Periodontal ligament may have independent response to orthodontic stimulation and thus initiate alveolar bone osteogenesis and osteoclasts. Orthodontic-induced alveolar bone remodeling has no bone loss,while inflammation can lead to alveolar bone loss,suggesting that force signal and inflammatory factors may induce the differentiation of undifferentiated cells in the periodontal ligament via different pathways. The strength of the force and the nature of the force (basal tension and fluid shear force) may affect the differentiation of periodontal ligament cells,and may produce antagonistic or synergistic effect with the inflammatory factors through complex autocrine and paracrine regulation,resulting in local bone reconstruction,which is manifested as bone deposition and bone absorption. Studies have shown that Wnt signaling is an important regulatory pathway for osteoblast differentiation. Inflammatory factors can block the differentiation of stem cells into osteoblasts. The Wnt pathway is closely related to the effects of force and inflammatory factors on the differentiation of periodontal ligament cells.

Objective To investigate the relationship of carotid artery stenosis and early postoperative cerebrovascular accident in patients undergoing off-pump coronary artery bypass grafting.Methods One hundred patients with carotid artery stenosis were divided into two groups by random digits table method:off and on-pump coronary artery bypass grafting group.The incidence of early cerebral vascular accident after operation in two groups (48 h after operation) was compared and analyzed.Results The rate of early postoperative cerebral vascular accident in on-pump coronary artery bypass grafting group was 18.2％ (8/44),1 patient occurred cerebral infarction,7 patients occurred transient ischemic attack,none occurred cerebral hemorrhage.The rate of early postoperative cerebral vascular accident in off-pump coronary artery bypass grafting group was 5.4％(3/56).The rate of early postoperative cerebral vascular accident between two groups had significant difference (x2 =4.141,P =0.042).Conclusion The patients with carotid artery stenosis with off-pump coronary artery bypass grafting can reduce early postoperative cerebral vascular accident.

Objective To study the expression of Stathmin gene in ovarian cancer tissue and its valuation value on effect of Paclitaxel combined with cisplatin chemotherapy.Methods 92 cases of ovarian cancerpatients underwent surgical and postoperative chemotherapy treatment in our hospital during July 2012 to February 2016 were selected with research subject.The expression of Stathmin gene in ovarian cancer tissues and adjacent tissues was measured by fluorescence quantitative PCR.All ovarian cancer patients were divided into high Stathmin group and low Stathmin group with 46 cases according to the expression of Stathmin gene in ovarian cancer tissues.Comparison the chemotherapy effectin ovarian cancerpatients with different Stathmin gene expression.Results The expression of Stathmin mRNA in ovarian cancer tissues was significantly higher than that in adjacent tissues(P<0.05).After chemotherapy, serum tumor markers such as HE4, CA199, CA153, β-HCG in high Stathmin group were higher than those in low Stathmin group, angiogenesis indexes such as CXCR4, SDF-1, VEGF, Ang2 were lower than those in low Stathmin group, apoptotic indexes such as Fas/Apo-1 and Bcl-2 were higher than those in low Stathmin group(P<0.05).Conclusion Stathmin gene is highly expressed in ovarian cancer tissues, and the expression of Stathmin is negatively correlated with chemotherapy.

Objective To study the application value of CD10,desmin and vimentin in the diagnosis and differential diagnosis of special types of uterine tumors.Methods The clinical data of 79 cases of special types of uterine cancer were retrospectively analyzed.CD10,desmin and vimentin were detected respectively by immunohistochemical streptavidin-perosidase(SP) method.Results The positive rates of CD10 in endometrial stromal sarcoma,epithelioid leiomyoma,atypical leiomyoma,leiomyosarcoma,carcinosarcoma were 76.2%,0.0%,13.3%,30.8%,75.0%,respectively.The positive rates of vimentin in endometrial stromal sarcoma,epithelioid leiomyoma,atypical leiomyoma,leiomyosarcoma,carcinosarcoma were 100.0%,5.6%,0.0%,46.2%,83.3%,respectively.The positive rates of desmin in endometrial stromal sarcoma,epithelioid leiomyoma,atypical leiomyoma,leiomyosarcoma,carcinosarcoma were 0.0%,61.1%,53.3%,30.8%,0.0%,respectively.The positive rates of vimentin and CD10 in endometrial stromal sarcoma and carcinosarcoma were all significantly higher than those in atypical leiomyoma,epithelioid leiomyoma(CD10:χ2=23.255,13.829,15.880,8.102,all P=0.000;vimentin:χ2=35.159,36.000,15.556,16.440,all P=0.000).The positive rates of desmin in atypical leiomyoma,epithelioid leiomyoma tissues were significantly higher than those in endometrial interstitial sarcomas,carcinosarcoma(χ2=11.480,6.717,17.875,9.097,all P=0.000).Conclusion CD10,desmin and vimentin can be used as sensitive indicators for the differential diagnosis of uterine tumors with special types.

