1.The protective effects of extract of panax notoginseng saponins on retinal ganglion cells of the continuous high Intraocular Pressure in rats
Jianwen LI ; Jun LIU ; Zhitao ZENG ; Huayun HAN ; Hailong LI ; Wei LIU ; Xing ZHENG ; Hong LI
Chinese Journal of Primary Medicine and Pharmacy 2010;17(11):1441-1443,后插1
Objective To investigate protective effects and mechanisms of extract of PNS on retinal ganglion cells injury induced by continuous high intraocular pressure (IOP) in rats.Methods 80 healthy Sprague-Dawley ( SD) rats were randomly divided into four groups to establish rat model of high intraocular pressure with 4,8,12,16,20 weeks which there were cauterizing episcleral veins combined 5-Fu and only cauterizing episcleral veins.All the rats intraocular pressure was measured and recorded regularly.After 4,8,12,16,20 weeks,all rats were killed and the eyeballs were removed and to assay apoptosis of RGCs by TUNEL,to detect the activity of RGCs AgNOR staining and to discover the expression of caspase-9 by immunohistochemical detections.Results The IOP was almostly more than 36.55±0.27mmHg.The order of the number of TUNEL-positive cells in retinal ganglion cell layer,compared with the normal control group,there was a significant difference.Stained grains there was no significant difference between the combined treatment group and normal control group (P>0.05).The expression of caspase-9 protein in the saline group,treatment group and combined treatment group was obviously enhanced according to the normal control group.Conclusion The sustainable and stable rat model of high intraocular pressure could be established by cauterization of episcleral veins whih subconjunctival injection 5-Fu.PNS had significant protective effects in RCCs injury caused by the persistent high intraocular pressure.If controlling intraocular pressure with drugs which could lower the IOP,the protective effects of PNS on RGCs would be more prominent.PNS could inhibit the expression of Caspase-9 in the rat RGCs to protect RGCs.
2.Sample related factors affecting short-term culture of erythrocytic Plasmodium vivax in vitro
Feng LU ; Qi GAO ; Hui XIA ; Jun CAO ; Zhiyong TAO ; Guoding ZHU ; Huayun ZHOU ; Euntaek HAN ; Wencai JIANG ; Weiming WANG ; Yaping GU ; Julin LI
Chinese Journal of Schistosomiasis Control 2010;22(1):56-58,封3
Objective To explore the sample reIated factors affecting the short-term culture of erythrocytic Plasmodium dvax in vitro.Methods The vivax malaria blood samples were collected from the patients with malaria in endemic areas,and then incubated with McCoy's 5A medium in an incubator containing 5%CO_2 at 37℃.The factors affecting the short-term culture of Plasmodium vivax were analyzed.Results Plasmodium vivax could finish one asexual cycle in the selected medium.By analyzing the culture results of 74 samples.it was found that the factors affecting the short-term culture included long time delaying al room temperature(>4 h),single stage(only parasites in ring stage were found),patients taking antimalarials,antibiotics or sulfonamides.and low parasitemia.Conclusion The sample related factors are important to the short-term culture of erythrocytic Plasmodium vivax in vitro.
3.Prevalence of Drug Resistance-Associated Gene Mutations in Plasmodium vivax in Central China.
Feng LU ; Bo WANG ; Jun CAO ; Jetsumon SATTABONGKOT ; Huayun ZHOU ; Guoding ZHU ; Kwonkee KIM ; Qi GAO ; Eun Taek HAN
The Korean Journal of Parasitology 2012;50(4):379-384
Resistance of Plasmodium spp. to anti-malarial drugs is the primary obstacle in the fight against malaria, and molecular markers for the drug resistance have been applied as an adjunct in the surveillance of the resistance. In this study, we investigated the prevalence of mutations in pvmdr1, pvcrt-o, pvdhfr, and pvdhps genes in temperate-zone P. vivax parasites from central China. A total of 26 isolates were selected, including 8 which were previously shown to have a lower susceptibility to chloroquine in vitro. For pvmdr1, pvcrt-o, and pvdhps genes, no resistance-conferring mutations were discovered. However, a highly prevalent (69.2%), single-point mutation (S117N) was found in pvdhfr gene. In addition, tandem repeat polymorphisms existed in pvdhfr and pvdhps genes, which warranted further studies in relation to the parasite resistance to antifolate drugs. The study further suggests that P. vivax populations in central China may still be relatively susceptible to chloroquine and sulfadoxine-pyrimethamine.
Antimalarials/*pharmacology
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China
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Chloroquine/pharmacology
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DNA, Protozoan/chemistry/genetics
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Drug Resistance/*genetics
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Folic Acid Antagonists/pharmacology
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Genotype
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Humans
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Malaria, Vivax/epidemiology/*parasitology
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Plasmodium vivax/drug effects/*genetics/isolation & purification
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Point Mutation
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Polymorphism, Single Nucleotide/*genetics
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Prevalence
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Protozoan Proteins/genetics
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Sequence Analysis, DNA
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Tandem Repeat Sequences/*genetics
4.Retrospect and prospect of surgical treatment of pituitary adenoma in China from 1978-2019
Sen XIE ; Weiliang ZHAO ; Yipeng HAN ; Huayun WU ; Yahui YANG ; Guozhuan MIAO
Chinese Journal of Neuromedicine 2020;19(3):234-240
From 1978 to 2019, the cause of neurosurgery in China has developed rapidly, and the surgical treatment of pituitary adenoma has achieved good results.. This article focuses on the surgical treatment of pituitary adenomas from imaging techniques, surgical techniques and anesthesia techniques, and looks forward to the future.