ObjectiveTo investigate the expression of adhesion molecules cyclooxygenase-2 (COX-2)and survivin protein in elderly patients with gastric cancer and their relationship with the pathological behavior and prognosis,MethodsImmunohistochemical staining was used to detect the expression of COX-2 and survivin protein in 60 cases of elderly patients with gastric carcinoma,30 cases of gastric epithelial dysplasia and 20 cases of normal gastric mucosa.The study was also combinedwith analysis of the pathological behavior and clinical follow-up survey of elderly patients with gastric carcinoma.ResultsThe positive expression rates of COX2 and survivin protein in elderly patients with gastric carcinoma were 63.3％(38/60) and 70.0％(42/60),respectively.The positive rates of COX-2 and survivin in elderly patients with gastric carcinoma were higher than that in epithelial dysplasia and normal gastric mucosa( P ＜0.05).The expression of COX-2 and survivin were closely related to the infiltration of serosa,metastasis of lymph node and prognosis of patients ( P ＜ 0.05 ).ConclusionThe expression of COX-2 and survivin are closely related to carcinogenesis,metastasis and survival time in elderly patients with gastric carcinoma.Detecting the expression of COX-2 and survivin may be prognostic indicators of elderly patients with gastric carcinoma.

ObjectiveTo explore the correlation of expression of galetin-3 (Gal-3) and epidermal growth factor receptor (EGFR) with the biological behaviors in esophageal squamous carcinoma(ESC). MethodsImmunohistochemical method was used to examine the expression of Gal-3 and EGFR in 80 specimens of ESC. ResultsThe positive rates of Gal-3 and EGFR was 73.8％ (59/80)and 56. 3％ (45/80)respectively. The positive rates of Gal-3 and EGFR expression in the patients with poor differentiation, deep infiltration and lymph node metastasis was significantly higher than those with well differentiation, shallow infiltration and without lymph node metastasis ( P ＜ 0. 05 ). The high expression of Gal-3 and EGFR was correlated with higher percentage of death and lower survival rate ( P ＜ 0.05 ). ConclusionThe abnormal expression of Gal-3 and EGFR is closely related with the pathological biology behaviors of ESC. Gal-3 and EGFR can be regarded as objective indicators for forecasting metastasic potential of ESC and estimating prognosis of patients with ESC.

Objective To investigate the expression of CA242 and CD_(44v6) protein in gastric cancer (GC) and its clinical significance. Methods Immunohistochemical staining was used to detect the expression of CA242 and CD_(44v6) protein in 120 cases of GC, 30 cases of gastric epithelial dysplasia and 20 cases of normal gastric mucosa. The pathological feature of GC and clinical follow-up data was also analysed. Results The positive rate of CA242 and CD_(44v6) was 79.2 % and 81.7 %, respectively in primary GC. The positive rate of CA242 and CD_(44v6) in GC was higher than that in epithelial dysplasia and normal gastric mucosa (P <0.05). The expression of CA242 and CD_(44v6) was closely related to infiltration of serosa, metastasis of lymph node and prognosis of patients (P <0.05). Conclusion These data suggest that the expression of CA242 and CD_(44v6)is closely related to carcinogenesis, metastasis and survival rate in GC. CA242 and CD_(44v6) may be prognostic indicators of GC.

Objective To investigate the correlation between the expression of cyclooxygenase-2 (COX-2),vascular endothelial growth factor(VEGF) protein,and metastasis in human papillary thyroid carcinoma (PTC).Methods lmmunohistochemical method was used to detect the expression of COX-2 and VEGF protein in 66 cases of PTC,20 cases of thyroid adenoma (TA),20 cases of nodular goiter (NG) and 15 cases of adjacent noncancerous (AN) tissues.Results The positive expression rate of COX-2 (72.7％,48/66)and VEGF (59.1％,39/66) was significantly higher in PTC than in TA( 10.0％,15.0％ ),NG( 15.0％,20.0％ )and AN (0.0％,13.3％ ) (P ＜0.05).The expression of COX-2 and VEGF was closely related to invasion,metastasis,and survival duration of PTC patients (P ＜0.05).Conclusion The expression of COX-2 and VEGF is closely related to biological behaviors of PTC patients and can be taken as prognostic indicators for PTC patients.

