The periodontal regeneration is based on the proliferation and mult-differentiation of periodontal ligament cells.Thus,to find a method of enhancing the differentiation of periodontal ligament cells has been a hot point in studying periodontal disease.Many bioactive factors have important effects on the periodontal regeneration. Growth factors as bioactive factors have promotion effects on migration,growth,proliferation,differentiation,and protein synthesis.

Objective To observe and explore the clinical curative effect and mechanism of four grinding soup oral liquid in treating pediatric indigestion.Methods 90 children with indigestion were randomly divided into control group and observation group,and 45 people in each group.Children in control group were given Pan Li ketone tablet and the observation group were given four grinding soup oral liquid to observe the clinical curative effect of the two groups and gastric emptying function,symptoms and plasma substance P,motilin levels of patients before and after treating.Results The clinical efficient of Pan Li ketone tablet and four grinding soup oral liquid were 70.0％ and 94.0％ respectively.The total effective rate of tgastric emptying of the control group and observation group of were 74.0％ and 80.0％,and there were no significant difference (P ＞ 0.10).The plasma substance P,and motilin content of the two groups before and after treatment had significant difference(P ＜ 0.05),but there was no significant difference (P ＞ 0.05) between group after treating; Conclusion Four Grinding Soup oral liquid is a safe and effective way in treating pediatric indigestion,and it can promote gastric emptying function,plasma substance P and motilin levels,which provides certain reference significance for treating clinical indigestion.

Recent researches have achieved new progress in the function and signal transduction molecular mechanisms of erythropoietin (EPO).Previous studies confirmed that EPO is an independent angiogenic factor,especially in the development of retinopathy of prematurity (ROP).Recent research found that EPO protect the neurons as a neurotrophic factor.However,in proliferation stage as an angiogenic factor,EPO shares similarities with vascular endothelial growth factor ( VEGF ) in the early phase of diabetes.Besides,a recent study about effects of intravitreal EPO therapy for patients with chronic and progressive diabetic macular edema showed a short-term positive response.Here,the effects and molecular mechanisms of EPO/EPOR system in ischemic retinal disease were reviewed.

Ultrasound,MRI and mammography play significant roles in diagnosis,staging and follow-up of patients with breast cancer.With the development of individualized treatment of breast cancer,the molecular classification of breast cancer has vital reference value for treatment protocols.The requirements of medical imaging evolve from detecting breast cancer by morphological characteristics to making more accurate diagnosis using functional imaging for breast cancer.Correlations between molecular subtypes and ultrasonic,mammographic and MRI features of breast cancer attract broad attention.The correlation of molecular subtypes with uhrasound,MRI and mammography features in breast cancer patients were reviewed in this article.

Objective To explore the status quo of intensive statin therapy in hospitalized patients with acute myocardial infarction (AMI) from 2001 to 2011 and analyze its influence factors.Methods We obtained in-formation of AMI patients in 2001,2006,and 2011 from their medical record. Intensive statin therapy was defined as statin regiments with expected LDL-C lowering of at least 40%.Results Among 767 patientswith definite dosage of statin,the use frequencyof intensive statin increased from 0%in 2001 to 60.77% in 2006,and 88.71% in 2011 (P<0.0001).Morefemalesreceived intensive statin therapy than males(P=0.015)and the use frequency of intensive statin therapy in smoking patients was obviously more frequent than that in hypertension ,diabetes mellitus and hy-perlipidemia patients (P=0.0081).The use frequency of intensive statin therapy in patients with ischemic stroke was significantly lower than that in those with AMI or coronary artery disease(P=0.0437). AMI patients with STele-vatedwere much less likely received intensive statin therapy than those with non-ST elevated(P<0.0001). Chest pain,cardiac shock or cardiac arrest and the base level of LDL-C exerted no influence on the use of intensive statin therapy(P>0.05). Conclusions The use frequency of intensive stain therapy among AMI patientsincreasesyear by year and it is affected by sex,risk factors,history of disease and type of infarction.

This article briefly discussed the academic thoughts and clinical experience of Professor WANG Yan-gang in treating the stomach cold from the theory of Zhuodu. According to the theory of Zhuodu, Professor WANG Yan-gang usesHuazhuo Jiedumethod to relieve or significantly ease the symptoms of stomach cold by exploring the reasons through syndrome differentiation and treatment based on reason.

Objective To establish a method for the dissolution determination of Boling dripping pills.Methods With 0.5 % sodium dodecylsulfate as solvents and rotation speed being 100 r/min,a RP-HPLC method was used to deter- mine the content of herpetin with the detection wavelength of 280 nm.Results Herpetin showed a good linear relationship with peak area in the range of 0.084～1.008?g(r=0.9999),and the average recovery was 99.18% and RSD was 2.15 %.The dissolution of three batches was 87.3 %～90.8 %.Conclusion This method is simple,accurate,reliable and suitable for the quality control of Boling dripping pills.

Objective:To construct the eukaryotic expression plasmid expressing shRNA targeting EGFR gene family as a tool for following experiments in vitro and in vivo.Methods:EFGR family highly expressed in low radiosensitive ovarian cancer cells SKOV3,chosen highly expressed homologous genes in EFGR family as targeted gene.According to guidelines for shRNA design,designed experimental shRNA and negative control shRNA.In vitro synthesized 2 of the coding sequence of short hairpin RNA DNA single-strands,annealing,and cloned into the corresponding vector pGensil-1 of pol Ⅲ promoter downstream,named pGensil-1-E and pGensil-1-C.Confirmed by restrict endonuclease digestion and DNA sequencing.Conclusions:Eukaryotic expression plasmid experssing shRNA targeting homologous genes in EFGR family was constructed successfully.

physiological criteria of seaman in hot enviroment on J506 ship during the voyage on the tropics was investigated in May, 1980. It was found that the 6 physiological heat criteria(body temperature, pulse rate, electrocardiogram, 3 hours-sweat, urinary salt and the quantity of 24 hours-urine)were within the tolerable limits at the ordinary works, and the cabin temperature was not more than 32℃ (WBGT).The body temperature and pulse rate elevated when the workload increased.When the ship transfer from the high latitudes to the low latitudes in a short time,it is advised to a heat acclimatization, otherwise, the heat damags even heat stroke would occur.The water and electrolytes of the seaman was maintained in balance on the voyage. No special supplement was needed.

The toxic effect of boron on Langerhans' islands was studied with insulin radio-immunoassay.The results were as follows : the blood sugar decreased and the level of blood insulin increased in the experimental group (taking boron 30mg/kg body weight per day for 96 days),compared to those in the control group,and a deviating phenomenon of excretion curves occurred.The results suggest that boron may have a toxic effect on ?-cells of Langerhans islands,which leads to an increase in blood insulin and a decrease in blood sugar,and Langerhans' islands may be relatively sensitive target organs.This study will be of some value in making the criterion of boron concentration in drinking water for short term consumption of troops in war.