Objective To explore the treatment of gestational diabetes mellitus (GDM) and the selection of time and pattern for termination.Methods A prospective study on 40 cases of patients with GDM was carried out.The patients' clinical data were analyzed by statistical method.Results There was a significant difference in the neonatal suffocation between insulin treatment combined with diet limitation and diet limitation alone(P0 05). Compared with 37 to 40 gestational weeks, neonatal morbidity obviously increased in 40 gestational weeks (P

BACKGROUND: Theoretically, a novel single-sided groove target release biodegradable stent can avoid drug-induced inflammation of the local vessel wall, reduce drug dose, decrease the likelihood of thrombosis, and relieve stenosis.OBJECTIVE: To evaluate the influence of the novel single-sided groove target release biodegradable stent on intimal hyperplasia in a healthy porcine coronary artery model.METHODS: Eighteen healthy pigs were randomly divided into three groups, bare metal stent group, Firebird metal stent group and Firehawk stent group, which were implanted with L605 cobalt chromium alloy bare metal stents and L605 cobalt chromium alloy non-biodegradable rapamycin-eluting stent and L605 cobalt chromium alloy biodegradable rapamycin-eluting stent, respectively. Two same stents were implanted into the left anterior descending artery and the right coronary artery of each pig, respectively. After 1 and 3 months of follow-up, coronary angiography was performed,and the pigs were killed to take coronary artery samples for detection of intima hyperplasia.RESULTS AND CONCLUSION: At 1 and 3 months after stent implantation, no stenosis, thrombosis and aneurysm occurred in the three groups shown by the coronary angiography; the lumen loss was significantly lower in the Firebird metal stent group and Firehawk stent group than the bare metal stent group (P < 0.05), while there was no difference between the Firebird metal stent group and Firehawk stent group. Histopatologically, there was no coronary arterial tissue necrosis or luminal thrombosis in the three groups. Compared with the bare metal stent group, at 1 and 3 months after stent implantation, the neointimal area and the percentage of stenosis were significantly lower in the Firebird metal stent group and Firehawk stent group (P < 0.05), while the residual lumen area and internal elastic lamina area were significantly higher (P < 0.05). Additionally, there was no significant difference between the Firebird metal stent group and Firehawk stent group. Overall, the Firehawk stent that achieves the same clinical efficacy as the non-biodegradable rapamycin-eluting stent effectively inhibits intimal hyperplasia, and prevents stent restenosis after stent implantation.However, future investigations on the long-term effect are warranted.

Objective To study the relationship among hydrops fetalis(over 28 gestational weeks),Mirror syndrome and preeclampsia(PE). Methods Clinical data of 13 cases of hydrops fetalis were analyzed and relative publications were reviewed. Results Eight out of the 13 cases were diagnosed as PE,and 5 were Mirror syndrome.Serious maternal complications occurred in all cases with either Mirror syndrome or PE. ConclusionsFetaland placental hydrops was closely related with maternal symptoms.Clinicians should be aware of this condition and detect Mirror syndrome in time so as to prevent its progress to PE.However,the pathogenic mechanism remains to be fully elucidated.More attention should be paid to both mothers and the hydrops fetalis.

Adult ; Aged ; Chlorine ; poisoning ; Female ; Follow-Up Studies ; Humans ; Lung ; physiopathology ; Male ; Middle Aged ; Young Adult

Objective To investigate of human esophageal squamous carcinoma cells(Eca-109) in which cyclooxygenase (COX)-2 gene was knocked down by adenovirus delivered siRNA.Methods Based on the plasmid pSUPER cloned with RNA polymerase Ⅲ-dependent promoter HI,the interfering plasmid psiRNA/COX-2 targeting human COX-2 mRNA was constructed,The siRNA/COX-2 fragment was derived from psiRNA/COX-2 digested by Not I and Xho 1.and was cloned into the shuttle plasmid pAdTrack.Then pAdTrack/siRNA/COX-2 was obtained and co transfected into the E.coli strain BJ5183 with the bone plasmid pAdEasy-1,the recombinant adenovirus Ad/siRNA/COX-2 was generated by homologous recombination.Having been packaged and amplified in cells 293,Ad/siRNA/COX-2 was transfected into Eca-109 cells.The PGE2 concentration in the cells culture supernatant was determined by ELISA,and the level of COX-2 mRNA in the cells was tested by real time PCR.Moreover,cell cycle and apoptosis were determined by flow cytometry,and cells growth curve was protracted.Results The recombinant adenovirus Ad/siRNA/COX-2 was successfully constructed.Ninty six hours after Ad/ siRNA/COX 2 transfecting into Eca 109 cells,COX-2 mRNA was reduced by 71.7%,and PGE2 concentration in the cells culture supernatant was decreased by 62.0%.Correspondingly,the growth of cells slowed down.At the same time,the cells in G0-G1 phase was increased by 32.24%,and those in S phase and G2-M phase were reduced by 16.38% and 15.86%.respectively.And cells apoptosis index was increased by 9.19%.Conclusion The adenovirus based-RNAi was capable of knocking down remarkably COX-2 of human esopbageal carcinoma cells,which lead to growth of cells slowing down.

Humans ; Medicine, Chinese Traditional ; Syndrome ; Temperature

This paper introduces a microtiter-plate scanning instrument for medical laboratory equipment,which is controlled by the embedded PC when moving along the X direction and Y direction on the microtiter-plate plane. The designs of the driving circuit and orienting circuit are included in this paper. This instrument has been used in PKU fluorescence laboratory equipment for the newborn baby.

Objective To study the mechanism of protective effects of total rhizoma panacis japonica saponins(tRPJS)on the cerebral ischemia injury.Methods The middle cerebral artery occlusion model (MCAO)in rats and cerebral ischemia-reperfusion models in mice were used to investigate the influence of tRPJS on the nitric oxide synthase(NOS)and inducible nitric oxide synthase(iNOS)activity in hippocampus region.Results tRPJS significantly decreased the contents of NOS and iNOS in hippocampus region of MCAO rat and cerebral ischemia reinfusion mouse.Conclusion tRPJS has significantly protective effects by decreasing NOS and iNOS.

Objective To investigate the roles of iron ion in the development of experimental hydrocephalus in rats. Methods Seventy-five Sprague-Dawley female rats were divided into 3 groups randomly,that is,the iron group,the semi-iron group and the artificial cerebrospinal fluid (CSF) control group. FeC13 of 5 ?l dissolved in artificial CSF with the concentration of 6.67 g/L and 3.33 g/L were slowly infused into the right lateral cerebral ventricle of the rats in the iron group and in the semis-iron group respectively. The rats in the artificial CSF control group were infused 5 ?l artificial CSF. The rats were sacrificed 1,14 and 28 d later to measure the transverse diameter of the lateral ventricle for the evaluation of hydrocephalus and the index of ventricle,and pathology of brain was also observed. Results There were significant difference of hydrocephalus between the iron group (9/10) and the semis-iron group(1/5) at 28 d (P