Objective To observe the clinical effects of treating LIDP with needle-knife therapy plus balance.acupuncture therapy.Methods 120 LIDT patients were randomly recruited into a treatment group and a control group.with 60 patients in each group.The treatment group was treated with needle-knife therapy plus balance-acupuncture therapy andthe control group was treated with needle-knife therapy exclusively.The therapeutic effects were evaluated after thetreatment.Resuits There was significant difference between the two groups in terms of recovery rateandthe course of treatment. Conclusion Needle-knife therapy plus balance-acupuncture therapy was obvious better than exclusive needle-knife therapy in treating LIDT in terms of recovery rate and the course of treatment.

BACKGROUND: As a specially differentiated central nerve, optic nerve is once injured, retinal neurons are hard to restore and regenerate. As a result, visual function is lost permanently. Currently, an effective clinical treatment is not available. Due to the multi-directional differentiated potential of stem cells, their role in optic nerve protection and restoration following injury is becoming a hot research spot.OBJECTIVE: To review the classification and transplantation of stem cells in optic nerve protection study, as well as roles of stem cells transplantation in optic nerve protection and repair following injury.METHODS: The database of ISI Web of Knowledge platform was searched on computer from February 1994 to April 2009, with the key words "stem cells, optic nerve" in English. In the same way, the database of CNKI from February 1994 to April 2009 was also screened, with the key words "stem cells, optic nerve" in Chinese.RESULTS AND CONCLUSION: A total of 98 literatures related to stem cells in optic nerve protection and restoration after injury are collected, in which 23 papers are Chinese and 75 papers are English. The studies published earlier, repetitively and similarly are removed, and 38 papers which comply with the standards are involved into this review. Stem cells own the potential of self-renew physiologically, as well as potential of repairing the injuries. At present, most of the researches on stem cells in optic nerve protection and restoration after injury are represented in morphosis. How to implant stem cells safely and effectively and how to recover stable visual function still need our efforts. Stem cells derived from skin, peripheral blood, fat and other tissues are convenient to draw materials and are commendable subjects, we should study them deeply to make preferable use.

Objective: To exploration the self-efficacy, self-esteem, and positive/negative affect and the re-lationships among them in Chinese adolescents. Methods: The Rosenberg's Self-Esteem Scale, Generalized Self-Ef-ficacy Scale, and Adolescents Positive/Negative Affect Scale were administrated to 2247 middle school students sampled from 16 schools from 6 districts. Results: Self-esteem tended to increase as grade [(2.91±0.38), (2.93±0.41), (3.01±0.41), (2.98±0.44), (3.04±0.41), (3.17±0.42)]. Boys had higher self-efficacy [(2.74±0.39)vs.(2.68±0.40), P <0.001]. Two senior grades had higher self-efficacy than other 4 grades (P<0.05). Girls had higher positive and negative affect [(2.77±0.67)vs.(2.68±0.67), (2.22±0.70) vs.(2.13±0.68), Ps <0.05]. Junior grade one had the highest positive effect (2.85±0.62), grade one and two in high school had more negative effect than other 4 grades (2.26±0.71, 2.25±0.73).Self-efficacy and self-esteem were positively correlated with positive affect (r=0.24, 0.36), negatively correlated with negative af-fect (r=-0.17, -0.32) ; self-esteem significantly mediated the relationship between self-efficacy and positive/negative affect (Z=13.00, -13.07) . Conclusion: Self-esteem tends to increase as grade, boys have higher self-efficacy than girls, and girls have higher positive and negative affect than boys. Self-esteem mediates the rela-tionship between generalized self-efficacy and positive/negative affect in Chinese adolescents after controlling the effects of gender and grade.

Myoblast transplantation has been carried out for a certain period,but its efficiency in repairing inherited myopathy or severe muscular injury is not satisfactory.The improvements in myoblast culture,transplantation approach,recipient and cell preparation before transplantation,scaffold material and its selection,and body immunity can positively influence transplantation efficacy,and improve recipient function.In addition,these improvements influence the prospect of myoblast transplantation or cell-mediated gene therapy.

