0.05). Conclusions The LTs synthesis is elevated in RSV bronchiolitis and LTs may participate in the pathogenesis of RSV bronchiolitis. Leukotriene receptor antagonist may play an important role in RSV bronchiolitis. However there still needs further research on the relationship between LTs and eosinophils count in the pathogenesis of RSV bronchiolitis.

Objective To observe the effect of self mading Tongfengxiao decoction in treatment of acute gouty arthritis. Methods 69 patients were randomly divided into two groups. In the control group,33 patients were given diclofenac sodium sustained-release capsules and allopurinol treatment;In treatment group ,36 patients were given to the self mading Tongfengxiao decoction in addition to the western medicine received by the control group. The two groups were all treated for 14 days. Results The total effective rate was 94.4% for treatment group and 87.8% for control group respectively. Therefore, the difference of treatment effect between twp groups was significant ( P ＜0. 05 ) ;Also, the treatment group performed better than control group in term of reducing serum uric acid( P ＜ 0. 05).Conclusion The treatment of Tongfengxiao decoction combined western medicine for acute gouty arthritis had a good synergistic effect, and could reduce serum uric acid and improve clinical symptoms.

Objective To characterize clinical features,antimicrobial susceptibility and the outcome of nosocomial Acinetobacter baumannii meningitis.Methods All patients with nosocomial meningitis due to Acinetobacter baumannii in 2nd Affiliated Hospital Medical School of Zhejiang University between January 2010 and October 2011 were retrospectively reviewed.Results During the study period,40 patients of this nosocomial infection were identified,who came from neurosurgery ward (19 cases,47.5％),neurosurgieal intensive care unit (18 cases,45.0％),emergency intensive care unit (2 cases,5.0％) or intensive care unit (1 case,2.5％).All the patients had a history of recent neurosurgical procedures. Fever and disturbance of consciousness were the major manifestations,and cerebrospinal fluid examination showed elevated white blood ceil count and protein,and reduced glucose.All isolated pathogens were resistant to the first line antibiotics.The fatality rate was high. Conclusions The most common risk factor for nosocomial Acinetobacter baumannii meningitis is neurosurgery. Resistance to the first line antibiotics is common among all pathogens isolated.The prognosis of the meningitis is poor.

Cotton (Gossypium hirsutum) is one of the most important economic crops in the world. Its growth and productivity were affected by environment stresses such as drought, cold and high salinity. Thus, the enhanced stress tolerance in this plant is of great importance. As the dehydration responsive element (DRE) binding protein (DBP) plays an important role in the regulation of plant resistance to environmental stresses and is quite useful for generating transgenic plants tolerant to these stresses, isolation and functional analysis of DBPs in cotton are important to cotton production. In the previous work, a DBP gene from cotton, named as GhDBP1, was isolated and its expression patterns in cotton plants was demonstrated at the transcriptional level. Here, the expression,purification and DNA binding activity of GhDBP1 were reported. The entire coding region of the GhDBP1 gene was inserted into an expression vector, pET28a, and transformed into Escherichia coli BL21 (DE3). The fusion protein was successfully expressed under IPTG induction and the purified recombinant protein was obtained by Ni-NTA affinity chromatography. Non-radioactive electrophoretic mobility shift assay revealed that the purified GhDBP1 protein was able to form a specific complex with the previously characterized DRE element. In addition, the computer modeling of the DNA-binding domain of GhDBP1 were performed using SWISS-MODEL software. The main-chain structures and the folding patterns of the DNA-binding domain of GhDBP1 were similar to the known structure of the DNA-binding domain of the Arabidopsis thaliana GCC box-binding protein AtERF1. These results indicate that GhDBP1 is a DRE-binding transcription factor and might use the structure similar to that of AtERF1 to interact with DRE sequence.

We established a sensitive method for the detection of lead ions in aqueous solution based on the surface energy transfer between gold nanoparticles and fluorescent dyes. The fluorescein-modified thrombin aptamer (5′-FAM-GGT TGG TGT GGT TGG-3′) can be selectively transformed to G-quadruplex induced by lead ions. The conformation changes of the aptamer can alter the distance between the energy donor of fluorescent dyes and the energy receptor of gold nanoparticles, and further enhance the fluorescence intensity. The fluorescence recovery efficiency ( F/F0 ) is proportional to the concentration of lead ions in the range of 12. 5-100 nmol/L following the linear regression equation of y=0. 910﹢0. 007c(R2=0. 997)and the limit of determination is about 10 nmol/L. The proposed method has been applied to the determination of lead ions in tap water with satisfied results.

