tries. All the patients were male and aged from 20 to 55 years,and those aged from 20 to 50 years accounted for 90.70%. Con?clusions The epidemic situation of imported malaria presents a rising trend in Donghai County in recent 10 years. To control imported malaria,the above?mentioned 4 towns with more migrant construction workers and businessmen should be treated as key areas. Meanwhile,the floating population management,disposal of the malaria epidemic spots,mosquito vector monitoring as well as health education should be strengthened.

Objective To investigate the rural medical orientation undergraduate student's professional commitment,time management disposition and learning burnout and the relationship between them. Methods Use the college students' learning Burnout scale,adolescence time management disposition Questionnaire and College Students'professional commitment scale,survey of Xinxiang Medical University grade 2010 ~2012,739 valid ques?tionnaires,the questionnaire has 92. 61% efficiency. Results Three scores were normally distribution. Learning burnout was the medium level,the average score of each entry was 2.88±0.45,and moderate and above level learn?ing burnout ratio was 45.06%. The average score of time management disposition was 3.45±0.43. Professional com?mitment level was higher,the average score was 3.82±0.43 for each item. Affective commitment,ideal commitment, the sense of time efficacy and the sense of time control have entered on the learning burnout of the stepwise regres?sion equation,and four variables together explained 44.0% of the variance in the dependent variable. Time manage?ment disposition,the professional commitment and learning burnout were negatively correlated, but between time management disposition and professional commitment is positively related, professional commitment, the variance explained by variables of learning burnout was 30.12%. Conclusion The rural medical orientation of undergradu?ate students time management disposition are important factors of learning burnout,which has the forecast function to the learning burnout. Professional commitment is part of the intermediary variables.

Traditional Chinese medicine exercise therapy has a long history in rehabilitation and keep in good health, which pays attention to integration of body and spirit.There are many clinical studies proving that it can improve heart function and quality of life in patients with cardiovascular diseases, and it is suitable for vigorously generalizing.

Objective:To prepare and characterize chitosan/hyaluronic acid (CS/HA) nanoparticles for miRNA-148b (miR-148b)delivery.Methods:CS/HA/miR-148b nanoparticles were prepared.The particle size,zeta potential,morphology were investigated by nano laser granulometer and TEM respectively.The encapsulation efficiency of CS/HA nanoparticles was evaluated by gel retarding analysis.The cytotoxicity and transfection efficiency of CS/HA/miR-148b nanoparticles on rat bone marrow mesenchymal stem cells (MSCs) were evaluated by CCK-8 assay,transfection assay and FCM respectively.Results:The CS/HA/miR-148b nanoparticles were discrete spherical particles with the diameter of 160 to 370 nm and zeta potential of + 15 to +41 mV.N/P ratio at 20∶1 of the nanoparticles showed the optimum encapsulation efficiency for miR-148b.CS/HA/miR-148b nanoparticles exhibited no cytotoxicity to MSCs and could deliver miR-148b to the MSCs at high transfection efficiency.Conclusion:CS/HA/miR-148b nanoparticles are safe and effective for miR-148b delivery into MSCs.

Objective:To investigate the protein expression levels of breast cancer metastasis suppressor 1 (BRMS1) and Con-nexin 43 (Cx43) in thyroid cancer and their correlation with clinicopathologic parameters. Methods:Immunohistochemistry Streptavi-din-Peroxidase method was used to detect the BRMS1 and Cx43 protein expression in 195 tissue samples, including 90 cases with thy-roid carcinoma, 45 cases with thyroid adenoma, 30 cases with nodular goiter, and 30 cases with normal thyroid glands. Results:The positive rates of BRMS1 and Cx43 expression was 56.7%(51/90) and 41.1%(37/90) in thyroid carcinoma, respectively, which are sig-nificantly lower than the rates in thyroid adenoma, nodular goiter, and normal thyroid gland tissues (P<0.001). Of the three pathological types of thyroid cancer, the positive expression rate of Cx43 was 61.9%in papillary carcinoma, 27.8%in medullary carcinoma, 27.3%in follicular carcinoma, and 12.5%in undifferentiated carcinoma. Statistical differences in the BRMS1 expression among papillary car-cinoma and the other pathological types were also noted. Unlike the patients without lymph node metastasis, the positive expression of BRMS1 and Cx43 proteins were both significantly low among patients suffering from nodal metastasis. Subgroup analysis shows that the positive expression of BRMS1 and Cx43 protein gradually decreased with TNM staging. In addition, a positive correlation was ob-served between the BRMS1 and Cx43 protein expression (r=0.494, P=0.032). Conclusion:The decreased expression of BRMS1 and Cx43 proteins is significantly correlated with the metastasis of thyroid cancer and malignant grade. The combined detection of the two proteins can be ideal biomarkers for judging the prognosis of thyroid carcinoma.

BACKGROUND:The incidence rate of cervical abnormalities of the patients with skeletal class Ⅱ and normal occlusion has been studied abroad, but the researches on the incidence of cervical abnormalities in different malocclusion patients is rare at home. OBJECTIVE:To observe the imaging of patients with cervical abnormalities and to statistics the incidence of cervical abnormalities in different malocclusion patients through analyzing the lateral cephalogram of different malocclusion patients. METHODS:Skeletal class Ⅱ group was consisted of 93 patients, 41 male (aged 18-40 years) and 52 female (aged 22-35 years), with the A point-nasion-B point angle>5°. Class Ⅰ group was consisted of 45 patients, 31 female (aged 20-36 years) and 14 male (aged 17-38 years), with the 1°

Oral anticoagulants are important for preventing stroke in patients with atrial fibrillation.Compared with the traditional oral anticoagulant warfarin,the novel oral anticoagulants (NOACs) have the characteristics of high efficiency,safety,and no need to monitor coagulation function.However,current clinical reports have showed that the dose is usually low when NOACs were used for preventing stroke in patients with atrial fibrillation.Its main reason may be associated with the risk avoidance and the difference in doctor and patient preferences.The analysis from the aspects of safety and effectiveness,the risk of ischemic stroke or systemic embolism is lower when the dose of NOACs is relatively high,and the risk of hemorrhage is lower when the dose of NOACs is relatively lower.Given the differences between the incidences of different events,the disability rate and the mortality rate,the patient with atrial fibrillation are more suitable for using high-dose drug.When choosing a specific dose,taking into account the specificity,the appropriate dose,intensity,and dosing regimen should be given according to the guideline recommendations,appropriate reference to renal function and patient preferences,and individual differences in order to obtain the best clinical efficacy..

The pneumoperitoneum may reduce the renal blood flow which was demonstrated both in experimental and clinical research. Greater change could be seen as the pressure of pneumoperitoneum increased,which may induce lower GFR, decrease urine output ,increase the level of plasma Scr?BUN and so on.All these changes were related to the variety of hemodynamics?nerval-endocrine factors releasing?body position and sorts of insufflated gas.Ischemia-reperfusion injury also is an ignorable factor. Pneumoperitoneum may influence the kidney function , which is reversible under certain pressure and operating time,and were concerned with multiple mechanism. More obvious damage to the dysfunctional kidney could occur under high pressure of pneumoperitoneum.