1.Effect of Moxa Smoke on Amino Acids Neurotransmitters in Brain of Senescence Accelerated Mice
Yingxue CUI ; Huanfang XU ; Ping LIU ; Juntian LIU ; Baixiao ZHAO
Chinese Journal of Information on Traditional Chinese Medicine 2013;(10):29-31
Objective To observe the effect of moxa smoke (Artemisia vulgaris) on amino acids neurotransmitters in the brain of senescence accelerated mouse (SAMP8) and explore the anti-aging effect of moxa smoke. Methods Totally 70 SAMP8 were randomized into one model control group and 6 intervention groups, and 10 SAMR1 mice were used as normal control group. There were low, medium and high concentrations of moxa smoke during intervention. Moxa smoke intervention was performed 15 min/30 min each day for 28 days. High performance liquid chromatographic method was used to determine the levels of Glu, Asp and GABA in the brain of the mice. Results Compared with the normal control group, the levels of Asp and Glu in the model control group were significantly higher, while the GABA was significantly lower. The levels of Asp and Glu in 6 intervention groups were significantly lower than those in the model control group, while GABA was significantly higher than or the same as the model control group. There was no significant difference among different intervention groups in the levels of Asp and Glu, while the 30 min-medium concentration moxa smoke had the most significant effect in increasing the level of GABA. Conclusion Moxa smoke intervention could decrease the high level of Asp and Glu, and increase the low level of GABA in SAMP8. It could achieve the effect of anti-aging through adjusting the abnormal metabolism of amino acids in the brain.
2.Influence of EGFR in B[a]P-induced SIRT1 activation in human bronchial epithelial cells
Min ZHANG ; Yunqin CUI ; Huanfang SONG ; Jianyi LYU ; Xiaohong XU ; Jimin GAO
Journal of Jilin University(Medicine Edition) 2016;42(4):731-736
Objective:To investigate the role of epidermal growth factor receptor (EGFR)in the regulation of B[a]P-induced silent information regulator 1 (SIRT1 ) activation in the human bronchial epithelial cells (BEAS-2B),and to clarify the relationship between EGFR/SIRT1 signal transduction pathway and the occurrence and development lung cancer.Methods:The prediction analysis of transcriptional factor binding sites of SIRT1 was performed by MOTIF SearchTM software.The primary cultivated BEAS-2B cells were divided into control group (without B[a]P exposure)and B[a]P groups (the cells were exposed to B[a]P for 6,12,24,48 h).RT-PCR and Western blotting method were carried out to detect the EGFR mRNA and protein expression levels.The BEAS-2B cells transfected with SIRT1 promotor luciferase reporter gene plasmid were treated with human epidermal growth factor (hEGF)and Genistein (tyrosine protein kinase inhibitor)for 24 h,the morphology of BEAS-2B cells was observed by inverted microscope, and luciferase reporter assay was used to test the SIRT1 transcriptional activity.The human lung tissue biopsies were acquired and the immunohistochemical analysis was used to determine the EGFR protein expression level.Results:The transcriptional factor binding sites of SIRT1 contained EGF, 2Fe-2S and vWF.Compared with control group,the expression levels of EGFR mRNA in B [a]P groups were increased in a time-dependent manner,and reached the peak at 12 h (P < 0.05);the EGFR protein expression levels were also increased (P <0.05).The SIRT1 luciferase activity in hEGF group was increased compared with control group (P <0.05);when hEGF and B[a]P worked together,the SIRT1 luciferase activity was increased even further (P <0.001). The cells showed arrangement and morphologic changes gradually when the B[a]P concentration was above 30 μmol · L-1. Genistein (30 μmol · L-1 )inhibited the increase of SIRT1 luciferase activity induced by B [a]P ,and there was significant difference compared with control group (P <0.05).The immunohistochemistry results showed that EGFR expression level in lung cancer tissue was higher than that in normal lung tissue (P < 0.001).Conclusion:EGFR can regulate the B [a]P-induced SIRT1 expression in BEAS-2B cells,and to cause lung chronic inflammation;EGFR/SIRT1 signal transduction pathway may play a role in the occurrence and development of lung cancer.
