Objective To investigate the effect of aspirin on platelet biochemical index in patients with cardio-cerebrovascular diseases and the influence of Naoxintong(Brain-heart unobstruction)Capsule.Methods The blood samples of 215 patients taking aspirin 100 mg per day for 7 days were collected for determination of platelet aggregation(PAG),P-selection level and TXB2 level by using arachidonic acid(AA)and adenosine diphosphate(ADP).Then they were divided randomly into aspirin group 1(n=72),still taking aspirin 100mg per day,aspirin group 2(n=70),taking aspirin 300mg per day,and Naoxintong group(n=73),taking aspirin 100mg per day and Naoxintong Capsule.One month later,their PAG,P-selection and TXB2 levels were re-examined.Results The plasma P-selection and TXB2 were in a positive correlation with AA-and ADP-induced PAG(P

AIM: To examine the role of TLR-4 signaling pathway in laminar low shear stress-induced IL-8 gene expression in ECV304 cells. METHODS: RT-PCR and PCR were used to amplify a TLR4 mutant (lacking the 155 COOH terminal amino acids of the wild type TLR4 ) and-102-+61 bp 5′-flanking region of IL-8 gene (IL8 USCS)from endothelial cells. These two DNA fragments were cloned into pcDNA3 and pEGFP1, respectively, and the recombinant plasmid pcDNA3-mTLR4 and pEGFP1-IL8USCS were obtained. ECV304 cells were transfected with the pEGFP1-IL8USCS or co-transfected with pEGFP1-IL8USCS and pcDNA3-mTLR4 by using Dosper liposomal transfectional reagent and selected by G418, and then stimulated by 4 2 dyne/cm 2 shear stress for 3 hours. The green fluorescent protein expression was analyzed by Flow Cytometry. Immunoblotting of the cell lysates and NF-?B p65 immunocytofluorescent staining were used to determine I?B phosphorylation, degradation and NF-?B activation. RESULTS: Flow Cytometric analysis showed that when exposed to 4 2 dyne/cm 2 shear stress for 3 hours, there was a marked increase in the enhanced green fluorescent protein expression in pEGFP1-IL8USCS-transfected ECV304 cells. In contrast, there was almost no change in the green fluorescent protein expression in the cells co-transfected with pEGFP1-IL8USCS and pcDNA3-mTLR4 after the stimulation. Western blot analysis showed that a markedly increased p-I?B of cell lysates occurred at 10 min of exposure and the blot density almost dropped down to the baseline after 60 min of exposure. The density of I?B blot dropped down with increasing exposure time. NF-?B p65 immunocytofluorescent staining of ECV304 cells showed that when exposed to the same flow shear stress for 0 5,1 hours, the cell nuclei became staining, and after 1 5 or 2 hours, the staining was very strong. CONCLUSION: These results suggested that the inflammatory TLR-4/NF-?B signaling pathway may be involved in flow shear stress-induced IL-8 gene expression in human vascular endothelial cells.

Objective To investigate the expression and immune function of NOD2 signal in MyD88-/- mice. Methods MyD88-/- mice and wild-type C57 BL/6 mice were characterized by PCR. Mice model of pulmonary infection was constructed by tracheal instillation of BCG vaccine strain(attenuated strain of Mycobacterium). PBS tracheal instillation was used as negative control.Peripheral blood sample and lung tissue were collected aseptically 24 h after Mycobacterium challenge. Real-time PCR and Western blot were used to detect the expression of NOD2 gene and protein. IL-6 level in the peripheral blood was determined by enzymelinked immunosorbent assay. Results The expression of NOD2 protein in BCG infected mice was significantly higher than PBS negative control group. NOD2 protein expression in MyD88-/- mice was higher than in wild-type mice. BCG infection was associated with higher NOD2 protein expression than infection-free PBS control in both groups of animals. The IL-6 level in peripheral blood was significantly higher after BCG infection than PBS group in both MyD88-/- mice and wild type mice. Conclusions BCG can activate the NOD2 signaling pathway when MyD88-dependent pathway is deficient.

Objective: To evaluate the bond performance of primers, universal adhesives and self-adhesive resin cements containing methacryloyloxydecyl dihydrogen phosphate (MDP) to zirconia. Methods: Two hundred and ten yttria-stabilized tetragonal zirconia polycrystal (Y-TZP) plates were prepared and divided into seven groups according to each of six MDP-containing products and one MDP-free resin cement. Two primers, two universal adhesives and two self-adhesive resin cements were applied on the specimens after these specimens recived alumina air abrasion. The group of MDP-free resin cement was set as control. Each group contained 30 specimens. Y-TZP plates were bonded to resin composite to build resin bonding specimens, and half of these plates were stored in distilled water at 37 ℃ for 24 hours, the other half were stored for 12 months. Shear bond strength (SBS) test and fracture modes analysis were performed. Results: The SBS values of all the experimental groups were above 9.51 MPa, which increased 24-hour values compared to the (5.04±0.50) MPa of the control group. Group using one of the self-adhesive resin cement yielded the highest SBS, (11.06±0.84) MPa. SBS of all groups decreased significantly compared to 24-hour SBS after aging (P<0.05), however, the SBS values of MDP-containing products groups can still be maintained above 7.44 MPa. Two self-adhesive cement groups and one of the universal adhesive group maintained higher SBS values, and the values were statistical different from those from the two primer groups (P<0.05) . Conclusions Different MDP-containing products have different effects on the bond durability of zirconia.