Administration, Inhalation ; Adrenal Cortex Hormones ; administration & dosage ; adverse effects ; therapeutic use ; Asthma ; drug therapy ; Child ; Dose-Response Relationship, Drug ; Humans ; Pediatrics ; trends

Animals ; Antigens, Dermatophagoides ; genetics ; immunology ; Arthropod Proteins ; Asthma ; etiology ; therapy ; Humans ; Mice ; Vaccination ; Vaccines, DNA ; immunology

Humans ; Pleural Effusion ; etiology ; immunology ; T-Lymphocytes, Regulatory ; physiology

Objective: To control the quality of Jinqiantonglin Oral Liquid and establish the determination of chlorogenic acid in the liquid. Methods: HPLC was used to determine the content of chlorogenic acid on ODS-2 column with a mobile phase of methanol-acetonitrile-water(10.5∶2.5∶87). pH was adjusted to 3.0 by acetic acid. UV detection wavelength was set at 325nm. Results: The linearity of chlorogenic acid standard was well and the average recovery was 100.07%, RSD was 1.47%(n=6). Conclusion: This method is convenient, quick, accurate and available for the quality control of Jinqiantonglin Oral Liquid

Objective To evaluate the efficacy of retroperitoneal laparoscopic renal cystectomy.Methods A total of 80 patients with renal cyst were enrolled in this study.Under general anesthesia,the retroperitoneal cavity was established by using water-filled balloon.The renal cyst were exposed by identifying the psoas major muscle.The cysts were unroofed at 5 mm from the edge of the renal parenchyma with electrical claw.Results The operation was completed in all the cases without severe complications.The operation time was 20-100 min(mean 39 min),blood loss was 5-65 ml(mean 20 ml),and hospital stay was 4-11 d(mean 5.6 d).Postoperative pathological examination showed that all the cysts were simple renal cysts.The 80 patients were followed up for 2-24 months(mean 11 months),3 of them were diagnosed as having residual cyst at 2 months after the operation.Conclusion Retroperitoneal laparoscopic renal cystectomy is safe and effective and is superior in mild surgical trauma,quick recovery,and short hospitalization.

Adrenal Cortex Hormones ; therapeutic use ; Adrenergic beta-Agonists ; therapeutic use ; Anti-Asthmatic Agents ; therapeutic use ; Asthma ; drug therapy ; Humans ; Medication Adherence

Antibodies ; immunology ; therapeutic use ; Asthma ; drug therapy ; Humans ; Interleukin-5 ; immunology

[Objective]To study NGF,BDNF gene modified bone marrowe stem cells transplantation with vein on spinal cord injury.[Method]Spinal cord injured rats were divided in to 3 groups.Group C consisted of the rats treated with the transplantation of modified NGF,BDNF genes BMSCs;group B of the rats with transplantation of BMSCs without genetic modification;group A of the rats without treatment and group D was the normal contral.At 2,6,10 weeks the rats were examined with GBS score and neurological function were tested.[Result]The vein with tintracord transplantation of modified BMSCs group was improved by the GBS score and neurological outcome of injured spinal cord was achieved well.[Conclusion]The vein with transplantation of modified NGF,BDNF genes BMSCs can recover of spinal cord injury.

BACKGROUND: Heat-activated nickel-titanium (HANT) wire is a new type of arch wire in orthodontic treatment, characterizing by well memory alloy ability and uneasily deformation. However, environmental temperature may affect its performance.OBJECTIVE: To compare the effects of HANT and common nickel-titanium wire in clinical application.METHODS: A total of 80 patients who have accepted and finished orthodontic treatment in the First Affiliated Hospital of Sun,Yat-sen University from July 2006 to November 2008 were randomly divided into two groups. The common group was treated with common arch wire in aligning and leveling stages, while the HANT group was treated using HANT in aligning and leveling stages. Tooth extracted cases were matched to non-tooth extracted cases. Indicators, such as time span for alignment, total treating time, times for wire breaking, times for bracket shedding, and times for return visits, were measured.RESULTS AND CONCLUSION: There was significant difference in treating time and times for wire breaking between the two groups (P < 0.05), but there was no significant difference in the times for bracket shedding. Anaphylactic response, toxic reaction,and other adverse reactions were not observed in the HANT group. Therefore, HANT wire can simplify orthodontic operation,shorten operation time beside the chair, save the total treating time, and reduce chances of shedding brackets and breaking wires.

BACKGROUND: Researches indicate that interleukin-6 (IL-6) in blood has changed during the process of the edema and infection of spinal cord.It is found that IL-6 participates in the course of spinal cord lesion according to the further researches. It is also presumed that the IL-6 is correlated with the edema of spinal cord accompanied nervous system diseases.OBJECTIVE: To observe the effect of the receptor antagonist of the IL-6 on the traumatic edema of spinal cord, and explore the effect of IL-6 in the process of the edema of spinal cord.DESIGN: Randomized control experiment with animals as subjects.SETTING: Department of Orthopedic, Hospital Affiliated to Guangdong Medical College.MATERIALS: The experiment was completed from January to December 2004 in the Animal Experimental Center of Guangdong Medical College.Forty male Wistar rats were selected and randomly divided into the normal group, the wound group, the saline control group and the receptor antagonist of the IL-6 treated group with 10 rats in each group.METHODS: ① The receptor antagonist of the IL-6 treated group: Blast injury of the thoracic spinal cord was made by the sap pressure, and the injection with the receptor antagonist of the IL-6 was given at the injured region with microinjector. ② The wound group: There was not the procedure of administration and placement of the tube. ③ The saline control group: The IL-6 was replaced with the sterile-physiological saline solution (SPSS), and the other procedures were the same as those of the receptor antagonist of the IL-6 treated group. ④ The normal group: There was not any treatment. All rats in each group were detected with magnetic resonance. The water content in the spinal cord was determined with the dry and humid weight method, and the status of the edema of spinal cord was observed with the electron microscope and the pathological image analysis after dyeing with hematine-eosine.MAIN OUTCOME MEASURES: ① The results of the examination of the rats in each group with the magnetic resonance. ② The water content in the spinal cord of rats in each group. ③ The status of the edema of spinal cord was observed with the electron microscope and the pathological image analysis.RESULTS: All the 40 rats entered the result analysis. ① The edema of the T2 w eighting image in the receptor antagonist of the IL-6 treated group was relieved as compared with that in the wound group and the saline control group. ② The water content of the injured region in the receptor antagonist of the IL-6 treated group, the wound group and the saline control group was higher than that in the normal group [ (81.68±1.39)%, (79.72±1.49)%, (82.59±1.12)%, (77.19±0.64)% ,P ＜ 0.01].The water content of the injured region in the wound group was higher than that in the normal group(P ＜ 0.01), and the receptor antagonist of the IL-6 treated group was lower than that of the wound group (P ＜ 0.01). ③The cell body of the gray substance was contracted, the inter-space around the cell was enlarged and the nerve cells were lost in the wound group;The saline control group was not significantly different as compared with the wound group; The cellular edema, degeneration and the necrosis in the receptor antagonist of the IL-6 treated group was milder than that in the wound group.CONCLUSION: The receptor antagonist of the IL-6 has the therapeutical effect on the traumatic edema of spinal cord. The endogenous IL-6 participates in the development of the traumatic edema of spinal cord.