Objective To investigate the epidemiological characterization , effect of prevention and control measures during an outbreak in a military unit caused by Mycoplasma pneumoniae.Methods All the cases were investigated to learn about their basic characteristics , clinical symptoms and aggregation activities using epidemiological cross-sectional study . Results From May 16 to June 11,2013, forty-nine confirmed cases and thirty-five suspected cases who were all males were found at an attack rate of 7.8%.The epidemic peak period was from May 27 to Jun 2(seven days), and the number of total cases was 66(75.9%).During the outbreak, the attack rate of the 10th cadet team, other cadet teams and a non-student population was 47.5%, 4.5% and 1.2% respectively.The difference was significant (P<0.01).The age of forty-nine confirmed cases ranged from 17 to 26 years old , the attack rate did not differ significantly between different age groups, and no aggregation was found in the place of birth and soldier source .The most frequent clinical symptoms were fever and cough for the confirmed cases , 62.5% of whom showed unilateral or bilateral lung inflammation change , while few positive lung symptoms were present .All the confirmed cases were hospitalized and no severe or critically ill cases were present.The outbreak was controlled effectively after implementation of case surveillance , contact precaution , termination of aggregation activities , dispersed habitation and prescription of azithromycin for disease prevention .Conclusion The confined and crowed environment for learning and training can increase the risk of M.pneumoniae outbreak , making it nec-essary to enhance disease prevention awareness , improve case management , live dispersed , reduce close contact and imple-ment personal hygienic measures .

Objective To explore the analgesic mechanisms of sufentanil. Methods 17 (8 male, 9 female) healthy right-handed volunteersreceived functional magnetic resonance imaging (fMRI) scan before and after sufentanil 0.2 ng/ml administration respectively. Thalamuswas as the region of interest (ROI). All the processing works were carried out using the Statistical Parametric Mapping. Results Comparedwith the basic state, significantly weaker functional connectivity was mainly found in the region of bilateral rectal gyrus and the left inferiaorbital gyrus of the frontal lobe, while significantly greater functional connectivity was found in bilateral cerebellum, the right cingulategyrus and the left middle temporal gyrus (P<0.001, cluster>13 mm3). Conclusion Thalamus plays a key role in the central analgesia ofsufentanil, which associated with the functional connectivity of bilateral rectal gyrus and the left inferia orbital gyrus of the frontal lobe, bilateralcerebellum, the right cingulate gyrus and the left middle temporal gyrus.

Objective To explore the analgesic mechanisms of sufentanil. Methods 17 (8 male, 9 female) healthy right-handed volunteersreceived functional magnetic resonance imaging (fMRI) scan before and after sufentanil 0.2 ng/ml administration respectively. Thalamuswas as the region of interest (ROI). All the processing works were carried out using the Statistical Parametric Mapping. Results Comparedwith the basic state, significantly weaker functional connectivity was mainly found in the region of bilateral rectal gyrus and the left inferiaorbital gyrus of the frontal lobe, while significantly greater functional connectivity was found in bilateral cerebellum, the right cingulategyrus and the left middle temporal gyrus (P<0.001, cluster>13 mm3). Conclusion Thalamus plays a key role in the central analgesia ofsufentanil, which associated with the functional connectivity of bilateral rectal gyrus and the left inferia orbital gyrus of the frontal lobe, bilateralcerebellum, the right cingulate gyrus and the left middle temporal gyrus.

Objective To investigate the expressions of phosphorylated collapsin response mediator protein 2(pCRMP2)and class Ⅲ β-tubulin protein(Ⅲ β tubulin)in elderly patients with stage Ⅲ/Ⅳ non-small cell lung cancer (NSCLC),and to evaluate the value of the expressions of pCRMP2 and Ⅲβ tubulin in predicting the chemotherapeutic sensitivity of taxol.Methods A total of 127 elderly patients with stage Ⅲ/Ⅳ NSCLC were recruited,and expression levels of pCRMP2 and Ⅲ β tubulin were detected by immunohistochemistry and Western blot.Based on the levels of pCRMP2 and Ⅲ β tubulin expression,all patients were divided into four groups,including group A(low expressions of both pCRMP2 and lβ tubulin),group B(high expressions of both pCRMP2 and Ⅲβ tubulin),group C(high expression of only pCRMP2)and group D(high expression of only Ⅲ β tubulin).All patients were assigned to receive two cycles of TP chemotherapy regimens and then evaluate the effect of chemotherapy.If chemotherapy was invalid,a second-line chemotherapy regimen was administered.Relationships of response rate,overall survival(OS)and time on tumor progression(TTP) with expression levels of pCRMP2 and Ⅲ β tubulin protein were assessed.Results Among 127 elderly patients,the high expression rate of pCRMP2 and Ⅲ β tubulin protein were 51.2％ (65 cases) and 47.2％ (60 cases),respectively.The Western blot result displayed that the high expressions of pCRMP2 and Ⅲ β tubulin protein were significantly associated with pathologic stage,and with poorly differentiated adenocarcinoma(all P＜0.05).The pCRMP2 expression was also related to the clinical stage.Meanwhile,the overall survival rate(OS)was significantly lower in patients with high expression of pCRMP2 and Ⅲ β tubulin protein than in patients with low expression of the two proteins(P＜ 0.05).Expression of pCRMP2 and Ⅲβ tubulin protein was related with pathological grade and tissue classification.The remission rate (RR)was significantly higher in group A(65.3 ％)than in other three groups(RR in group C,group D and group B was 48.1％,44.0％,30.8％;all P＜0.05,respectively).There were significant differences in RR between group C,group D and group B(48.1 ％,44.0％,30.8％ respectively,P＜0.05).The OS of four groups were(510.5 ±20.9)d,(215.7±31.5)d,(326.9 ± 25.7)d,(298.7±21.2)d respectively.The OS was significantly longer in group A than in other groups(P ＜ 0.05).Conclusions The expression of pCRMP2 and Ⅲ β tubulin protein in NSCLC can be used to judge prognosis of lung cancer and to predict chemotherapy drug resistance.