Objective To understand the status of basic compensation for nurses′night-shift and nursing age allowance, and provide reference basis for government to make policy. Methods A total of 956 nurses of 22 hospitals from 18 cities in Henan were investigated and analyzed by the Nurse Night-shift Compensation Status Questionnaire. Results The human resources allocation of 71.9% (687/956) respondents was not up to standard. The nurses were under labor intensity. 60.06%(574/956) nurses needed to go to the night-shift for 2-3 times every week. 86.21%(824/956) nurses thought the intensity of labor was mild light or relatively high. The swing night shift compensation of 16.1% (153/956) nurses was none. The graveyard shift compensation of 52.1%(498/956) nurses was 1 to 10 yuan. 90.49%(865/956) nurses thought inked the compensation for night shift was unreasonable. 26.31% (252/956) nurses didn′t receive any payment for the night shift. Conclusions The managers in hospital should attach importance to the legitimate rights and interests of the nurses, optimize the management of human resources and improve the status of nurse shift rotation. They also need to perfect the management of nursing project cost to reflect the nurse work value.

0bjective To determine the immunogenicities of DNA vaccines expressing tat-rev-integrase(c-half)-vif-neffusion genes(TRIVN) derived from prevalent B', B'/C and AE recombinant subtypes of HIV-1 in China. Methods Two DNA vaccines were constructed by inserting the codon optimized tat-revintegrase(c-half)-vif-nef fusion genes derived from B' and B'/C subtype of HIV-1 into mammalian expression vector pSVI. 0. DNA vaccine containing tat-rev-integrase (c-half)-vif-nef fusion gene derived from HIV-1AE2f has been constructed previously. In vitro expression efficiencies of three DNA vaccines were determined by Western blot and their immunogenicities were compared by immunizing female BALB/c mice. IFN-γ ELISPOT assay was used to read out the specific T cell immunity. Results The constructed DNA vaccines were validated by restriction enzyme digestion and DNA sequencing. Western blot assay showed three constructed DNA vaccines could be expressed at a comparable level in vitro. After vaccination, AE-TRIVN mounted T cell immune responses at (948.0 ± 330.0) SFCs/106 splenocytes, followed by the mixed DNA vaccine[ (500.0 ± 155.0) SFCs/106 splenocytes ], RL-TRIVN r[ ( 195. 1 ± 44.0) SFCs/106 splenocytes ]and CN-TRIVN [ (89.5 ± 17.0) SFCs/106 splenocytes]. Interestingly, we observed that single DNA vaccination induced specific T cell responses predominantly targeting Integrase (C-half) and Vif, whereas the mixed DNA could significantly improve T cell responses against Nef. Conclusion AE-TRIVN was the most immunogenic among the three DNA vaccines and the mixed DNA vaccination could change the immunogenic hierarchy of T cell epitopes across the fusion genes vaccine.

Objective To study the effects of missense mutation of H4 Lysine 20,expore the molecular mechanisms,and find some specific interaction proteins,such as H4K20me3 demethyhransferase.Methods Stable HeLaS3 cell line expressed H4 or H4 mutations that fuses a Flag-HA tag to carboxyl(C)-terminal was established by virus infection.Mutations include lysine mutates to methionine and alanine.Harveste cells expressed purpose protein.Staining with propidium iodide (PI) and analyze cell cycle analysis by fluorescence activating cell sorter (FACS).Prepare mono nuclesome (mono-N) of three cells samples and purify associated protein complex by immunoprecipitation assay.Distingwish protein differences by Siliver staining and cut different protein bands for mass spectrometry analysis.Results HeLa S3 overexpressed H4K20M exhibit abnormal phenotype when transfected virus 72h,mainly show abnormal cell and nuclei volume increases,stop growing and no longer split.FACS shows cells overexpressed H4K20M were blocked in G2/M period.Mass spectrometry data shows the expression of PRBP4/7 increases in cells overexpressed H4K20M.Conclusion Histone H4 lysine 20 mutation to methionine block cell cycle,this block is likely to be caused by protein PRBP4/7.

