The purpose of the present experiment was to observe the morphological characteristics and invasiveness of a transplantable cancer NO 27 (U 27) after serial passages about 20 years. The results were as follows: The morphological characteristics of this tumor strain was low-differentiated carcinoma which were demonstrated by light microscopy and transmission electron microscopy. In the cytoplasm the single irregular distribution of the tensile fibrils and some semi-desmosome between adjacent cells were discovered by transmission electron microscopy. Two types of cancer cells were observed on the surface of the cancer nest by scanning electron microcopy:Ⅰ), There were many microvilli on the surface of the first type cancer cells; 2). The surface of the cancer cells of the second type were more flat sometimes with single small blebs. Many cancer cells had been released from cancer cell clump after 3 days of intramuscular implantation. Later they actively invaded into the spaces between muscle fibers in the form of amebae. Some cancer cells stretched out their filopodia directly in contact with target cells. The degenerative and atrophic changes of muscle tissue appeared in the invasive area of cancer cells. Finally these changes were leading to the disappearance of the entire structure of the muscle with the replacement by cancer tissues.

In order to standardize the management of fixed assets in basic medical research, and to solve the problem ofone equipment with more than one code , we discussed the fixed assets coding of instrument and equipment in this paper.The existing equipment classification of the Institute of Basic Medical Sciences of the Chinese Academy of Medical Sciences was analyzed.Depending on application of the experiment and the principle of equipment, the novel fixed assets encoding dictionary of instrument and equipment is generated, which fits in the application and development of basic medical research.The managers may find the corresponding code quickly with the standardized equipment name.The new encoding dictionary may facilitate the classification of basic medical experimental instruments, prevent multiple coding situations of equipment and improve the management.

Surgical treatment for bone and soft tumors of pelvis and sacrum presents a big challenge, because of the complex anatomy of sacropelvic region, large tumor volume at presentation, rich blood supply to the tumor and visceral involvemen, et al. Therefore, surgical excision and reconstruction are technically difficult for sacropelvic tumors. Extensive intraoperative haemorrhage could be life-threatening, and this issue remains a major concern. How to effectively control bleeding during surgery is critical for successful operation and patient's favorable prognosis. Some previous attempts, such as interventional selective internal iliac artery embolization or manual ligation through an additional anterior approach, were tested to be ineffective. Inspired by the success of resuscitative endovascular balloon occlusion of the aorta (REBOA) which resemble an endovascular tourniquet for traumatic hemorrhagic shock, some researchers have applied this techinique to control surgical bleeding during pelvic or sacral tumor resection.The authors have performed REBOA for more than 1 500 sacropelvic tumr surgeries since 2003 in Peking University People's Hospital. The patient age, the diameter of femoral artery and aorta, atherosclerosis, as well as tumor location, volume and expansion and blood suppy, have to be thoroughly evaluated prior to REBOA administration. Admittedly, the application of REBOA do reduce intraoperative bleeding, shorten the operation duration, improve the safety of surgery, yet some complications were observed including local hematoma at the puncture site, acute arterial thrombosis, femoral artery pseudoaneurysm or occlusio, et al. The purpose of this study is to review the literature on REBOA administration in pelvic and sacral tumors excision, with the focus on its indications, performing procedure, the safety and efficacy, and complications. Moreover, in order to popularize the clinical application of aortic balloon occlusion in the future, we summarize our experience of abdominal aortic balloon occlusion over 10 years.