Objective To investigate the expression of survivin and CD44v6 protein in gastric cancer(GC) and its clinical significance.Methods Immunohistochemical staining was used to detect the expression of survivin and CD44v6 protein in 120 cases of GC,30 cases of gastric epithelial dysplasia(GED) and 20 cases of normal gastric mucosa(NGM).The pathological features of GC and clinical follow-up data were also analyzed.Results The positive rate of survivin and CD44v6 was 75.8% and 81.7%,respectively,in primary GC.The positive rate of survivin and CD44v6 in GC was higher than that in GED and NGM(P

Objective　To investigate the level of cell adhesion molecule sialyl-LeX expression in colorectal carcinoma and its relation with carcinogenesis, differentiation, metastasis and prognosis. Methods　Sialyl-LeX expression and its optical density in colorectal carcinoma (n=90) and remote normal mucosa (n=30) were quantitatively studied with microwave-LSAB immunohistochemical method combined with image analysis technique. Fifty-three patients were followed up. Results　 The weaker staining in remote normal colorectal mucosa was observed in very limited parts of some deep crypts. Positive rate of sialyl-LeX expression was only 16.7%(5/30). The positive expression of sialyl-LeX was observed in 83 of 90 patients with colorectal carcinoma(92.2%). The apical cytoplasma of cancer tubules, the luminal contents, and the cytoplasma of the cancer cells were strongly stained. The mean integral optical density of sialyl-LeX positive cell in poorly differentiated adenocarcinoma was significantly higher than that in highly differentiated and mucinous ones (P<0.01). It was markedly higher in patients with positive lymphatic nodes than that in negative ones (P<0.01). With followed-up for longer than 5 years, it was much lower in the alive cases than that in the dead (P<0.01). Conclusion　These findings indicate that changes of sialyl-LeX expression and its optical density is related to carcinogenesis, differentiation, invasion and metastasis of colorectal carcinoma. It may be a good predicter for the prognosis of patients with colorectal carcinoma.

Objective:In order to study the correlation between The expression of CD44v6 protein and Bcl-2 protein in thyroid cancer were examined.Methods:The expression of CD44v6 and Bcl-2 in 50 thyroid cancers,20 adjacent noncancerous portion,45 adenoma and 10 normal thyroid tissue were respecitively investigated by catalyzed signal amplification immunohistochemical technique. Results:The positive rate of CD44v6 and Bcl-2 in thyroid cancer was 64.0% and 46.0%, which was significantly higher than that in adenoma or adjacent noncancerous ( P

Purpose:To investigate the relationship of expression of CD44v6, E cadherin (E Cad) with clinic biologicl behavior in breast cancer and its correlation. Methods:Catalyzed signal amplification immunohistochemical method was used to detect the expression of CD44v6 and E Cad in 94 cases of breast cancer.Results:The positive expression of CD44v6 and the negative expression of E Cad were positively correlated with the grading, clinical stage, lymph node metastasis recurrence and prognosis of breast cancer. In breast cancer, the expression of CD44v6 had negative correlation to that of E Cad.Conclusions:Several genes were involved in the occurrence and development of breast cancer in which the expression of CD44v6 had negative regulative synergistic action with that of E Cad. CD44v6 and E Cad expression, as a clinical valuable marker, has a great significance in assessing node metastasis and survival.

Objective To investigate the expression of S100A4 protein in benign and malignant thyroid tissues and its clinical significance. Methods Expression of S100A4 protein in thyroid cancers (50 cases), adenoma (45 cases) and adjacent noncancerous tissue (20 cases) was respectively studied by microwave-LSAB immunohistochemical staining. Results The positive rate of S100A4 protein in thyroid cancer and adenoma was 78.0% and 15.6% respectively. No S100A4 protein expression was found in any adjacent noncancerous tissue (P ＜0. 01). No correlation was observed between expression of S100A4 protein and histological grading of thyroid cancer. Expression of S100A4 protein in lymph node metastasis and staging Ⅲ-Ⅳ was both higher than that in negative cases and staging Ⅰ -Ⅱ (P ＜0. 05). The rate of recurrence and death in S100A4 protein positive cases were notably higher than that in negative cases (P ＜ 0. 05). Conclusions S100A4 protein expression can help to distinguish benign and malignant thyroid tissues. It can be used as a molecular marker to predict thyroid metastasis.

This study was conducted to study the expressions of sialyl Lewis X and P16 protein in colorectal carcinoma and their relationship with differentiation, metastasis and prognosis of colo rectal carcinoma. Sialyl Lewis X and P16 expressions were determined with microwave-LSAB immunohistochemical technique. The positive rates of sialyl Lewis X and P16 were 92.2% and 42.2% respectively. The increased expression of sialyl Lewis X and the weakened expression of P16 were significantly correlated with metastatic proclivity and poor prognosis of the malignancy. These findings indicated that combined examination of the expression of sialyl Lewis X and P16 might be useful in estimating the differentiation, infitrtion, metastasis of colo-rectal carcinoma and prognosis of the patients.\;