OBJECTIVETo investigate necessity of the further processing for adsorptive resin material.

METHODSAccording to GB/ T 16886.4, coagulation tests, haematology tests, in vitro spontaneous platelet-adhesion test and complement activation tests were conducted respectively to assess the in vitro blood compatibility of adsorptive resin material.

RESULTSIn comparison with polypropylene, he adsorptive resin material appears to be a very slight activator to the endogenous blood coagulation system, it can inhibited the exogenous blood coagulation system very significantly (P < 0.01), it can adhere to fibrinogen, red blood cells, reticulocytes very significantly (P < 0.01), and platelet significantly (P < 0.05). The adsorptive resin material is also revealed to be a very significant activator (P < 0.01) to the complement system.

CONCLUSIONSThe in vitro blood compatibility assessment witnesses that it is very necessary for the adsorptive resin material to go through the certain special processing(such as the surface modification and the microencapsulation technology, etc) before its application to human being.

Objective To measure the sizes of the left and right internal jugular veins (IJV) and transverse sinuses (TS) with three dimensional contrast‐enhanced MRA(3D CE‐MRA) ,and discuss the correlation of cerebral venous sinus and IJV .Methods One hundred and ten patients without vasculopathy and blood flow abnormalities were selected for 3D CE‐MRA ,55 cases for each gender .The images were taken three‐dimensional reconstruction on work‐station by different software .The normal IJV and TS was measured and the statistical comparison between the bilateral IJV and TS was analyzed .Results (1)The inside diameter of IJV re‐duced in turn at the measurement point of J1 ,J2 and J3 .The internal diameter of the right side of IJV was broader than left one ,and there was significant difference between the size of left and right side (P<0 .05) in these measurements .The internal diameter of the right side of TS was broader than left one ,there was difference between the sizes of the left and right of TS (P<0 .05) .(2)The proportion of R>L ,R

Objective:To discuss the diagnosis,treatment of uterus cornea pregnancy.Methods:Retrospective analysis was made in 21 cases of uterus cornua pregnancy is recent 10 years.Results:8 cases were diagnosed uterus cornua,9 cases were misdiagnosed ectopic pregnancy,1 case was misdiagnosed rupture of uterus of artifical obortion,1 case was misdiagnosed pregnancy with uterus septus,1 case was misdiagnosed choriocarcinoma.Surgical treatment was done in 19 patients,and the other 2 cases received conservative therapy.Conclusion:Cornual pregnancy is often misdiagnosed as ectopic pregnancy or other diseases.More careful gynecological and transobdominal ultrasound examination will improve the accuracy of diagnosis.

Objective To investigate expression and significance of matrix metalloproteinase-9 (MMP-9) and neutrophil gelatinase-associated lipocalin (NGAL) in serum and placental tissue of preeclampsia maternal.Methods 74 cases of preeclampsia pregnant women as research subjects,which 39 cases of severe preeclampsia,35 cases of mild preeclampsia,selected 30 cases of normal late maternal as the control group.Three groups of maternal serum levels of MMP-9 and of NGAL were detected by enzyme-linked immunosorbent assay.RT-PCR was used to test placenta MMP-9 mRNA,and NGAL mRNA level.Results Preeclampsia maternal serum levels of MMP-9 and NGAL level were significantly higher than those of severe preeclampsia pregnant women serum level of MMP-9 and NGAL were significantly higher than mild preeclampsia (t =3.127,2.846,3.508,3.284,all P ＜ 0.05).Preeclampsia placentas of MMP-9 mRNA and NGAL mRNA levels were significantly lower than the control group (t =-2.842,-2.728,-3.532,-3.276,(a)ll P ＜ 0.05),severe preeclampsia placentas of MMP-9 and NGAL were significantly higher than mild preeclampsia(t =2.732,2.741,all P ＜ 0.05),severe preeclampsia placentas of MMP-9 mRNA and NGAL mRNA levels were significantly lower than in patients with mild preeclampsia (t =-2.681,-2.587,all P ＜ 0.05).Preeclampsia maternal serum levels of MMP-9 and NGAL was positively correlated with placental tissue mRNA levels of MMP-9 and NGAL mRNA level was positively correlated (P ＜ 0.05).Conclusion Preeclampsia maternal serum MMP-9 and NGAL were significantly elevated,placental MMP-9 mRNA and NGAL mRNA levels were significantly decreased,suggested that they may be involved in the pathogenesis of preeclampsia.

