Objective To evaluate the use of tryptase in diagnosing allergic diseases.Methods Serum tryptase was measured by Unicap 100 in 35 patients with different types and states of allergic diseases, and 30 healthy volunteers as controls ;Serum tryptases were compared with IgE level.Results Among the 35 allergic patients, 18 cases were with chronic urticaria(4.74?2.54)?g/L; 10 cases had past histories of anaphylaxis shocks(4.86?2.55)?g/L ;7 cases were in acute phase of anaphylaxis(17.4?7.87)?g/L ;30 healthy volunteers(4.36?1.67)?g/L. A statistically significant increase in tryptase concentration was found in patients with acute phase of anaphylaxis compared with other groups.No correlation was found between tryptase concentration and total IgE level.Conclusions Measurement of Serum tryptase level could be an additional tool for diagnosis of anaphylaxis but not chronic urticaria.

The independent component analysis is a new method for signal processing, which has been paid attention to in the field of biomedical engineering. This paper introduces the design of biopotential analysis system based on ICA neural network and discusses its foundamental components.

Objective: To investigate the effect of Nano-Se with lower toxicity on the anti-tumor effect of cisplatin. Method: For toxicity comparison, mice were orally administered Nano-Se and sodium selenite to observe death, body weight loss and liver injury. As to the protective effect of Nano-Se on the toxicity of cisplatin, mice with and without Nano-Se supplementation were injected cisplatin to observe death and body weight loss. For evaluation of whether Nano-Se influence the anti-tumor effect of cisplatin, Lewis lung carcinoma was transplanted into mice, and the survivor and tumor volume were recorded to compare the effect of Nano-Se supplementation on cisplatin. Results: The toxicity of Nano-Se is significantly lower than sodium selenite. Nano-Se can decrease toxicity of cisplatin without compromising its anti-tumor effect. Conclusion: Nano-Se with lower toxicity can improve the therapeutic effect of cisplatin.

OBJECTIVE To investigate the bacteriologic feature and the spectrum of drug resistance in lung cancer patients who got nosocomial nonfermenters bacteria infection,and to conclude the experience and how to prevent and control the nosocomial infection.METHODS A rectrospective analysis of the composition of the pathogens,the feature of drug resistance and the prognosis of 109 lung cancer inpatients with nosocomial infection was made.RESULTS Nosocomial nonfermenters bacteria infections were most happened in hospital lung cancer patients.Main strains included Pseudomonas aeruginosa 55(49.5%),Acinetobacter baumannii 23(20.7%),and Stenotrophomonas maltophilia 8(7.2%).The sensitive rate of P.aeruginosa to imipenem was 100%,but to Cotrimoxazole,the resistant rate was 100%.The sensitive rate was relatively high to ticarcillin,piperacillin and their compounds.The antimicrobial resistance rate of A.baumannii was higher than P.aeruginosa.Stenotrophomonas were resistant to most antibiotics,and the resistance rate to imipenem was 100.0%.CONCLUSIONS The resistance rate of nosocomial nonfermenters bacteria infection is high.The treatment is difficult and the prognosis is bad.The mortality of multiple infection is high.

Objective To describe and explore the practice of eye care in ICU,in order to make a specified training plan and guideline on eye care,and to improve the skill in eye care of ICU nurses. Methods A survey was conducted in 92 ICU nurses from 2 general hospitals in Huizhou city with non-random sampling using a self- made questionnaire to investigate their eye care of patients. Results The nursing practice of eye care in ICU was urged to be improved,there was no standard on eye care. Conclusions The knowledge of ICU nurses needs to be enhanced,and guideline on eye care is urged to be worked out,so that practice of eye care would be improved.

Objective To explore the expression and significance of chemokine CXC reeeptor (CXCR)3 and CXCR4 and their ligands(CXCL)at the early pregnancy decidua and villi.Methods Decidual mononuclear cells were isolated from the normal decidua of 5-8 weeks pregnant women by lymphocyte separation medium in vitro.CD56+natural killer(NK)cells were purified by dynabeads cell sorter kiL Purity and phenotype of CD56+decidua NK cells were analyzed by fluorescence-activated eell sorter (FACS).Gene expression of CXCR3 and CXCR4 in decidua NK cells and CXCL9,CXCL10 and CXCL12 in early pregnancy decidua and villi was assessed bv RT.PCIZ Protein expression of CXCL9,CXCL10 in normal endometrium and early pregnancy decidua was characterized and quantified by streptavidin-biotin pemxidase chain reaction(SP)immunohistochemistry and computered image analysis system.Correlations between the gray degree of CXCL9 and CXCL10 and the number of CD56+NK cells in upper tissue were analyzed by Spearman's correlation ceefficient rank tesL Results The phenotype of 98.7%decidua NK cells was CD56bright.The genes of CXCR3 and CXCR4 were expressed in decidua NK cells and that of CXCL9 and CXCL1O were expressed in early pregnancy decidua and CXCLI2 in early pregnancy villi.CXCL9 and CXCL10 were expressed in the cytoplasm of surface epithelia,glandular epithelia and stromal cells of early pregnancy deeidua and were not expressed in villi by immunohistochemistry.The gray degree of CXCL9 and CXCL10 in the secretory phase endometrium(56±43,59±47)was stronger than that in the proliferative phase(16±18,8±14,P<0.05)and reached the highest(143±35,158±29,P<0.05)in the early pregnancy decidua.The number of cD+56 NK cell in the secretory phase endometrium(60±20)was more than that in the proliferative phase endometrium(23±4,P<0.05)and was the most in the early pregnancy decidua(114±15,P<0.05).The gray degree of CXCL9 in upper tissue had a positive correlation with the number of CD+56 cells(r=0.88,P<0.05)and that of CXCL10 had a similar pattern to CXCL9(r=0.86,P<0.05).Condusion The interactions between CXCL9,CXCL10 and CXCL12 expressed in decidua and villi and CXCR3,CXCR4 expressed in CD+56 decidua NK cells may influence the CD+56 NK cell recruitment at the maternal-fetal interface.

