We determined the polysaccharide content of different grade Xinjiang Lycium Barbarum L. in the way of colo]rimetry of phenolsulfuric acid at 490nm wavelength. All of them contained polysaccharide, But the higher content was found to be in big, red and thick fruits.

BACKGROUND:The incidence rate of cervical abnormalities of the patients with skeletal class Ⅱ and normal occlusion has been studied abroad, but the researches on the incidence of cervical abnormalities in different malocclusion patients is rare at home. OBJECTIVE:To observe the imaging of patients with cervical abnormalities and to statistics the incidence of cervical abnormalities in different malocclusion patients through analyzing the lateral cephalogram of different malocclusion patients. METHODS:Skeletal class Ⅱ group was consisted of 93 patients, 41 male (aged 18-40 years) and 52 female (aged 22-35 years), with the A point-nasion-B point angle>5°. Class Ⅰ group was consisted of 45 patients, 31 female (aged 20-36 years) and 14 male (aged 17-38 years), with the 1°

Objective To study the relationship among hydrops fetalis(over 28 gestational weeks),Mirror syndrome and preeclampsia(PE). Methods Clinical data of 13 cases of hydrops fetalis were analyzed and relative publications were reviewed. Results Eight out of the 13 cases were diagnosed as PE,and 5 were Mirror syndrome.Serious maternal complications occurred in all cases with either Mirror syndrome or PE. ConclusionsFetaland placental hydrops was closely related with maternal symptoms.Clinicians should be aware of this condition and detect Mirror syndrome in time so as to prevent its progress to PE.However,the pathogenic mechanism remains to be fully elucidated.More attention should be paid to both mothers and the hydrops fetalis.

Objective:To investigate the relationship between changes of the concentration of IL -12,IL-4,IFN-γwithin cervical local immune microenvironment and cervix infected with HPV 16,58,to understand the prevalence of physical status of HPV 16/58 DNA into the host genome in cervical lesions of different grades and to detect the relation between the integrated state of virus and the progress of cervical lesions.Methods: Detecting 89 cases of cervicovaginal lavage fluid and 140 cytological specimens of the cervix which from patients having had HPV-DNA genotyping assays and coming from the Affiliated Tumor Hospital of Guangxi Medical University from 2012.8 to 2013.5.89 cases of cervicovaginal lavage fluid were divided into 58 cases of HPV16 (+) and 31 cases of HPV58(+).Cytokines IL-12, IL-4, IFN-γin the cervical microenvironment were detected by ELISA assay.140 cases of cervical exfoliated cells were divided into 106 cases of HPV16(+) and 34 cases of HPV58(+).Using qRT-PCR to quantify the copy number of E2 and E6 genes and analyze the physical status of HPV 16/58 DNA according to E2/E6.Results:①The level of IL-4 in HPV16(+) was significantly higher than that in HPV58(+)(P<0.05).The levels of IL-12 and IFN-γwere lower than that in HPV58(+)(P<0.05).②Significant differences of DNA state were found between the groups of HPV 16(+) and HPV58(+)(P<0.05)and the rate of HPV16 DNA integration was significantly larger than that of HPV 58 DNA.Conclusion:The infection of HPV16 and HPV58 not only can lead to reduction of IL-12,IFN-γand increasing of IL-4 in cervical local immune microenvironment but also lead to viral integration in cervical cells,resulting in cervical lesions and cervical cancer through the internal and external changes in cervical cells .HPV16 is more likely to cause cervical cancer tissue than 58.

Objective To explore the reliability, advantages and precautions of trans-umbilical single site double-ports laparoscopy hernia sac high ligation in children. Methods Retrospective analysis of the clinical data of 102 patients of trans-umbilical single site double-ports laparoscopy hernia sac high ligation from April 2015 to June 2016, then summarize the treatment process, method and experience. Results All 102 cases of children with inguinal hernia, 84 cases of unilateral, bilateral in 18 cases. Unilateral cases, found in 24 cases of lateral occult hernia. Operation time: one side (16.17 ± 8.23) min, bilateral (23.68 ± 9.72) min. All the patients were discharged after 1 d, and the incision was beautiful. There was no swelling in the scrotum or labia, no hematoma, no adhesive intestinal obstruction, and followed up from 6~20 months without recurrence. Conclusion It is safe, reliable and effective to treat inguinal hernia in children with trans-umbilical single site double-ports laparoscopy hernia sac high ligation. It has advantages such as beautiful incision, small trauma, quick recovery, low requirements on technology and equipment, easy to grasp.

Objective Clinical research was made to observe the relationship between idiopathic thrombocytopenic purpura(ITP)and Helicobacter pylori(Hp)and to evaluate the effect of Hp eradication in the treatment of refractory ITP.Methods 13C urea breath test and Hp antibody of serum were made in 48 previously untreated patients with ITP from Department of Hematology of the First Affiliated Hospital of China Medical University during November 2004 to May 2005 years and normal controls consisted of 52 heathy peoples whose gastroscopy was normal.Omeprazole,clarithromycin and amoxicillin were used for Hp eradication to refractory ITP with Hp infection.13C urea breath test,Hp antibody of serum and the level platelet and PAIgG was detected after therapy 4~8 weeks.Results Of 48 cases with ITP,33(68.18%)had Hp infection and 24 of 52(46.12%)normal population had Hp infection,the infection rate of Hp in ITP patients being statistically higher than the control group(P

Accidents, Occupational ; prevention & control ; statistics & numerical data ; Humans ; Poisoning ; epidemiology ; prevention & control

Objective Study the effect of chlorogenie acids(CHA)on insulin resistance in obese rats induced by high‐fat diet . Methods We induced the obese rat model by feeding high‐fat diet ,obese rat model were divided into 4 groups:model group ,piogli‐tazone group(4 .5 mg/kg) ,CHA large dose group and group ,and finally determinated the levels of glucose tolerance ,serum insulin , serum lipid profiles and others .Results CHA showed a higher anti‐obesity activity with lower rate of increase of obese rats′body weights ,reversingglucose intolerance induced by high‐fat diet ,ameliorating the hyperinsulinemia ,decreaseing the levels of TG and TC ,and increase liver glycogen and muscle glycogen level compared with other group which treated with high‐fat diet .And in‐creased HOMA‐ISI ,decreased HOMA‐IR .Conclusion CHA can ameliorate the symptoms of insulin resistance in obese rats ,which mechanism may be related with CHA can stimulate glucose uptake and utilization by peripheral tissues ,and decrease the the serum levels of FFA ,decrease oxygen stress ,prevent and cure the injury induced by lipid peroxidation .

Transdermal drug delivery is an administration route which can avoid the first-pass effect,maintain steady plasma concentrations and enhance bioavailability. Drug transporting through the skin by passage through the stratum corneum leads to the viable epidermis and the dermis.With the development of the computer technology,many mathematical models for predic-ting the absorption of drugs have been built according to physical and chemical properties of drugs and physiological characteristics of each skin layer.This article presented provides a summary of the progress of mathematical models for predicting percutaneous absorption of drugs.

In this paper the investigation of using a new method-ABC-ELISA in assay of Autoimmune Thyroid Disease are presented. The sensitivity of ABC-ELISA is compared with that of standard ELISA; Its reliability is proven by the methods of detecting TSAb with FRTL-5. TRAb is detected by ABC-ELISA in 91% of untreated Graves'. TRAb is detected by Standard ELISA in 70% of untreated Graves'. The results of ABC-ELISA in 26 untreated Graves' are equal to that of the method of detecting TSAb with FRTL-5. Therefore, we consider that ABC-ELISA is a sensitive, reproducible, convenient method applicable to clinical practice.