1.Application situation of degradable coronary stents
Lufeng LI ; Huanyun LIU ; Xiaohui ZHAO
Chinese Journal of Tissue Engineering Research 2014;(8):1270-1276
BACKGROUND:The appearance of biodigradable stents brings a new dawn for the fourth coronary intervention revolution. They not only can solve the problem of postoperative acute occlusion of blood vessels, but also can be completely absorbed after a certain time.
OBJECTIVE: To summarize the application status of biodegradable coronary stents.
METHODS:PubMed, CBM and embase were searched for articles related to biodegradable intravascular stents.
RESULTS AND CONCLUSION:Biodegradable polymer stents, biodegradable magnesium stents and biodegradable iron stents are currently the three major research biodegradable stent systems. Numerous clinical trials have demonstrated the long-term safety and reliability of the biological degradation stents, and in the near future they wil replace the existing drug-eluting stents as the primary means of percutaneous coronary intervention. Biodegradable stents currently stil have their limitations, which are reflected in the relationship between mechanical properties and degradation rate and cannot be applied to complicated coronary patients temporarily. It takes 6-12 months to restore normal physiological function of blood vessels, and it can be considered reasonable that degradation of stents is completed in 12-24 months. Poly(lactic-co-glycolic) produced by polylactic acid and polyglycolic acid is currently widely recognized as the stent’s framework. We can get a more appropriate balance between the mechanical properties of the stent and the degradation rate by adjusting the ratio of polylactic acid and polyglycolic acid. This stent not only has good mechanical properties, but also can be completely biodegradable after the restoration of normal function of blood vessels, which has a broad research space.
2.Researching situation on salpingemphraxis infertility treated with traditional Chinese medicine
Baozhen PANG ; Qingyang PANG ; Huiqing PANG ; Huanyun ZHAO
International Journal of Traditional Chinese Medicine 2010;32(5):457-459
Objective To explore the rule for treating salpingemphraxis infertility with TCM . Methods A comprehensive analysis was made based on relative literatures and self clinical experience. Results TCM had experienced a relatively big progress in treating salpingemphraxis infertility from etiology, treatment based on syndrome differentiation,combined therapy of syndrome differentiation and disease differentiation, specific formula for specific disease, acupuncture,massage, paste of Chinese medicine, and lab researches. Conclusion TCM had sound clinical effects in treating salpingemphraxis infertility.
3.Preparation of pitavastatin-loaded poly lactic-co-glycolic acid nanoparticles and their effects on proliferation of endothelial progenitor cells
Huanyun LIU ; Lufeng LI ; Chunxin XU ; Mengyang DENG ; Xiaohui ZHAO
Journal of China Pharmaceutical University 2016;47(2):166-170
The objectives of this study were to prepare pitavastatin-loaded poly lactic-co-glycolic acid nanoparticles(PLGA), to characterize their pharmaceutical properties, to conduct in vitro drug-release from the nanoparticles, and to observe the effects on the proliferation of endothelial progenitor cells. Both pitavastatin-loaded PLGA and blank PLGA nanoparticles were prepared using emulsion-solvent diffusion method with PLGA being carrier materials. Morphology of the nanoparticles was observed by scanning electron microscopy(SEM), and particle size was analyzed by laser nanometer particle size analyzer. The drug loading and encapsulation efficiency were assayed using high-performance liquid phase. Impact of blank and pitavastatin-loaded nanoparticles on the viability of endothelial progenitor cells was investigated by CCK8 method. Pitavastatin-loaded PLGA nanoparticles exhibited the structure with spherical shape, smooth surface and average diameter of(230. 1±45)nm. The drug loading capacity and encapsulation efficiency were(10. 00±1. 83)% and(35. 54±5. 40)%, respectively. In vitro sustained-release of pitavastatin from the nanoparticles was found. The blank PLGA nanoparticles had no effect on the viability of the endothelial progenitor cells in different concentrations. Compared with pitavastatin group, pitavastatin-loaded nanoparticles(0. 01 μmol/L, 0. 1 μmol/L)had more effects on the proliferation of endothelial progenitor cells. In conclusion, emulsion-solvent diffusion method is applicable in preparation of pitavastatin-loaded PLGA nanoparticles with good shape and sustained-release of interest. Pitavastatin-loaded nanoparticles could significantly improve proliferation of the endothelial progenitor cells.
4. Effects of zinc ions on biological functions of human umbilical vein endothelial cells
Pang BAO ; Huanyun LIU ; Yuqing WANG ; Yajun TAN ; Lufeng LI ; Chunxin XU ; Lan HUANG ; Xiaohui ZHAO
Chinese Journal of Cardiology 2018;46(5):390-395
Objective:
To evaluate the effect of zinc ions on human umbilical vein endothelial cells biological functions.
