1.Organ allografts combined with hematopoietic stem cell transplantation and immunotolerance
Chinese Journal of Hepatobiliary Surgery 2010;16(12):963-965
Our understanding of the immunological mechanisms of rejection has greatly improved over the past 10 years. The allografts have maintained long survival without immunosuppressive treatment in the several organ-transplant recipients combined with hematopoietic stem cell transplantation. The authors attributed the successful outcome to the cotransplantation of donor stem cells. The review will analyze firstly conditions of the recipients, and then review the theoretical basis and history regarding donor-specific tolerance of allograft, and it will also clarify that there is no direct link between the donor-specific transplantation tolerance and hematopoietic stem cell transplantation.
2.Analysis on division of specialists and general practitioners in China:From the perspective of Labor Division theory
Chinese Journal of Health Policy 2015;(2):8-12
The division of specialists and general practitioners is the important of the establishment of a GP system. According to the theory of labor division, the study analyzes the evolution mechanism on the division of spe-cialists and general practitioners and discusses the main factors influencing the division in China. It is found that transaction costs of general practices are not much lower than that of specialist medical care, which encourages outpa-tients to visit specialists in hospitals. Additionally, transaction costs of referral are much higher than profits. There-fore, while there is no division of specialists and general practitioners, it has been difficult to address competition between them and the quality and the efficiency of general practice care in community health centers. Moreover, the income gap does not attract medical students to work in general practices, which has worsened the quality and effi-ciency of general practices. Finally, the transaction costs of general practices have continually increased. This vicious cycle has hindered the division of specialists and general practitioners in China.
3.Follow-up after treatment of CIN2~3
Basic & Clinical Medicine 2006;0(12):-
Treatment of CIN2~3 includes cervical ablation,cervical local excision and hysterectomy,while cervical local excision is the standard procedure for CIN2~3.The recurrence rate was 0.7%~21% after surgery treatment of CIN2~3;The high risk factors of recurring are positive margin,serious lesion and age over 40.The follow-up items for postoperation involve cytology,colposcopy,ECC and HPV testing;If there were 2 LSIL or 1 HSIL for cytology,the patients should be given colposcopy examination and ECC.Retreatment must be clinically individual one.It is the same significance for follow-up and treatment of CIN2~3.
4.Determination of Naringin in Davallia mariesii by Accelerated Solvent Extration-HPLC
China Pharmacy 2016;27(27):3875-3876,3877
OBJECTIVE:To develop a new way for the extration and determination of naringin in Davallia mariesii. METH-ODS:Naringin in D. mariesii was extracted by ASE350 accelerated solvent extraction system;HPLC was performed for the con-tent determination of naringin in D. mariesii,the column was Kinetex XB-C18 with mobile phase of methanol-5%acetic acid(25∶75, V/V)at a flow rate of 0.8 ml/min,detection wavelength was 283 nm,column temperature was 40℃,and injection volume was 10 μl. RESULTS:The linear range of naringin was 0.073 0-0.730 0 μg(r=0.999 9);the limit of quantitation was 0.73 μg,the limit of detection was 0.022 μ g;RSDs of precision, stability and reproducibility tests were lower than 2% ;recovery was 98.92%-100.85%(RSD=0.72%,n=6). CONCLUSIONS:The method is simple,accurate and specific,and can be used for the content determination of naringin in D. Mariesii.
5.Effect of seretide combined with singulair on lung function improvement and prognosis of moderate to severe AECOPD
Xiaochun HUANG ; Qier DAI ; Lingfeng DAI
Chinese Journal of Biochemical Pharmaceutics 2017;37(2):252-254
Objective To study the effect improvation of lung function and prognosis of singulair and seretide for moderate to severe AECOPD . Methods 78 patients associated with moderate to severe AECOPD from January 2014 to June 2016 in our hospital were researched.They were divided randomly into observation and control groups according to the order of admission, 39 cases in each group.In the control group, they were treated by conventional anti-infective, expectorant, oxygen, suction seretide.In the observation group, using singulair on the basis of the control group.Clinical data, clinical efficacy, clinical symptoms begin to improve time, hospitalization time, blood gas analysis (PaO2, PaCO2) after five days and pulmonary function after treatment forced expiratory volume in one second ( FEV1 ) , call gas peak velocity ( PEF) , forced expiratory volume in one second than the forced vital capacity (FEV1/FVC), maximum mid-expiratory flow rate (MMEF) were compared with each other.Results Their clinical data of patients and other general information and basic condition had no significant difference between each other.The effective rate of observation group and control group were 97.44%and 87.18%, clinical effecicy of observation group was better than the control group, the difference was statistically significant (u=2.2805, P<0.05).Postoperative hospital stay, time of symptoms began to improve of the observation group were shorter than the control group, the difference was statistically significant (P<0.05), PaO2 of 5-day post-treatment of observation group was higher, and PaCO2 was lower, the difference was statistically significant (P<0.05).Lung function of two groups of patients had no significant difference before treatment, after treatment, pulmonary function in the observation group (including FEV1, PEF, FEV1/FVC, MMEF) was better than the control group, the difference was statistically significant (P<0.05).Conclusion Seretide combined with singulair to treat severe AECOPD has a good effect, it can significantly improve the therapeutic effect and improve the patient's lung function.
