China ; Hearing Tests ; Humans ; Infant, Newborn ; Neonatal Screening

Objective To evaluate the diagnostic value of myocardial enzymes on the cardiac muscle injury for the children with acute mycoplasma pneumonia(MP).Method The levels of phosphocreatine kinase-MB(CK-MB),lactate dehydrogenase(LDH),aspartate aminotransferase(AST)in 48children with MP(MP group)were measured,48 normal children served as control group.Results The levels of AST,LDH,CK-MB were(37.18±6.15),(294.10±46.20),(84.50±13.70)U/L in acute stage in MP group,they were higher than those in control group[(25.14±10.50),(140.50±76.21),(19.50±4.10)U/L](P＜0.01).All the values above was descended in recovery phase in MP group,but the values of CK-MB[(27.80±8.40)U/L]and LDH[(189.50±69.73)U/L]were still higher than those in control group(P＜0.01 or＜0.05).Conclusions Children with MP may combine myocardial injuries and CK-MB isa parameter to diagnose the damage.Protection therapy for cardiac muscle should be performed on the children with MP.

Objective To clarify the morphological features, types, regional distributions and cell densities of gastrointestinal (GI) endocrine cells in various parts of the digestive track (DT) of Eumeces elegans. Methods Using immunohistochemical techniques of streptavidin-peroxidase(S-P) method. Results The endocrine cells found in the DT were round or oval, spindle, shuttle, pyramid, flask and bacilliform in shape. Three types of endocrine cells, namely 5-hydroxytryptamine (5-HT) positive cells, somatostatin (SS) positive cells and gastrin (GAS) positive cells were found. No substance P(SP)-, glucagon (GLU)-, pancreatic polypeptide ( PP)- and insulin(INS) positive cells were detected. 5-HT positive cells distributed throughout the whole DT at various densities and they were most predominant cell types in the DT. SS positive cells were detected only in stomach. GAS positive cells showed a restricted distribution and were only demonstrated in the pylorus and duodenum. In the DT of E. elegans the region with the highest degree of cell type heterogeneity was pylorus and all types of endocrine cell along the DT showed peak density in the pylorus as well. Conclusion Some common features of the distribution of different types of GI endocrine cells are found between E. elegans and other reptiles. This common trait may reflect the similarity in digestive physiology of various vertebrates. On the other hand, some species-dependent unique distribution features of endocrine cells in DT were also detected in E. elegans.

The analysis of 2013 circulating foreign and Chinese books in Library of Capital Medical University showed that the number of foreign and Chinese books classified into R Medicine and Healthand I Literaturewas the largest ,which thus provided the evidence for book acquisition ,book circulation and library holding develop-ment.Certain suggestions were put forward for improving the utilization of books, such as enforcing the acquisition and catalogue , and recommending books that should be bought .

BACKGROUND:Bone morphogenetic protein 9 is proved to promote the osteogenic differentiation of various kinds of stem cel s, but whether it can induce the osteogenic differentiation of dental fol icle cel s in vitro is yet unclear. OBJECTIVE:To investigate whether bone morphogenetic protein 9 can induce the osteogenic differentiation of rat dental fol icle cel s in vitro. METHODS:Purified rat dental fol icle cel s at passage 3 were transfected with bone morphogenetic protein 9 adenovirus. Then, alkaline phosphatase activity, calcium deposition and expression of osteogenesis-related factors at mRNA and protein levels were detected in the dental fol icle cel s. RESULTS AND CONCLUSION:After transfection with bone morphogenetic protein 9, the dental fol icle cel s showed continuously enhanced alkaline phosphatase activities and obviously enhanced calcium deposition. Real-time PCR results demonstrated that the mRNA expressions of alkaline phosphatase, osteocalcin, bone sialoprotein, osteopontin and core binding factor were increased significantly. The western blot assay showed that the expression of osteopontin enhanced in the dental fol icle cel s after transfection with bone morphogenetic protein 9. In summary, bone morphogenetic protein 9 can induce the osteogenic differentiation of dental fol icle cel s.