Objective　To better understand the clinical characters of juvenile onset spondyloarthropa-thies (JSpA).Methods　The clinical and laboratory data of 190 in-patients with JSpA were analyzed and the diagnosis,classification and differentiation of juvenile onset arthritis were discussed.Results　Among these 190 patients,163 were male,with a male to female ratio 6∶1.Of them 92.1% had the disease after the age of 8.Peak of age at onset was 12 to 15 years;157(82.6%) patients had peripheral arthritis and only 23(12.1%) patients felt low back pain at onset.During the disease course, peripheral arthritis was found in 187(98.4%) patients and the history of low back pain or buttock pain was recorded in 123(64.7%).The interval between peripheral arthritis and low back pain was from 0 to 20 years,with an average of (3.2±4.5)years.Extra-articular features including enthesitis in 67(35.3%)patients,dactylitis in 20(10.5%),iritis in 9(4.7%) were observed.HLA-B27 was positive in 87.9%(160/182) patients.Sacroiliitis on X-ray was observed in 76.0%(136/179) patients,and 106(55.8%) patients were diagnosed juvenile ankylosing spondylitis (JAS) according to 1984 New York modified criteria.The average disease course in JAS was (6.3±6.2) years,longer than that in JSpA (P＜0.01).Conclusion　The concept of JSpA is helpful to early diagnosis and treatment of juvenile onset arthritis.The JSpA are characterized by asymmetric lower limb predominant oligoarthritis,a wide spectrum of extra-articular features,presence of HLA-B27 and familial history of SpA or psoriasis.It will take an average of 6.3 years for JSpA patients to fulfill the diagnostic criteria of adult AS.

Anti-retroviral (ARV) drugs have been successfully used for HIV/AIDS therapy,and their uses in pre-exposure prophylaxis (PrEP) for HIV infection is becoming increasingly important,and is drawing more attentions.Reports on seven large scale,multicentric clinical trials provide significant clinical data for further study and implementation of PrEP.This article reviews the current situation,necessity,questions,problems and prospects of PrEP for HIV infection.

Liver functional reserve which functioned as the major evaluation factor for the tolerance of the liver surgery or the liver trauma had already been used wildly in preoperative evaluation for liver surgery.So far there were plenty of techniques for liver functional reserve.But each technique had its own advantages and disadvantages.This article briefly discussed the most wildly used techniques for liver functional reserve in preoperative evaluation.

Objective To study the role of 1,4,5-trisphosphate inositol(IP3) and bax gene expression in inhibiting transplanted hepatocellular carcinoma of nude mice by quercetin.Methods After animals with hepatocellular carcinoma were treated with quercetin 1mg/kg/d(ip)for 3 weeks,the volume and weight of tumor was measured,and IP3,Bax mRNA,and Bax protein were assayed by IP3- Birtrak assay,RT-PCR,and Western blotting,respectively;and compard with coutrol group.Results The tumor volume and weight of animals treated with quercetin were lower than those of control[(15.8?10.1) mm3 vs.(52.3?26.5 mm3;(44.8?10.4) mg vs.(91.3?31.4) mg],IP3 content was lower than that of control[(15.9?2.8)pmol/mg protein vs(35.3?6.6)pmol/mg protein],Bax mRNA expression was not significantly different between the 2 groups[RI which was the gray degree multiply area of bax / the gray degree multiply area of ?-actin(0.64?0.12) vs.(0.56?0.15)],Bax mRNA expression was higher in group treated with quercetin than that of control[(3.16?0.95) vs.(1.37?0.48)].Conclusions Quercetin can inhibit growth of transplanted hepatocellular carcinoma of nude mice by reducing IP3 production and bax protein expression.

Objective:To investigate the expression of focal adhesion kinase(FAK) and Ki67 in the invasive breast ductal carcinoma tissues, seeking their relationship with tumor invasion and metastasis and to study the mechanisms for cell signaling transduction mediated by FAK in the carcinogenesis and progress of breast carcinoma.Methods:Tissues were obtained from 88 patients with invasive breast ductal carcinoma and 25 with breast ductal epithelial benign proliferative disease. The expression of FAK and Ki67 was examined by immunohistochemical technique(streptavidin peroxidase conjugate method, SP).Results:In 88 cases of invasive breast ductal carcinoma, the positive incidences of FAK was 68.2%(60/88), and the Ki67 proliferation index was 29.6%?22.9%, which distinguished apparently to those of the control group(P0.05). In breast carcinoma tissues, the expression of FAK was positively related to Ki67 proliferation index(P

OBJECTIVE:To discuss the social factors influencing therapeutic effects of drugs.METHODS:The relevant literature were reviewed and collected to analyse the causes of social factors in influencing therapeutic effects of drugs.RESUL_ TS&CONCLUSION:The efficient amse in affecting actions of drugs is abuse of drugs.In order to avoid the abuse of drugs,we should strengthen the management of drug circulation,establish mechanisms of fair competition and management of OTC,in supervising clinical physicians and guarding against the abuse of drugs.