The studies of animal models have shown that the expression of matrix metalloprotease (MMP) is abnormal during cerebral ischemia/reperfusion,which indicating that it is associated with cerebral ischemia/reperfusion injury,especially MMP-2,MMP-3 and MMP-9 play the important roles in the pathogenesis of acute ischemic cerebrovascular disease.Understanding of the roles and expressions of MMP,the factors affecting its expression,and the studies and application of the related antibodies in cerebral ischemic/reperfusion injury may provide a new alternative for the early diagnosis,prevention and treatment of cerebral infarction.

OBJECTIVE:To discuss the significance of prescription analysis in Chinese drugs dispensing and to probe into the profound connotation of the dispensing of prescriptions of traditional Chinese medicines.METHODS:Take the basic theory of Chinese medicine as the basis,the importance of prescription analysis in the dispensing of traditional Chinese medicines were analyzed from aspects of the patients' clinical diagnosis,medication intention as well as the function and properties of drugs etc.RESULTS:8 essential factors were found to be involved in prescription analysis,which shed new light on the omnidirectional pharmacy care provided for patients.CONCLUSION:The key for Chinese drugs dispensing lies in the prescription analysis.

Objective To compare the pharmacodynamic actions of Liuwei Dihuang Pills(LDP)prepared by four kinds of techniques on hemolysin contents in mice.Methods Sensitized by SRBC,immuno-inhibited mice models were induced by dexamethasone and cyclophosphane respectively.The hemolysin contents in the mice fed with LDP prepared by four kinds of techniques were observed to compare the pharmacodynamic actions.Results In experiment 1 that immuno-inhibited mice models were induced by cyclophosphane,the half hemolysin concentration(HC50)was low in the model group and LDP groups,especially in the LDP groups of techniqueⅡ,Ⅲ and Ⅳ.In experiment 2 that immuno-inhibited mice models were induced by dexamethasone,the difference of HC50 was insignificant between the blank control group,LDP groups of techniqueⅠand concentrated LDP group,the difference also being not significant in LDP groups of techniqueⅠat different dosages.Conclusion The dose of LDP can be reduced according to the experiment,however,the process technique of LDP needs to be improved.For this prescription is indicated for the treatment of yin-deficiency syndrome,dexamethasone can be used as the proposed drug for the establishment of the immuno-inhibited models.To confirm the reasonability of formula revolution,more parameters should be added to observe the pharmacodynamic actions.

Objective In order to explore the effectiveness of defluoride project on the control of endemic fluorosis in Yantai economic & technological development area. Methods Nine hundred forty-five children aged 7-12 were selected in 4 endemic fluorosis disease areas (Guxian,Bajiao,Dajijia Streets and Changjiang community) and the control area of Yantai economic & technological development area. Prevalence of children's dental fluorosis was determined by Dean method. Water fluoride content was measured in the 4 endemic fluomsis disease area by means of fluorides ion selective electrode. Results Childr dental fluorosis prevalence rate in Guxian,Bajiao,Dajijia Streets and Changjiang community was respectively 30.1% (55/183),41.2% (73/177),33.7% (66/196),31.8% (64/201),higher than those in the control area [13.3% (25/188)]; the median of water fluoride in the 4 disease areas was 1.84,3.46,2.39,2.10 mg/L,respectively,higher than the National Health on the control of endemic fluorosis is not obvious,Standard (1.00 mg/L). Conclusions Effect of the defluoride project in Yantai economic & technological development area needs to be improved.

RNA interference(RNAi) is an high efficient,specific gene silencing.The discovery process of RNAi and its mechanism was reviewed.In the further,RNAi will play important roles in characterizing new genes in the metabolic pathways,improving plant nutritional value,enhancing plant resistance and creating new varieties.

