Objective To study the correlation of the changes of routine coagulation index and D-dimmer(D-D) with postpartum hemorrhage. Methods 37 patients with postpartum hemorrhage were divided into 2 groups ac-cording to the quantity of bleeding(group 1,27 patients, postpartum hemorrhage 500 ～ 800ml; group 2,10 patients, postpartum hemorrhage more than g00ml) and 30 patients with normal deliveries as control. Routine congulation in-dex, activated partial thromboplastin time(APIT) ,prothrombin time(PT) ,thrombin time(13") ,fibrinogen(Fg) and D-D were detected during the end of delivery and 48 hours after delivery. Results Compare with control group at the end of the delivery in group 1 ,the D-D was remarkably increased (P<0.05) ,but there was no significant difference of the routine coagulation index compared with control group (P > 0.05), in group 2, the D-D, PT, APTT were in-creased, whereas the Fg was decreased (P < 0.05). 48 hours after delivery, compared with control group,the D-D was significantly higher(P < 0.05), whereas the routine coagulation index had no significant difference (P > 0.05). The lowering of D-D, increase of Fg were higher in group 2 than those in group 1 (P < 0.05). Conclusion It has impor-tant clinical significance to timely monitor routine coagulation index and D-D at the end of delivery and after delivery for understanding the coagulation function of parturient and preventing disseminated intravascular coagulation.

In recent years,two important component proteins of the calcium release-activated calcium channel (CRAC) were identified fromDrosophila cells by RNA interference technique,including the calcitum sensor stromal interaction molecule 1 (STIM1) on the endoplasmic reticulum and the CRAC channel protein Orail on the cell membrane.Studies have shown that STIM 1 and Orail have regulatory effects on vascular smooth muscle cells,platelets,vascular endothelial cells and other cells.They play important roles in the aspects of vascular smooth muscle cell phenotypic modulation,hemostasis,thrombosis,and neovascularization.It shows that they both may be closely associated with ischemic cerebrovascular disease.This article reviews the advances in research on STIM1 and Orail proteins in ischemic cerebrovascular disease in order to investigate the possibility of STIM1/Orai1 as a new target in the prevention andtreatment of ischemic cerebrovascular

Resistin is a recently discovered polypeptide hormone.Studies have shown that resistin is closely associated with atherosclerosis by promoting inflammatory response and adhesion of vascular endothelial cells,transformation from macrophages to foam cells,vascular smooth muscle cell proliferation and migration,increasing plaque instability,as well as promoting carbohydrate and lipid metabolism disorders,etc.Resistin can promote the occurrence and development of ischemic cerebrovascular disease.It may become a potential biological marker and intervention target.

Objective To investigate the effect of acupoint injection of Xingnaojing injection on the intelligence development and hemorheology in children with mental retarded cerebral palsy.Methods Sixty patients with cerebral palsy complicated with mental retardation were selected from March 2015 to January 2017 in Anyang Maternal and Child Health Care Hospital.The patients were divided into observation group and control group according to the treatment methods,thirty cases in each group.The patients in the control group were treated with routine treatment and rehabilitation training;based on these,the patients in the observation group were treated with acupoint injection of Xingnaojing injection.The developmental quotient (DQ) was calculated by the children's neuropsychological development scale.The therapeutic effects,intelligence and haemorheology improvement of patients in the two groups were evaluated.Results There was no significant difference in DQ score between the two groups before treatment (t =0.545,P ＞ 0.05).The DQ score after treatment was significantly higher than that before treatment in the two groups (t =9.386,10.057;P ＜ 0.05).The DQ score in the observation group was significantly higher than that in the control group after treatment (t =3.641,P ＜ 0.05).There was no significant difference in hemorheological indexes between the two groups before treatment (P ＞ 0.05).The high shear whole blood viscosity,low shear whole blood viscosity,plasma viscosity,hematocrit,platelet adhesion rate and fibrinogen level after treatment were significantly lower than those before treatment in the two groups (P ＜ 0.05).The high shear whole blood viscosity,low shear whole blood viscosity,plasma viscosity,hematocrit,platelet adhesion rate and fibrinogen level in the observation group were significantly lower than those in the control group after treatment (P ＜ 0.05).The total effective rate in the control group and observation group was 66.67％ (20/30) and 90.00％ (27/30) respectively,the total effective rate in the observation group was significantly higher than that in the control group (x2 =4.812,P ＜ 0.05).Conclusion Acupoint injection of Xingnaojing injection can significantly improve the intelligence,hemorheology and therapeutic effect in children with cerebral palsy complicated with mental retardation.