Sepsis is still a major cause of death among neonates and its morbidity rate remain high nowadays.Because of the atypical symptoms and extremely dangerous progress in neonatal sepsis,early diagnosis and treatment are requisite.But the unique biomarkers are lacking meanwhile.Recent study shows that PBEF influences individual susceptibility,severity and outcome in sepsis.This review synthesizes the research of PBEF in neonatal sepsis in order to provide the evidence of reliable biomarker to diagnose and treat neonatal sepsis in the early stage.

Based on reviewing briefly the development of the general education both in China and United States,selecting respectively a university as the example,the article has analyzed comparatively curriculum programs of general education between the two schools.Aimed at existing problem,from curriculum content carding,structure optimizing,teaching operating and evaluation of the effect,the article has proposed new thinking of reform on the general education of higher institutions.

Breast calciifcation plays an important role in breast cancer in various areas, such as early-stage diagnosis, recurrence monitoring and prognosis prediction. However, the mechanism of breast calciifcations remains elusive. One proposed mechanism is based on an imbalance between the enhancers and inhibitors of physiological mineralization. Traditionally, the classiifcation of breast calciifcation is based on the assessment of morphology and distribution. These radiology features are closely related to pathology and biological behavior of tumor. However, chemical composition of calciifcations can provide us with more information about tumor status. Calciifcations could be divided into two categories according to chemical composition. Type I (calcium oxalate) is found in benign breast disease, while typeⅡ (hydroxyapatite) is found in proliferative lesions, mostly to be associated with malignancy. Moreover, carbonate concentrations in typeⅡ calciifcations is related to pathology grades. Despite emerging imaging technologies such as digital breast tomosynthesis, they are unable to yield any information on chemical composition of breast calciifcations and thus cannot provide a deifnitive marker for classifying benign and malignant lesions. Recent researches show that using Raman spectroscopy, the chemical information of calcification could be obtained non-invasively and might provide us with a better mode for calciifcation diagnosis in the future.

Objective To evaluate the prophylactic use of mitomycin-C to inhibit haze formation after photorefractive keratectomy(PRK).Methods Both a computer-aided search of Medline,OVID,CNKI,Wanfang data and an intensive search by hand were conducted to identify all randomized controlled trials assessing the effect of intraoperative using mitomycinC during PRK.Results Two studies were finally included.The Q test of homogeneity showed no difference between them(?~2=0.17,df=1,P=0.68).The OR value of the studies were 0.17[95% CI 0.05-0.53] and 0.10[95% CI 0.01-0.85],respectively;the pooled OR was 0.14 [95% CI 0.05-0.40],significant difference was found between experimental group and control group(Z=3.75,P=0.000 2).Conclusion Topical intraoperative application of 0.02% mitomycin-C can reduce haze formation in patients with highly near-sightedness after PRK.

Objective To investigate the safety and therapeutic effect of modified laparoscopic Dixon surgery for mid-low rectal carcinoma. Methods From September 2004 to April 2007,26 patients with mid-low rectal carcinoma were selected to receive modified laparoscopic Dixon surgery.Through the anus,the rectum was pulled out,the tumor was removed,and then colon-rectum anastomosis was performed.Results All the operation was completed successfully.The average operation time was(166.3 ? 48.1) min,and the mean blood loss was(235.4 ? 124.7) ml.No patient had urethral injury,dysuria,anastomotic leakage,or other complications after the operation.The patients expelled gas(2.9?0.7) days after the operation.Their mean hospital stay was(8.8?1.4) days.During a follow-up of 15.7 months(6 to 27 months),3 patients had local recurrence,5 patients showed increased frequency of stool,and 18 had satisfied anal function.Conclusion The short-term results indicate that the modified laparoscopic Dixon surgery is safe and effective for mid-low rectal carcinoma,and is associated with minimal invasion.