AIM:To determine the effect of microRNA-363(miR-363) on HepG2 cells.METHODS:Bioin-formatic analysis was conducted to identify if the Mcl-1 was regulated by miR-363.The expression of Mcl-1 and miR-363 was detected by real-time PCR in normal liver cell line LO2 and hepatocellular carcinoma cell line HepG2, Huh7 and PLC. miR-363 was transfected into the HepG2 cells, and then the level of Mcl-1 was measured.The relative viability was evalua-ted by MTT assay after the HepG2 cells were transfected with miR-363, and the apoptosis was analyzed by flow cytometry with Annexin V/PI staining.RESULTS:Bioinformatic analysis identified that there was a putative target site in the Mcl-1 mRNA for miR-363.Transfection of miR-363 mimics suppressed Mcl-1 expression in the HepG2 cells.Transfection of miR-363 mimics inhibited the cell viability as well as inducing cell apoptosis in HepG2 cells.CONCLUSION: Over-ex-pression of miR-363 significantly inhibits the cell viability and induces apoptosis in HepG2 cells, and the mechanism may be related to the downregulation of Mcl-1 caused by miR-363 transfection.

The process of liver metastasis of colorectal cancer includes tumor cells invasion,survival in blood stream,extravasation to the liver and proliferation. It is involved in series of molecular markers,among which vascular endothelial growth factor and endothelial growth factor receptor are the major targets.

Objective To investigate the value of glycated hemoglobin(HbA1c)and urine trace albumin (u -ALB)content in early diagnosis of type 2 diabetic nephropathy.Methods 200 patients with type 2 diabetes were selected as diabetes group,and 30 cases of healthy people as control group.According to the content of HbA1c, the diabetes patients were re -divided into low group (HbA1c <7%),the median group(7.1%≤HbA1c≤10%) and high value group (HbA1c >10.1%).The levels of HbA1c and u -ALB were detected and the correlation between them was calculated.Results The values of HbA1c (8.85 ±1.22)% and u -ALB (88.3 ±12.4)mg/L in the diabetes group were significantly higher than those of the control group (t =10.88,54.25,all P <0.05);The level of HbA1c in the high,medium and low group[(11.02 ±1.37)%,(8.45 ±2.01)%,(6.88 ±1.23)%]were consistent with the levels of the respective levels of fasting glucose[(13.22 ±2.05)mmol/L,(9.25 ±1.28)mmol/L, (6.27 ±0.63)mmol/L].HbA1c and constituting the u -ALB levels were positively correlated in the high,medium and low group(r =0.452,0.512,0.452,all P <0.05).Conclusion The combined detection of HbA1c and u -ALB levels has important value for early diagnosis of type 2 diabetic nephropathy.

Objective To compare the clinical efficacy of minimally invasive removal of intracranial hematomas and medical con-servative treatment for hypertensive cerebral hemorrhage .Methods A total of 75 patients with hypertensive cerebral hemorrhage were collected and randomly divided into two groups according to the treatments :38 patients treated with conservative medicine and 37 patients treated with minimally invasive removal of intracranial hematomas based on standard drug therapy .Neurological impair-ment and recovery of self-care ability of patients in both groups before and after three weeks and six weeks of treatment were scored by National Institute of Health Stroke Scale (NIHSS) .Changes of diffusion tensor imaging before treatment and after two weeks of treatment were evaluated by fractional anisotropy value (FA) .Results There was no significant difference between neurological im-pairments of the patients in two groups before treatment .But the recovery degree of functional impairment and muscle nerve in min-imally traumatic treatment group was more significant than those in the conservative medical treatment group after treatments . There was significant difference between two groups (P< 0 .05) .Conclusion Minimally traumatic of hematomas is an effective method of treating hypertensive intracerebral hemorrhage .

Objective To investigate the expression of paired box 2 (Pax2) and E-cadherin in human renal cell carcinoma (RCC) and their correlation with clinicopathologic parameters.Methods The RCC tissue microarrays containing 85 renal cell carcinoma specimens and 35 normal kidney tissue specimens were used to detect Pax2 and E-cadherin expressions by immunohistochemistry.Results The positive expression rate of Pax2 was 77.6％ (66/85) in RCC specimens,which was significantly higher than that in 35 normal kidney tissue specimens (P ＜ 0.01).The positive expression rate of E-cadherin was 30.6％ (26/85) in RCC specimens,which was significantly lower than that in 35 normal kidney tissue specimens (P ＜ 0.01).The expression of Pax2 in RCC was significantly related to histological classification and pathological grade (P ＜ 0.05),while it was not related to the size of tumor and clinical stage (P ＞ 0.05).The expression of E-cadherin in RCC was significantly related to the size of tumor,histological classification,pathological grade and clinical stage (P ＜ 0.05).The positive expression rate of E-cadherin in survival group was significantly higher than that in death group (P ＜ 0.05).Conclusions The abnormal expressions of Pax2 and E-cadherin may play a crucial stage in development of human RCC.Detection of the expressions of Pax2 and E-cadherin can be used in the evaluation of malignant degree and prognosis of RCC.

