In 2006, there were 767 reported malaria cases in Jiangsu Province with an incidence of 1.07‰ and increased by 16.57% in comparison to the previous year. Positive rate of blood examination in local febrile patients was 0.08% (293/361 896) but 1.23% (251/204 40) in mobile population (P

ObjectiveTo observe the effectiveness of Responsar against Anopheles sinensis and Anopheles anthropophagus. MethodsThe cotton and nylon gauze absorbing Responsar solutions of 10,15 and 20 mg/m~2 (effective dosage) were used in the test. An. sinensis and An. anthropophagus raised in the lab were used as probational worms, having the knockdown power, lethiferous power and effective keeping observation. ResultsWith Responsar of 10,15 and 20 (mg/m~2) impregnating two different kinds of bednets, the KT_(50(s)) of An. sinensis and An. anthropophagus were between 3.98 and 7.30 min after touching the bednets. The knocked down mosquitoes were resumed breeding for 24 h, and the mortality was 100%. Touching nets 3 min, resuming breeding for 24 h, the mortality of mosquitoes was more than 90%. The nets hung for 180 d after impregnated with the insecticide and the knockdown power was still between 8.57 and 16.31 min for Anopheles vector, and resuming breeding for 24 h, the mortality was still 100%. ConclusionThere is strong deadly effect of Responsar to Anopheles sinensis and Anopheles anthropophagus, and the effect can keep more than 180 d.

Objective To study the relationship between P450 (CYP6 and CYP4) genes and deltamethrin resistance in Anopheles anthropophagus. Methods The real-time RT-PCR technique was used for semi-quantitative analysis of mRNA copy numbers of CYP6 and CYP4 gene from susceptible and deltamethrin-resistant strains. Results It is found that the mRNA copy numbers of CYP6 gene in deltamethrin-resistant strain were about 1. 39 times higher than those in susceptible strain, and the mRNA copy numbers of CYP4 gene in deltamethrin-resistant strain were about 3. 63 times higher than those in susceptible strain of Anopheles anthropophagus. Conclusion The higher expressions of CYP6 and CYP4 mRNA are observed in deltamethrin resistance strain and indicate that both CYP6 and CYP4 genes may be involved in the deltamethrin resistance in Anopheles anthropophagus.

Objective To study the effect of nursing intervention on preoperative anxiety of patients with gynecological carcinoma.Methods 60 patients with gynecological carcinoma in our hospital who were to receive operations were randomly distributed into the control group and the intervention group with 30 in each group.The control group was given routine nursing,while the intervention group received nursing intervention based upon routine nursing.Indexes such as preoperative anxiety,sleeping quality and satisfaction degree to nursing care were evaluated within them.Results Through targeted nursing intervention preoperative anxiety of patients in the intervention group alleviated,their confidence enhanced and satisfaction degree to nursing care improved compared to those of the control group.Conclusions Targeted nursing intervention can reduce preoperative anxiety of patients with gynecological carcinoma and increase satisfaction degree to nursing care.

Objective To obtain cDNA fragment of CYP6 gene from Anopheles anthropophagus. Methods The degenerate RT-PCR technique was used to amplify the CYP6 mRNA from susceptible and deltamethrin-resistant strains of An.anthropophagus. The specific cDNA fragments were sequenced and analyzed. Results The 250 bp specific cDNA fragments of CYP6 gene from both susceptible and resistant strains were cloned and sequenced by the Direct Cloning Method. Total of 10 sequences, including 2 from susceptible strain and 8 from resistant strain, were obtained. Compared with P450 sequence databases from GenBank, all of the 10 sequences obtained were new P450 sequence and were lodged in GenBank (Accession numbers: AY273927-AY273936). According to being checked up by Nomenclature Committee of Cytochrome P450, 7 of them were confirmed as the new CYP6 genes and the others were alleles and all of them belonged to the subfamilies CYP6Z, CYP6P, CYP6N and CYP6M in CYP6 family, respectively. Conclusion The cloned 10 sequences are gene fragments from members of CYP6 family.[

Objective To explore the effects of insulation measures on blood coagulation during laparotomy in the aged patients. Methods Sixty patients were randomly divided into the experimental group and the control group with 30 cases in each group from September 2007 to August 2008. The experimental group received a series of insulation measures based on general perioperative nursing care, which was given to the control group. Patients' temperature, platelet count (PLT), prothrombin time (PT), activated partial thromboplastin time (APTT), thrombin time (TV),fibrinogen (Fbg) were measured and these preand postoperative values together with blood loss during operation were compared between two groups. Results No significant change was seen in perioperative body temperature in the experimental group, the incidence rate of hypothermia was lower, the change in coagulation index was better than that of the control group. Conclusions These results suggested that mild hypothermia could influence aged patients' blood coagulation function and increase blood loss during laparotomy. Active insulation measures can maintain the stability of patients" temperature.

