OBJECTIVE:To probe into the standardized administration in the public bid for drug purchase of medical institutions.METHODS:Experiences and understandings in carrying out ISO9001standards in the public bid for drug purchase of our hospital were introduced;problems involved in which were analyzed and some advices were put forward.RESULTS&CONCLUSION:The practice of ISO9001standards does good to the consummation and standardization of the public bid of drug purchase in Chinese medical institutions.

OBJECTIVE:To study the general layout on the quality control of clinical pharmacy work of Xuzhou city during the period of2005～2008.METHODS:The problems appeared in hospital clinical pharmacy were analyzed by adopting ISO9001process control theory,i.e.PDAC cyclical quality management method,and suggestions on the corresponding scheme and practices were raised.RESULTS&CONCLUSIONS:Studying the general layout on the quality control of clinical pharmacy work by means of ISO9001process control theory is conducive to the development of clinical pharmacy of Xuzhou city.

Objective To assess the impact of parents' occupational class and intergenerational occupational mobility on children's mental health so as to provide evidence for improving health equity.Methods Chinese general social survey 2005 (CGSS2005) dataset was used for multivariate analysis of variance (MANOVA).Results The proportion of children whose father or mother was of lowerclass occupation was 59.0％ and 62.9％,respectively.When grew up,this figure dropped to 49.8％.Those whose father was in the higher-class occupational group reported better mental health status (F =1.449,P=0.015).Downward occupational class mobility experience also increased the risk of mental illness (F =3.050,P =0.048).Conclusion This study suggests an independent relationship between mental health and parents' occupational class and intergenerational occupational mobility.

Blood Glucose ; analysis ; Diabetes Complications ; prevention & control ; Diabetes Mellitus, Type 2 ; blood ; therapy ; Humans ; Risk Factors

Objective To study the correlation between the serum high mobility group protein B1 (HMGB1)and the acute organophosphorus pesticide poisoning(AOPP). Methods The serum HMGB1 levels of the 116 patients with AOPP(AOPP group)and 40 healthy adults(control group)were detected by immunoblotting method. According to illness severity, AOPP group was divided into mild group(40 cases),moderate group(39 cases)and severe group(37 cases), and severe group was divided into multiorgan dysfunction syndrome(MODS)group(20 cases)and no MODS group(17 cases). The serum levels of CHE,HMGB1 were compared. Results The absorbance of HMGB1 in severe group(2.91±0.12)was significantly higher than that in moderate group(2.15±0.17), mild group(1.16 ± 0.29)and control group (0.84±0.30)(P＜0.01).The absorbance of HMGB1 in moderate group was significantly higher than that in mild group and control group(P＜0.01). The absorbance of HMGB1 in mild group was significantly higher than that in control group(P＜0.05). The absorbance of HMGB1 in MODS group was significantly higher than that in no MODS group(P＜0.01),but the absorbance of CHE had no significant difference between two groups(P＞0.05). Conclusions The degree of AOPP has notable correlation with the level of serum HMGB1. The level of serum HMGB1 is an useful index for evaluating the degree of AOPP.

Objective] To study the Xin'an doctor Sun Yikui Sun Wenyuan basis four diarrhea cases, in order to reveal Sun Yikui diarrhea syndrome differentiation syndromes characteristics. [Method] With Sun Wenyuan basis of diarrhea four proven cases as example, the combination of Neijing, Treatise on Febrile Disease, the Synopsis, Mingmen theory, Danxi academic ideas and related works and academic thoughts of Sun's, interpretation of Sun's diarrhea cases, comprehensive analysis of the syndrome differentiation syndromes characteristics. [Results] From the Sun Wenyuan basis diarrhea in four cases to know Sun Yikui grasp of vital theories and Danxi academic thoughts, clinical cure, the doer phlegmy wet, dyspepsia, the accountability of virtual are of pixu(deficient spleen), kidney deficiency, with particular emphasis on the pathological factors of kidney Yang deficiency, the author was good at using insurance and pills, Erchen Decoction, Benefit-stomach Shengyang Moisture-penetration soup, Liujunzi Decoction and revised prescriptions. [Conclusion] Sun Wenyuan basis contained diarrhea basis, Sun's clinically curing diarrhea with outstanding characteristics, the curative effect is distinct, we should combine classical and Sun's works to conduct the thorough research to the related basis, broaden the clinical cure drainage, help to improve the ability of clinical treating diarrhea.

