Blood Circulation ; Carbon Dioxide ; blood ; Humans ; Medicine, Chinese Traditional ; Meridians ; Qi ; Science

The network teaching reformation is a result of the rapid development of internet technology and the creation of constructivism theory.The Webquest form practice of network teaching depends on the careful analysis of network teaching aim,the reasonable design of network teaching content,and the scientific arrangement of network teaching plate.The application of the Webquest form is a useful attempt.It is helpful to improve the comprehensive qualities of medical students.And it is helpful to re-construct the relationship between the teacher and the student.

Objective Observe the efficacy of the Qudu-lotion for postoperative edema, postoperative analgesia and promoting wound repair. Methods treatment group(60 patients)applying fumigation and washing with the Qudu-lotion twice a day; control group(60 patients)applying fumigation and washing with 1/5000 potassium permanganate solution. Result The efficacy for the edema and pain of treatment group was superior to those of control group. The cure time was(13.54±3.75)days in treatment group and (17.2±4.22) days in control group. Conclusion In the treatment of postoperative edema, postoperative analgesia and promoting wound repair, the Qudu-lotion have more curative effect.

Objective To investigate the effects of electro-acupuncture (EA) combined with repetitive transcranial magnetic stimulation (rTMS) on protein kinase A-cyclic adenosine monophosphate response element binding protein (PKA-CREB) signal transduction system after focal cerebral ischemia in adult rats and to explore the mechanism of EA combined with rTMS in treating ischemic brain injury.Methods The animal model of transient focal ischemia was made by artificial middle cerebral artery occlusion. Seventy-five Wistar rats were randomly divided into normal group, model group, EA group, rTMS group and EA+rTMS group. The expressions of PKA and pCREB in hippocampus were detected and the neurologic impairment rating was observed at the 7th, 14th and 28th days, respectively, after infarction.Results The average gray densities of PKA and pCREB expressions in hippocampus after focal cerebral ischemia in model group were higher at the 7th d, lower at the 28th d than that in normal group (P＜0.05);higher in EA group, rTMS group, EA+rTMS group than that in model group at all time points (P＜0.05), higher in EA+rTMS group than that in EA group and rTMS group at 7th and 14th d(P＜0.05), and there was no difference between EA group and rTMS group(P＞0.05).The improvement of neural motor function was obvious in EA group, rTMS group and EA+rTMS group compared with model group (P＜0.01), especially in EA+rTMS group.Conclusions EA combined with rTMS can promote the functional recovery after cerebral ischemia,enhance the expression of PKA-CREB signal transduction system in hippocampus after focal cerebral ischemia, which might be one of the important mechanisms of EA combined with rTMS in treating ischemia brain injury.

Objective　To investigate whether the p21ras/ERK (MAPK) cascade is involved in regulating the production of TNF-β and IL-2 in Jurkat cells. Methods　Cells were transiently transfected with a dominant negation construct of p21ras (ras15A or ras17N), raf (raf 1-130) or ERK1 (erk1K71R), or transforming p21ras (H-ras61L) or constitutively active raf (raf22W) using lipofectamine. Control cells received a plasmid carrying β-galactosidease. Transfectant cells were stimulated with PMA/PHA and released TNF-β and IL-2 quantitated by ELISA. Results　In control cells, the production of TNF-β and IL-2 was approx. 2 fold by PMA/PHA stimulation (P<0.001), while transfection with a dominant negative mutant construct abrogated the effects of PMA/PHA. Transfection with a constitutively active mutant had no effect on basal production of TNF-β or IL-2, but all of the constitutively active mutants significantly enhanced the response of the cells to PMA/PHA (P<0.05). MEK1 inhibitor PD98059 inhibited the effects of PMA/PHA and active construct genes on ERK activities and cytokine production. Conclusion　These data demonstrate that the p21ras-ERK cascade plays an important role in regulating the production of both TNF-β and IL-2 in Jurkat cells stimulated with PMA/PHA.