3.Study on the Improvement Effect and Mechanism of Ilicifoliosids Alkaloid A on Non-alcoholic Fatty Liver Di- sease in Rats
Wanpeng XU ; Yingqin LIANG ; Xiugui WEI ; Hongyuan WANG ; Hua ZHANG ; Huanfang ZHOU ; Xing LIN ; Jun LIN
China Pharmacy 2020;31(16):1955-1960
OBJECTIVE:To s tudy improvement effect and mech anism of ilicifoliosids alkaloid A (HBOA)on non-alcoholic fatty liver disease in rats. METHODS :SD rats were randomly divided into blank control group ,model group ,Silybin capsule group(positive control ,26.25 mg/kg),HBOA high-dose ,medium-dose and low-dose groups (100,50,25 mg/kg),with 10 rats in each group. Except that blank control group fed normal feed ,the other groups were continuously fed with high-fat diet for 8 weeks to induce non-alcoholic fatty liver disease model. Form the 9th week ,blank control group and model group were given constant volume of 0.6% CMC-Na solution ,and administration groups were given corresponding drugs by intragastric admini- stration,once a day ,for consecutive 4 weeks. The general information of rats were observed and the body weight increase ,organ (liver,kidney and spleen )indexes were calculated ;the contents of AST ,ALT,TC,TG and NEFA in liver tissue were detected , and SOD,GSH-Px activities and MDA content in the serum were also determined. The protein expression of PPARα in liver tissue was detected by immunohistochemistry. RESULTS :Compared with blank control group ,the body mass increase and liver index of rats in model group were increased significantly (P<0.01);fat deposition could be observed in the liver ;the activities of SOD and GSH-Px in serum were reduced significantly ,and the contents of MDA ,the contents of AST ,ALT,TC,TG and NEFA in liver tissue were significantly increased ,and the protein expression of PPARα was decreased significantly(P<0.01). Compared with model group ,the body mass increase and liver index of the rats were decreased significantly in administration groups (P<0.05 or P<0.01),liver fat deposition was improved ,the activity of SOD and GSH-Px in serum (except for HBOA low-dose group )were increased significantly while MDA content ,the contents of AST ,ALT,TC(except for HBOA low-dose group ),TG(except for HBOA low-dose group ) and NEFA in liver tissue were decreased significantl y,while protein expression of PPAR α 15177460685@163.com was increased significantly (P<0.05 or P<0.01). Some of the above indexes of HBOA high-dose group were 电话:0771-5302433。E-mail:junlin898@126.com significantly better than HBOA medium- and low-dose group(P<0.05). CONCLUSIONS :HBOA has a certain improvement effect on non-alcoholic fatty liver disease in rats caused by high-fat diet ,and its mechanism may be related to improving lipid metabolism disorders ,anti-oxidative stress and up-regulating the expression of PPARα.
4.Acupuncture with regulating menstruation to promote pregnancy for diminished ovarian reverse: a prospective case series study.
Xiaotong LI ; Huanfang XU ; Yigong FANG ; Jie SHANG ; Huisheng YANG ; Xuelin ZHOU ; Minglong ZHANG
Chinese Acupuncture & Moxibustion 2017;37(10):1061-1065
OBJECTIVETo investigate the effect and safety of acupuncture with regulating menstruation to promote pregnancy for diminished ovarian reverse (DOR).
METHODSAccording to prospective case series, 46 patients were observed and finally 40 cases were included. The acupoints were ① Baihui (GV 20), Shenting (GV 24), Guanyuan (CV 4) and bilateral Benshen (GB 13), Huangshu (KI 16), Dahe (KI 12), Luanchao (Extra), Zusanli (ST 36), Sanyinjiao (SP 6), Taixi (KI 3), Taichong (LR 3) and ② bilateral Shenshu (BL 23) and Ciliao (BL 32). The points in the two groups were used alternately. Acupuncture was given for 3 courses, 12 times as a course and 3 times a week. Before and after treatment, and 3 months after treatment, follicle-stimulating hormone (FSH), follicle-stimulating hormone/luteinizing hormone (FSH/LH), estradiol (E), antral follicle count (AFC) and TCM symptom score were observed. The safety was evaluated.
RESULTSCompared with before treatment, the levels of FSH, FSH/LH decreased, and the levels of Eand AFC increased after treatment and at follow-up (all<0.05). And the TCM symptom scores were significantly lower than those before treatment (both<0.05). The rate of pregnancy after treatment was 15% (6/40). There was no infection and organ injury.
CONCLUSIONAcupuncture with regulating menstruation to promote pregnancy can safely improve the ovarian reserve of patients with DOR.
5.Effects of 4-hydroxy-2(3H)-benzoxazolone on Inflammatory and Apoptosis Signaling Pathways in Non-alcoholic Fatty Liver Disease Model Rats
Wanpeng XU ; Jun LIN ; Yingqin LIANG ; Huanfang ZHOU ; Hua ZHANG ; Shizhen HUANG ; Xuemei SUN ; Xiugui WEI ; Hongyuan WANG ; Lin LIU
China Pharmacy 2021;32(11):1298-1303
OBJECTIVE:To inv estigate the effects of 4-hydroxy-2(3H)-benzoxazolone on inflammatory and apoptosis signaling pathways in non-alcoholic fatty liver disease (NAFLD)model rats. METHODS :SD rats were divided into normal control group(10 rats)and modeling group (50 rats). Normal control group was given basic diet ,and modeling group were given high-fat diet to induce NAFLD model. After modeling ,the rats were divided into normal control group ,model group ,silibinin group (26.25 mg/kg),and 4-hydroxy-2(3H)-benzoxazolone high-dose ,medium-dose and low-dose groups (100,50,25 mg/kg),with 8 rats in each group. Normal control group and modeling group were given 0.6% CMC-Na intragastrically ,and other groups were given relevant medicine 10 mL/kg intragastrically ,once a day ,for consecutive 4 weeks. After last medication ,the serum levels of albumin(ALB),total protein (TP),globulin(GLB),ALB/GLB and free fatty acid (FFA)were detected ;TUNEL staining was used to observe the apoptosis of rat hepatocytes. Western blot assay was used to detect the protein expression and phosphorylation level of inflammatory signaling pathway related proteins [Toll-like receptor 4(TLR4),myeloid differentiation factor 88(MyD88), nuclear factor-κB p65(NF-κB p65),NF-κB inhibitor protein(IκBα)] in liver tissue as well as the expression of apoptosis signaling pathway related proteins [B cell lymphoma 2(Bcl-2),Bax,caspase-3]. RESULTS :Compared with model group ,serum levels of TP (except for low-dose group ),GLB and FFA ,the protein expression of TLR 4(except for low-dose group ),MyD88 (except for medium-dose group )and caspase- 3,the phosphorylation levels of NF-κB p65 and IκBα protein were decreased significantly(P<0.05 or P<0.01). The ratio of A LB/GLB in serum and the ratio of Bcl- 2/Bax in liver tissue were significantly increased(P<0.05 or P<0.01),and the phenomenon of hepatocyte apoptosis was improved. CONCLUSIONS :4-hydroxy-2 (3H)-benzoxazolone can ameliorate NAFLD in rats ,and the mechanism may be associated with inhibiting the expression TLR 4/ MyD88/NF-κB signaling pathway-related proteins and apoptosis-related proteins in liver tissues.