Objective:To investigate the advantages and disadvantages of percutaneous compression plate (PCCP) for femoral neck fractures in the elderly patients.Methods:A retrospective study was conducted of the 31 elderly patients with femoral neck fracture who had been treated with PCCP from January 2012 to December 2018 at Orthopaedic Department, The First People's Hospital of Yancheng, Orthopaedic Department, The Ninth People's Hospital of Wuxi and Department of Orthopaedics, The People's Hospital of Wuxi. They were 16 men and 15 women, aged from 65 to 80 years (average, 70.5 years). By the Garden classification, 7 cases were type Ⅱ, 15 cases type Ⅲ and 9 cases type Ⅳ; by the Singh index, 4 cases were level Ⅲ, 11 cases level Ⅳ, 10 cases level Ⅴ and 6 cases level Ⅵ. The time from injury to operation ranged from 3 to 14 days (average, 5.8 days). The operation time, intraoperative blood loss, fracture reduction, fracture union time, complications and functional recovery of the hip were observed.Results:The operation time averaged 73.4 min and intraoperative blood loss 116.4 mL. At one week after operation, the Garden alignment index was level Ⅰ in 25 cases and level Ⅱ in 6. Superficial incision infection was noted in one case intraoperatively. Follow-ups for the 31 patients ranged from 12 to 47 months (mean, 18.9 months). All the fractures united after 4.9 months on average (from 4 to 8 months). Delayed union occurred in 2 cases, neck shortening in 12 cases, and avascular necrosis of femoral head in 3 displaced subcranial fractures 2 of which were high shear ones. The necrosis of femoral head was treated by arthroplasty in 2 and by conservative treatment in one. The Harris hip scores at the last follow-up for the 31 patients averaged 90.9 (from 75 to 100), giving 15 excellent, 12 good and 4 fair cases and an excellent to good rate of 87.1% (27/31).Conclusions:In treatment of femoral neck fractures in the elderly patients, PCCP has advantages of allowing early weight-bearing after operation, a high rate of fracture union, limited complications and quick and fine functional recovery of the hip. However, it should be used with caution in patients with severe osteoporosis, displaced subcranial or high shear fracture.

Objective To analyze the relationship between serum microRNA(miR)-96-5p,miR-486-5p lev-els and inflammatory response and prognosis in patients with post-traumatic sepsis.Methods A total of 205 trauma patients who were admitted to the First Affiliated Hospital of Hainan Medical University from March 2019 to October 2022 were selected,patients were divided into sepsis group(77 cases)and non-sepsis group(128 cases)according to whether they progressed to sepsis during treatment.The relative expression levels of serum miR-96-5p and miR-486-5p in two groups were detected by real-time fluorescence quantitative PCR,and the levels of serum inflammatory factors[C-reactive protein(CRP),procalcitonin(PCT),interleukin-6(IL-6)]were detected.The correlation between serum miR-96-5p,miR-486-5p expression and inflammatory fac-tors in sepsis group were analyzed by Pearson correlation analysis.The prognosis of patients with post-trau-matic sepsis was further analyzed,patients were divided into the survival group and the death group according to the survival outcome during hospitalization.The influencing factors of death in patients with post-traumatic sepsis were analyzed by multivariate Logistic regression model,the value of miR-96-5p and miR-486-5p for predicting death of patients with post-traumatic sepsis were analyzed by receiver operating characteristic(ROC)curve.Results The relative expression level of serum miR-96-5p in sepsis group was lower than that in non-sepsis group(P<0.05),and the relative expression level of miR-486-5p was higher than that in non-sepsis group(P<0.05).The levels of serum CRP,PCT and IL-6 in sepsis group were higher than those in non-sepsis group(P<0.05).Pearson correlation analysis showed that the relative expression level of miR-96-5p in sepsis group was negatively correlated with the levels of CRP,PCT and IL-6(P<0.05),and the relative expression level of miR-486-5p was positively correlated with the levels of CRP,PCT and IL-6(P<0.05).A-mong 77 patients with post-traumatic sepsis,67 survived and 10 died.Multivariate Logistic regression analysis showed that the increase of the relative expression level of miR-96-5p was a protective factor for death in pa-tients with post-traumatic sepsis(P<0.05),and the increase of injury severity score(ISS)and the relative expression level of miR-486-5p were risk factors for death in patients with post-traumatic sepsis(P<0.05).The ROC curve showed that the area under the curve of the combined detection of ISS,miR-96-5p and miR-486-5p for death in post-traumatic sepsis patients was 0.860,and sensitivity and specificity were 0.900 and 0.821,respectively.The predictive efficacy of combined detection was higher than that of individual detection.Conclu-sion Serum miR-96-5p is lowly expressed and miR-486-5p is highly expressed in patients with post-traumatic sepsis,and the levels of inflammatory factors increase in the body.The expression of miR-96-5p and miR-486-5p is closely related to the inflammatory response,the two are also influencing factors for the death of patients and have certain predictive value for prognosis,and they could provide a reference basis for early clinical evalu-ation of the condition and prognosis.

