Objective To control study the treatment effect of functional dyspepsia of stagnation of liver-qi type treated by liver-coursing depression-resolving decoction added psychological counseling.Methods130 patients with functional dyspepsia of liver-qi stagnation type who were treated in the inpatients and outpatients in our hospital from January 2015 to January 2016 were selected, to randomLy grouping.The 65 cases of the control group was given the conventional therapy, and the another 65 cases of the treatment group was given liver-coursing depression-resolving decoction added psychological counseling at the the same term based on the control.After the continue treatment for 4 weeks, to compared the clinical curative effect of them.ResultsThe treatment effective rate of the treatment group was 94%, he treatment effective rate of the control group was 78%, there was statistical significance (P<0.05).To compared with the grade of traditional Chinese medicine symptom (distending pain in the stomach, satiation, belching, inhibited defecation) and discovered that the grades of them were lower after treatment, and there has the significant improvements that compared with that before the treatment, there was statistical significance (P<0.05), and the treatment group was significantly lower than the control group, there was statistical significance (P<0.05).The Hp Infection and the bile regurgitation of them after treatment was significantly improved, the treatment group was significantly better than the control group, there was statistical significance (P<0.05), the treatment group was significantly better than the control, there was statistical significance (P<0.05).To compared the 71% of the negative conversion ratio of Hp urease detection of the treatment group that was significantly greater than the 40% of the control group after detection, there was statistical significance (P<0.05).The gastric emptying time of them was significant improved after treatment, and compared with that before the treatment, there was statistical significance (P<0.05).For the gastric emptying time, to compared with the control group, the improvement effect of the treatment group was outstanding, there was statistical significance (P<0.05).After treatment, the grade of anxiety and depression was significantly decreased of them, and to compared with the control group after treatment, the grade of anxiety and depression of the treatment group was significantly improved, there was statistical significance (P<0.05).There was no obvious untoward effects of them, there was no statistical significance.ConclusionThat has an exact effect of functional dyspepsia of stagnation of liver-qi type treated by liver-coursing depression-resolving decoction added psychological counseling, to provide an effective way for the clinic treatment of the functional dyspepsia (stagnation of liver-qi type).Meanwhile, to provide the new theoretical foundation for the traditional chinese medicine therapy of functional dyspepsia.

OBJECTIVE:To establish a method for the determination of related substances in Vildagliptin tablets. METHODS:HPLC method was adopted. The determination was performed on Xterra MS C18 column with mobile phase consisted of [phosphate buffer-water-acetonitrie-methanol(400 : 600 : 15 : 15,V/V/V/V)]-[phosphate buffer-acetonitrile-methanol(400 : 450 : 150,V/V/V)](gra-dient elution)at the flow rate of 1.2 mL/min. The detection wavelength was set at 210 nm and column temperature was 35 ℃. The sample size was 10 μL. RESULTS:The linear ranges were 18.80-188.0 μg/mL for impurity A(r=0.9995),22.64-226.4 μg/mL for impurity B(r=0.9996),21.74-217.4 μg/mL for impurity C(r=0.9997),19.12-191.2 μg/mL for impurity D(r=0.9998). The limits of detection were 4.18,2.68,1.12,1.34 μg/mL,respectively;RSDs of precision,stability and reproducibility tests were lower than 3%;the recoveries of impurity A,B,C and D were 97.9%-103.1%(RSD=2.01%,n=9),98.8%-104.1%(RSD=1.93%,n=9),98.0%-103.6%(RSD=1.81%,n=9),100.8%-104.1%(RSD=0.98%,n=9),respectively. CONCLU-SIONS:The method is simple and accurate,and can be used for the determination of related substances in Vildagliptin tablets.

Objective To explore the curative effect of anterior and posterior wall of the uterus through suture combined with intrauterine balloon compression and uterine artery ascending ligation to treat the hemorrhage of the placenta.Methods In June 2015 to June 2017 in our hospital 60 cases of placenta previa during cesarean section hemorrhage patients, were randomly divided into treatment group and control group , the control of intrauterine balloon compression and ascending branch of uterine artery using the group of patients, patients in the treatment group in the control group based method on the back wall of the lower uterine segment through suture in the treatment of. The curative effect and operation of the two groups were compared.Results The total effective rate was higher in the treatment group than the control group(P<0.05). The intraoperative blood loss, postoperative 24h blood loss of the treatment group patients were lower than the control group, the treatment group patients with operation time, length of hospital stay were lower than the control group(P<0.05).Conclusion The lower uterine segment through anterior and posterior wall suture combined with intrauterine balloon compression and ascending branch of uterine artery ligation is an effective method in treatment of hemorrhage in cesarean section for placenta previa, facilitate clinical application.

OBJECTIVE To introduce and discuss the quality management system of medical loaner instrumentation and implantables in the US.METHODS With US national standards and industry guidelines on loaner instrumentation and implantables,and the practical problems we encountered in China on this subject,we will discuss their quality management.RESULTS We should apply whole process quality management to loaner instrumentation and implantables.CONCLUSIONS Zero tolerance quality management in loaner instrumentation and implantables is crucial for medical safety.