Objective: To develop an HPLC method for determination of Isocorydine in Jinshining Eye gelatin. Method: AgiLentTC-C18 chromatographi coLumn(4.6 min×150 mm,5 μm) was used,methanoL-0.2% phosphoric acid soLution (adjust the pH 7.0 take methyL aLcohoL)(54:46)as the mobiLe phase, the detection waveLength was 267 nm,flow rate at 1.0 mL· min-1. Result: The calibration curve of Isocorydine as linear between 0.123 0～1.107 0μg, The average reeovery and the relative standard deviation were 97.11% and 1.65%. Conclusions: The method is simple, accurate, repeatable. It can be used for quality control of JinshiLe Eye gelatin.

BACKGROUND:K-wire and steel wire tension band wiring is the most commonly method for the treatment of patel ar fracture. However, there have been many reports of complications related to the mental implants. Based on the reasons above, some scholars begin to replace the steel wire with braided polyester sutures for the treatment of patel ar fracture. OBJECTIVE:To observe the effect of patel ar ring pins and FiberWire braided polyblend sutures tension band fixation for treatment of patel ar fracture. METHODS:We performed a retrospective analysis involving 26 patients with patel ar fracture (16 males, 10 females, at the age of 36-54 years with a mean age of 44.6 years) who were treated with patel ar ring pins and FiberWire braided polyblend sutures tension band fixation. RESULTS AND CONCLUSION:Al the patients were fol owed-up for 6-18 months, average of 12 months. Al patients were primary healing and achieved bone union in 8-16 weeks, averaged in 12 weeks from surgery. There was no fracture fragment displacement, internal fixation loosening and break. No pins protruding from the subcutaneous and irritate the skin. According to the Lysholm&Gil quist scales, 18 cases were excellent, four cases were good, and four cases were fair, with the excellent and good rate of 85%. Tension band fixation using patel ar ring pins and FiberWire braided polyblend sutures resulted in good outcomes with few complications.

In the tumor microenvironment, inflammatory cells regulate intracellular signaling transduction mediated by the corresponding nuclear transcription factor by releasing inflammatory factors ,which leads to transformation of epithelial cells to mesenchymalcellsand eventually promote tumor metastasis. Because of the intimate relationship between inflammation and tumor metastasis , it would be helpful for further enhancement of effectiveness of tumor therapy if we could better understand both the biological character of tumor and the related inflammatory factors.

Objective To establish a SPE-HPLC method for the determination of carbaryl and atrazine in water.Methods Carbaryl and atrazine in water were extracted with Oasis HLB and were eluted with CH3OH,CH3OH-H2O(55:45) was applied as mobile phase,the flow rate was 0.8 ml/min,detection wavelength was 220 nm,column was Symmetry Shield RP18(150 mm ? 3.9 mm,5 ?m),carbaryl and atrazine in water were determined by PDA detector.Results The linear range of the method was 0.05-10.0 mg/L(y=316 263x-350 9,r=0.999 9,for carbary;y=140 151x-258 6,r=0.999 9,for atrazine),The lowest detected concentration was 0.0002 mg/L for carbary and 0.000 5 mg/L for atrazine,respectively(S/N≥3).The recovery rates were 89.6%-97.0%,and the relative standard deviation was 4.0%-6.7%.Conclusion The method was sensitive,accurate and repeatable,which was applicable to the determination of carbaryl and atrazine in water.

The formation of carcinomas of liver, lung and kidney in rats by feeding aminopyrine (AP) plus sodium nitrite (Na NO2) in diet was investigated. 72 young Wister rats were divided into A,B,C,D group. Group A was set as control receiving basal diet and tap water. Another 3 groups were given basal diet containing AP plus Na NO2 (2g/kg each). However, group B was given tap water, group C ascorbic acid solution, group D sea buckthorn (Hippophae rhamnoides L.) juice for drinking. After 38 week's feeding, group B developed tumors in the livers (17/17), lungs (6/17) and kidneys (4/17) and the average life-span was 195 days, group D delayed the development of tumors and the average life-span was 270 days, significantly longer than that of group B (195 days, p