6.Establishment of teaching evaluation index system for clinical practice of nursing specialty in uni-versities
Lihuo LI ; Shuangli HUANG ; Yingbo DAI
Chinese Journal of Practical Nursing 2009;25(16):6-7
Objective To establish a set of teaching evaluation index system for clinical practice which can embody education cocept of modern nursing,accord with reform direction of nursing education, and is scientific,reasonable and easy to operate. Methods Teaching evaluation index system for clinical practice was preliminarily established using literature data, theoretical analysis, expert evaluation,collective argument by evaluator and who were evaluated. Results 32 first-order indices,169 second-order indices and value assignment of weight were confirmed after establishment of teaching evaluation index system, which was satisfying to the evaluator, who were evaluated and nursing students. Conclusions The evalu-ation index satisfy the requirement of teaching quality evaluation for practice,the degree of approval, coordi-nation and reliability is relatively high, so it can be used for teaching quality evaluation for nursing practice.
7.Mitochondrial DNA haplotype and mutations related with aminoglycoside-induced deafness.
Qi LI ; Pu DAI ; De-Liang HUANG
Chinese Journal of Otorhinolaryngology Head and Neck Surgery 2008;43(6):474-476
DNA, Mitochondrial
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genetics
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Deafness
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chemically induced
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genetics
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Haplotypes
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Humans
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Mutation
8.Clinical features and treatments of pediatric acute and severe diseases initially presented with abdominal pain
Mei LIU ; Mei DAI ; Yongkun HUANG
Chinese Pediatric Emergency Medicine 2011;18(6):494-496
Abdominal pain is one of the common symptoms in children with diseases.Acute abdominal pain accounts for some parts of all primary presenting diseases in the emergency department.The clinical features and treatments of fulminant myocarditis,Henoch-Schonlein purpura and diabetic ketoacidosis initially presented with abdominal pain in children were reviewed in the article.
9.Effects of Angongniuhuang Pill on the Synthesis of ApolipoproteinE in Brains of Rats after Traumatic Brain Injury
Zhen XU ; Lifa HUANG ; Yaguang DAI
Journal of Zhejiang Chinese Medical University 2006;0(04):-
[Objective] To explore the effects of angongniuhuang pill on the synthesis of apolipoprotein E in brains of rats after traumatic brain injury.[Methods] Using reverse transcription-polymerase chain reaction(RT-PCR) and enzyme linked immunosorbent assay(ELISA),the apolipoprotein E’s mRNA of contused brain tissue and the concentration of apolipoprotein E in CSF at different phases after traumatic brain injury were detected of rats fed with angongniuhuang pills.[Results]High expression of apolipoprotein E mRNA and high concentration of apolipoprotein E were found in rats fed with angongniuhuang pills compared with controls.[Conclusion]Angongniuhuang pills may increase the expression of apolipoprotein E mRNA in brains after traumatic brain injury,thus promote the synthesizing and secreting of apolipoprotein E.
10.Decreasing bleeding effect of transamin on the induced abortion
Zirong HUANG ; Aihua FANG ; Zhongying DAI
Chinese Journal of Obstetrics and Gynecology 2001;0(07):-
0 05) between the two groups in the patient′s age, pregnancy age, parities, the cavity volume of uterine, hemoglobin and blood platelet count The bleeding volume was (51 6?17 2) ml for the treated group and (63 3?17 1) ml for the control group ( P 2 months It could decrease the volume of bleeding during the operation and benefit for patients recovering