Endometrial Neoplasms ; classification ; epidemiology ; mortality ; pathology ; Female ; Humans ; Uterine Cervical Neoplasms ; classification ; epidemiology ; mortality ; pathology

Objective To investigate the effects of Rehmannia glutinosa polysaccha-rides (RPS) on myocardial apoptosis of rats after myocardial ischemia-reperfusion injury. Methods The experimental rats were randomly devided into sham operation group, model group, RPS low, medium and high-dose group, Danshen tablets group, 20 rats in each group. All the rats were performed the coronary occlusion operation except the sham operation group. And then, the rats in RPS low, medium and high-dose groups were given RPS 10, 20, 40 mg/kg by intragastric administration;the rats in Danshen tablets group were given Danshen tablets suspension 260 mg/kg;the rats in sham operation group and model group were given the equal volume of saline;once a day for 4 weeks. The areas of myocardial infarction were analysised by TTC staining;the apoptosis of cardiomyocytes was observed by TUNEL and the apoptosis index(AI) were calculated;the expression of bcl-2, Bax, caspase-3 were detected by IHC and were semi-quantitative analysised. Results Compared with the model group, the areas of myocardial infarction (31.7% ± 6.1%, 22.8% ± 5.5% vs. 43.9% ± 6.7%) and the apoptosis index (44.6%± 6.7%, 32.8%± 5.4%vs. 59.4%± 9.0%) in RPS medium and high-dose group (RPS 20, 40 mg/kg) significantly decreased (P<0.05 or P<0.01). The expression of bcl-2 (0.40 ± 0.09, 0.45 ± 0.08 vs. 0.26 ± 0.05) significantly increased (P<0.05 or P<0.01), the expression of Bax (0.55 ± 0.11, 0.28 ± 0.08 vs. 0.85 ± 0.13) and caspase-3 (0.11 ± 0.03, 0.08 ± 0.02 vs. 0.16 ± 0.03) significantly decreased (P<0.05 or P<0.01), and the ratio of bcl-2/Bax (0.73 ± 0.05, 1.61 ± 0.20 vs. 0.31 ± 0.08) significantly increased (P<0.05 or P<0.01). Conclusions RPS had inhibition effects on myocardial apoptosis after myocardial ischemia-reperfusion injury in rats, which may be related to its effects of enhancing the ratio of bcl-2/Bax and down regulating the expression of caspase-3.

Objective:To examine the effects of glucose on the expression of matrix metalloproteinases-2(MMP-2)and the changes of ultramicrostructure,cell cycle in cultured human umbilical vein endothelial cells.Methods:Human umbilical vein endothelial cells(hUVEC)were incubated in a culture medium with 11.2mmol/L,16.8mmol/L and 33.6mmol/L glucose concentrations for 24h,48h,72h respectively.The expression of MMP-2 was measured by RT-PCR and Western-blot.Results:When D-glucose concentrations rose,hUVEC exposed to high glucose concentration(11.2mmol/L,16.8mmol/L and 33.6mmol/L)increased in cell expression of MMP-2 after 48 h and 72 h of exposure,as compared with those cultured in medium with a low glucose concentration(5.6mmol/L).And cell cycle changed too.Conclusion:High concentration of glucose can arrest the proliferative response and show that even a short-term exposure of endothelial cells(ECs)to high glucose concentration leads to their activation associated with increased expression of MMP-2 and that this effect may appear more significantly along with time of exposure to high concentration of glucose.

Objective:To assess the clinical setting of a heavy silicone oil tamponade(Densiron-68) for rhegmatogenous retinal detachment(RRD).Methods:Twenty-nine eyes of 29 consecutive patients were recruited between December 2007 and February 2009.Primary vitrectomy with Densiron-68 was used in all cases.Patients to be selected had two groups:one group had 18 cases,mean age was 72.5 years.These patients were impossible prone because of ages and body factors.Among these of 11 cases had macular holes and posterior pole part holes,another of 7 cases had many holes.Another group had 11 cases,mean age was 45.8 years.These patients with inferior complex rhegmatogenous retinal detachment with secondary proliferative vitreoretinopathy(PVR) were included.Besides one case of giant retinal tear with between 2 and 7 clock hours,among these of 7 cases had one or two scleral buckling procedure,or pars plana vitrectomy with oil endotamponade.But inferior recurrent retinal re-detachment appeared.Surgical techniques included pars plana vitrectomy,membrane peeling,heavy water application,endophotocoagulation,cryocoagulation,endotamponade et al.Results:1or 13-month follow up,mean 4.3-month.Densiron-68 removal with 25 cases was after operation of 2 months,and didn't remove of 4 cases.After Densiron-68 removal,majority showed a stable reattached retina without further interventions,while,in one or two patients,recurrent retinal re-detachment appeared during Densiron-68 removal.Some of patients had partly lens opacification or lens post-capsular opacification.Part of patients had ocular hypertension.Conclusion:With Densiron-68,high anatomical and functional success rates can be achieved with inferior complex rhegmatogenous retinal detachment.Heavy silicone oil tamponade improves inferior tamponade,and may be considered a new generation of intraocular tamponades.But there were a significant early emulsification.Despite adequate Densiron fills,emulsification necessitated its removal.

