The periodontal regeneration is based on the proliferation and mult-differentiation of periodontal ligament cells.Thus,to find a method of enhancing the differentiation of periodontal ligament cells has been a hot point in studying periodontal disease.Many bioactive factors have important effects on the periodontal regeneration. Growth factors as bioactive factors have promotion effects on migration,growth,proliferation,differentiation,and protein synthesis.

Objective To observe and explore the clinical curative effect and mechanism of four grinding soup oral liquid in treating pediatric indigestion.Methods 90 children with indigestion were randomly divided into control group and observation group,and 45 people in each group.Children in control group were given Pan Li ketone tablet and the observation group were given four grinding soup oral liquid to observe the clinical curative effect of the two groups and gastric emptying function,symptoms and plasma substance P,motilin levels of patients before and after treating.Results The clinical efficient of Pan Li ketone tablet and four grinding soup oral liquid were 70.0％ and 94.0％ respectively.The total effective rate of tgastric emptying of the control group and observation group of were 74.0％ and 80.0％,and there were no significant difference (P ＞ 0.10).The plasma substance P,and motilin content of the two groups before and after treatment had significant difference(P ＜ 0.05),but there was no significant difference (P ＞ 0.05) between group after treating; Conclusion Four Grinding Soup oral liquid is a safe and effective way in treating pediatric indigestion,and it can promote gastric emptying function,plasma substance P and motilin levels,which provides certain reference significance for treating clinical indigestion.

For transplantation-eligible multiple myeloma patients,induction treatment of triple drug regimens followed by the collection of hematopoietic stem cells is generally recommended.For transplantationeligible patients,high-dose melphalan remains the standard regimen.Consolidation after transplantation has shown an improvement in response rates.Maintenance therapy with bortezomib or lenalidomide (or both in very-high-risk patients) is a reasonable option for long-term disease control and improvement in overall survival.Application of new agents into therapy of multiple myeloma should result in improved outcomes and long-term disease control.

Objective To explore the status quo of intensive statin therapy in hospitalized patients with acute myocardial infarction (AMI) from 2001 to 2011 and analyze its influence factors.Methods We obtained in-formation of AMI patients in 2001,2006,and 2011 from their medical record. Intensive statin therapy was defined as statin regiments with expected LDL-C lowering of at least 40%.Results Among 767 patientswith definite dosage of statin,the use frequencyof intensive statin increased from 0%in 2001 to 60.77% in 2006,and 88.71% in 2011 (P<0.0001).Morefemalesreceived intensive statin therapy than males(P=0.015)and the use frequency of intensive statin therapy in smoking patients was obviously more frequent than that in hypertension ,diabetes mellitus and hy-perlipidemia patients (P=0.0081).The use frequency of intensive statin therapy in patients with ischemic stroke was significantly lower than that in those with AMI or coronary artery disease(P=0.0437). AMI patients with STele-vatedwere much less likely received intensive statin therapy than those with non-ST elevated(P<0.0001). Chest pain,cardiac shock or cardiac arrest and the base level of LDL-C exerted no influence on the use of intensive statin therapy(P>0.05). Conclusions The use frequency of intensive stain therapy among AMI patientsincreasesyear by year and it is affected by sex,risk factors,history of disease and type of infarction.

This article briefly discussed the academic thoughts and clinical experience of Professor WANG Yan-gang in treating the stomach cold from the theory of Zhuodu. According to the theory of Zhuodu, Professor WANG Yan-gang usesHuazhuo Jiedumethod to relieve or significantly ease the symptoms of stomach cold by exploring the reasons through syndrome differentiation and treatment based on reason.

Objective To explore the difference of the results between pup smear and liquid-based cytology (TCT) in cervical lesions.MethodsA total of 150 patients undergoing routine cytology were enrolled in the department of obstetrics and gynecology in Hangzhou Wuyunshan sanatorium from May 2015 to December 2016.The patients were treated with liquid-based cytology (TCT) and Papanicolaou Smear examination;and liquid-based cytology method as the study group, and Pap smear the control group.The difference of the diagnostic results between the two groups were compared.ResultsThe positive rate of ASCUS detection, LSIL detection rate and HSIL detection rate were significantly higher in the study group than those in the control group (P<0.05).The difference between the two groups was statistically significant (P<0.05).Compared with the results of colposcopic biopsy, the diagnosis of LSIL coincidence rate in the study group was significantly higher than that in the control group, the difference between the groups was statistically significant (P<0.05).ConclusionCompared with pap smear examination, liquid-based cytology has a more obvious advantage in cervical lesions, which can significantly improve the positive rate of cervical lesions, reduce the false negative results, with a higher diagnostic compliance rate, and therefore worthy of clinical application.

Objective To observe clinical efrect of treating angina pectoris with Shuxuening injection combined with western medicine.Methods 62 cases patients with angina pectoris were randomly recurited into a control group and a treatment group,The control group was treated with western medicine,and the treatment group was treated with Shuxuening injection based on the control group.The clinical effect was observed between the two groups.Results The clinical effect in the treatment group(83.9%)was significantly higher as compared to the control group(64.5%)(χ~2=2.352,P＜0.05).Conclusion The treatment of Shuxuening injection combined with western medicine on angina pectods is better than westem medicine only.

Objective:To construct the eukaryotic expression plasmid expressing shRNA targeting EGFR gene family as a tool for following experiments in vitro and in vivo.Methods:EFGR family highly expressed in low radiosensitive ovarian cancer cells SKOV3,chosen highly expressed homologous genes in EFGR family as targeted gene.According to guidelines for shRNA design,designed experimental shRNA and negative control shRNA.In vitro synthesized 2 of the coding sequence of short hairpin RNA DNA single-strands,annealing,and cloned into the corresponding vector pGensil-1 of pol Ⅲ promoter downstream,named pGensil-1-E and pGensil-1-C.Confirmed by restrict endonuclease digestion and DNA sequencing.Conclusions:Eukaryotic expression plasmid experssing shRNA targeting homologous genes in EFGR family was constructed successfully.

Objective:To investigate the feedback regulation of transforming growth factor ?(TGF-?)to tumor necrosis factor-? converting enzyme.Methods:Reverse transcription polymerase chain reaction(RT-PCR)and immunochemistry were applied to detect TACE mRNA and protein in mice endometria of experiment group(TGF-? antibody was injected)and control group(saline was injected).Results:The expressions of TACE mRNA and protein in control group were higher than in experiment group.Conclusion: TGF-? could feedback on TACE expression in mice endometria and TACE-TGF-?-EGFR might one of the regulations during blastocyst implantation.

WNT signaling has been identified as one of the key signaling pathways in cancer,regulating cell growth,motility and differentiation.Because of its widespread activation in diverse human tumor diseases,the WNT pathway has gained considerable and growing interest in tumor research in recent years.