Tie2 expressing monocytes (TEMs) only present in human circulating blood and tumor organs,and have an important role in tumor angiogenesis and progression.TEMs are effectively recruited to tumors by angiopoietin 2 (Ang2) and hypoxia and then differentiate into macrophages,which promote the angiogenesis in experimental tumor models by providing paracrine support to nascent blood vessels.Studies show that TEMs are up-regulated in hepatocellular carcinoma,colorectal cancer,breast cancer,malignant glioma and other human cancers,which suggests that TEMs are conducive to diagnosis and prognosis of tumors.With the further research,TEMs are applied to deliver drugs which can obtain significant anti-tumor responses and inhibit metastasis with the ability of tumor-homing.Meanwhile,TEMs may also be a potential target for the anticancer drugs.However,the present researches indicate that the effects of TEMs in tumor microvessel density,clinical stage and prognosis are still questionable.Current works that aim at describing and predicting the concrete function of TEMs have attracted significant attention from researchers.

Objective To study the risk factors for cerebral infarction resulting from transient ischemic attack (TIA).Methods The clinical data of 144 hospitalized patients with TIA were evaluated.Results 50 patients (34.7%) suffered from cerebral infarction(CI) following the onset of TIA in 144 patients.11 cases with CI resulted from vertebra-basilar artery system,37 cases resulted from internal carotid artery system. There was significantly different in two artery systems( P

Objective To investigate the hemodynamics of basilar artery and its blood-supply area in patients with vertigo by 3.0 T MR.Methods Fifty patients with vertigo were selected as case group and 50 healthy volunteers were selected as control group.All subjects in the case group and in control group were examined by brain magnetic resonance imaging (MRI),magnetic resonance angiography(MRA),fast cine phase contrast(Fast CINE PC) and flow-sensitive alternating inversion recovery sequence (FAIR).After image postprocessing,the velocity and the blood flow volume of basilar artery were measured and calculated.The FAIR value of bilateral occipital lobe,cerebellum,pons and medulla oblongata was measured.Statistical testing was performed.Results The average velocity,peak velocity and blood flow volume of the basilar artery in case group were significantly lower than those in the control group(the average velocity:(22.47 ±9.14) cm/s vs (31.41 ±5.97) cm/s,t =-5.79,P ＜ 0.0001 ; peak velocity:(31.60 ± 13.18) cm/s vs (44.00 ± 7.84) cm/s,t =-5.71,P ＜0.0001 ;blood flow volume:(91.14 ±43.92) ml/min vs (127.49 ± 28.33) ml/min,t =-4.92,P ＜0.0001).The perfusion of the posterior circulation in the case group was lower than that in the control group ((882.35 ±35.22) vs (906.34 ±36.82),t =-3.33,P =0.0012).The average velocity,peak velocity and blood flow volume of the basilar artery in case group whose brain parenchyma and blood vessels were normal were (26.31 ±5.16) cm/s,(36.33 ±6.60) cm/s,(95.38 ±28.16) ml/min respectively,which were all less than those in the control group (average velocity:t =-2.72,P =0.0086 ; peak velocity:t =-3.12,P =0.0027 ;blood flow volume:t =-3.53,P =0.0008).The perfusion of the basilar artery blood-supply area in case group whose brain parenchyma and blood vessels were normal was (904.00 ± 35.82),there was no difference when compared with the control group (906.00 ± 36.82,t =-0.20,P =0.8427).Conclusion Whether the brain parenchyma and blood vessels is normal or not,the velocity and blood flow volume of the basilar artery in patients with vertigo are decreased,but the perfusion of the basilar artery blood-supply area are different,it depends on whether there is ischemia.

Objective:To discuss about the reasonable use of antibiotics for infection of the CNS.Method:The classification and selection as well as the combinative use of antibiotics were reviewed individually. The role of blood-brain barrier,the use of drugs through myelin and their indications were also discussed.Results:Infections of the CNS have been controlled effectively through the use of antibiotic.Conclusion:Rationally using antibiotics in the treatment of infections of the CNS can relieve the economic burden of the patients.