Objective To develop transgenic mice harboring the fusion gene of mutant amyloid precursor protein and two types of fluorescent protein for the future study on Alzheimer's disease.Methods The fusion gene CFP-54 bp-YFP-C99 was introduced into mice by mieroinjection.The presence of CFP-54 bp-YFP-C99 was confirmed by PCR in the founders.Results CFP-54 bp-YFP-C99 gene was injected into pronucleus of 2202 zygotes and 1806 injected eggs were implanted into 56 foster mothers, 13 of which were pregnant.There were 13 foster mothers who borne 52 offspring and 32 of them survived.Recipient mouse pregnancy rate was 23.2% (13/56) and the integration rate was 3.9% (2/52).Conclusion CFP-54 bp-YFP-C99 transgenic mice is obtained, but the transgenic efficiency is low.

Objective:To explore the effect of music relaxation training (MRT) in patients with breast cancer after axillary lymph node dissection (ALND) .Methods:A total of 72 breast cancer patients admitted to Qingdao Central Hospital and Qingdao Cancer Hospital from January 2019 to January 2021 were selected for control experiments. All patients were treated with ALND based on their specific conditions. According to the random number table method, the patients were divided into the control group and the intervention group, each with 36 cases. The control group carried out routine nursing, and the intervention group conducted MRT on the basis of routine nursing. The Short Form 36 Health Survey (SF-36) and Visual Analog Scale (VAS) scores of the two groups before treatment and at discharge were compared.Results:After the intervention, the SF-36 score and satisfaction of the intervention group were higher than those of the control group, and the VAS score was lower than that of the control group, and the differences were statistically significant ( P<0.05) . Conclusions:The application of MRT after ALND in breast cancer patients can improve the health of patients, increase patient satisfaction, and reduce their pain.

Objective: To explore the clinical applications of second generation colon capsule endoscopy (CCE-2). Methods: From July 2017 to December 2018, at the First Affiliated Hospital, College of Medicine, Zhejiang University, 40 outpatients and hospitalized patients who underwent CCE-2 examination were enrolled. The examination results were analyzed by an expert gastroenterologist with rich experience in small intestinal and colon capsule endoscopy. The stomach, small bowel and colon transit time, the score of colon cleansing quality, the completion rate of colon capsule examination, lesion detection and adverse effects were observed. Chi-square test and Student′t test were used for statistical analysis. Results: The whole gastrointestinal tract examination was completed during the capsule running time in 65.0% (26/40) of the patients. The average stomach transit time was (0.92±0.74) h, the small bowel transit time was (3.93±1.51) h and the colon transit time was (4.89±0.61) h. The capsule running time of patients who completed the whole gastrointestinal tract examination was shorter than that of patients who did not complete the whole gastrointestinal tract examination ((9.44 ± 3.53) h vs. (15.47±2.09) h), and the difference was statistically significant (t=6.79, P<0.01). The qualified rate of colon preparation was 67.5% (27/40). There were no statistically significant differences in colon transit time or capsule excretion time between patients with qualified colon preparation and poor colon preparation ((4.43±3.33) h vs. (5.96 ± 2.44) h; and (9.06 ± 3.91) h vs. (10.29±2.47) h; t=1.17 and 0.81, both P>0.05). A total of 33 (82.5%) patients had gastrointestinal lesions detected by colon capsule, including three cases of esophageal lesions (inflammation and mass), 21 cases of gastric lesions (chronic gastritis, mucosal protrusion, polyp and ulcer), nine cases of small bowel lesions (polyp, ulcer and vascular malformation) and 19 cases of colonic lesions (diverticulum, polyp, rectitis, mucosal erosion, ulcer and vascular malformation, internal hemorrhoids). Among them, there were 11 patients with two or more lesions. No adverse events occurred during the examination and all the capsules were excreted within 48 hours. Conclusion CCE-2 with high safety and good tolerance can be used for whole gastrointestinal tract examination.

Objective: To explore the relationship between depression, anxiety, stress and food addiction among primary and secondary school students in Yixing City, so as to provide reference and suggestions for maintaining healthy eating behavior and psychological health intervention and promotion among primary and secondary school students in Yixing City. Methods: From December 2022 to February 2023, a stratified cluster random sampling method was used to select 4 180 primary and secondary school students from four primary and secondary schools in Yixing City, Jiangsu Province. A questionnaire survey was conducted using the Depression Anxiety Stress Scale-21 (DASS-21) and related behavior questionnaires. The data was analyzed using χ 2 test, Wilcoxon, Kruskal Wallis H rank sum test, and binary Logistic regression. Results: The prevalence of food addiction among primary and secondary school students in Yixing City was 0.98% (41 students), and there was no statistically significant difference in the comparison of food addiction, depression, anxiety, and stress scores among students of different genders and age groups ( Z/H = -1.34- 5.74, P >0.05). There was a positive correlation between food addiction and binge eating behavior, depression, anxiety, and stress symptoms ( r=0.14-0.23, P <0.01). The results of binary Logistic regression showed that anxiety ( OR=5.68, 95%CI =1.74- 18.55 ) and stress ( OR=5.41, 95%CI =2.20-13.32) were positively correlated with the occurrence of food addiction in primary and secondary school students ( P <0.01). Conclusions The risk of food addiction among primary and secondary school students with anxiety and stress symptoms is higher than that of the general population. Guidance and intervention on student mental health should cover the entire compulsory education and high school period, in order to improve the mental health level of students and reduce the occurrence of food addiction behavior.