Objective To evaluate the role of mierosurgery in surgical treatment to urethrocutaneous fistula after urethroplasty in hypospadias and improve surgical results.Methods From 1999 to 2006,44 urethrocutaneous fistulae (more than 3mm in diameter) after urethroplasty for hypospadias in 33 patients were repaired with different skin flaps.For example,Thiersch technique,urthroplasty,etc.Microsurgical tech- nique was employed in every case.Results The success rates of different procedure were 84.8% (28/33) for Thiersch technique,100% (11/11) for urethroplasty respectively.The total success rate was 88.6%(39/ 44).Conclusion It's just application of skin flap for repairing of big or complex urinary fistula after hypos- padias surgery.The application of microsurgical technique can increase success rate.It is necessary to excise scar and partial urethra for hypospadias fistula combined with urethral structures,cicatricial eontracture and in- curvation of penis.Rich blood-supply,low tension and atraumatic technique are all very important to improve surgery success rates of urinary,fistula after hypospadias repair.

Objective To investigate the nursing experience during posterior cruciate ligament(PCL)reconstruction using LRAS artificial ligament under arthroscopy.Method The experience in nursing 51 cases of PCL reconstruction using LARS artificial ligament under arthroscopy from 2009 to 2012 was retrospectively summarized.Results It showed that KDC score of the 39 cases was excellent and 12 cases good,with the excellent and good rate of 100.0%.The average operation duration was(70.0±6.0)min and the average blood lose(50±3.6)ml.Conclusion Sufficient preparation before operation,much familiarity with LARS artificial ligament,skilled and accurate surgical cooperation are important factors for the success of PCL reconstruction using LARS artificial ligament under arthroscopy.

BACKGROUND:Eucommia ulmoides gum has good thermoplastics, liquidity, bondability and antimicrobial properties. However, the application of Eucommia ulmoides gum as a root canal filing material is rarely reported. OBJECTIVE: To compare the different contents of gas phase nano-silica on mechanical performance of composite material taking the gutta-percha as the matrix, and observe the effect of the root canal filing. METHODS:Four kind of root canal filing materials were prepared through adding barium sulfate (20%), nano-hydroxyapatite (10%) and different contents of gutta-percha and gas phase nano-silica (40% and 15%, 45% and 20%, 50% and 25%, 55% and 30%). PureEucommia ulmoidesgum served as the blank control. The mechanical properties (hardness, tensile strength and rupture elongation rate) of those five materials were detected. Furthermore, four kinds of composite materials, pureEucommia ulmoidesgum and Gutta-Percha Pelets were filed into the fresh extracted premolar root canal by using Obtura II technology. The effect of root canal filing was observed under X-ray and scanning electron microscopy.RESULTS AND CONCLUSION: Along with the increase of the content of gas phase nano-silica, the hardness of Eucommia ulmoides gum composite material increased, tensile strength increased firstly and then decreased, and rupture elongation rate decreased. PureEucommia ulmoides gum was transmitted to the X-ray and no image of filing material was obtained. Four kinds of composite materials were resistant to X-ray, and images showed that al composite materials were equaly filed into the root canal, without cacuole, and were in contact with the root canal inner wal, showing good filing effect. Scanning electron microscopy showed that, fingerlike projections were the longest at the surface of pureEucommia ulmoides gum; as the content of gas phase nano-silica increased, fingerlike projection length reduced at the surface of composite materials.Eucommia ulmoides gum composite materials could be developed as a canal filing material under heat flow condition.

Objective A method was developed for the determination of cyanide in water by ion chromatography with direct amperometric detection.Methods The eluent used was 0.1 mol/L NaOH and 10%(V/V) acetone.The samples were analyzed by using ion chromatography as well as external standard curve.The effects of 7 common ions,2 electrochemical active substances on the determination of cyanide were studied.Results The linear range of the method was 0.05-100 ?g/L and the correlation coefficient was 0.999 1.The RSD was

The paper introduced the concept of project management and its basic methodology. By analysis of existing problems found in international cooperation projects of hospitals, the authors probed into the feasibility of leveraging advanced project management methods in such projects, and came up with improvement measures. Such measures proposed include building a new structure for project management, improving project management system and operation mechanism, building supportive services and support systems, and special project culture as well.