Objective To evaluate the effect of color Doppler ultrasound monitoring in artificial arteria-venous fistula in patients with uraemia.Methods 25 cases were examined with upper limbs arteriovenous fistulas with uremia and analysed by CDFI and 2D.The fistula and blood vessel was observe and hemodynamics changes were examined.Results Among 25 patients,18 cases showed good filling state of the vessel,3 cases with anastomoses stenosis,2cases with thrombus and 2cases with venous dilatations.Conclusion CDFI is an effective supplementary detective methods in treating hemodialysis patients with uremia.

Objective To explore the influence of ICAM-1 and LFA-1 on the breast cancer metastasis and provide scientific base for studying the molecular mechanism of breast cancer metastasis by observing the expression of ICAM-1(intercellular adhension molecule1) and LFA-1(Lymphocyte function related antigen 1) in different time different metastasis stage of breast cancer. Methods We observed the expression of ICAM-1 and LFA-1 in breast cancer tissue as well as the expression of ICAM-1、LFA-1 and VEGFR-3 in Lymphatic vessel endothelial cell of nearby the breast cancer tissues from the patients of the operation in the defferent stages with the methods of HE staining and immunohistochemistry staining. Results The positive rate of ICAM-1 and LFA-1 expression in cancer cell and cancer nest of breast cancer was increased gradually along with the increase of clinical stages and the occurrence of lymph node metastasis. And the positive rate of ICAM-1、LFA-1 and VEGFR-3 expression in lymphatic vessel endothelial cell of nearby the breast cancer tissues was increased gradually too along with the increase of clinical stages and the occurrence of lymph node metastasis,which has the positive correlation,and it has the positive correlation that the ICAM-1 and LFA-1 expressed in Lymphatic vessel endothelial cell of nearby the breast cancer tissues(P0.05).Conclusion The ICAM-1 and LFA-1 were related with breast cancer lymph metastasis,and the ICAM-1 and LFA-1 promoted breast cancer lymph metastasis through synergism each other.The increase or decrease of ICAM-1 and LFA-1 expression in the breast cancer tissue and lymphatic vessel endothelial cell of nearby the breast cancer tissues could as the data target to display certain instruction function for prevention and treatment of breast cancer lymph metastasis.The Lymphatic vessel quantity of nearby the breast cancer tissues was increased along with tumor progress, which may be related with breast cancer lymph metastasis.VEGFR-3 has the high specificity in the lymphatic vessel endothelial cell expression,and may act as the lymphatic vessel as the lymphatic vessel endothelial cell specific marker.

The effect of CHIP [cis-dichloro-trans-dihydroxy-bis-isopropy-lamine platinum (Ⅳ)] on the biosynthesis of DNA, RNA, and pro-tein of Ehrilch ascites carcinoma ( EAC ) cells in vitro was studied. 3H-TdR, 3H-UR, 3H-Leu were used as precursors of cellular macro-molecules to study the effect of CHIP on the biosynthesis,with CDDP [cis-dichlorodiammineplatitinum (Ⅱ)] as a positive control. The experimental results showed that CHIP, similar to CDDP, inhibited the biosynthesis of DNA, RNA and protein, and has a strong antitumor activity. The inhibitory effect on DNA biosynthesis lasted 40 hours indicating that CHIP might induce permanent damage in DNA template and thus reduced the mitotic division of tumor cells. Furthermore, in contrast to the general view, the in vitro experimental results showed that CHIP, a tetravalent platinum coordination compound, exerted its antitumor activity directly in vitro.

Objective To study the chemical constituents in the immature fruits of Momordica charantia. Methods Isolation and purification were carried out by macroporous absorption resin and silica gel, and compounds were identified and elucidated by spectral and chemical methods. Results Ten compounds were obtained from alcohol extract and five of them were determined as germanicyl acetate (Ⅰ), aglycone of momordicoside Ⅰ (Ⅱ), aglycone of momordicoside L (Ⅲ), charantin (Ⅳ), and ?-sitosterol (Ⅴ). Conclusion For all the first time, compound Ⅰ is found in the plants of Momordica L., compound Ⅱ is found as novel natural product in this plant, and compound Ⅲ is found in China.