Objective:To study the fluorescent manifestations of fundus oculi lesion in several types of retinal degeneration.Methods:Fundus fluorescein angiography(FFA) were performed in 8 patients(16eyes)of retinal degeneration.Results:Lesion of eyes was actinomorphic,and was relatively quiet,grade was low,mainly presented as retinal pigment epithelium(RPE) and choroidal capillary atrophy.Background image choroidal at early stage showed hypofluorescence,and big choroid blood vessel low fluorescence were seen.This differed from secondary retinitis pigmentosa.Conclusion:Results of FFA with retinal degeneration show barrier of choroidal capillary atrophy may be one factor for RPE and neurosensory retina dystrophy.

Objective To study the outcome of pregnancy with lupus nephritis. Methods The clinical data of 9 pregnancies with lupus nephritis were retrospectively malyzed. Results Hypertension,proteinuria,injured renal function and abnormal serological marker were complicated with nine patients with lupus nephritis in pregnancy. The outcome of pregnancy was as following:abortion 2 cases,stillbirth 1 case,premature delivery 4 cases,term delivery 2 cases. All neonate survived without malformation. There was no postpartum hemorrhage or death. Patients with active SLE had very high fetal mortality( 100% ). And SLE got worsen in 50% of them. However,fetal mortality rate in pa-tients with controlled or quiescent SLE was 11.1% ,which was much lower than it in other patients(P<0.05). Con-clusion Lupus nephritis should be controlled before pregnancy. Pregnancy outcome could be improved by careful multidisciplinary monitoring and proper usage of adrenal cortical hormone.

Natural killer (NK) cells have long been considered the only representative of lymphocyte lineages among the innate immune system ,but recent studies have revealed that several types of innate lymphoid cells ( ILC ) exist in both humans and mice.These newly identified ILC populations were mainly distributed at mucosal barriers ,regardless of their rarity ,they play important roles in the defense against pathogens and in the maintenance of tissue or organ homeostasis .In the early stages of ILC development ,a common ILC lineage-restricted progenitor exists and under the control of different transcription factors ,the progenitor can later give rise to different ILC subsets with distinct phenotypes and functions.Different ILC subsets exhibit distinct cytokine secretion profiles ,based on the categorization of helper T cell subsets , ILC family has been further classified into three groups.The finding of diverse ILC extremely enriches the content of innate immunity ,and also provides new insights into links between innate and adaptive immunity .

Objective To investigate the methods for the detection of serum samples with low-concentration HBsAg and its clin-ical value.Methods Serum samples with HBsAg weak positive were measured by ELISA or using Roch cobas e601 were collected from the clinical laboratory.The samples with low-concentration HBsAg were confirmed by neutralization assay by using Lizhu ELISA reagent Kit or Roch cobas e601 analyzer.at the same time,ALT/AST concentrations and HBV DNA copies were also deter-mined.Results Among 53 samples that showed weak positive reaction by using Kehua reagent kit,37 samples were positive in neu-tralization test,while the other 16 samples were negative.Among 18 samples that showed weak positive reaction tested by using Roch cobas e601 analyzer,13 samples showed positive in neutralization test,and the other 5 samples showed negative.Conclusion The results of HBsAg test varies in different detection System.The clinical significance of samples with low-concentration HBsAg needs further study.

A new NK cell subset with antigen presentation function was recently found and called as interferon-producing killer dendritic cells (IKDC cells).These cells co-express surface markers of NK cells and DCs;functionally,they can secrete interferons,kill target cells and have antigen-presenting ability.IKDCs extensively exist in almost all lymphoid tissue or organs with similar phenotype,but recent studies indicated that IKDCs were developmently not from DC cells but from NK cells,the conclusion of which is still in its early stage.The discovery of IKDC will be help to understand the link between innate and adaptive immunity.

Objective:To explore the relationship between coronary slow flow (CSF) and vascular inflammatory fac-tors .Methods :A total of 229 inpatients with normal coronary angiography (CAG) results were selected as research object .According to CAG TIMI flow and corrected TIMI frame count ,patients were divided into CSF group (n=102 ,no obvious lesion in epicardial coronary artery but coronary flow was slow ) and normal coronary group (n=127 ,completely normal epicardial coronary artery and blood flow ) .General clinical data were collected ,vascular inflammatory factors were measured and their relationship with CSF was analyzed .Results:Compared with normal coronary group ,there were significant rise in levels of high sensitive C reactive protein [ (1.41+0.72 ) mg/L vs . (2.82+0.58) mg/L] ,interleukin 1 [ (0.18 ± 0.05)μg/L vs .(0.26 ± 0.06)μg/L] and interleukin 6 [ (0.363 ± 0.016) ng/L vs .(0.465 ± 0.015) ng/L] in CSF group , P<0.05 all .Logistic regression analysis indicated that vas-cular inflammatory factors were risk factor for CSF occurrence (OR= 1.008 , P= 0.015 ) .Conclusion:Coronary flow velocity is closely related to vascular inflammatory factors .