Objective:To study the distribution of the literature and the trend of international research of proliferative vitreoretinopathy (PVR) in 10 years from 2009 to 2018.Methods:Using Web of Science database of the American Institute of Scientific Information as the data source to collect literature on proliferative vitreoretinopathy published from 2009 to 2018 and a statistical analysis on the distribution of the publication time, nation and funding agency was made to figure out the institution, periodical, author with highest citations respectively among the collected literature.Meanwhile, the bibliometric analysis software Citespace and VOSviewer were employed to carry out the distribution analysis and cluster analysis of keywords as well as the co-citation cluster analysis.Results:A total of 905 articles related to PVR were included, mainly in English, most of which were written by American authors, and the number of literature published by Chinese authors was ranked the second.Among all the article funding institutions, the National Natural Science Foundation of China ranked NO.1 with the most funded articles.Citations increased year by year since 2009 and increased obviously since 2013.The journal with the highest citations was Investigative Ophthalmology & Visual Science, and the research institution with the highest citations was Harvard University.The statistical analysis of the citation quantity also showed that the top five authors with the highest citations were all from Massachusetts Eye and Ear, Department of Ophthalmology, Harvard Medical School.The results of cluster analysis showed that there were 41 high-frequency subject terms, and they were mainly grouped in 4 categories, which were etiology, risk factors, molecular mechanism, treatment and management of PVR.Keywords network visualization and overlay visualization both showed that etiology and risk factors were the focus of current research, while epithelial mesenchymal transition, transforming growth factor-β and fibrosis were new research fields in recent years.The results of citation cluster analysis showed there were 12 co-citation clusters. Conclusions:The literature of PVR shows an upward trend, and the researches focus on the molecular mechanism, prevention, and neuroprotection of PVR, which may be the future PVR research direction.

Objective To study the correlation of the level of high-sensitivity C-reactive protein (Hs-CRP) in the acute phase of ischemic stroke and progressive stroke. Methods 101 patients with acute ischemic stroke were collected. Serum Hs-CRP of all patients were measured at 24 hours after admission , the onset of 48 hours, the onset of 72 hours by latex enhanced immune turbidity method. According to their serum Hs-CRP lev-els, the patients were divided into the group of Hs-CRP sustained growth (n = 35) and the group of non Hs-CRP sustained growth (n = 66). The NIHSS scores were assessed on two groups of patients , Logistic regression analysis was made to screen the related factors of Hs-CRP increase and the possible risky factors for progressive ischemic stroke. Results There was a significant difference of serum Hs-CRP level between the two groups (P <0.001). The incidence of progressive stroke in the group of Hs-CRP sustained growth was significantly higher than that in the group of non Hs-CRP sustained growth (χ2 = 32.710, P < 0.001). Logistic regression analysis showed blood glucose , white blood cell count , triglyceride level and NIHSS scores at admission were associated with sustained growth of Hs-CRP and the factors that they included Hs-CRP sustained growth , admission NIHSS scores, diabetes mellitus and pulmonary infection were regarded as independent risk factors. Conclusion The sustained growth of Hs-CRP in the acute phase of ischemic stroke is an independent predictor of progressive stroke.

Objective To explore the correlation between brain natriuretic peptide (BNP) and cerebral vasospasm (CVS) following subarachnoid hemorrhage (SAH) and hyponatremia. Methods Plasma BNP levels and serum natrium were assessed at 4 different time periods (1 to 3 days, 4 to 6 days, 7 to 9 days, and 10 to 12 days after SAH) in 30 patients with SAH. Correlation analysis was carried out between plasma BNP and blood sodium level. Plasma BNP levels were compared according to patients with or without CVS, and with or without symptom. The plasma BNP levels of 18 healthy controls were measured meanwhile.Results Plasma BNP levels in SAH patients were significantly elevated compared with control subjects ( P

Objective To investigate the positive rate of microembolic signal (MES) and the related factors,as well as the correlation between MES and outcomes in patients with cardiogenic cerebral embolism.Methods Patients with cardiogenic cerebral embolism were enrolled.The baseline data of the patients were collected and the MES monitor was conducted.The baseline data of the MES positive group and MES negative group were compared.Multivariatelogistic regression analysis was used to identify the related factors of the positive MES.The patients were followed up regularly.The outcomes of stroke at 6 months and recurrent stroke within 2 years in the MES positive group and MES negative group were compared.Results A total of 165 patients with cardiogenic cerebral embolism were enrolled,including positive MES in 68 patients (41.2％).There were significant difference in the levels of brain natriuretic peptide (BNP),cardiac troponin-I (cTn-I),and D-dimer between the MES positive group and negative group.Multivariate logistic regression analysis showed that the increased levels of baseline BNP (odds ratio [OR] 1.001,95％ confidence interval CI 1.001-1.002;P＜0.001),cTn-I (OR 36.975,95％ CI 1.516-902.0;P=0.027),and D-dimer (OR 1.001,95％ CI 1.000-1.001;P=0.017) were independently associated with the positive MES in cerebral embolism within 48 h after onset.There was no significant difference in the proportion of patients in good outcome (modified Rankin scale score 0-2) and poor outcome (modified Rankin scale score ＞2) after 6 months between the MES positive group and MES negative group.When the average follow-up time was 20.8 months (range,7-24 months),there were 23 patients (33.8％) and 19 (19.6％) had recurrence in the MES positive group and MES negative group,respectively.Kaplan-Meier analysis showed that the recurrence rate of stroke in the MES positive group was significantly higher than that in the MES negative group (log-rank test:P=0.031).COX regression analysis showed that the positive MES was still an independent risk factor for stroke recurrence after adjusting for other confounding factors (OR 0.328,95％ CI 0.142-0.761;P=0.009).Conclusions The positive MES was associated with the increased BNP,cTn-I,and D-dimer levds.The positive MES was not associated with clinical outcomes at 6 month after the onset,but it was associated with the recurrence of stroke within 2 years.