BACKGROUND:Although tumor stem cel s and their differentiated vascular cel s, which are not sensitive to chemotherapy and molecular targeted therapy, reduce overal blood supply of the tumor, these cel s facilitate the invasion and metastasis of the tumor, eventual y resulting in treatment failure. OBJECTIVE:To investigate the effect of gastric cancer stem cel s in stomach cancer invasion and metastasis as wel as its mechanism in angiogenesis. METHODS:A total of 20 gastric cancer stem cel s and 20 normal gastric cancer cel s SGC7901 were cultured in the medium with DMEM/F12 (1:1), 2%B27 and 20μg/L epidermal growth factors or cultured in 10%PBS, respectively. Invasion of gastric cancer stem cel s was detected by sphere formation assay, colony formation assay and Transwel invasion assay. Levels of mRNA and protein expression of CD44 and E-cadherin were measured to observe tumor metastasis. Besides, angiogenesis was observed by cel scratch test, Transwel migration assay and ring test. RESULTS AND CONCLUSION:Gastric cancer cel s adhered to the medium wal , and gastric cancer stem cel s suspended in the medium, which appeared to be spindle interstitial form. Number of gastric cancer tumor spheres was significantly lower than that of gastric cancer stem cel spheres in the sphere formation assay and colony formation assay (P<0.05). Number of gastric cancer stem cel s through the basement membrane was significantly higher than that of gastric cancer cel s (P<0.05). And the cel migration distance and ring number were significantly increased in the gastric cancer stem cel s as compared with the gastric cancer cel s (P<0.05). In conclusion, the invasion, migration and angiogenesis abilities of gastric cancer stem cel s are stronger than those of gastric cancer cel s, which can further promote the occurrence and progression of gastric cancer.

Objective To explore the therapeutic mechanism of Xiao Chaihu Decoction for endometriosis in rats. Methods The rat models of endometriosis were treated with Xiao Chaihu Decoction, Danazol, and Xiao Chaihu Decoction plus Danazol for 4 weeks,respectively. And then cyclooxygenase-2(COX-2) expression in endometrium and endometriotic tissue was observed by immunohistochemistry method. Results The expression of COX-2 in the endometriotic tissue was lower than that in endometrium in Xiao Chaihu Decoction group and Xiao Chaihu Decoction plus Danazol group, while the results in Danazol group and control group were opposed to those in the above two groups. Conclusion The mechanism of Xiao Chaihu Decoction for rats endometriosis may be related to the decrease of COX-2 expression in ectopic Endometrium, thus reducing the formation of prostaglandins.

Objective To investigate the changes of plasma bone morphogenetic protein-4 (BMP-4) levels in patients with coronary heart disease (CHD) and rosuvastatin intervention effect on BMP-4 level.Methods Fifty-two patients with CHD and 35 health people were enrolled in this study as CHD group and control group.ELISA method was used to detect the concentration of plasma BMP-4.Analyzed the relationship between plasma BMP-4 and blood lipids,flow-mediated dilation (FMD),nitric oxide (NO),cyclooxygenase-2 (COX-2),malondialdehyde (MDA) and superoxide dismutase (SOD).And observed the changing of plasma BMP-4 before and after rosuvastatin intervention.Results Plasma BMP-4 level in CHD patients was (7.53 ± 1.20) μg/L,higher than that of control group ((3.81 ± 0.79) μ g/L,t =3.541,P =0.006).After rosuvastatin treatment,plasma BMP-4 level in CHD patient was decreased from (7.53 ± 1.20) μg/L to (5.40± 0.98) μg/L (t =1.436,P =0.001).Plasma BMP-4 level was positively correlated with COX-2,MDA,low-density lipoprotein cholesterol,total cholesterol (r =0.395,0.350,0.274,0.288 respectively,P ＜ 0.01 or P ＜0.05).But,it was negatively correlated with NO,high-density lipoprotein cholesterol,SOD,FMD (r =-0.291,-0.253,-0.476,-0.320 respectively,P ＜0.01 or P ＜0.05).COX-2,SOD and FMD were independent risk factors of plasma BMP-4 in patients with CHD.Conclusion Oxidative stress and endothelial dysfunction are in patients with CHD.Rosuvastatin treatment can remarkably reduce plasma BMP-4 level,alleviate vascular endothelium injury induced by oxidative stress and improve endothelial function in patients with CHD.

