Objective To investigate expression and significance of matrix metalloproteinase-9 (MMP-9) and neutrophil gelatinase-associated lipocalin (NGAL) in serum and placental tissue of preeclampsia maternal.Methods 74 cases of preeclampsia pregnant women as research subjects,which 39 cases of severe preeclampsia,35 cases of mild preeclampsia,selected 30 cases of normal late maternal as the control group.Three groups of maternal serum levels of MMP-9 and of NGAL were detected by enzyme-linked immunosorbent assay.RT-PCR was used to test placenta MMP-9 mRNA,and NGAL mRNA level.Results Preeclampsia maternal serum levels of MMP-9 and NGAL level were significantly higher than those of severe preeclampsia pregnant women serum level of MMP-9 and NGAL were significantly higher than mild preeclampsia (t =3.127,2.846,3.508,3.284,all P ＜ 0.05).Preeclampsia placentas of MMP-9 mRNA and NGAL mRNA levels were significantly lower than the control group (t =-2.842,-2.728,-3.532,-3.276,(a)ll P ＜ 0.05),severe preeclampsia placentas of MMP-9 and NGAL were significantly higher than mild preeclampsia(t =2.732,2.741,all P ＜ 0.05),severe preeclampsia placentas of MMP-9 mRNA and NGAL mRNA levels were significantly lower than in patients with mild preeclampsia (t =-2.681,-2.587,all P ＜ 0.05).Preeclampsia maternal serum levels of MMP-9 and NGAL was positively correlated with placental tissue mRNA levels of MMP-9 and NGAL mRNA level was positively correlated (P ＜ 0.05).Conclusion Preeclampsia maternal serum MMP-9 and NGAL were significantly elevated,placental MMP-9 mRNA and NGAL mRNA levels were significantly decreased,suggested that they may be involved in the pathogenesis of preeclampsia.

Rapid and accurate diagnosis of pathogens is the responsibility of the clinical microbiology lab.Effective implementation of this function requires standard procedure of specimen collection and processing.In order to improve the ability of infectious disease diagnosis,clinical microbiology laboratory should strengthen the communication with the clinicians,to improve the quality and quantity of specimens.Meanwhile,microbiology laboratory should learn some clinical knowledge and understand the demand and development of clinical practice.Clinical microbiology lab should emphasize patient-oriented and multidisciplinary close cooperation,for improvement of pathogen diagnosis level.

OBJECTIVETo investigate necessity of the further processing for adsorptive resin material.

METHODSAccording to GB/ T 16886.4, coagulation tests, haematology tests, in vitro spontaneous platelet-adhesion test and complement activation tests were conducted respectively to assess the in vitro blood compatibility of adsorptive resin material.

RESULTSIn comparison with polypropylene, he adsorptive resin material appears to be a very slight activator to the endogenous blood coagulation system, it can inhibited the exogenous blood coagulation system very significantly (P < 0.01), it can adhere to fibrinogen, red blood cells, reticulocytes very significantly (P < 0.01), and platelet significantly (P < 0.05). The adsorptive resin material is also revealed to be a very significant activator (P < 0.01) to the complement system.

CONCLUSIONSThe in vitro blood compatibility assessment witnesses that it is very necessary for the adsorptive resin material to go through the certain special processing(such as the surface modification and the microencapsulation technology, etc) before its application to human being.

Magnetic mediated particles can be located within tumor volume through special ways are can be heated while exposed to an externally alternating magnetic field,thus kill the tumor cells.Compared with othen hyperthermia a major advantage of magnetic mediated hyperthermia is that of targeting tumor specially and selectively.The evidences of clinical research demonstrate that magnetic mediated hyperthermia will have a promising future and play an important role in tumor therapy.

Objective:To discuss the clinical effect of preoperative interventional chemotherapy and pathological changes in uterine cervical cancer.Methods:preoperative interventional chemotherapy by femoral intubatton were done in 21 patients with uterine cervical cancer.The project of chemotherapy was bleomycin 45mg and cisplatin or oxaliplatin 80mg/m2.Rusults:21 cases of uterine cervical cancer including 7 cases ofⅠstage and 14 cases of Ⅱstage received one or two times of preoperative interventional chemotherapy.All of the patients successfully completed cervical cancer radical therapy.All of the specimens were sent for pathological examination.A lot of lymphocyte cells infiltration and necrosis were found in the cancer tissues.Conclusion:preoperative interventional chemotherapy for uterine cervical cancer proves to reduce the local cancer size as well as decrease the bleeding of cancer.The patients can receive cervical cancer radical therapy after the interventional chemotherapy.

OBJECTIVE To understand infection status and drug resistance of Ureaplasma urealyticum(Uu) and Mycoplasma hominis(Mh) in female genitourinary tract in the local region.METHODS The results of mycoplasma cultivation and susceptibility tests of female vaginal secretions specimen in 409 cases during from Sep 2007 to Apr 2008 were analyzed retrospectively.RESULTS Among 409 cases,the positive rates were mycoplasma cultivation(57.2%).Uu(72.6%),Mh(3.4%) and combined infection(23.9%).Drug resistance of Uu to ten kinds of common antibiotics showed that minocycline

In this paper, some study methods of pharmacokinetic, the explanation ways of data and experimental methods of transdermal therapeutic system(TTS) were described. The advancement of modern science accelerates the development of pharmacokinetic of TTS and makes more progress in this field.

Objective:To discuss the diagnosis,treatment of uterus cornea pregnancy.Methods:Retrospective analysis was made in 21 cases of uterus cornua pregnancy is recent 10 years.Results:8 cases were diagnosed uterus cornua,9 cases were misdiagnosed ectopic pregnancy,1 case was misdiagnosed rupture of uterus of artifical obortion,1 case was misdiagnosed pregnancy with uterus septus,1 case was misdiagnosed choriocarcinoma.Surgical treatment was done in 19 patients,and the other 2 cases received conservative therapy.Conclusion:Cornual pregnancy is often misdiagnosed as ectopic pregnancy or other diseases.More careful gynecological and transobdominal ultrasound examination will improve the accuracy of diagnosis.

Objective To study the clinical value of procalcitonin on early diagnosis of sepsis .Methods 60 patients who entered ICU were enrolled in the present study were chosen .The change of procalcitonin , CRP, WBC,ESR,GRANR and the highest temperature was observed dynamically with 1 day and 4 days in hospitalization . The patients were ultimately divided into the two groups according to the diagnostic standard of sepsis ,the sepsis group (SIRS group) and infective systemic inflammatory response syndrome group (NONSIRS group).Results After ana-lyzing the processed data,researchers found that PCT of the sepsis group (6.73 ±1.31)ng/mL was higher evidently than that of the SIRS group(0.25 ±0.05)ng/mL(t=3.10,P<0.05),the area under ROC curve of PCT (0.869) is evidently greater than CRP(0.798),WBC(0.737) and ESR(0.394)(P<0.05),which had statistical significance. Conclusion Procalcitonin is a good index of early diagnosis on sepsis ,therefor,its sensitivity and specificity is higher than the other inflammatory indicators .Finally,procalcitonin can be widely used in clinical trial .