Objective To explore the clinical application of treponema pallidum enzyme-linked immunosorbent assay (TP-ELISA),syphilis toluidine red unheated serum regain test (TRUST) and treponema pallidum particle agglutination assay (TPPA) for the diagnosis of syphilis,improvement of the process of sequence syphilis screening program and thus assisting clinical diagnosis and treatment of syphilis.Methods The serum samples were both screened by TP-ELISA and TRUST simultaneously.The positive was confirmed by TPPA test.Syphilis serological test results of patients were collected and retrospectively analyzed.Results The 3 618 cases out of total 82 026 serum samples were reactive by TP-ELISA,453 were reactive to TRUST,and 1 684 were confirmed positive on TPPA;thepositive rate were 4.41%,0.55%and 2.05%;The TP-ELISA,TRUST and TPPA positive conformity rate or titer were rising with the TP-ELISA S/CO and TRUST titer increasing.Conclusion There is a positive correlation of TP-ELISA S/CO among the TPPA,TRUST of positive conformity rate and titer.Using TP-ELISA and TRUST as screening,TPPA as confirmed test,the diagnostic efficiency of the process of sequence syphilis screening program can be improved.

ObjectiveTo investigate the method and efficacy of cholelithotomy by laparoscopy and cholangioscopy, a controversial therapy for cholecystolithiasis. MethodsForty-eight patients with cholecystolithiasis who were admitted to our hospital and treated with gallbladder-preserving cholelithotomy by laparoscopy and cholangioscopy from March 2012 to June 2013 were enrolled as subjects. ResultsAfter surgery, all patients recovered without complications. One patient had relapse of cholecystolithiasis at six months after surgery. One patient had gallbladder atrophy at one year after surgery. ConclusionGallbladder-preserving cholelithotomy by laparoscopy and cholangioscopy is a feasible and safe therapy for cholecystolithiasis. The therapy achieves satisfactory short-term outcomes. However, the long-term efficacy of this therapy needs further investigation.