ObjectiveTo observe the effectiveness of Responsar against Anopheles sinensis and Anopheles anthropophagus. MethodsThe cotton and nylon gauze absorbing Responsar solutions of 10,15 and 20 mg/m~2 (effective dosage) were used in the test. An. sinensis and An. anthropophagus raised in the lab were used as probational worms, having the knockdown power, lethiferous power and effective keeping observation. ResultsWith Responsar of 10,15 and 20 (mg/m~2) impregnating two different kinds of bednets, the KT_(50(s)) of An. sinensis and An. anthropophagus were between 3.98 and 7.30 min after touching the bednets. The knocked down mosquitoes were resumed breeding for 24 h, and the mortality was 100%. Touching nets 3 min, resuming breeding for 24 h, the mortality of mosquitoes was more than 90%. The nets hung for 180 d after impregnated with the insecticide and the knockdown power was still between 8.57 and 16.31 min for Anopheles vector, and resuming breeding for 24 h, the mortality was still 100%. ConclusionThere is strong deadly effect of Responsar to Anopheles sinensis and Anopheles anthropophagus, and the effect can keep more than 180 d.

Objective To study the relationship between P450 (CYP6 and CYP4) genes and deltamethrin resistance in Anopheles anthropophagus. Methods The real-time RT-PCR technique was used for semi-quantitative analysis of mRNA copy numbers of CYP6 and CYP4 gene from susceptible and deltamethrin-resistant strains. Results It is found that the mRNA copy numbers of CYP6 gene in deltamethrin-resistant strain were about 1. 39 times higher than those in susceptible strain, and the mRNA copy numbers of CYP4 gene in deltamethrin-resistant strain were about 3. 63 times higher than those in susceptible strain of Anopheles anthropophagus. Conclusion The higher expressions of CYP6 and CYP4 mRNA are observed in deltamethrin resistance strain and indicate that both CYP6 and CYP4 genes may be involved in the deltamethrin resistance in Anopheles anthropophagus.

Objective To study the effect of nursing intervention on preoperative anxiety of patients with gynecological carcinoma.Methods 60 patients with gynecological carcinoma in our hospital who were to receive operations were randomly distributed into the control group and the intervention group with 30 in each group.The control group was given routine nursing,while the intervention group received nursing intervention based upon routine nursing.Indexes such as preoperative anxiety,sleeping quality and satisfaction degree to nursing care were evaluated within them.Results Through targeted nursing intervention preoperative anxiety of patients in the intervention group alleviated,their confidence enhanced and satisfaction degree to nursing care improved compared to those of the control group.Conclusions Targeted nursing intervention can reduce preoperative anxiety of patients with gynecological carcinoma and increase satisfaction degree to nursing care.

Objective To obtain cDNA fragment of CYP6 gene from Anopheles anthropophagus. Methods The degenerate RT-PCR technique was used to amplify the CYP6 mRNA from susceptible and deltamethrin-resistant strains of An.anthropophagus. The specific cDNA fragments were sequenced and analyzed. Results The 250 bp specific cDNA fragments of CYP6 gene from both susceptible and resistant strains were cloned and sequenced by the Direct Cloning Method. Total of 10 sequences, including 2 from susceptible strain and 8 from resistant strain, were obtained. Compared with P450 sequence databases from GenBank, all of the 10 sequences obtained were new P450 sequence and were lodged in GenBank (Accession numbers: AY273927-AY273936). According to being checked up by Nomenclature Committee of Cytochrome P450, 7 of them were confirmed as the new CYP6 genes and the others were alleles and all of them belonged to the subfamilies CYP6Z, CYP6P, CYP6N and CYP6M in CYP6 family, respectively. Conclusion The cloned 10 sequences are gene fragments from members of CYP6 family.[

In 2006, there were 767 reported malaria cases in Jiangsu Province with an incidence of 1.07‰ and increased by 16.57% in comparison to the previous year. Positive rate of blood examination in local febrile patients was 0.08% (293/361 896) but 1.23% (251/204 40) in mobile population (P

Objective To evaluate the effect of automatic early warning information system on the response of malaria elimi-nation in Jiangsu Province through the operation of the national automatic early warning system of infectious diseases. Methods The malaria early warning information was collected from the automatic early warning information subsystem in the national infor-mation system for diseases control and prevention. Malaria early warning signals were analyzed from September 1 to December 31, 2012. The statistical analysis was conducted for the completion rates of case investigation within 3 days before and after the applica-tion of malaria early warning information system. Results Jiangsu Province received 85 mobile phone short messages(SMS)of malaria case from early warning system from September 1 to December 31,2012. After judgments,23 cases were deleted includ-ing 8 repeated cases and 15 cases that were excluded through the microscopy examination and epidemiological investigation by the confirmation of county CDC. From July to December in 2012,the monthly completion rates of case investigation within 3 days were 55.56%,78.57%,90.00%,100%,100%and 100%,respectively. The completion rates of case investigation within 3 days in Ju-ly,August,September and October were significantly different by χ2 test(χ2=10.66,P<0.05). The completion rates of foci in-vestigation and action within 7 days in Jiangsu Province were all 100%from July to December in 2012. Conclusions The comple-tion rates of case investigation within 3 days are associated with SMS from the early warning system. The malaria warning system from the national infectious diseases can effectively improve the response to malaria cases for primary CDC. It also plays an impor-tant role for the timely confirmation and diagnosis of malaria cases.