Objective To explore the expression of glial cell line-derived neurotrophic factor (GDNF) in rats with spinal cord injury (SCI) after epidermal neural crest stem cells (EPI-NCSCs) transplantation.Method EPI-NCSCs were isolated from GFP transgenic rats for transplantation.The rat SCI model was made by NYU-II impactor (10 g 25 mm) at T10 level.Then 30 SD rats were randomly divided into blank injury group (group A),DMEM transplantation group (group B),and experimental group (group C).The EPI-NCSCs were transplanted into the injured region one week after SCI.In DMEM group,the DMEM/F12 was used to substitute for the EPI-NCSCs.No treatment was done in blank injury group.The locomotor function was appraised by BBB score every week after transplantation.At sixth week after transplantation,GDNF mRNA and protein expression was detected.Result The BBB score in experimental group was significantly higher than the other two groups from two weeks after transplantation (P＜0.05).The expression of GDNF mRNA and protein in experimental group was significantly higher than the other two groups (P＜0.05).There was no significant difference between blank injury group and DMEM transplantation group (P ＞ 0.05).Conclusion The expression of GDNF can be up-regulated by EPI-NCSCs transplantation,which may be one of the mechanisms for EPI-NCSCs repairing SCI.

Objective To apply Nutritional Risk Screening-2002(NRS-2002) to perform primary screening for nutritional risk in patients with esophageal cancer who undergo radiotherapy, and assess their nutritional status, and to investigate the value of NRS-2002 in such patients.Methods A total of 97 patients who were diagnosed with esophageal cancer and underwent radiotherapy in Zhejiang Cancer Hospital from January 2010 to April 2014 were analyzed retrospectively.The Kaplan-Meier method was applied to analyze the difference in survival, and the chi-square test and the Pearson correlation analysis were applied to analyze the correlation between NRS-2002 score and blood parameters.Results Of all patients, 26.8%had nutritional risk before radiotherapy, which gradually increased with the progress of radiotherapy.The 1-year overall survival rates of the patients with NRS-2002scores of ≤3 and ≥4 on admission were 91.1%and 61.9%, respectively (P=0.010).As for the patients with the highest NRS-2002 scores of ≤2 and ≥3 during treatment, the 1-year overall survival rates were 94.2% and 77.5%, respectively (P=0.012).As for the patients with the lowest NRS-2002 scores of ≤3 and ≥4 during treatment, the 1-year overall survival rates were 91.3% and 54.5%, respectively ( P=0.018).The NRS-2002 score was correlated with prealbumin on admission and at week 1 of radiotherapy (P=0.000 and 0.002), and the NRS-2002 score was correlated with albumin at week 3 of radiotherapy (P=0.036).The multivariate analysis showed that the TNM stage of esophageal cancer and the highest NRS-2002 score during treatment were the independent prognostic factors in esophageal cancer (P=0.001 and 0.005).Conclusions The patients with esophageal cancer undergoing radiotherapy have high nutritional risk, and NRS-2002 score is the independent prognostic factor in these patients and can be used as a tool for primary screening for nutritional risk.

Objective To observe the clinical efficacy of CYP2C19 genotype detection guided an-tiplatelet therapy in patients with ischemic stroke(IS)and its effect on adverse cardiovascular and ce-rebrovascular events(MACCE).Methods A total of 130 IS patients were selected in this study,and were divided into control group(n=65)and observation group(n=65)by random number table method.Patients in the control group were given aspirin and clopidogrel regimen,those in the observa-tion group were firstly tested for CYP2C19 genotypes,and were further divided into fast metabolism(CYP2C19*1/*1)group(n=30),medium metabolism(CYP2C19*1/*2,CYP2C19*1/*3)group(n=20)and slow metabolism(CYP2C19*2/*2,CYP2C19*3/*3,CYP2C19*2/*3)group(n=15)based on the test results.Patients in the fast metabolism group were treated with clopidogrel,those in the middle metabolism were still treated with clopidogrel,and those in the slow metabolism group were given aspirin treatment at the first day of treatment.All patients were followed up for 1 year.According to the occurrence of MACCE,the patients were divided into MACCE group(n=28)and non-MACCE group(n=102).Platelet aggregation was compared between the control group and the observation group.Modified RANKIN Scale(mRS)scores and the incidence of MACCE were compared between control group and observation group.Univariate analysis was used to explore difference of clinical data between MACCE group and non-MACCE group;independent risk factors for the prognosis of MACCE in patients with IS were analyzed by multiple Logistic regression analy-sis.Results After 1 month of treatment,the platelet aggregation rates in the control group and ob-servation group were lower than before treatment,the platelet aggregation rates in the fast metabolism group and middle metabolism group were lower than that in the control group,and the platelet inhi-bition rates in fast metabolism group and middle metabolism group were higher than that in control group(P＜0.05).There was no significant difference between the slow metabolism group and the control group in platelet aggregation rate and platelet inhibition rate(P＞0.05).After 1 month of treatment,the mRS scores of both groups were significantly decreased,and the observation group was lower than the control group(P＜0.05).The incidence of MACCE in the observation group was significantly lower than that in control group(P＜0.05).The incidence rates of MACCE in the fast metabolism group and middle metabolism group were lower than those in the control group,and the in-cidence of MACCE in the middle metabolism group was the lowest(P＜0.05).Logistic regression model showed that older age,hypertension,diabetes and left ventricular ejection fraction(LVEF)＜50％were independent risk factors for MACCE in IS patients(P＜0.05).Conclusion For patients with IS,CYP2C19 genotype detection and corresponding treatment for patients with different sub-types can effectively improve their platelet aggregation rate and inhibition rate,and reduce the oc-currence of MACEE,especially for patients with CYP2C19*1/*2 and CYP2C19*1/*3 types of medium metabolism who have the highest benefits.Independent risk factors for MACEE in patients with IS include older age,hypertension,diabetes and LVEF＜50％.Close clinical monitoring should be conducted to improve the prognosis of these patients.