OBJECTIVE:To study and analyze the impact of rosuvastatin on function changes of endothelial cell. METHODS:30 patients with coronary heart disease were selected from our hospital during Jun. 2013 to Jun. 2014,and 15 healthy persons were included. They were divided into healthy control group,drug intervention of coronary heart disease group and coronary heart disease group with 15 cases in each group. The levels of asymmetric dimethylarginine (ADMA),NO and nitric oxide synthase (NOS) were determined by ELISA and compared among those groups. Its relationship with OD value was analyzed. After cultured for 4 h and 8 h,human umbilical vein endothelial cells (HUVEC) were divided into 5 groups,i.g. control group 0 μmol/L,0.1 μmol/L group,1 μmol/L group,10 μmol/L group,100 μmol/L group. The change of DDAH mRNA expression in endothelial cells treated with different concentrations of rosuvastatin was detected. RESULTS:The level of ADMA in drug intervention of coronary heart disease group were significantly lower than in coronary heart disease group,but still higher than that in healthy control group;NO and NOS levels were significantly higher than coronary heart disease group,but still lower than healthy control group,with statisti-cal significance (P<0.05). According to Pearson method correlation analysis,OD value was positively correlated to ADMA,NO and NOS. 4h after HUVEC culture,RT-PCR showed that mRNA expression of DDAH were increased in 0.1 μmol/L,1 μmol/L, 10μmol/L and 100μmol/L rosuvastatin groups,there was statistically significant difference between 10μmol/L and 100μmol/L ro-suvastatin groups and control group (P<0.05). 8 h after HUVEC culture,RT-PCR showed mRNA expression of DDAH were in-creased in 0.1 μmol/L,1 μmol/L,10 μmol/L and 100 μmol/L rosuvastatin groups,and there was statistically significant difference between 10 μmol/L rosuvastatin group and control group(P<0.05). CONCLUSIONS:Rosuvastatin is a new type of lipid-lowering drugs,which has a good protective effect on endothelial cells,it is worthy of attention in the clinic.

Objetive To analyze the standard of training and evaluation on semi-automatic biochemical analyzer to improve grass-root medical unit in training on the analyzer.Methods The standard was analyzed and expounded from the aspects of scope of application,requirements,subjects setting,key links as well as the demands of evaluation scoring scale.Results The main points of the standard included elementary knowledge of biochemical analysis as well as the structure,principle,installation,operation,application,maintenance and etc of the semi-automatic biochemical analyzer.The integration of personnel and instrument contributed to accurate and rapid output of test report.Conclusion It is necessary to grasp accurately the connotation of the standard to improve the quality of medical equipment training.

Objective To investigate the effect of curcumin on chronic pancreatitis in rat.Methods Eighteen one-month old male SD rats were randomly divided into control group,ANP group and curcumin group with 6 rats in each group.After fed by 25％ alcohol for twelve weeks,the rats in ANP group were intraperitoneally injected with lipopolysaccharides ( LPS,2 mg/kg body weight) once a week for 4 weeks.The rats in curcumin group were fed with curcumin when they were intraperitoneally injected with lipopolysaccharides.Seventeen weeks later,the rats were sacrificed.Serum glucose was measured,pancreatic morphology was routinely examined,collagen deposition was determined by Masson stain,and the amylase and lipase in pancreatic tissue were evaluated,TGF-β1 mRNA was determined by using RT-PCR.Results The pathological score of control group,ANP group and curcumin group was 1.17 ±0.41,7.33 ±2.58 and 3.50 ± 1.05,and the score in curcumin group was lower than that of ANP group (P ＜ 0.01 ),but which was higher than that in control group (P ＜ 0.05 ).There was no difference in the serum glucose among the groups.The levels of amylase and lipase in pancreatic tissue were (7348 ± 102 ),( 14135 ± 1272) U/g,which were significantly higher than those in ANP group[( 5428 ± 547 ) and (9123 ± 1250 ) U/g,P ＜ 0.05].The expressions of TGF β1 mRNA in control group,ANP group and curcumin group were 0.618 ± 0.019,0.818 ±0.012,0.745 ±0.088,and the values in ANP group were significantly higher than those in control group and curcumin group (P ＜ 0.01 ).Conclusions On the basis of long term ethanol intake,repeatedly intraperitoneal injection of LPS can induce chronic pancreatitis,and curcumin has anti-fibrosis effect possibly by its inhibitory effect on the expression of TGF-β1.