Methods:
The primary human umbilical vein endothelial cells were cultured with the ECM medium, and cells were divided into 8 groups: the control group(routine culture,
5.Establishment of UPLC fingerprint and study on anti-respiratory syncytial virus spectrum-effective relationship of ethanol extract from Callicarpa nudiflora
Bingmei SHAO ; Xiaofeng LI ; Beibei YAN ; Qifeng LIU ; Huanyun QIN ; Xiangjun ZHAO ; Lin HOU
China Pharmacy 2023;34(20):2465-2470
OBJECTIVE To establish the fingerprint of the ethanol extract from Callicarpa nudiflora, analyze its anti- respiratory syncytial virus (RSV) activity in vitro, and study the relationship between spectrum and effect. METHODS Using 10%, 30%, 50%, 70% and 90% ethanol as solvent, 20 batches of ethanol extracts from 4 batches of C. nudiflora were prepared. The fingerprints for 20 batches of ethanol extracts from C. nudiflora were mapped by ultra-high-performance liquid chromatography (UPLC), and the similarity evaluation was conducted by using the Similarity Evaluation System for Traditional Chinese Medicine Chromatographic Fingerprints (2012 edition). The cytopathic effect method and MTT method were used to investigate the in vitro inhibitory activity of the ethanol extracts from C. nudiflora on RSV. Pearson correlation analysis, grey correlation degree and orthogonal partial least squares (OPLS) analysis were used to study the spectrum-effect relationship. RESULTS There were 25 common peaks in 20 batches of ethanol extracts from C. nudiflora, and the similarities ranged from 0.912 to 0.998, and the RSDs of common peak areas were 33.54%-162.28%. The average values of IC50 for RSV of 20 batches of ethanol extracts from C. nudiflora were 9.55-272.23 μg/mL. The results of Pearson correlation analysis, grey correlation analysis and OPLS analysis showed that the Pearson correlation coefficients (P<0.05) of the common peaks 8, 10, 12, 16, 18-19, 22-24 with pharmacodynamic indicators and regression coefficients were all negative, the correlation coefficients were all greater than 0.6, and the values of variable importance in projection were all greater than 1. CONCLUSIONS Twenty batches of ethanol extracts from C. nudiflora have similar components but significant differences in content, and exhibit different degrees of anti-RSV activity in vitro. The corresponding components of common peaks 8, 10, 12, 16, 18-19, 22-24 may be the characteristic components of anti-RSV of C. nudiflora.
6.Study on the differential markers of Morus alba leaves before and after baked with honey based on fingerprint and chemometrics
Beibei YAN ; Xiaoping ZHANG ; Huanyun QIN ; Xiangjun ZHAO ; Bingmei SHAO ; Jingzhen TIAN ; Dalong MA
China Pharmacy 2024;35(10):1198-1202
OBJECTIVE To compare the changes of chemical components of Morus alba leaves, screen differential markers, and determine their contents, so as to provide reference for quality control of M. alba leaves before and after baked with honey. METHODS The fingerprints of M. alba leaves before and after baked with honey were established by high-performance liquid chromatography (HPLC). The common peaks of the fingerprints were identified and the similarity was evaluated. The differential markers of M. alba leaves before and after baked with honey were screened by principal component analysis (PCA) and orthogonal partial least squares-discriminant analysis (OPLS-DA) using common peak are of raw material and product baked with honey of M. alba leaves as index. The quantitative analysis was carried out. RESULTS Twenty-three and twenty-four common peaks were identified from the HPLC fingerprint spectra of ten batches of raw material and ten batches of product baked with honey of M. alba leaves, respectively. The similarities of HPLC fingerprints for raw material and product baked with honey of M. alba leaves were all greater than 0.97. The results of PCA showed that raw material and product baked with honey of M. alba leaves could be divided into two categories. The results of OPLS-DA showed that the variable importance in projection of peak 2, peak H (5- hydroxymethylfurfural), peak 1, peak 17 (isochlorogenic acid C) and peak 16 were all greater than 1. The average contents of differential marker of isochlorogenic acid C in raw material and product baked with honey of M. alba leaves were 0.093 6 and 0.127 8 mg/g, respectively; there was statistical significance (P<0.05). CONCLUSIONS Five differential markers such as isochlorogenic acid C are obtained. The content of isochlorogenic acid C in M. alba leaves is significantly increased after baked with honey.