Objective To investigate the effects of different hemostatic methods in laparoscopic ovarian cystectomy on ovary functions . Methods A total of 90 cases of laparoscopic ovarian cyst resection from June 2010 to June 2013 were selected .They were divided into bipolar electrocoagulation group , monopolar electrocoagulation group , and suture group according to the doctor ’ s favor.The FSH, LH, and E2 before and after surgery were compared among the three groups . Results All patients were followed up for more than 1 year.The rate of patients with menstrual disorder in monopolar electrocoagulation group [33.3%(10/30)] was significantly higher than that in bipolar coagulation group [10.0%(3/30),χ2 =4.812,P=0.028] and suture group [6.7%(2/30),χ2 =6.667,P=0.010].There was no significant difference between bipolar premature coagulation group and suture group (χ2 =0.000, P=1.000).There was no significant difference in FSH ,LH,and E2 among the three groups before and 1 month after surgery (P>0.05).There was no significant difference in FSH ,LH, E2 between bipolar premature coagulation group and suture group 6 months after surgery (P>0.05).In the monopolar electrocoagulation group , the FSH [(10.43 ±2.04) U/L] and LH [(18.58 ± 3.82) U/L] levels were significantly higher than those in the bipolar electrocoagulation group [(8.12 ±1.82) U/L and (13.31 ± 2.53) U/L, P<0.05], and in the suture group [(7.08 ±1.68) U/L and (12.61 ±2.68) U/L, P<0.05].The E2 level in the monopolar electrocoagulation group [(252.5 ±26.9) pmol/L] was significantly lower than that in the bipolar electrocoagulation group [(321.3 ±28.2) pmol/L, P<0.05] and in the suture group [(313.7 ±31.4) pmol/L, P<0.05]. Conclusion In laparoscopic ovarian cystectomy , bipolar coagulation hemostasis is safer than monopolar electrocoagulation hemostasis , being worthy of clinical application .

Objective Choroidal neovascularization(CNV) is the main cause of blindness in over 50-years-old population. To establish an available CNV animal model is helpful for us to understand the pathogenesis and management of CNV. Purpose of present study was to observe the role of coagulation with different power of diode laser in laser-induced choroidal neovascularization model of Brown Norway (BN) rats. Methods Coagulation of 810 nm diode laser(8 - 10 spots for each eye) was performed in 36 male BN rats with the spot diameter 75 μm, shutter time 0. 1 s, power 120 mW, 140 mW and 160 mW, respectively, while 6 normal BN rats were used as contrast. CNV was evaluated by fundus examination, fundus fluorescein angiography (FFA), indocyanine green angiography(ICGA) and light microscope on day 1, 7, 14, 21, 28 and 56 after photocoagulation. Results CNV formed on the 7th day after photocoagulation in 120 mW, 140 mW and 160 mW groups and reached the peak on the 21st day according to FFA and ICGA manifestation. Incidence of CNV in 120 mW, 140 mW and 160 mW group was 51. 3%, 91. 8%, 88. 3% on FFA findings and 51.3%, 92.7%, 93.7% on ICGA findings, respectively. In 7 days after photocoagulation, inflammatory cells increased and CNV formed at the lesion. Photocoagulation plaque became thicker with pigment cells proliferating and migrating on 14 days. After that time, inflammatory cells decreased and more collagen fibers emerged. The CNV reminded till the 56th day after photocoagulation. Conclusion CNV model of BN rats can be successfully created using the different power of diode laser (from 120 through 160 mW). CNV rate under the laser coagulation with 140 mW is higher, indicating that the power of 140 mW may be a suitable parameter for diode laser-induced choroidal neovascularization model of BN rats.

BACKGROUND: The presence of fibrosis is a criterion for subtype classification in the newly updated hypersensitivity pneumonitis (HP) guidelines. The present study aimed to summarize differences in clinical characteristics and prognosis of non-fibrotic hypersensitivity pneumonitis (NFHP) and fibrotic hypersensitivity pneumonitis (FHP) and explore factors associated with the presence of fibrosis. METHODS: In this prospective cohort study, patients diagnosed with HP through a multidisciplinary discussion were enrolled. Collected data included demographic and clinical characteristics, laboratory findings, and radiologic and histopathological features. Logistic regression analyses were performed to explore factors related to the presence of fibrosis. RESULTS: A total of 202 patients with HP were enrolled, including 87 (43.1%) NFHP patients and 115 (56.9%) FHP patients. Patients with FHP were older and more frequently presented with dyspnea, crackles, and digital clubbing than patients with NFHP. Serum levels of carcinoembryonic antigen, carbohydrate antigen 125, carbohydrate antigen 153, gastrin-releasing peptide precursor, squamous cell carcinoma antigen, and antigen cytokeratin 21-1, and count of bronchoalveolar lavage (BAL) eosinophils were higher in the FHP group than in the NFHP group. BAL lymphocytosis was present in both groups, but less pronounced in the FHP group. Multivariable regression analyses revealed that older age, <20% of lymphocyte in BAL, and ≥1.75% of eosinophil in BAL were risk factors for the development of FHP. Twelve patients developed adverse outcomes, with a median survival time of 12.5 months, all of whom had FHP. CONCLUSIONS Older age, <20% of lymphocyte in BAL, and ≥1.75% of eosinophil in BAL were risk factors associated with the development of FHP. Prognosis of patients with NFHP was better than that of patients with FHP. These results may provide insights into the mechanisms of fibrosis in HP.
Humans ; Bronchoalveolar Lavage Fluid ; Prospective Studies ; Alveolitis, Extrinsic Allergic/diagnosis* ; Fibrosis ; Carbohydrates