Objective To investigate the condition of self-management of patient controlled analgesia pump in patients in the ICU.Methods 82 patients in ICU were selected in July 2014 and were randomly divided into the observation group and the control group with 41 cases in each.In the observation group,the PCAP was fixed on the bedrails of patients' bed and controlled by themselves to acquire satisfactory analgesia effect.In the control group,the PCAP was operated by the nurses.Pains scores was compared between the two groups.Results The different position of PCAP showed the different scores of pain in the patients.The pain scores in the observation group (2.93±1.47) was significantly lower than that in the control group (3.76±1.36),P＜0.05.Conclusions Proper position of PCAP and controlled by patients can effectively reduce postoperative pain of patients in the ICU,and then reduce their suffering and improve their comfort.

Hepatitis A is one of the global public health problems,and China has high prevalence. In order to decrease the incidence of hepatitis A,China has integrated Hepatitis A vaccine into Expanded Program on Immunization (EPI) in 2007. The paper will provide some ideas on the immunogenicity and impact factors of the different types of hepatitis A vaccines that are used in China.

Atrial Fibrillation ; pathology ; Humans ; Peptidyl-Dipeptidase A

Objective To find a new method to prepare the acellular matrix of the penile cavernous body. Methods Intact specimens of the penile cavernous body were collected from healty adult rabbits,treated with Triton-X100 and NH3?H2O,and then stained with HE.Light microscopy was employed to observe the acellular cells.Results Acellular matrix of the penile cavernous body was obtained after a 25-day treatment with Triton-X100 and NH3?H2O.The matrix was intact.HE staining showed that the matrix was composed of regularly arranged elastic fibers with wide intra-spaces without any cells inside.Conclusion Acellular matrix of the penile cavernous body can be prepared by using Triton-X100 and NH3?H2O.

ObjectiveTo summarize the recent therapeutic effects of mesh-plug tension-free hernioplasty in elderly inguinal hernia. MethodsAll cases were performed with mesh-plug case-hardened products, which are manufactured by American Bard Company. ResultsAll the operative procedures were performed smoothly. Postoperative complications were:6 cases of urinary retention,4 scrotal hydroceles,2 persistent wound pain,2 local lightly projections with foreign body sensation,2 hypoincisional haematoma. 1～24 months of follow-up were taken,2 recent recurrences were reported. ConclusionMesh-plug tension-free hernioplasty is a perfect surgical operation. Its main characteristics are:simple performance, less trauma, tension-free, time-saving, good recent therapeutic effect, and fewer recent recurrence. It is especially suitable to the elderly patients and/or Patients with other diseases.

Objective To express and purify the killer immunoglobulin-like receptor KIR3DL1 extracelluar domain.Methods pUC57-KIR3DL1 was used as template,KIR3DL1 extracelluar domain was amplified by polymerase chain reaction(PCR)and cloned into pGEM-T vector with A-T cloning technique.After DNA sequence analysis,the target fragment was inserted into the prokaryotic expression vector pET28a-DsbA to construct the recombinant vector pET28a-DsbA /KIR3DL1.The recombinant plasmid was transformed into E.coli BL21(DE3),and induced with IPTG.Bacterial pellets were resuspended in 8M urea and centrifuged to remove the insoluble material.The crude extract was purified by passing over a Ni-NTA-agarose column.After the inclusion body flowing through the Ni-NTA-agarose affinity chromatography was refolded successfully,it was purified by Superdex75 gel filtration and the purification effects of the fusion protein were identified by SDS-PAGE and western blot.Result Some fusion proteins were expressed in the supernatant,and the others were expressed in the form of inclusion bodies.The purity of fusion protein was over 95% after purification under denaturing condition.Conclusion The highly efficient expression of KIR3DL1 extracelluar domain laid the foundation for the further studies on exploration of the mechanism of immunization recognition between KIR3DL1 and its ligand.

Retrospective study was carried out in 93 cases of endometrial carcinoma admitted to our hospital since Jan. 1985 to Dec. 1994. Thirty five cases were older than 60 years, and 24 cases were younger than 50 years. All of the cases had pathological diagnosis and were staged according to FIGO. When compared with the younger group, the rate of older patients with stage Ⅰa was higher (11.4% in younger and 45.4% in older, P

The expression of ZNF191 mRNA in 31 ovarian malignant tumors and 31 normal ovary tissues were investigated by reverse transcription-polymerase chain reaction (RT-PCR). It was found that expression level of ZNF191 mRNA in malignant ovarian tumors was much less than that in normal ovary tissues (P

Objective To observe the interference actien of Buqi Huoxue Decoction(Herbal decoction for reinforcing qi and activating blood)on the model rats with acute cerebral ischemia.Methods Sixty SD rats were randomized into 6 groups,i.e.sham operation group,model group,nimodipine group,and Buqi Huoxue Decoction groups of high,middle,and low dosages,with 10 rats in each.Except the model and sham operation groups,the others were given Buqi Huoxue Decoction through intragastric gavage for 5 days.The acute cerebral ischemia models were made on the sixth day.One hour before modeling,the last medication was applied.At the time point 6h,12h,and 24h,the blood sample was taken from the aorta abdominalis for detecting the tumor necrosis factor-?(TNF-?).After 24h,the hemorheology test was performed.Results Compared with the model group,the whole blood viscosity including the high shear rate and low shear rate and erythrocyte aggregation index of the Nimodipine group and Buqi Huoxue Decoction groups of high and middle dosages were lowered(P

Objective To assess the efficiency of algae extermination by the combined copper electrolysis technology. Methods The combined copper electrolysis technology and copper sulphate were used to treat the Synechocystissp strain PCC6803 in the steady stage of culture in different time and the efficiency of algae extermination was assessed. Results No alga in the water sample was detected after 0.5 and 1 hour of treatment with copper electrolysis technology and copper sulphate respectivly as the concentration of Cu2+ was at 0.3 mg/L. The extermination rate of algae was 80.75% after 12 days of treatment of combined copper electrolysis. This technology could effectively decrease the turbidity, smell, COD, ammonia nitrogen and increase the dissolved oxygen in the treated water. Conclusion The Cu2+ released by copper electrolyzing has a stronger efficiency of algae extermination compared with copper sulfate. The combined copper electrolysis technology will be a satisfactory method for algae extermination in the landscape water.