Objective To explore the relationship of body mass index ( BMI ) and atherosclerosis,blood pressure( BP) in patients with maintenance hemodialysis(MHD),to further elucidate the pathogenesis of cardiovascular disease in MHD patients.Methods The relationship between BP,intima-medial thickness (IMT) and BMI was analyzed in 101 patients with MHD.Results After dialysis,38％ patients had systolic blood pressure over 150mm Hg,21％ had diastolic blood pressure of more than 90mm Hg.Contrary to the general population,the correlation between BP and BMI were negatively (r =-0.13,P ＜ 0.01 ).Hypertension in patients with BMI ＜ 20 were more than that in patients with a normal body weight ( BMI 20.0 ～ 27.5 ) and overweight patients( BML ＞ 27.5 ),and the rate was 35％,29％ and 20％,respectively.Univariate analysis showed that BP and BMI were negatively correlated(r =-0.12,P ＜0.01),IMT was positively correlated with BMI(r=0.411,P ＜0.05).Conclusion In persistent hemodialysis patients,malnutrition was correlated with the occurrence and development of atherosclerosis and hypertension,and BMI decreasing was an independent risk factor,which could increase the morbidity and mortslity of cardiovascular disease.

Objective To investigate the cesarean section and repeat cesarean section on maternal and perinatal outcome.Methods 150 cases of repeat cesarean section cesarean section according to the number of cesarean section were divided into repeat cesarean section group(Group A)70 cases and primary cesarean section group (Group B)80 cases.The clinical data,pregnant women in general,intrapartum and postpartum complications,perinatal outcome in two groups were analyzed.Results The pregnancy with complicatiom in group A were significantly higher than that in group B(χ2=3.752,P＜0.05);The incidence of pelvic adhesions 50%in group A were significantly higher than that 23.8%in group B(χ2=7.852,P＜0.01);The breast-feeding in group A after 6 months,12months were lower than that in group B(χ2=1.6657;3.756,all P＜0.05).The incidence of pregnancy complications associated with the frequency of cesarean section(r=0.548,P＜0.05).Conclusion Repeat cesarean section does not affect the perinatal outcome,the incidence of pregnancy complications related to the number of cesarean section.

Objective:To investigate the magnetic resonance imaging(MRI) findings of spinal cord between neuromyelitis optica(NMO),and multiple sclerosis(MS).Methods:Subjects who were diagnosed or suspected as NMO or MS in the hospital since 2004 underwent free MRI(1.5T)scanning and imaging analysis of the brain,spinal cord and eye free.with reference to magnetic records,the results of cerebrospinal fluid(CSF)and visual evoked potential(VEP),patients who fulfilled the diagnostic criteria for the latest diagnostic criteria for NMO or McDonald Criteria for MS were enrolled and the follow-up study were carried out.Results:41 patients with NMO and 121patients with MS were included in this study.As to spinal cord injury,In NMO,most patients’number of affected segments was more than 5(78.1%),and most of the patients suffered either cervical spinal cord injury or cervical-thoracic spinal cord joint injury(80.5%).The lesions changed continually,and the spinal cord swelled obviously in acute phase,with white and gray matter of spinal cord injured simultaneously.For most MS patients,the thoracic spinal injury was frequent(46.6%).There were 41 patients(59.4%)whose affected segments were more than 3,but the affection was discontinuous and progressive,and the majority was white matter injury.There was statistical difference in the affected segments between the two groups(P

Sustaining survival of tumor cells is the main limiting step of tumor metastasis. Similar to tumor microenvironment, local environment in premetastatic organ (premetastatic niche) also provides a favorable condition for surviving of tumor cell and is the premise of tumor cell survival and proliferation in distant organ. Myeloid-derived suppressor cells (MDSCs) are the key component of the niche and play an important role in tumor cell implantation and metastasis formation through fabricating a proliferative, inflammatory, immunosuppressive and vascular remodeling premetastatic niche. MDSCs may also serve as potential therapeutic target for anti-metastasis therapy. Here, this study focused on the mechanisms of MDSCs in prematastatic niche formation and associated signal pathway, moreover, pro-vided reference to study on anti-metastasis therapy through interfering with the formation of premetastatic niche.

Based on the subtle discussion of changing rules and its principles of Six Qi movement recorded by one chapter named Liu Wei Zhi Da Lun in the Huang Di Nei Jing Su Wen about rules of Heaven divided by Six, Six Qi and year evolutive phase, Six Qi dominating the time, and guest climatic Qi adding to fixed host Qi, etc, this article explained the changes of influence of Heaven space-time on the human body from the angle of image thinking, focused on the key point of humanity conforming to the nature of the core of Chinese philosophy, and appealed readers to come back to the original thinking of China-image thinking.