6.Comments on " " from .
Huisheng YANG ; Xiaotong LI ; Jie SHANG ; Huanfang XU ; Yaqian YIN ; Mingzhao HAO ; Yigong FANG
Chinese Acupuncture & Moxibustion 2018;38(12):1341-1346
In recent years, acupuncture has been used widely as an adjuvant treatment for the in vitro fertilization (IVF). " " published in on May 15, 2018, the research findings do not support the use of acupuncture to improve the rate of live births among the women undergoing IVF. In order to avoid the misunderstanding of the scholars at home and abroad for the clinical effects of acupuncture on IVF assistance, the authors put forward the doubts after the analysis from the following 3 aspects, including the acupuncture scheme, outcomes and result explanation. Additionally, the thinking and suggestions are proposed for the future development of the clinical trials of acupuncture IVF assistance in terms of selecting the proper participants, being abided by the standards of the evidence-based medicine, designing multi-acupuncture schemes and setting up the rational control, as well as conducting the overall analysis of the trial data.
Acupuncture
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American Medical Association
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Embryo Transfer
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Female
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Fertilization in Vitro
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Humans
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Live Birth
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Pregnancy
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Pregnancy Rate
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United States
7.Dosage of 4-Vinylcyclohexene Diepoxide in Induction of Premature Ovarian Insufficiency in Rats
Weixin LI ; Pengfei DU ; Yaoyao ZHU ; Chenchen SU ; Huanfang XU ; Li YANG ; Xiaojing SONG ; Yigong FANG
Chinese Journal of Experimental Traditional Medical Formulae 2024;30(18):72-79
ObjectiveTo compare the effects of different doses and withdrawal time of 4-vinylcyclohexene diepoxide (VCD) on the reproductive endocrine levels of female rats, and to explore the effective, stable, and safe dosage of VCD for constructing a premature ovarian insufficiency (POI) rat model. MethodSD rats with regular estrous cycles were randomly divided into three groups: blank group, low-dose VCD group (80 mg·kg-1·d-1), and high-dose VCD group (160 mg·kg-1·d-1), with 24 rats in each group. After drug intervention, samples were collected on the 15th day (D15) and the 45th day (D45) after intervention. The general condition, rate of estrous cycle disturbance, serum hormone levels, ovarian histomorphology, follicle count, pregnancy outcome, and the protein and mRNA expression of transforming growth factor (TGF)-β and Smad2/3 were assessed. ResultCompared with the blank group, the low-dose VCD group showed no significant differences in the rate of estrous cycle disturbance or serum follicle-stimulating hormone (FSH), luteinizing hormone (LH), and estradiol (E2) levels. Ovarian tissue was damaged. Specifically, the number of primordial and primary follicles decreased on D15 (P<0.01), and the number of secondary follicles (P<0.01) and antral follicles (P<0.05) further decreased on D45. The litter number decreased on D15 (P<0.05), but there was no significant difference on D45. Furthermore, TGF-β protein levels increased on D15 (P<0.05) and D45 (P<0.01). The Smad2/3 levels increased on D45 (P<0.01), and TGF-β and Smad2/3 mRNA levels increased on D45 (P<0.05). Compared with the results in the blank group, the disturbance rate of the estrous cycle increased on D45 in the high-dose VCD group (P<0.01). The serum of FSH and LH increased (P<0.01), while E2 decreased (P<0.05). Ovarian tissue was damaged, and the downward trend of follicles at all levels was similar to that in the low-dose VCD group. The litter number significantly decreased on D15 and D45. TGF-β and Smad2/3 protein levels increased (P<0.05,P<0.01), and TGF-β mRNA increased on D45 (P<0.05). ConclusionHigh-dose VCD is an ideal method for constructing a POI rat model, being effective, stable, and safe.