Objective To research the methods of treating gynecological inflammatory diseases with TCM. Methods By analyzing gynecological etiology and pathogenesis of inflammatory diseases, we explored the functions of pathogenic dampness, heat, blood stasis, and deficiency of genuine Qi on the onset, development and changes of gynecological inflammatory diseases. Results Gynecological inflammatory diseases is mainly caused by external invasion of pathogenic dampness, heat and insect poison and internal disorder of viscera, leading to the generation of internal dampness, heat, blood stasis and turbid fluid. Besides, the deficiency of genuine Qi is also a major reason for this disease. The therapeutic methods should be eliminating dampness, clearing heat, removing blood stasis, and strengthening genuine Qi. Conclusion TCM is effective in treating gynecological inflammatory diseases.

Objective The purpose of this study was to investigate the effect of psychological intervention and flurbiprofen axetil on the recovery period of laparoscopic surgery for sevoflurane anesthesia.Methods 100 cases of laparoscopic surgery in the First People's Hospital of Jiande City from January 2015 to December 2016 were selected.They were randomly divided into the control group and the experimental group, each with 50 cases.The induction methods of the control group and the experimental group were the same as those during the operation, and propofol and sevoflurane and remifentanil were used to maintain anesthesia.The experimental group patients before intravenous injection of 2 mg/kg flurbiprofen, psychological intervention nursing, pay attention to the psychological status of patients, strengthen the communication with patients, patients with heart to eliminate negative emotions, relieve the psychological pressure of patients.Results The incidence of restlessness before extubation was 32%in the control group,and the rate of restlessness immediately after extubation was 22%.The occurrence rate of restlessness was 56% at 5 minutes after extubation.In the experimental group, the incidence of agitation at different times was significantly lower than that in the control group, with statistical difference(P<0.05).Compared with the control group, the hemodynamics of the experimental group was more stable, and the incidence of nausea and other adverse reactions was lower, with statistical difference(P<0.05).Conclusion Laparoscopic surgery under sevoflurane anesthesia can reduce the incidence of restlessness in recovery period of a certain degree of psychological intervention combined with flurbiprofen,stable hemodynamics,reduce the incidence of adverse reactions.

Guided by the theory of constructivism,we utilize the internet resource to design the teaching of medical ethics under the condition of information,change the traditional teaching method and utilize the new teaching ideas.When teaching knowledge,we also advocate students'ability,attitude and quality.

OBJECTIVE:To provide reference for breaking the“dual-track”system of the pharmacist and perfecting pharmacist system in China. METHODS:By reviewing laws and regulations relating to the pharmacist in recent 21 years,the implementation and problems of pharmacist system was investigated. RESULTS & CONCLUSIONS:The imperfection and dissonance of pharma-cist management regulations and laws system result in long-term“dual-track”system of pharmacist authentication. There still are other problems such as low rate of pharmacist registration and unbalanced regional distribution;unbalance between national li-censed pharmacist examination and examination condition of health systems pharmacist;divergence and mismatching between phar-macy education pharmacist training;social value of pharmacists cannot be reflected adequately. It is suggested to formulate Regula-tions on the Licensed Pharmacists as soon as possible,establish uniform and standard qualification and title appraisal system for na-tional pharmaceutical professional and technical personnel,promote licensed pharmacist classification registration and examination system reform,strengthen pharmacy education and licensed pharmacist training system reform,guide licensed pharmacist allocation rationally and orderly by various measures and enhance the propaganda of licensed pharmacist and their work,so as to promote the mergence of national licensed pharmacists qualification system and health professional technical pharmacist competence system.

Systemized ethics is the evitable choice of the history development. The rise of systemized ethics is the important change of emphasis on morality construction. The implement of systemized ethics is not only beneficial to organically integrate universality and advance, but also realize the moral-living theory. The exertion of function of systemized ethics depends on the degree of systemized ethics.