1.CONCENTRATION AND DISTRIBUTION OF SELENIUM IN 12 kinds of SEAWEEDS
Chinese Journal of Marine Drugs 1994;0(01):-
Concentration and distribution of selenium have been investigated in 12 kinds of seaweeds. It is found by determination that the selenium concentration in Phaeophyta is lower than in Chlorophyta and Rhodophyta. Selenium is predominately associated with amino acids and proteins.
2.A SURVEY OF THE METHODS OF EXTRACTION, ISOLATION AND DETERMINATION FOR GLYCOSAMINOGLYCANS
Chinese Journal of Marine Drugs 1994;0(01):-
The studies on glycosaminogiycans (GAG) from animals are carrying out extensively in the fields of biology.biochemistry and medicine. The methodology of GAG researches has been developed rapidly at present. The researches of GAG have just begun in China. Synthesizing a large number of references,this paper gives a brief introduction on the methods of extraction isolation and determination for GAG. And the latest methods and developing tendency are summarized as well.
3.DISTRIBUTION OF SELENIUM ASSOCIATED WITH BIOCHEMICAL FRACTIONS IN BLUE MUSSEL AND BAY SCALLOP
Chinese Journal of Marine Drugs 1994;0(01):-
Concentration,biochemical fractions of selenium have been investigated in the tissue organs of Blue mussel ( Mytilus edulis ) and Bay scallop ( Argopecten trradiaus ). It is found by determination that the selenium concentration in the mantle is higher than in other tissue organs of the Blue mussel and Bay scallop. Selenium is predominantely associated with amino acids and proteins. 64. 4 per cents to 70. 4 per cents of selenium was associated with insoluble proteins in the mantle of the Blue mussel and Bay scallop.
4.Effects of D-Polymannuronic sulfate on potential-dependent and/or receptor-operated calcium channel in rat aortic rings
Haibo ZHU ; Meiyu GENG ; Huashi GUAN
Chinese Journal of Marine Drugs 1994;0(03):-
The effects of D-polymannuronic sulfate (DPS) on the norepinephrine (NA) or potassium chloride (KCL)-induced contraction of rat aortic rings were studied in this paper. The results showed that DPS at the final concentration of Img ? L-1 inhibited the contraction of aortic rings e-voked by NA or KCL and shift doseresponse curves for NA or KCL to the right in a non-parallel fa-sion and depressed their maximal response, implicating that DPS afforded the noncompetitive antagonism on contraction of rat aortic rings induced by NA or KCL. This finding suggested that DPS exerted an inhibitory action of potential-dependent calcium channel and that of receptor-operated calcium channel in vascular smooth muscle.
5.A preliminary study on the antifatigue effect of sea cucumber extract
Yuqian HAN ; Xiao SUI ; Huashi GUAN ;
Chinese Journal of Marine Drugs 1994;0(02):-
Objective To study the antifatigue effect of sea cucumber exstract.Methods According to the experimental methods of animal antifatigue, three doses of sea cucumber extract were applied to mice and their antifatigue effects were compared statistically.Result 300 mg?kg -1 ?d -1 sea cucumber extract could significantly prolong pole climbing time in mice, increase the reserves of liver glycogen and the content of blood glucose, decrease the contents of blood urea nitrogen (BUN) and blood lactic acid after strenuous exercise. Conclusion The preliminary study showed that the sea cucumber exstract has some antifatigue effects.
6.A new sesquiterpene lactone from root of Baccaurea ramiflora
Jing XU ; Huashi GUAN ; Qiang LIN
Chinese Traditional and Herbal Drugs 1994;0(10):-
Objective To study the chemical constituents in the root of Baccaurea ramiflora.Methods Various chromatographic techniques were used to separate and purify the constituents.The structures of the compounds were elucidated by physicochemical properties and spectral analyses(UV,IR,ESI-MS,1H-NMR,13C-NMR,Dept90,Dept135,1H-1H COSY,1H-1H NOESY,HMQC,HMBC).Results A new compound was isolated and idertified.Conclusion This compound is a novel compound and named as epidihydrotutin.
