The incidence of invasive candida infection in critically ill patients is rising year by year. It's clinical manifestation lacks specificity which is easy to result in missed diagnosis or misdiagnosis.Tradi-tional diagnosis methods need very long time and have low positive rate,which are easy to delay treatment,so early diagnosis is particularly important.1 ,3-β-D-glucan,mannan and antibodies,anti-enolase antibodies,can-dida score and colonization index may be helpful to make early diagnosis.

In the treatment of 80 cases of the patients with deficient and cold pattern in the spleen and stomach by external application of herbal paste on the acupoints, the results showed remarkable effect in 52 cases, improvement in 24 cases, failure in 4 cases and the total effective rate in 95.0%. Remarkable therapeutic effects were achieved in three different diseases of same pattern. External application of herbal paste on the acupoints is definitely effective for stomachache in deficient and cold pattern of the spleen and stomach.

ve To explore the air pollutibn by fluoride created by ceramics. Methods Air sampling, data analysis and evaluation were carried out in 8 sampling locations of Cizao town composed mainly by ceramics. Results The daily average levels of fluoride in air showed a range of 0.002 ?0.003 mg/m3 and over-standard rates of 14.29% ~ 100% respectively. The average levels of pollution indexes ranged from 0.81 to 3.59 in Cizao town. Conclusion Because the air had been polluted by fluoride in Cizao town, the environmental protection management and monitoring fluoride in ambient air should be strengthened further.

Objective To investigate the effect of fibroblast growth factor receptor 3(FGFR3) in development phase of murine small intestine.Methods Wild type mice and their littermate FGFR3+ mice were used to observe their morphology and proliferation characteristics of epithelia of small intestinine in development phase and changes of FGFR3 expression.Results Mutant mice had lower density and their villa were lower than those of the controls.But to the depth of crypt,the results were conversely.Cells in proliferation mainly located in intestine crypt.Mutant mice had more proliferation than the controls at every time point.Expression of FGFR3 was detected at birth(day 1),and the expression was maximal from day7 to day 21,decreasing rapidly thereafter to reach the relatively low at day35.And the expression of FGFR3 also located in intestine crypt,overlapping with that of Brdu.Conclusion FGFR3 improves formation of murine intestine crypt in development phase.

Objective:To eliminate the interference of matrix in urea cream on the microbial limit test. Methods: A certain a-mount of magnesium sulfate was added to the test solution followed by stirring and placing sometime to make the cream layering, and the water layer was used for the microbial limit detection. Results:After magnesium sulfate was added to urea cream, microbial limit test was not affected by the matrix. The average recovery of Escherichia oli, Staphylococcus aureus, Bacillus subtilis, Candida albicans and Aspergillus niger was 96. 00%, 97. 00%, 100. 00%, 99. 00% and 101. 00%, respectively. The negative and positive results of Psudomonas aeruginosa and Staphylococcus auress were reliable. Conclusion:In the detection of microbial limit for urea cream, mag-nesium sulfate can be used as a good auxiliary agent to make the detection method more convenient and reliable.

Experimental membranous glomerulonephritis(MGN) in Japanese white rabbits was induced by administration of C-BSA according to Border's method. The results showed that the lipid peroxides(LPO) content in plasma and renal cortex were significantly increased compared with that in the controls. On the other hand, the activities of superoxide dismutase(SOD) and glutathione peroxidase(GSH-px) in whole blood and renal cortex were significantly reduced compared with that in the controls. The results suggested that experimental MGN existed a mechanism of cellular injury manifested as lipid peroxidation in biomembrane, and such injury might be related to toxic effects of reactive oxygen on biomembrane. Tetramethylpyrazine was used to treat experimental MON. The results showed that the drug might have reduced the amount of proteinuria and the extent of lipid peroxidative injury. At the same time, the drug might haod raised the activities of SOD and GSH-px, and improved the lesion ot some extent. It is suggested that tetramethylpyrazine had the effect of anti-lipid peroxidative injury.

On the basis of regional characteristics and in-depth investigations, the hospital the authors work with has devoted major efforts to advancing the discipline of emergency treatment of trauma by closely integrating scientific management with the deepening of reform. The series of measures adopted include: enhancing emergency treatment awareness, purchasing advanced equipment, updating facilities for emergency treatment, establishing an echelon of talented personnel, implementing emergency treatment standards, perfecting emergency treatment procedures, shortening severe trauma patients' time of stay in the emergency treatment room, and performing operations in a timely manner. As a result, marked effects have been achieved.

Myocardial hypertrophy is an independent risk factor for cardiac events. Mitogen-activated protein kinases (MAPK), including extracellular signal-regulated kinases, C-jun N-terminal kinases and P38-MAPK, are the common intracellular pathway of transducing hypertrophic signs. All three MAPK subfamilies play an important role in development of myocardial hypertrophy.

BACKGROUND: Tissue engineering has a promising prospect in corneal transplantation. Scaffolds are always restricting the development of tissue-engineered cornea. OBJECTIVE: To analyze application of different seed cells and scaffolds, and to summarize the progress of tissue-engineered cornea in recent years.METHODS: First author searched literature from CNKI (2000/2010-10) and PubMed database (2000/2010-10). The key words are tissue engineering, corneal transplantation in Chinese or English. A total of 223 literatures were seized by computers, according to the inclusion criteria, papers concerning the advance, application and reconstruction of tissue-engineered cornea were analyzed. Finally, 33 papers were included for further analysis. The present study was to analyze the seed cells, scaffolds, organ building and clinical applications of tissue-engineered cornea and investigate the development direction in the future. RESULTS AND CONCLUSION: The results show that seed cells and scaffolds are the focus of the studies now. Corneal transplantation has a high success rate in organ transplantation since the immune privilege of eye and avascular cornea, and it will be able to be a tissue engineering organ that can be largely built, easy to transplant. Reconstruction of cornea has reached first base, but every kind of scaffold has certain drawbacks, so the next goal is to find an ideal scaffold material.