Objective To research the therapeutic effect of dihydroarteminsinin/piperaquine phosphate(duo-cotecxin)on rats with Pneumocystis pneumonia(PCP),and explain its potential mechanism.Methods The PCP rat model was established through the subcutaneous injection with cortisone acetate twice a week for 6 weeks.The model rats were orally treated with duo-cotecxin at a dose of 40 mg/kg once a day for 3 days,and with cotecxin at a dose of 60 mg/kg once a day for 6 days,respectively.The therapy control group was treated orally with SMZco(sulfamethoxazol 250 mg/kg +Trimethoprim 50 mg/kg),positive control group and normal group were established,respectively.The drug efficacy was based on rat survival rate,increase rate of body weight,lung weight/body weight ratio,mean numbers of the cysts per field in the lung print smear,and the changes of CD4+ T cells,CD8+ T cells,NO,IFN-? and TNF-? in the blood.Results The survival rates and the body weight of rats treated with duo-cotecxin and cotecxin were higher and heavier than those of the positive control group respectively,which approached to those of the group treated with SMZco.The lung weight/body weight ratio,and mean cyst number per field of lung print smear of the rats treated with duo-cotecxin and cotecxin were lower than those of the positive control group,respectively.NO and TNF-? in the blood of the rats treated with duo-cotecxin and cotecxin were lower than those of the positive control group,respectively(P

Objective To compare the susceptibility of Anopheles anthropophagus from Jiangsu, Guangdong and Liaoning provinces in China to Plasmodium vivax.Methods The blood samples of patients with P. vivax in endemic areas of China were collected to feed the mosquitoes of An. anthropophagus from different areas by using the artificial in vitro membrane feeding system in the lab, and then the mosquitoes were dissected during the 7-9th day and on the 14th day after the feeding and the oocysts and sporozoites in the stomach and salivy gland of mosquitoes were counted. Results The mosquitoes from Jiangsu, Guangdong and Liaoning were simultaneously fed with the blood of 35 cases of P. vivax. The oocyst positive rates of An. anthropophagus from Jiangsu, Guangdong and Liaoning during the 7-9th day after the feeding were 68.57%, 60.00% and 68.57%, as well as the sporozoite positive rates of them on the 14th day after the feeding were 22.86%, 14.29% and 22.86%, respectively. On the 7th day after the feeding, 228, 235, 228 mosquitoes of An. anthropophagus from Jiangsu, Guangdong and Liaoning were dissected, and the positive mosquito rates with oocyst infection were 28.07%, 25.11% and 26.75%, respectively. On the 14th day after the feeding, 150, 142, 135 mosquitoes of An. anthropophagus from the three areas were dissected, the positive rates with sporozoite infection were 10.67%, 8.45% and 11.85%, respectively. The num-bers of mosquitoes dissected with infective grade("+","++","+++","++++") of sporozoites of An. anthropophagus from Jiangsu, Guangdong and Liaoning were 4, 3, 2, 7; 2, 2, 3, 7 and 1, 6, 3, 8, respectively. Conclusions An. anthropophagus from Jiangsu, Guangdong and Liaoning is susceptible to the parasites of Plasmodium vivax and there is no significant difference among the susceptibilities of An. anthropophagus from the three areas to Plasmodium vivax.

Objective To establish a fast and sensitive method to detect Plasmodium sporozoites in mosquitoes. Methods Anopheles anthropophagus (An.a) artificially infected with Plasmodium vivax (P.v), Anopheles dirus (An.d) infected with Plasmodium falciparum (P.f) and both of P.v and P.f, field mosquitoes of Anopheles sinensis (An.s) captured in epidemic seasons were detected by nested-PCR. Results The results of 28 batches of An.a infected with P.v, 2 batches of An.d with P.f and 1 batch of An.d mixed with P.v and P.f by nested-PCR were accorded with the microscopical examination absolutely. Two positives of 589 An.s field mosquitoes were discovered with a positive rate of 0.34%. Conclusion The nested-PCR is fast and sensitive for detecting different species of Plasmodium in mosquitoes.

Objective To understand the malaria epidemic situation and characteristics in Jiangsu Province in 2012,so as to provide the evidence for formulating and adjusting effective malaria elimination strategies and measures. Methods The reported malaria cases from the Internet Reporting System and the epidemiological data of malaria in Jiangsu Province were collected and analyzed. Results A total of 198 malaria cases were reported in Jiangsu Province in 2012 with the incidence of 0.026/10 000, which decreased by 47.06%compared with that in 2011(374 cases). A total of 198 malaria cases were reported from 13 prefec-tures of Jiangsu and the cases were mainly distributed in Yangzhou(34 cases),Nantong(31 cases),Nanjing(22 cases),Tai-zhou(21 cases),Xuzhou(17 cases)and Huaian(17 cases),which accounted for 71.72%(142/198)among the total cases of the province. There were no local malaria cases reported from Jiangsu in 2012,and the imported malaria cases from other countries de-creased by 45.15%compared with that in 2011. Conclusions For the first time,there are no local malaria cases reported from Ji-angsu in 2012. However,the imported case distribution is further expanded and the infected plasmodium parasites are more di-verse. Imported malaria from other countries remains the key for malaria control in Jiangsu Province.