Objective To detect the expression level of stage-specific genes in Leishmania promastigotes and amastigotes. Methods Total RNAs were isolated from Leishmania amazonensis stationary promastigotes and three sources of amastigotes: freshly obtained from mouse skin lesions,infected J774.G8 macrophages,and transformed from the cultured promastigotes. mRNAs were conversely transcribed into cDNA with SuperScripII reverse transcriptase and oligo dT primers. The polymerase chain reaction(PCR)was used to amplify the specific fragments of amastigote-specific nuclease(P-4)and promastigote-specific membrane glycoprotein(GP-46). PCR products were analyzed in 1.5% agarose gel. Results A P-4-specific band(273 bp)was observed in all three types of amastigotes with similar density,but it was almost undetectable in promastigotes. In contract,a GP-46-specific band(325 bp)was expressed at a higher level in promastigotes than in all three types of amastigotes. Conclusion Promastigote-derived amastigotes express high level of P-4-specific gene and can be used as a source of amastigotes for biochemical and immunological studies.

Objective:To explore the information technology to improve the efficiency of information sharing platform for obsolete and eliminated medical equipment.Methods: To design and develop a information sharing platform for obsolete medical equipment. This platform included platform construction, function introduction and application effect based on information characteristics of obsolete and elimination medical equipment, and the key point of information sharing. And to explore the next development direction for this platform.Results:This platform was based on internet technology and it can be used to share relevant information of obsolete and elimination medical equipment in a certain region. This platform included obsolete equipment inquiry, disposal information announcement and service obtaining and so on.Conclusion: The successful application of this platform standardize the management system to obsolete and dispose medical equipment. And it can unify the operation process and improve work efficiency. At the same time, it is a powerful management tool for relevant department and it can achieve preservation and increment of national asset in medical institutions.

BACKGROUND:Fibroblast growth factor can promote proliferation of mesenchymal stem cells (MSCs).grew along the wall.However,there are few reports on the differentiation of MSCs into hepatocytes following fibroblast growth factor induction.When mass concentration of hepatocyte growth factor was 1 μg/L,it can promote mitosis of hepatocytes,and is the strongest mitogenic agent for normal hepatocytes.OBJECTIVE:To explore the biological characteristics of human umbilical cord MSCs/n vitro and their differentiation ability to hepatocyte-like cells under the induction of chemical factors.DESIGN,TIME AND SETTING:The cytological in vitro study was conducted at the Institute of Blood,Jinan University from August 2008 to April 2009.MATERIALS:Umbilical cord was obtained from healthy full-term fetus,which was provided by the Guangzhou Huaqiao Hospital.The parturient signed informed consent.Hepatocyte growth factor and flbroblast growth factor were bought from Peprotech,USA.METHODS:The MSCs from human cord were isolated and cultured by type Ⅳ colagenase digestion+differential adherence.At the third passage,MSCs received cell surface antigen analysis and cell cycle determination to detect their ability to differentiate into adipocytes and osteoblasts.At the fifth passage,MSCs were adjusted into 5×10~9/L and assigned into 2 groups.BMSCs in the control group were incubated in DMEM/F12 containing 5% fetal bovine serum.BMSCs in the induction group were treated with above-mentioned medium supplemented with 20 μg/L hepatocyte growth factor and 10 μg/L flbroblast growth factor.MAIN OUTCOME MEASURES:The following parameters were measured:biological characteristics of human umbilical cord MSCs and differentiation of human umbilical cord MSCs into hepatocyte-like cells in vitro.RESULTS:At the third passage,MSCs derived from human cord expressed CD29,CD44,CD105,but not antigens of hematopoietic CD34,CD45,and 92.2% of them were in G_0/G_1 phase.Oil red O staining showed cytoplasm presented red granules.Alkaline phosphatase staining demonstrated that cytoplasm was black,with the differentiation ability into adipocytes and osteoblasts.Following 10 days of combined induction of hepstocyte growth factor and fibroblast growth factor,RT-PCR and Western blot results confirmed that cells expressed alpha fetoprotein and albumin.Negative expression was found in the control group.CONCLUSION:Human umbilical cord contained plenty of MSCs,with strong potential of multi-differentiation.Umbilical cord MSCs can differentiate into hepatocyte-like cells following combined induction of hepatocyte growth factor and fibroblast growth factor.

Spleen deficiency and phlegm dampness syndrome is the common syndrome of the chest blockage.In this article,the pathological characteristics,syndrome characteristics and treating principle of the spleen deficiency and phlegm dampness syndrome of chest blockage were discussed.

Objective With Dongcheng District residents for research, we used questionnaires, personal interviews, expert evaluation, systems analysis to study the health education mode of traditional Chinese medicine, and provide overall planning, pilot, summary of adjusted step by step implementation of final evaluation. In the adoption of a three-year health research and practice of traditional Chinese medicine, we have achieved good results. Area of traditional Chinese medicine culture has been strengthened, traditional Chinese medicine theory and appropriate technology have been widely used in the resident wellness activities. An appropriate health education mode of health education of traditional Chinese medicine is significant to highlight features of traditional Chinese medicine.