Objective To investigate the predictive value of a nomogram model constructed with clinical variables and multiparametric magnetic resonance imaging(MRI)radiomics features in prognosis of patients undergoing chemotherapy for breast cancer.Methods A total of 150 breast cancer patients who received chemotherapy in Qingdao Central Hospital from January 2020 to December 2021 were enrolled for the study.All patients underwent breast MRI examination before chemotherapy,and the optimal radiomics features were extracted from the tumor regions of interest on multiparametric MRI image which were manually outlined using segmentation software.After 2-year follow-up,the patients were divided into good prognosis group and poor prognosis group according to whether there were disease recurrence,metastasis and death,and they were divided into training set(n=105)and test set(n=45)in a ratio of 7:3.The clinical data and multiparametric MRI radiomics features were collected,and the factors affecting the prognosis of breast cancer patients receiving chemotherapy were analyzed by univariate and multivariate Logistic regression analyses.Three models,namely clinical model,radiomics model,and clinical-multiparametric MRI radiomics-based nomogram model were constructed,and their predictive value in the prognosis of breast cancer patients treated with chemotherapy was analyzed with receiver operating characteristic curves.Results Out of 150 cases of breast cancer treated with chemotherapy,52 cases were included in poor prognosis group,with 33 cases of recurrence and/or metastases and 19 cases of deaths.Logistic regression analysis identified TNM stage,estrogen receptor and progesterone receptor as clinical predictors for prognosis of breast cancer patients treated with chemotherapy(P<0.05).There were 69 and 36 patients with good and poor prognoses in training set,and 29 and 16 patients with good and poor prognoses in test set.Through least absolute shrinkage and selection operator algorithm,16 radiomics features related to the prognosis of breast cancer patients treated with chemotherapy were screened out from TIRM,DWI,and DCE-MRI sequences,and their radiomics scores were calculated.Compared with patients with poor prognosis,those with good prognosis in training set and test set had higher radiomics scores(P<0.05).ROC curve analysis showed that the area under the curve of radiomics model,clinical model,and nomogram model were 0.853,0.741,0.923 in training set(P<0.05),and 0.797,0.749,0.896 in test set(P<0.05),respectively.Conclusion Based on pre-chemotherapy radiomics scores,TNM stage,estrogen receptor and progesterone receptor are identified as independent predictors for prognosis of breast cancer patients treated with chemotherapy,and the clinical-multiparametric MRI radiomics-based nomogram can effectively improve the predictive efficacy of prognosis in breast cancer patients treated with chemotherapy.

Objective:To investigate the clinicopathological, immunohistochemical and molecular genetic characteristics of TFE3-rearranged perivascular epithelioid cell tumor (PEComa).Methods:Eight cases of PEComa with TFE3 rearrangement diagnosed in the First Affiliated Hospital of Air Force Medical University from January 2014 to July 2022 were collected. Three were consultation cases and 5 were collected from our hospital; 7 cases were resection specimens and 1 case was a needle biopsy specimen. Routine histolopathological analysis, immunohistochemical staining, fluorescence in situ hybridization (FISH) and the next-generation sequencing were performed. Clinical data were collected and the prognosis was assessed.Results:The 8 patients consisted of 5 females and 3 males with a median age of 45 years (ranged from 25 to 65 years). The tumor location included 1 uterus, 1 liver, 1 urachus, 2 kidneys, 1 abdominal cavity, 1 colon, and 1 retroperitoneum (3 subsequent recurrences in the abdominal cavity, pelvis and ovary, and abdominal cavity, respectively). Morphologically, the tumor cells were uniform and epithelioid with translucent or eosinophilic cytoplasm. They were arranged in nests or sheets, most of which were separated by thin-walled blood vessels. There were no papillary structures, and no overt smooth muscle or fat components. Atypical features were seen in 3 cases, with bizarre nuclei and tumor giant cells. Large areas of necrosis were visible, and mitosis was common (up to 28/50 HPF). Melanin deposition was present in 3 cases. Immunohistochemical staining showed diffuse and strong positivity for TFE3 in 8/8 cases and for HMB45 in 6/8 cases; focal positivity for Cathepsin K and Melan-A in 6/8 cases and for SMA in 2/8 of cases. All cases were negative for CKpan, PAX8 and Desmin. TFE3 gene break-apart was detected by FISH in all 8 cases, 4 of which underwent next-generation sequencing, and it revealed that 2 cases presented with SFPQ::TFE3 fusion, 1 case with ASPSCR1::TFE3 fusion, and 1 case with no chimeric fusion. Seven cases were followed up for 4—94 months. All cases were alive; 4 cases were disease-free, 2 cases showed recurrence, and 1 case had metastasis at initial diagnosis.Conclusions:TFE3-rearranged PEComa has unique histomorphological, immunohistochemical and molecular characteristics. The biological behavior is aggressive, which could lead to recurrence and metastasis, and warrants close clinical follow-up.