Objective:To analyze the effect of birth parity on life expectancy (LE) and healthy life expectancy (HLE) among rural women.Method:A total of 15 304 women aged 40 to 79 years who participated in baseline and follow-up surveys were selected from a rural cohort in Henan province. The LE and HLE of women with different birth parity were calculated by using multi-state life table.Results:There were 1 195 (7.8%), 7 782 (50.8%), 3 867 (25.3%) and 2 460 (16.1%) women with 1, 2, 3 and 4 birth parities, respectively, and the M ( Q1 and Q3) of age were 50.3 (47.3, 53.4) and 53.3 (48.8, 60.7), 62.6 (55.4, 66.9) and 69.5 (64.7, 73.4) years old, respectively. LE at 40 years old was 44.5, 44.8, 45.1 and 45.4 years old, and HLE was 17.7, 18.3, 18.8 and 19.3 years old, respectively. LE at age 40 increased by 0.3, 0.6, and 0.9 years in women with 2, 3, and 4 birth parities or more and HLE increased by 0.5, 1.1, and 1.6 years, respectively, compared with women with 1 birth parity. For women with higher and lower socioeconomic status who had 4 birth parities or more, the LE at age 40 was 47.1 and 43.9 years, respectively, an increase of 0.2 and 0.1 years over women with 1 birth parity, respectively; and the HLE was 20.4 and 18.7 years, respectively, an increase of 1.4 and 1.3 years over women with 1 birth parity, respectively. Conclusion:LE and HLE show an upward trend with the increase of birth parity among rural women.

Objective:To analyze the effect of birth parity on life expectancy (LE) and healthy life expectancy (HLE) among rural women.Method:A total of 15 304 women aged 40 to 79 years who participated in baseline and follow-up surveys were selected from a rural cohort in Henan province. The LE and HLE of women with different birth parity were calculated by using multi-state life table.Results:There were 1 195 (7.8%), 7 782 (50.8%), 3 867 (25.3%) and 2 460 (16.1%) women with 1, 2, 3 and 4 birth parities, respectively, and the M ( Q1 and Q3) of age were 50.3 (47.3, 53.4) and 53.3 (48.8, 60.7), 62.6 (55.4, 66.9) and 69.5 (64.7, 73.4) years old, respectively. LE at 40 years old was 44.5, 44.8, 45.1 and 45.4 years old, and HLE was 17.7, 18.3, 18.8 and 19.3 years old, respectively. LE at age 40 increased by 0.3, 0.6, and 0.9 years in women with 2, 3, and 4 birth parities or more and HLE increased by 0.5, 1.1, and 1.6 years, respectively, compared with women with 1 birth parity. For women with higher and lower socioeconomic status who had 4 birth parities or more, the LE at age 40 was 47.1 and 43.9 years, respectively, an increase of 0.2 and 0.1 years over women with 1 birth parity, respectively; and the HLE was 20.4 and 18.7 years, respectively, an increase of 1.4 and 1.3 years over women with 1 birth parity, respectively. Conclusion:LE and HLE show an upward trend with the increase of birth parity among rural women.