Objective To explore the relationship between quality of life and sub-health state of Guangdong urban dwellers.Methods A total of 2 200 urban dwellers were investigated using SF-36 and Sub-Heahh Measurement Scale Version 1.0(SHMS V1.0).Results As for the scores in PCS,MCS,psychosocial symptom,cognitive function,social adaptability,those of man urban dwellers (respectively (78.89± 15.25),(73.15 ± 15.26),(63.73± 18.82),(62.13± 19.34),(68.04± 17.90)) were higher than those of woman dwellers (respectively(76.98± 14.20),(71.76± 14.72),(60.53± 18.28),(57.00± 17.89),(65.73± 17.07)).The difference was statistically significant (P＜0.05).Bivariate correlation analysis showed that the PCS and MCS of quality of life were positively related with all dimensions of sub-health (P＜0.001).Canonical correlation analysis indicated that vitality,social adaptability,psychosocial symptom and positive emotion were the main factors of sub-health status,and MCS was of quality of life.Conclusion Sub-health state of Guangdong urban dwellers is positively related with quality of life,and the more serious sub-health state is,the worse quality of life is.Therefore,intervention of subhealth and quality of life should be targeted to be strengthened according to the investigation.

OBJECTIVE To evaluate the commonly encountered pathogens and drng resistance of bacteria causing secondary infection in patients with malignant tumors and provide reference for clinical antimicrobial usage.METHODS Statistical analysis was made retrospectively in the classification and antimicrobial susceptibility testing in 1 204 strains of pathogens isolated from clinical samples of hospitalized patients with malignant tumors from Jan 2003 to Oct 2005.RESULTS Among 1 204 strains,Gram-positive strains accounted for 31.1%;Gram-negative ones accounted for 52.8%;and fungi accounted for 16.1%.The principal strains were Candida albicans,Escherichia coli,Staphylococcus aureus,Klebsiella pneumoniae,and Pseudomonas aeruginosa.The most frequent infection sites were in respiratory tract,urinary tract and skin-soft tissue.Multiple drug resistant rate of Gram-positive and Gram-negative strains had a tendency of elevation.CONCLUSIONS To reduce the coming of drug resistant strains,etiologic examination should be done in treatment of infectious diseases and antimicrobial therapy should be decided according to the results of susceptibility.

To compare the chemical components of supercritical extracts from Herba Ephedrae and honey-prepared Herba Ephedrae. Volatile oils were extracted from Herba Ephedrae and honey-prepared Herba Ephedrae by supercritical CO 2 extraction and then identified by the combination of gas chromatography and mass spectrometry (GC-MS). Twenty kinds of components such as diethylsulfate obtained from Herba Ephedrae were not found in honey-prepared Herba Ephedrae and twelve different components such as hydroxymethylfurfural were obtained from honey-prepared Herba Ephedrae. The extraction rate of volatile oils was 2.1% in herba ephedrae and 1.0% in honey-prepared Herba Ephedrae. [ Conclusion]The components of supercritical extractions from Herba Ephedrae differ from those in honey-prepared Herba Ephedrae.

Objective The protein kinase C ( PKC) is an essential signaling substance in the early protection of cells in pre-conditioning.This study was to investigate the role of PKC in the myocardial cells of the rat model of anoxia -reoxygenation (A-R) in-jury preconditioned with sufentanil . Methods Primary myocardial cells isolated and cultured for 5 days were allocated to a control , an A-R, a sufentanil preconditioning ( SF) , and a phorbol+sufentanil preconditioning ( PMA +SF) group.The A-R injury model was established with cultured myocardial cells from neonatal rats , which were preconditioned with sufentanil at the concentration of 0.000 3 μmol/L in the SF group or phorbol followed by sufentanil 10 minutes later in the PMA +SF group.Then the proliferation of the cells was detected , the optical density of the Cx 43 protein observed by immunofluorescence confocal technology , the apoptosis rate of the cells determined by flow cytometry, and the total Cx43 proteins calculated by Western blot. Results Cell proliferation was signifi-cantly increased in the A-R, SF, and PMA+SF groups as compared with the control (P<0.05), higher in the SF and PMA +SF than in the A-R group (0.498 0 ±0.0432 4 and 0.7240 ±0.1234 vs 0.325 8 ±0.023 5, P<0.05), and in the SF than in the PMA+SF group (P<0.05).Flow cytometry showed significantly increased rates of early , late, and total cell apoptosis in the A -R ([4.96 ±0.59], [18.77 ±0.92], and [23.73 ±0.51]%), SF ([5.86 ±0.38], [10.37 ±0.38], and [16.23 ±0.32]%), and PMA+SF group ([5.71 ±0.58], [5.54 ±0.43], [11.24 ±0.62]%) as compared with the control (P<0.05), remarkably lower in the SF and PMA +SF than in the A-R group (P<0.05), and the late and total cell apoptosis rates markedly lower in the SF than in the PMA+SF group (P<0.05). Conclusion Sufentanil has a protective effect and PKC agonists combined with sufentanil may add to the effect on myocardial cells in A -R injury.