Objective To analyse the epidemic characteristics and control measures of malaria in Jiangsu Province during the past fifty years, evaluate the control effect and explore the current appropriate control strategy and measures. Methods The historical data about malaria cases and control measures from 1951 to 2008 in Jiangsu Province were collected, and the effects of malaria control in different stages were assessed. Results The integrated control measures with emphasis on the control of infectious source were adopted during the stage of two outbreaks of malaria. In the stage of basical elimination of malaria, the comprehensive measures with emphasis on the vector control in the areas of Anopheles anthropophagus were adopted, and the measures with emphasis on the surveillance of infectious source surveillance and early treatment of malaria in other areas were adopted. In the stage of consolidation, the measures including the surveillance of infectious source and management, surveillance of vector and epidemic situation, management of floating population, etc, were adopted. The malaria incidence dropped from 2 495/10 000 in 1960 to 0.09/10 000 in 2008, and was under 1/10 000 in all the counties of Jiangsu Province during the past 2 years, which demonstrated the various measures were feasible and effective in different stages of malaria. Conclusions Malaria has been controlled effectively in Jiangsu Province, however, the improvement of early diagnosis and norm treatment of malaria cases, the enhancement of health education and promotion, as well as the malaria surveillance and management among floating population, are still necessary for achieving the goal of malaria elimination.

Objective To observe the residual effect of Demand CS - impregnated bednets to Anopheles sinensis.Methods The numbers of adult mosquitoes which were knocked down at every minute after treatment were observed by using the enforced contact method recommended by WHO,and the mortality of mosquitoes tested after 24 h was calculated.Results The Kt_(50) of Demand CS - impregnated bednets with impregnations concentrations of 10,15,20 mg/m~2 to Anopheles sineasis were 21.40,16.20,15.70 min,respectively,and the 24 h mortality was 100%.The Kt_(50) of Responsar SC (12.5 % Beta - cyfluthrin) impregnated bednets with a impregnation concentration of 15 mg/m~2 to Anopheles sinensis was 16.06 min,and the 24 h mortality was 100%,too.Conclusions Both the Demand CS - impregnated bednets and the Responsar SC impregnated bednets can kill Anopheles sinensis.

Objective To analyze the genetic characteristics of ribosomal DNA ITS 2 region for differenciating anopheline mosquitoes within Hyrcanus group. Methods The ribosomal DNA ITS 2 region of both laboratory line and filed collected An. anthropophagus and An. sinensis as well as the field collected An. yatsushiroensis and An. lester i were amplified and sequenced. The sequencing data were then analyzed for the restriction mapping using Omega Sequencing analysis program. Results and Conclusion The length of the sequences of An. sinensis, An. anthropophagus, An. yatsushiroensis and An. lesteri are 472, 452, 456 and 456 bp respectively. The restriction mapping showed that there were different restriction digesting sites among the ribosomal DNA ITS2 region sequences from An. sinensis, An. anthropophagus, An. yatsushiroensis and An. lesteri . On the basis of the sequence differences among the anopheline species within Hyrcanus group, it is possible to develop new technique for genetic identification of anopheline mosquitoes.

Objective To understand the malaria epidemic situation and characteristics in Jiangsu Province in 2012,so as to provide the evidence for formulating and adjusting effective malaria elimination strategies and measures. Methods The reported malaria cases from the Internet Reporting System and the epidemiological data of malaria in Jiangsu Province were collected and analyzed. Results A total of 198 malaria cases were reported in Jiangsu Province in 2012 with the incidence of 0.026/10 000, which decreased by 47.06%compared with that in 2011(374 cases). A total of 198 malaria cases were reported from 13 prefec-tures of Jiangsu and the cases were mainly distributed in Yangzhou(34 cases),Nantong(31 cases),Nanjing(22 cases),Tai-zhou(21 cases),Xuzhou(17 cases)and Huaian(17 cases),which accounted for 71.72%(142/198)among the total cases of the province. There were no local malaria cases reported from Jiangsu in 2012,and the imported malaria cases from other countries de-creased by 45.15%compared with that in 2011. Conclusions For the first time,there are no local malaria cases reported from Ji-angsu in 2012. However,the imported case distribution is further expanded and the infected plasmodium parasites are more di-verse. Imported malaria from other countries remains the key for malaria control in Jiangsu Province.