Objective To investigate the relationship between mean amplitude of glycemic excursions (MAGE) and cognitive impairment in elderly patients with type 2 diabetes.Methods Totally 80 patients (aged ＞ 60 years) with type 2 diabetic were selected.According to the Montreal Cognitive Assessment (MoCA) score,the patients were divided into two groups:cognitive impairment group (31cases) and non-cognitive impairment group (49 cases).Then all subjects were observed by continuous glucose monitoring for 3 days including the mean blood glucose (MBG) and its standard deviation (SD),MAGE and numbers of glycemic excursion (NGE).Results Compared with noncognitive impairment group,diabetic course,fasting blood glucose,2 h postprandial glycemia,glycosylated hemoglobin (HbA1c) and body mass index (BMI) were significantly different with the cognitive impairment group (P＜0.05).Furthermore,MAGE,MBG and SD were obviously higher than those in the non-cognitive impairment group [(5.89 ± 2.17) mmol/L vs.(3.15 ± 0.60) mmol/L,t＝8.37,P＝0.00; (11.85±3.45) mmol/L vs.(8.23±1.73)mmol/L,t＝6.23,P＝0.00; (3.16±1.29)mmol/L vs.(1.26±0.42)mmol/L,t＝9.57,P＝0.00].MoCA scores of patients with type 2 diabetes were negatively correlated with MAGE (r ＝-0.891,P ＜ 0.01).After multiple linear regression analysis,diabetic course,2 h postprandial glycemia,MAGE and SD remained independently associated with cognitive impairment in type 2 diabetic patients (R2 ＝0.835,P＜0.05)Conclusions MAGE during a daily period is associated with cognitive impairment independent of fasting blood glucose,postprandial glycemia and HbA1c.

ObjectiveToinvestigateRUNX3genepromoter methylationincolorectal carcinogenesis and its prognostic significance. MethodsThe protein expression of RUNX3 was detected by immunohistochemistry and the methylation status of the RUNX3 was determined by methylation-specific PCR (MSP) in colorectal normal mucosa and cancer tissue from 65 patients,and adenoma from 28 patients.5-year overall survival rate was analysed according to RUNX3 methylation status from cancer patients.Kruskal-Wallis rank sum test,x2 or Fisher's exact test,Log-rank test and multivariable Cox regression analysis were used for statistical analysis.ResultsThe protein expression of RUNX3 gene in adenoma and cancer was 85％ (24/28) and 52％ (34/65),significantly lower than that in normal mucosa 94％ (61/65)( x2 =4.328,P =0.037 ; x2 =16.675,P =0.000),the difference between adenoma and cancer tissue was no statistic significance (x2 =3.266,P =0.071 ).No case showed RUNX3 methylation in normal mucosa,methylation rates in adenoma and cancer tissue were 21％ (6/28) and 35％ (23/65),significantly higher than in normal mucosa (P =0.000 ),but there was no statistic significance between adenoma and cancer tissue (x2 =1.766,P =0.183).The protein expression rate with RUNX3 methylation was 67％ (4/6) in adenoma,unmethylation 91％ (20/22) (P =0.191 ).The protein expression rate with RUNX3 methylation was 26％ (6/23) in cancer,unmethylation was 88％ (28/32).The presence of RUNX3 methylation was related to loss of protein ( x2 =9.810,P =0.002 ).5-year total survival rate with methylation in cancer was significantly lower with unmethylation ( x2 =5.87,P =0.016 ).Multivariate analysis showed RUNX3 methylayion wasanindependentprognosticfactoramongthefactorsanalyzed(P=0.033 ).ConclusionsRUNX3 methylation is important genetic event in colorectal carcinogenesis,possibly related to protein downregulation,and an independent prognostic factor for colorectal carcinoma.