In this work, rare specific binders to botulinum neurotoxin antigen were selected from human phage immunized library through three rounds of panning in vitro. Of them, clone ScFvB17 is of high affinity and specificity to target antigen. B17 is of 750bp encoding 250 amino acid. Analysis results of gene structure showed V-genes of B17 belonging respectively to VH4 or? chainⅡ family are new antibody Fv genes. More important result showed that recombinant ScFvB17 expressed in E.coli could bind specific for neurotoxin competing with antiserum. Successful selection of human phage antibody library and obtaining specific ScFv against botulinum neurotoxin serotype A provide useful materials for further study on detection of neurotoxin and human engineering therapeutic antitoxin.

OBJECTIVE To understand infection status and drug resistance of Ureaplasma urealyticum(Uu) and Mycoplasma hominis(Mh) in female genitourinary tract in the local region.METHODS The results of mycoplasma cultivation and susceptibility tests of female vaginal secretions specimen in 409 cases during from Sep 2007 to Apr 2008 were analyzed retrospectively.RESULTS Among 409 cases,the positive rates were mycoplasma cultivation(57.2%).Uu(72.6%),Mh(3.4%) and combined infection(23.9%).Drug resistance of Uu to ten kinds of common antibiotics showed that minocycline

[Objective] To evaluate the efficacy and safety of quadruple therapy based on moxifloxacin and bismuth in the treatment of Helicobacter pylori eradication failure.[Methods] A prospective,randomized,comparative study was conducted in which 136 eligible patients were randomly divided into the extended quadruple group (66 patients,and the quadruplex therapies are Pectin bismuth 100 mg tid + lansoprazole 30 mg bid + amoxicillin 1000 mg bid + moxifloxacin 400 mg qd) and the classical quadruple group (70 patients,and the quadruple therapies are colloidal pectin bismuth 100 mg tid+ lansoprazole 30 mg bid +tetracycline tablet 750 mg Bid + metronidazole tablets 400 mg bid combination) and were under treatment for 14 days.One month after the treatment,endoscopy,rapid urease test,and 13-carbon/ 14-carbon urease breath test were used to evaluate the adverse effects and adherence of the drugs in the two groups respectively.[Results] In the extended quadruple group,among 60 patients who had completed the final treatment and follow-up survey,55 were successfully treated,with eradication rates of (83.3％,55/66)and 91.7％ (55/60) according to intention-to-treat (ITT) and Per-protocol (PP) respectively.In the classic quadruple group,64 patients completed the final treatment and follow-up survey.Among them,37 cases were successful,and the eradication rates according to ITT and PP remedy are 52.9％ (37/70) and 57.8％ (37/64) respectively.The difference was statistically significant (P ＜ 0.001).Adverse drug reactions and compliance monitoring showed no significant difference between the two groups (P ＞ 0.05).[Conclusion] The quadruple therapy based on moxifloxacin and bismuth displays a higher eradication rate than that of the classical bismuth quadruple therapy on the first-time elimination failure of Helicobacter pylori while shows no difference in safety consideration,thus can serve as remedial therapy for the primary Hp eradication failure.

In this paper, some study methods of pharmacokinetic, the explanation ways of data and experimental methods of transdermal therapeutic system(TTS) were described. The advancement of modern science accelerates the development of pharmacokinetic of TTS and makes more progress in this field.

Objective To study the clinical value of procalcitonin on early diagnosis of sepsis .Methods 60 patients who entered ICU were enrolled in the present study were chosen .The change of procalcitonin , CRP, WBC,ESR,GRANR and the highest temperature was observed dynamically with 1 day and 4 days in hospitalization . The patients were ultimately divided into the two groups according to the diagnostic standard of sepsis ,the sepsis group (SIRS group) and infective systemic inflammatory response syndrome group (NONSIRS group).Results After ana-lyzing the processed data,researchers found that PCT of the sepsis group (6.73 ±1.31)ng/mL was higher evidently than that of the SIRS group(0.25 ±0.05)ng/mL(t=3.10,P<0.05),the area under ROC curve of PCT (0.869) is evidently greater than CRP(0.798),WBC(0.737) and ESR(0.394)(P<0.05),which had statistical significance. Conclusion Procalcitonin is a good index of early diagnosis on sepsis ,therefor,its sensitivity and specificity is higher than the other inflammatory indicators .Finally,procalcitonin can be widely used in clinical trial .