Objective:To obesrve the influence of DNA-PKcs in the chemoresistance of multi-drug resistance malignant glioma cells,and to explore its molecuIar mechanism in chemoresistance.Methods:siRNA was used to construct the DNA-PKcs knockdown human glioma U251 cell line;Western blotting method was used to detect the expressions of DNA-PKcs in U251 cells (U251 cells), doxorubicin (ADM)resistant U251 cells (U251/ADM cells),DNA-PKcs knockdown and ADM resistant U251 cells (U251/ADM/siDNA-PKcs cells).CCK8 method was used to detect the cell proliferation activity in three groups;Western blotting method was used to detect the expressions of MDR1, pNF-κB/p6, total Akt, pAkt/T308 and pAKT/S473 in the cells in three groups. Results:The expression level of DNA-PKcs in U251/ADM cells was significantly higher than those in U251 cells and U251/ADM/siDNA-PKcs cells (P < 0.01 ).The IC50 values of doxorubicin (ADM),paclitaxel (PTX), gemcitabine (GEM)in U251/ADM/siDNA-PKcs cells were significantly lower than that in U251/ADM cells (P <0.05);the expression levels of pAKT/S473,pNF-κB/p65,and MDR1 in U251/ADM/siDNA-PKcs cells were significantly lower than those in U251/ADM cells (P <0.01),but the total Akt and pAkt/T308 had no significant differences between two groups (P >0.05).Conclusion:DNA-PKcs can significantly enhance the chemoresistance of multi-drug resistance malignant glioma cells,the underlying mechanism is related to up-regulation of pAKT/S473,pNF-κB/p65 and MDR1 expressions.

To prepare and study the pharmacokinetics and release bioavailability in olunteers and concentrations in plasma in patients. METHODS: Ethylcellulose was used matrix in phase separation-coacervation for preparation of microencapsulation. The release experiments were performed in a rotating shaker. The isoniazid concentration in plasma was determined by spectrophotometrical method following a single oral dose of sustained-release cupsule and ordinary tablet respectively given to 10 volunteers in a open randomized cross-over test. MCP86 was used to process main pharmacokinetic parameters. RESULTS: The sustained-release of capsule and ordinary teblet in vitro, T50 was 1 h and 0.032 h respectively. The drug in sustained-release capsule was sustained release over 10 h. The main parameters in body: ordinary tablets: cmax=11.12 μgml-1, tmax=1.41 h, K=0.201 h-1; sustained release capsule: cmax=4.99 μgml-1, tmax=1.80 h, K=0.03 h-1. The concentration of blood at 36 h was (0±0)μgml-1 and 1.63 μgml-1 respectively. Except tmax, there was significant difference between the two fomulations (P＜0.01). The concentration of blood in patient at 1.5 h and 36 h. ordinary tablet and sustained-release capsule respectively were (8.24±2.60)μgml-1, (0±0)μgml-1and (3.69±0.86)μgml-1, (2.09±0.56)μgml-1. CONCLUSION: The sustained-release capsule will play an important part in prevention and treatment of tuberculosis as the result of its reasonable formulation and simple technology.

AIM: To establish the technique of precision-cut fibrotic liver slice (PCLS) and grope the optimal cultural conditions for researching the liver xenobiotic metabolism in vitro and the drug interaction. METHODS: Complex factors (higher fat diet, alcohol and CCl 4) were used to make the animal model of liver fibrosis. Fibrotic liver slices were prepared and cultivation system was established. Lactate dehydrogenase (LDH) leakage, glutathione S-transferase (GST) activity and 3[4,5-Dimethythiazole-2-yl]-2,5- diphenyltetrazolium bromide (MTT) reduction were chosen as indexes to assess the viability of the slice in different thickness, medium pH and cultural time. RESULTS: Rats were in earlier hepatic fibrosis after administration for 3 weeks. When the thickness of slices was 300 ?m and medium pH was 7.0, the LDH leakage, GST activity and MTT reduction could maintain on a steady level in 6 h. CONCLUSION: A 300 ?m of thickness, 7.0 of medium pH and 6 h of cultural time are the optimal slicing and culturing conditions for fibrotic liver slice.

Objective:To investigate the consistency of two methods in the measurement of bone mineral density in the lateral lumbar vertebra.Methods:The bone mineral densities of the 2nd,3rd and 4th lumbar vertebras in 18 female patients,at a preset side lying position,were measured respectively by two methods(lateral spine analysis,LS and lateral vertebral analysis,LVA) provided by the dual-energy(X-ray) absorptiometry(GE Lunar,Prodigy).The results were recorded and analyzed by SPSS(Version(10.0).) The values of bone mineral density were displayed by boxplot,paired and analyzed by(Wilcoxon's) signed rank test and(Pearson's) correlation analysis.Statistical significance was considered when P(0.05),) and were clearly correlated(P