7.IDENTIFICATION OF GLYCOSAMINOGLYCAN FROM THE BAY SCALLOP ARGOPECTEN IRRADIAUS BY AGAROSE GEL ELEC-TROPHORESIS
Changyun WANG ; Huashi GUAN ; Shuqing LIU
Chinese Journal of Marine Drugs 1994;0(02):-
Fraction F3-3-3 of glycosaminoglycan was isolated from the leftover bits of Bay scallop Argopecten irradiaus . Infrared spectra of F3-3-3 was similar to that of heparin. Agarose gel electrophoresis of this fraction was performed in the system of barbital buffer (0. 06 mol/L,pH 8. 6). It was found that F3-3-3 showed single band in the electrophoresis. Compared with the agarose gel electrophoresis of standard glycosaminoglycan, such as heparin, hyaluronic acid and chondroitin 6-sulfate, it indicated that the relative migration rate of the fraction was close to that of heparin.
8.IDENTIFICATION OF NEUTRAL MONOSACCHARIDES IN GLY-COSAMINOGLYCAN FROM THE BAY SCALLOP ARGOPECTEN IRRADIANS BY GAS CHROMATOGRAPHY
Changyun WANG ; Huashi GUAN ; Changhu XUE
Chinese Journal of Marine Drugs 1994;0(04):-
The glycosaminoglycan (GAG), isolated from Bay scallop Argopectenirradians, contains neutral monosaccharides besides hexosamines and hexosuronic acids. The monosaccharides obtained by alcoholysis with HCI-methylalcohol from the sample of GAG was trimethylsilanized with hexamethyldisilan and chlortrimethylsilan (HMDS ' TMCS = 2 : 1). And the trimethylsilyl derivatives of monosaccharides was determined by gas chro-matography. Compared the gas chromatography of the sample with that of standard monosaccharides, it was found that the GAG of the Bay scallop contains five neutral monosaccharides, viz glucose, galactose, xylose, fucose and rhamnose.
9.INHIBITORY EFFECTS OF D-POLYMANNURONIC SULFATE ON PROLIFERATION OF RAT VASCULAR SMOOTH MUSCLE CELLS AND ITS RELATED MECHANISMS OF ACTION IN VITRO
Haibo ZHU ; Meiyu GENG ; Huashi GUAN ; Juntian ZHANG
Acta Pharmaceutica Sinica 2001;36(1):19-24
AIM To investigate the inhibitory effects of D-polymannuronic sulfate (DPS) on the proliferation of rat vascular smooth muscle cells (VSMC) induced by basic fibroblast growth factor (bFGF) or interleukin-1 (IL-1) and its related mechanisms. METHODS Rat aortic smooth muscle cells pretreated with DPS in concentrations ranging from 0.001 μg*mL-1 up to 100 μg*mL-1 were incubated at 37℃ for 24 h, followed by addition of bFGF (50 ng*mL-1) or IL-1 (50 U*mL-1) for another 24 h. The effects of DPS on the proliferation of VSMC were evaluated by MTT assays. VSMC were pretreated with DPS in concentrations ranging from 0.001 μg*mL-1 up to 1 μg*mL-1, followed by addition of L-NAME (0.1 μg*mL-1) or bFGF (50 ng*mL-1) for 24 h. Supernatant nitric oxide (NO) was determined with NO assay kit, while supernatant angiotensin II (Ang II) and endothelin-1 (ET-1) were measured by radioimmunoassay. RESULTS DPS exerted antiproliferative effects at concentrations ranging from 0.01 μg*mL-1 to 10 μg*mL-1, and its maximal effect was observed at the concentration of 1 μg*mL-1. Also, the suppressing actions of DPS on the proliferation of VSMC were diminished by increasing the concentrations of bFGF or IL-1. Furthermore, DPS increased NO synthesis and decreased Ang II and ET-1 contents released from VSMC in a concentration-dependent manner. CONCLUSION DPS afforded the antiproliferative effects on bFGF- or IL-1-treated VSMC and its underlying mechanisms were associated with enhancement of NO synthesis and decrement of Ang II and ET-1 production/release in vitro.
10.PREPARATION OF BASIC ALUMINIUM 6-?-(CARBOXYME-THYL)CHITOSAN
Yingxia LI ; Shidong CHU ; Ni SONG ; Xiaoning ZHANG ; Huashi GUAN
Chinese Journal of Marine Drugs 2000;0(06):-
Basic aluminium 6 ? (carboxylmethyl) chitosan was prepared by reacting the solution of 6 ? (carb?xylmethyl) chitosan in water with basic aluminium chloride 〔Al(OH) 2Cl〕. The structure of product was confirmed by IR and atomic absoption spectroscopy.