Objective To observe the clinical efficacy of CYP2C19 genotype detection guided an-tiplatelet therapy in patients with ischemic stroke(IS)and its effect on adverse cardiovascular and ce-rebrovascular events(MACCE).Methods A total of 130 IS patients were selected in this study,and were divided into control group(n=65)and observation group(n=65)by random number table method.Patients in the control group were given aspirin and clopidogrel regimen,those in the observa-tion group were firstly tested for CYP2C19 genotypes,and were further divided into fast metabolism(CYP2C19*1/*1)group(n=30),medium metabolism(CYP2C19*1/*2,CYP2C19*1/*3)group(n=20)and slow metabolism(CYP2C19*2/*2,CYP2C19*3/*3,CYP2C19*2/*3)group(n=15)based on the test results.Patients in the fast metabolism group were treated with clopidogrel,those in the middle metabolism were still treated with clopidogrel,and those in the slow metabolism group were given aspirin treatment at the first day of treatment.All patients were followed up for 1 year.According to the occurrence of MACCE,the patients were divided into MACCE group(n=28)and non-MACCE group(n=102).Platelet aggregation was compared between the control group and the observation group.Modified RANKIN Scale(mRS)scores and the incidence of MACCE were compared between control group and observation group.Univariate analysis was used to explore difference of clinical data between MACCE group and non-MACCE group;independent risk factors for the prognosis of MACCE in patients with IS were analyzed by multiple Logistic regression analy-sis.Results After 1 month of treatment,the platelet aggregation rates in the control group and ob-servation group were lower than before treatment,the platelet aggregation rates in the fast metabolism group and middle metabolism group were lower than that in the control group,and the platelet inhi-bition rates in fast metabolism group and middle metabolism group were higher than that in control group(P＜0.05).There was no significant difference between the slow metabolism group and the control group in platelet aggregation rate and platelet inhibition rate(P＞0.05).After 1 month of treatment,the mRS scores of both groups were significantly decreased,and the observation group was lower than the control group(P＜0.05).The incidence of MACCE in the observation group was significantly lower than that in control group(P＜0.05).The incidence rates of MACCE in the fast metabolism group and middle metabolism group were lower than those in the control group,and the in-cidence of MACCE in the middle metabolism group was the lowest(P＜0.05).Logistic regression model showed that older age,hypertension,diabetes and left ventricular ejection fraction(LVEF)＜50％were independent risk factors for MACCE in IS patients(P＜0.05).Conclusion For patients with IS,CYP2C19 genotype detection and corresponding treatment for patients with different sub-types can effectively improve their platelet aggregation rate and inhibition rate,and reduce the oc-currence of MACEE,especially for patients with CYP2C19*1/*2 and CYP2C19*1/*3 types of medium metabolism who have the highest benefits.Independent risk factors for MACEE in patients with IS include older age,hypertension,diabetes and LVEF＜50％.Close clinical monitoring should be conducted to improve the prognosis of these patients.

Objective:To investigate the application value of establishing the differential diagnosis model of pulmonary tuberculosis using routine laboratory data.Methods:The retrospective study was conducted. The routine laboratory data of newly diagnosed patients with pulmonary tuberculosis and other pulmonary diseases in Beijng Jishuitan Hospital and Beijing Hepingli Hospital from May 2015 to November 2021were collected. According to the random numbers showed in the computer, all the 11516 patients were divided into training dataset and test dataset with a ratio of 9∶1. Four machine learning algorithms, Support Vector Machine, Random Forest, K-Nearest Neighbor and Logistic Regression, were used to build models and select features. The diagnostic accuracy of each model was verified by using the 10-fold cross-validation method and the performance of each model was evaluated by using the receptor operator of characteristic (ROC) curve.Results:Random Forest was selected as the optimal machine learning algorithm to build the best feature model in the study. According to importance scale of factors, the differential diagnosis model of pulmonary tuberculosis consisting of 37 non-specific test indexes. In the validation set and test set the accuracy and area under curve (AUC) of the models were 0.747 and 0.736, the sensitivity, specificity and accuracy were 68.03% and 68.75%, 70.91% and 67.90%, 70.30% and 68.12%, respectively.Conclusion:A key tool in the differential diagnosis model of pulmonary tuberculosis was established by routine laboratory data in combination with machine learning. The results of this study need to be further verified by more data from medical institutions.