Mammalian target of rapamycin (mTOR) signaling pathway,one of the most important cellular signaling transduction pathways,regulates cell survival,proliferation,transdifferentiation,migration and cell cycle by influencing the activation state of various effector molecules in the downstream.Abnormal regulations of these machanisms are closely related to the tumorigenesis and progression of colorectal cancer (CRC).Currently,mTOR inhibitors have been approved in clinical trials of CRC and have made some progress.

By analyzing the Military Health System winning "Heliang & Heli" Fund Award between 1994 and 2009,we found that the Military Health System possessed a good scientific basis and high level,but with an unbalanced scientific research development,which suggests the Military Health System should further strengthen its independent innovation ability.

Soluble ST2 (sST2) is a decoy receptor of IL-33 in blood.sST2and ST2L (receptor on cell membrane) seem to be markedly induced in mechanically overloaded cardiac myocytes,especially the expression of sST2.A large number of sST2 bind IL-33 thus subtracting IL-33 from the interaction with ST2L,potentially attenuating the cardioprotective effects of IL-33/ST2L pathway.Recently,sST2 has emerged as a novel for heart failure biomarker.Though sST2 was a less robust marker for the diagnosis of heart failure than NT-proBNP,it has been implicated in the prognostication of patients with acute dyspnea,acute or chronic heart failure and myocardial infarction,with particular value for mid-and long-term prognosis.However,further studies are needed in order to better point out the evidence for a routine use of sST2 evaluation in patients suffering from cardiovascular diseases.

Granulocyte colony-stimulating factor is a glycosylated polypeptide chain cell growth factor that can promote proliferation and differentiation of myeloid progenitors and induce maturation of these cells toward terminally differentiated neutrophils.Granulocyte colony-stimulating factor which functions and effects depend on the combination with specific receptor on the surface of effector cells.With the development of gene cloning technology,ganulocyte colony-stimulating factor recombinant products have been widely used in clinic to provide effective means for the treatment of leucopenia,In recent years,we find the therapy of chemotherapeutics combining with granulocyte colony-stimulating factor is effective in refractory/relapsed/elderly cases of acute leukaemia.Currently,granulocyte colony-stimulating factor is one of important and successful hematopoietic cytokines in clinic.

Objective: To explore therapeutic effect of individualized cognitive training on improving cognitive function in aged patients with coronary heart disease (CHD) after percutaneous coronary intervention (PCI).Methods: A total of 92 aged CHD patients after PCI, who were diagnosed and treated in our hospital, were selected.According to random number table, they were randomly and equally divided into routine nursing group (n=46) and cognitive training group (n=46, received active individualized cognitive training based on routine nursing), both groups were observed for three months.Scores of Montreal cognitive assessment (MoCA), self-rating anxiety scale (SAS) and self-rating depression scale (SDS), and left ventricular ejection fraction (LVEF) were compared between two groups before and after nursing.Results: Compared with before nursing, after nursing, there were significant rise in LVEF and MoCA score, and significant reductions in scores of SAS and SDS in both groups (P<0.01);compared with routine nursing group, there were significant rise in LVEF [(51.87±4.89)% vs.(58.29±5.13)%] and MoCA score [(24.44±4.10) scores vs.(29.34±3.14) scores], and significant reductions in scores of SAS [(38.22±5.64) scores vs.(32.09±4.11) scores] and SDS [(37.22±4.98) scores vs.(30.56±5.68) scores] in cognitive training group, P<0.01.Training compliance of cognitive training group was significantly higher than that of routine nursing group (95.7% vs.82.6%, P=0.044).Conclusion: Individualized cognitive training can significantly improve training compliance after PCI, significantly improve cognitive function, relieve anxiety and depression, and enhance cardiac function in aged CHD patients.

Malignant hyperthermia is a rare autosomal-dominant genetic disorder. This article would review the advance in the researchs of malignant hyperthermia, including its incidence, pathogenesis and diagnosis.

OBJECTIVE:To investigate the feasibility of defined individual treatment course as evaluation index of rational use of drugs.METHODS:The doses of antibiotics and treatment course in 63 patients with type Ⅰ incision operation of our hospital in Jan.2010 were taken as examples.Case analysis,DUI evaluation and defined individual treatment course evaluation were adopted.Similar results were obtained by 3 kinds of methods.RESULTS&CONCLUSION:Results of 3 kinds of methods are consistent.DUI evaluation isn't closely associated with treatment course.Defined individual treatment course is more sensitive and available than DUI based on the introduction of dose and treatment course.

Arsenic ; blood ; urine ; Environmental Monitoring ; methods ; Humans ; Spectrometry, Fluorescence ; Spectrophotometry, Atomic

Objective To explore efficacy and mechanism of recombinant human granulocyte-colony stimulating factor(rhG-CSF)in treatment of different leukopenia.Methods A total of 50 patients were divided into 4 groups:aplastic anemia(AA)group(n=10),myelodysplastic syndromes(MDS)group(n=10),drug-induced leukopenia group(n=20)(subdivided into hyperplasia group and hypoplasia group by the degree of cellularity)and iron deficiency anemia group(control group,n=10).The concentrations of G-CSF of peripheral blood were measured by ELISA method.The ratio of CD34+ cells and G-CSFR expression in blood marrow mononuclear cells(BMMNC)were measured by flow cytometry.Granulocyte colony-forming units(CFU-G)of MNC were cultured.Clinical efficacy of rhG-CSF to all patients were determined.Results The concentrations of G-CSF were higher in AA and drug-induced leukopenia groups than those of MDS and control groups(P0.05).Expressions of G-CSFR were lower in hypoplasia group than those in hyperplasia and control groups(P0.05).The ratio of CD34+ cells was lower in AA group than that of other groups(P0.05).Concentrations of CFU-G were lower in AA and MDS groups than those in drug-induced leukopenia and control groups(P0.05).The efficacy of rhG-CSF was superior in drug-induced leukopenia group than in MDS group,and superior in MDS group than in AA group(P

Objective: To explore the biological properties of keratinocytes from differently-aged healthy human beings. Methods: Keratinocytes from fetus, teenager and middle-aged groups were separated and cultured. The population doubling time (PDT) and cell growth curve in different cells were compared, and the cell cycles were analyzed by flow cytometry. Results: Circled digit one In primary culture of keratinocytes, the adherence time in middle-aged group was longer than that in fetus and teenager groups. However, all cell morphology showed no obvious differences. In subculture of keratinocytes, with donator's age increasing, time of cell adherence prolonged, passage number decreased and differences in cell morphology were obvious. Circled digit two The average PDT of keratinocytes was shorter in fetus group than in teenager and middle-aged groups. But difference in cell growth curve between different passages was not observed. Circled digit three Keratinocytes showed G2/M period in fetus group but G0/G1 period in teenager and middle-aged groups mainly. Conclusion: As age increases, the biological properties of keratinocytes change obviously.