L1 cell adhesion molecular is a type Ⅰ membrane glycoprotein of immunoglobulin superfamily. L1 is expressed mainly in nervous system and plays important roles in nervous system development, learning and memory. L1 cytoplasmic domain (L1CD) is important for L1 function by mediating signaling. To investigate the molecular mechanisms of signaling mediated by L1CD, yeast two-hybrid assay was carried out to screen the human brain cDNA library using L1CD as the bait. After sequence analysis and BLAST, several candidates were identified. One candidate is PAX6 transcription factor. L1CD and PAX6 cDNA were cloned in expression vectors and cotransfected into COS-7 cells. The interaction between L1CD and PAX6 was verified by co-immunoprecipitation assay. These preliminary results suggest that L1CD may be involved in transcription regulation.

OBJECTIVE To evaluate the changes in serum levels of TNF-? and IL-6 after thoracic operation,and the relationship with multiple organ dysfunction syndrome(MODS).METHODS Serum levels of TNF-? and IL-6 in 46 cases of benign disease between 2000 to 2003 were observed on the day of operation,the 3rd,7th,14th and 21th days after operation,the patients were divided into two groups according to the presence of MODS.RESULTS Cases occurred MODS in two weeks after thoracic operation,the serum levels of TNF-? and IL-6 were markedly higher in MODS than that in non-MODS,the correlation coefficients between them were r=0.9145,P

Objective:To establish a method for the determination of the related substances in ticagrelor tablets. Methods:An op-timal HPLC method was set up and a Hypersil BDS C18 column (200 mm × 4. 6 mm, 5μm) was adopted. The mobile phase A was wa-ter (pH was adjusted to 3. 0 by phosphoric acid) and the mobile phase B was acetonitrile with gradient elution at a flow rate of 1. 0 ml ·min-1 , and the detection wavelength was set at 255 nm. The column temperature was 30 ℃, and the volume of injection was 5 μl. Results:The resolutions between ticagrelor and the adjacent impurity ( impurity E) , and between ticagrelor and the known impurities were all greater than 1. 5. Impurity A-H all had a good linear relationship within the range of 2. 0-20. 0 μg·ml-1(r>0. 996 0). The average recovery was 99. 8%, 98. 1%, 96. 5%, 101. 2%, 99. 3%, 100. 7%, 102. 1% and 103. 4% with RSD of 1. 02%, 0. 88%, 1. 13%, 0. 56%, 0. 79%, 1. 11%, 0. 63% and 1. 39%, respectively(n=9). Conclusion:The method is reproducible and accurate in the determination of the related substances in ticagrelor tables.

Objective To explore the value of combined detection of carbohydrate antigen 125(CA125),human epididymis secre-tory protein 4 (HE4)and plasma D-dimer in early diagnosis of ovarian cancer.Methods Fifty-six patients with ovarian cancer and 50 patients with benign ovarian tumors in the gynecology department of our hospital from January 2014 to June 2016 were selected. Contemporaneous 50 women undergoing healthy physical examination were selected.The chemiluminescence method was adopted to determine serum CA125 and HE4 levels,and the immune turbidimetry method was adopted to detect plasma D-dimmer level.Their early diagnostic values for ovarian cancer were comparatively analyzed.Results The serum CA125 and HE4 levels and plasma D-di-mer level in the cancer group were significantly higher than those in the control group with statistical difference(P<0.05),moreo-ver HE4 had higher sensitivity(89.28%)and higher specificity (88.00%)for detecting ovarian cancer.In the 3-index combined de-tection,sensitivity and specificity were greatly improved.Conclusion The combined detection of CA125,HE4 and plasma D- dimer can improve the accuracy of early diagnosis of ovarian cancer.

Objective To analyze the different procedures and their clinical effects on treatment for pan-creatic pseudocyst( PPC) .Methods The management and outcome of 78 patients with PPC from Jan.2002 to Jun.2012 were retrospectively analyzed.Results 20 patients underwent conservative treatment and the pseudo-cysts absorbed spontaneously during follow up.Invasive procedures were performed in 58 cases, including percu-taneous catheter drainage in 3 cases with 1 postoperative pancreatic leakage and 1 postoperative pancreatic ab-scess;PPC external drainage in 5 cases with 2 postoperative pancreatic leakage and 1 postoperative bleeding;pancreatic pseudocyst stomach anastomosis in 15 cases with 1 postoperative gastrointestinal bleeding;Roux-en-Y cystjejunostomy in 29 cases with 2 gastrointestinal bleeding;cystduodenostomy in 2 cases;pancreatic pseudocyst excision in 2 cases with 1 postoperative pancreatic leakage; and laparoscopic cystogastrostomy in 2 cases.Con-clusions At present, there are various treatment options for pancreatic pseudocyst and it is necessary to choose treatment strategy according to patient's condition.Internal drainage is still the primary option of surgical proce-dures in treatment for pancreatic pseudocyst.

Objective To explore the relationship between pituitary tumor transforming gene(PTIG) and Ki67 expression and their significance in multiple adolescent breast fibroadenomas (fibroadenomatosis)and single persons(fibroadenomas) and their clinical value.Methods A total of 53 fibroadenomatosis and 160 fibroadenomas specimens in our hospital were collected.All the samples were fixed with 10％ formalin and then embedded with paraffin.The protein expression of PTTG and Ki67 was detected by immunohistochemical staining.The relation between expression of PTTG and Ki67 in fibroadenomatosis was analyzed.Results The positive rate of PTTG and Ki67 was higher in fibroadenomatosis than in fibroadenomas (P ＜ 0.005).Conclusions High expression of PTTG and Ki67 in fibroadenomatosis leads to the proliferation of tumor cell and upregulates tumor angiogenesis.High expression of PTTG and Ki67 may play important roles in tumorigenesis and pathological process of fibroadenomatosis.