Objective To establish a method for the dissolution determination of Boling dripping pills.Methods With 0.5 % sodium dodecylsulfate as solvents and rotation speed being 100 r/min,a RP-HPLC method was used to deter- mine the content of herpetin with the detection wavelength of 280 nm.Results Herpetin showed a good linear relationship with peak area in the range of 0.084～1.008?g(r=0.9999),and the average recovery was 99.18% and RSD was 2.15 %.The dissolution of three batches was 87.3 %～90.8 %.Conclusion This method is simple,accurate,reliable and suitable for the quality control of Boling dripping pills.

Objective:To construct the eukaryotic expression plasmid expressing shRNA targeting EGFR gene family as a tool for following experiments in vitro and in vivo.Methods:EFGR family highly expressed in low radiosensitive ovarian cancer cells SKOV3,chosen highly expressed homologous genes in EFGR family as targeted gene.According to guidelines for shRNA design,designed experimental shRNA and negative control shRNA.In vitro synthesized 2 of the coding sequence of short hairpin RNA DNA single-strands,annealing,and cloned into the corresponding vector pGensil-1 of pol Ⅲ promoter downstream,named pGensil-1-E and pGensil-1-C.Confirmed by restrict endonuclease digestion and DNA sequencing.Conclusions:Eukaryotic expression plasmid experssing shRNA targeting homologous genes in EFGR family was constructed successfully.

Objective:To investigate the feedback regulation of transforming growth factor ?(TGF-?)to tumor necrosis factor-? converting enzyme.Methods:Reverse transcription polymerase chain reaction(RT-PCR)and immunochemistry were applied to detect TACE mRNA and protein in mice endometria of experiment group(TGF-? antibody was injected)and control group(saline was injected).Results:The expressions of TACE mRNA and protein in control group were higher than in experiment group.Conclusion: TGF-? could feedback on TACE expression in mice endometria and TACE-TGF-?-EGFR might one of the regulations during blastocyst implantation.

WNT signaling has been identified as one of the key signaling pathways in cancer,regulating cell growth,motility and differentiation.Because of its widespread activation in diverse human tumor diseases,the WNT pathway has gained considerable and growing interest in tumor research in recent years.

The plasticity of bone marrow stem cells is an important discovery in the field of stem cell research, which provides the possibility of bone marrow stem cells to be used for non-hematopoietic system diseases. There are rich data on bone marrow stem cells,which greatly accelerates the research on bone marrow stem cells plasticity. This article reviews the hot topics on plasticity of bone marrow stem cells.

physiological criteria of seaman in hot enviroment on J506 ship during the voyage on the tropics was investigated in May, 1980. It was found that the 6 physiological heat criteria(body temperature, pulse rate, electrocardiogram, 3 hours-sweat, urinary salt and the quantity of 24 hours-urine)were within the tolerable limits at the ordinary works, and the cabin temperature was not more than 32℃ (WBGT).The body temperature and pulse rate elevated when the workload increased.When the ship transfer from the high latitudes to the low latitudes in a short time,it is advised to a heat acclimatization, otherwise, the heat damags even heat stroke would occur.The water and electrolytes of the seaman was maintained in balance on the voyage. No special supplement was needed.

The toxic effect of boron on Langerhans' islands was studied with insulin radio-immunoassay.The results were as follows : the blood sugar decreased and the level of blood insulin increased in the experimental group (taking boron 30mg/kg body weight per day for 96 days),compared to those in the control group,and a deviating phenomenon of excretion curves occurred.The results suggest that boron may have a toxic effect on ?-cells of Langerhans islands,which leads to an increase in blood insulin and a decrease in blood sugar,and Langerhans' islands may be relatively sensitive target organs.This study will be of some value in making the criterion of boron concentration in drinking water for short term consumption of troops in war.

Objective To observe and explore the clinical curative effect and mechanism of four grinding soup oral liquid in treating pediatric indigestion.Methods 90 children with indigestion were randomly divided into control group and observation group,and 45 people in each group.Children in control group were given Pan Li ketone tablet and the observation group were given four grinding soup oral liquid to observe the clinical curative effect of the two groups and gastric emptying function,symptoms and plasma substance P,motilin levels of patients before and after treating.Results The clinical efficient of Pan Li ketone tablet and four grinding soup oral liquid were 70.0％ and 94.0％ respectively.The total effective rate of tgastric emptying of the control group and observation group of were 74.0％ and 80.0％,and there were no significant difference (P ＞ 0.10).The plasma substance P,and motilin content of the two groups before and after treatment had significant difference(P ＜ 0.05),but there was no significant difference (P ＞ 0.05) between group after treating; Conclusion Four Grinding Soup oral liquid is a safe and effective way in treating pediatric indigestion,and it can promote gastric emptying function,plasma substance P and motilin levels,which provides certain reference significance for treating clinical indigestion.

Allogeneic stem cell transplantation (allo-HSCT) is probably the only treatment for multiple myeloma (MM) with a curative potential.However,the application of allo-HSCT is limited by the high transplant-related mortality (TRM).Although the introduction of reduced intensity conditioning (RIC) has lowered the TRM associated with myeloablative conditioning,there is no improvement in survival of allo-RIC compared to autologous stem cell transplantation (auto-HSCT).New strategies are discussed with the aim of lowering transplant toxicity and boosting the graft-versus-myeloma effect,and these are urgently needed to make allo-RIC safer and more effective for myeloma patients.In this review,results from studies of allo-HSCT in MM are presented.

Up to now,one transketolase (TKT) and two transketolase-like genes (TKTL1 and TKTL2) have been identified in the human genome.People have conducted a series of basic and clinical researches of TKTL1 in malignant neoplasms since Coy found TKTL1 has a high expression of protein and gene levels in the malignant tumors in 2005.Although the biochemical and functional mechanism have not been clarified clearly,a positive role of TKTL1 in promoting tumorigenesis and tumor progression has been proved.