Objective To explore the interference situation of several diseases in the detection of treponema pallidum antibody by chemiluminescence method.Methods One hundred and ten samples of 9 kinds of different infectious diseases and 200 clinical random samples were collected.Three hundreds and ten samples were simultaneously detected by using the Abbott ARCHITECT i2000 automatic chemiluminescence immunoassay analyzer and matched reagents,and the Roche E602 automatic chemiluminescence instrument and its matching reagent.The samples of inconsistent results were confirmed by the gelatin particle agglutination test (TPPA).Results There were 5 samples with inconsistent results detected by two instruments,5 samples confirmed by TPPA were negative,and 4 cases were the samples of multiple myelome.Conclusion (1)Abbott ARCHITECT i2000 and Roche E602 have a good consistency in detecting treponema pallidum antibody;(2)for the patients of multiple myeloma,the interference may exist in detecting treponema pallidum antibody by chemiluminescence method,which may cause false positive results and needs to be vigilant in our daily work.

Purpose:To study the malignant probability of a solitary thyroid nodule,probable risk factor,the status of B-mode ultrasonography,computed tomography,fine needle aspiration and frozen section in the operation and other adjuvant examination in the diagnosis.Methods:In the retrospective analysis of 902 cases of solitary thyroid nodule treated by surger- y and diagnosed by pathology in the Cancer Hospital of Fudan University from March 1998 to May 2001,we analyzed the value of B-mode ultrasonography,computed tomography,fine needle aspiration and frozen section in the operation and com- pared them with the final pathological conclusion.Results:This disease occurred mostly in 20～50 years old women,while the malignant nodules occurred more frequently in men(P50 years) (P50 years) is a risk factor.B-mode ultrasonography can be used as routine preoperative examination.CT is valuable in the cases with metastasis to neck lymph nodules.With FNA the pathological diagnosis of the nodule suspected to be malignant can be made preoperatively.

Acupuncture Points ; Acupuncture Therapy ; Adolescent ; Adult ; Aged ; Female ; Humans ; Male ; Middle Aged ; Migraine Disorders ; physiopathology ; therapy ; Occipital Bone ; physiopathology ; Young Adult

Objective To examine expression of PTEN gene in ovarian cancer cisplatin-sensitive cell line OV2008 cells and cisplatin-resistant cell line C13K cells,and evaluate the effect of wild-type PTEN gene on reversing cisplatin-resistance of C13K cells and underlying mechanisms.Methods The expression of PTEN mRNA and protein in OV2008 and C13K cells were detected by semi-quantitative RT-PCR and western blot.Recombinant eukaryotic expression plasmid containing human wild-type PTEN gene was transfected into C13K cells by lipofectamine 2000.The expression of PTEN mRNA was monitored by RT- PCR and the expression of PTEN,protein kinase B(AKT),phospho-AKT(p-AKT)protein were analyzed by western blot in PTEN transfected and untransfected C13K cells.Proliferation and chemosensitivity of cells to cisplatin were measured by methyl thiazolyl tetrazolium(MTT),and cell apoptosis was detected by flow cytometry after treatment with cisplatin.Results(1)The expression of PTEN mRNA and protein(1.02 ?0.05,1.02?0.07)in OV2008 cells were significantly higher than those in C13K cells,which were 0.45 ?0.03 and 0.55?0.03 respectively(P

Adolescent ; Female ; Humans ; Jaw ; pathology ; Parathyroid Neoplasms ; complications ; pathology

Adult ; Aneurysm, Dissecting ; complications ; diagnosis ; Aortic Aneurysm ; complications ; diagnosis ; Diagnostic Errors ; Female ; Humans ; Myocardial Infarction ; complications ; diagnosis

Adult ; Facial Hemiatrophy ; Humans ; Male

Adult ; Blood Glucose ; metabolism ; Female ; Herbicides ; poisoning ; Humans ; Liver ; physiopathology ; Male ; Middle Aged ; Paraquat ; poisoning

Aged ; Humans ; Male ; Nasal Septum ; pathology ; Nose Neoplasms ; Odontoma

Acupuncture Points ; Acupuncture Therapy ; Adolescent ; Adult ; Child ; Female ; Hordeolum ; therapy ; Humans ; Male ; Middle Aged ; Young Adult