Objective To investigate infection statue and antibiotic susceptibility of Mycoplasma urealyticum and Mycoplasma hominis in the genitourinary tract in our area, so as to instruct the rational use of antibiotics. Methods Genitourinary secretions were collected with swabs. They were cultured with the diagnostic kit of Mycoplasma (Biomerieux Company) to detect M. urealyticum and M. hominis. Meanwhile the susceptibility of Mycoplasma against 9 antimicrobial agents was tested with the same kit. According to the manual of the kit,the results were read. The data were statistically analyzed. Results A total of 2 410 samples were collected, and the positive rate was 58. 1%. Among 1410 positive cases of Mycoplasma, 901 cases were M. urealyticum (37.4%), 85 were M. hominis (3.5%), and 415 were M. urealyticum combined with M. hominis (17.2%).The susceptibility rate of M. urealyticum to josamycin, pristinamycin, ciprofloxacin was 98. 8%, 98. 8% and 6. 4% respectively, while the susceptibility rate of M. urealyticum combined with M. horninis was 86.9%,86. 8% and 2.6% respectively. Conclusion M. urealyticum is the major cause of Mycoplasma infection in genitourinary system. Josamycin and pfistinamycin are more effective than other antimicrobial agents to treat Mycoplasma irffection. Ciprofloxacin is more resistant than other antimicrobial agents. Sensitive antibiotics should be selected based on the results of bacterial culture and drug sensitivity tests so as to raise the clinical curative effects.

Background: Enteritis is one of the most frequently reported symptoms in piglets infected with porcine circovirus type 2 (PCV2), but the immunopathogenesis has not been reported. Objectives: This study examined the effect of a PCV2 infection on the intestinal mucosal immune function through morphological observations and immune-related molecular detection. Methods: Morphological changes within the ileum of piglets during a PCV2 infection were observed. The expression of the related-molecules was analyzed using a gene chip. The immunocyte subsets were analyzed by flow cytometry. The secretory immunoglobulin A (SIgA) content was analyzed by enzyme-linked immunosorbent assay. Results: The PCV2 infection caused ileal villus damage, intestinal epithelial cells exfoliation, and an increase in lymphocytes in the lamina propria at 21 days post-infection.Differentially expressed genes occurred in the defense response, inflammatory response, and the complement and coagulation cascade reactions. Most of them were downregulated significantly at the induction site and upregulated at the effector site. The genes associated with SIgA production were downregulated significantly at the induction site. In contrast, the expression of the Toll-like receptor-related genes was upregulated significantly at the effector site. The frequencies of dendritic cells, B cells, and CD8 + T cells were upregulated at the 2 sites. The SIgA content decreased significantly in the ileal mucosa. Conclusions PCV2 infections can cause damage to the ileum that is associated with changes in immune-related gene expression, immune-related cell subsets, and SIgA production.These findings elucidated the molecular changes in the ileum after a PCV2 infection from the perspective of intestinal mucosal immunity, which provides insights into a further study for PCV2-induced enteritis.