Objective To establish a method of culturing mesenchymal stem cells from umbilical cord blood. Methods The mononuclear cell fraction of umbilical cord blood was isolated with the aid of a Ficoll/Hypaque gradient, then MSC were cultivated in serum containing medium or serum-free medium. The serum containing medium (SC) consisted of LG-DMEM supplemented with 10% fetal calf serum and 10-6M hydrocortisone, with a final cell concentration of 1?106 cells/ml. The serum-free medium (SF) was supplemented with 1?10 -6M hydrocortisone and 40ng/ml fibronectin with a final cell concentration of 1?106 cells/ml. After a week, 50% of the media were renewed. Before reaching the monolayer phase cells were trypsinised and re-cultured under similar conditions for the another 3 weeks. Cells were counted at the beginning and the end of the culturing period. Phenotypes were identifiad by PAS, NAE, ALP staining. Identification of surface markers and cell cycle analysis of MSCs were performed with flow cytometry. Results Adherent cells appeared 24h after plating of mononuclear cells. The cells formed adherent heterogeneous cell populations after 4-7 days in culture, and they consisted of round and spindle-like cells. In the primary passage of culture, the cells proliferated slowly and became confluent in 14-20 days. When subcultured, the heterogeneous cell populations became homogeneous assuming flat and fibroblast-like shape. Though colonies in the medium containing serum formed earlier than those in the serum-free medium, passage times and cell morphology were the same. The total cell numbers in SF group were lower than those of SC group. The percentage of cells at G0-G1 cell cycle was higher in SF group than that of SC group with significant difference. By flow cytometry analysis, cells of two groups were negative for CD34, CD13 and CD45, but strongly positive for specific surface markers such as CD166, CD29, CD105 and CD54. Conclusion Serum-free medium was superior to medium containing serum for culturing MSCs.

Objective To observe the effects of Xianfu Wenyang Tongluo Drink (XFWYTLD) on ANCA associated antigen in rats with thromboangiitis obliterans;To discuss its mechanism. Methods Totally 72 SPF male Wistar rats were randomized into sham-operation group, model group, Mailuoning Granule group and XFWYTLD low, medium, and high dose groups. Method of femoral artery injecting sodium laurate was used to duplicate models. From the second day after modeling, the rats in sham-operation group and model group were fed with distilled water, while other groups received gavage with relevant medicine. 15 days later, the activity of MPO in serum was detected through the method of ultraviolet spectrophotometry;the protein expressions of PR3 and LAMP-2 in femoral artery and the surrounding tissues were detected through the method of immunohistochemisty. Results Compared with sham-operation group, the activity of MPO in serum and the protein expressions of PR3 and LAMP-2 in rats of model group were significantly higher;Compared with model group, the activity of MPO in serum and the protein expressions of PR3 and LAMP-2 in rats of all medication administration groups decreased, among which the XFWYTLD medium dose group showed the most obvious decrease. Conclusion XFWYTLD may lower levels of ANCA associated antigen, and further inhibit humoral immune function.

Objective To evaluate the clinical and MRI features of choroid metastatic tumors.Methods Clinical and MRI findings in 4 cases with choroid lung carcinoma metastasis based on pathological results were retrospectively analyzed.Results Lung metastatic carcinomas were the most common primary cancers metastasizing to the choroid all the 4 cases had a decrease of visual acuity rapidly.MRI showed diffuse or local thickness of bulbar wall,and revealed T 1 isointense and T 2 iso-or hyperintense signals.A mass extruding into the bulb was seen in 1 patient.Conclusion MRI of choroid metastatic carcinoma has characteristic manifestations,and MRI is an effective and useful technique for the diagnosis of this lesion.

We aroused students' learning interest,adjusted teaching contents,played the appropriate role of teachers,optimized teaching means and carried out comprehensive evaluation based on special characteristics of the optional course environmental eugenics and the knowledge structure of students to improve the teaching quality and teaching effect as well as make this course contribute to comprehensive ability of medical students.

Objective To evaluate the effects of tofacitinib on liver function in rheumatoid arthritis (RA) patients.Methods Literature search was performed in databases including PubMed,Cochrane Library,China National Knowledge Infrastructure and Wanfang to identify randomized controlled trials about RA treated with tofacitinib.The retrieval time was up to August 2016.Meta-analysis was conducted by Revman 5.5 software.Results A total of 7 studies were included,involving 2 965 patients.The results of Meta-analysis revealed that the incidence of alanine transaminase (ALT)＞1 upper limit of normal (ULN) in patients receiving both 5 mg and 10 mg bid tofacitinib was significantly higher than placebo [5 mg bid tofacitinib:RR=1.48,95％CI (1.20,1.82),P=0.000 2;10 mg bid tofacitinib:RR=1.67,95％CI (1.37,2.05),P＜0.01];there was no significant difference in the incidence of ALT＞3 ULN [5 mg bid tofacitinib:RR=1.81,95％CI (0.57,5.79),P=0.32;10 mg bid tofacitinib:RR=1.36,95％CI (0.57,5.25),P=0.49];the incidence of aspartate transaminase (AST)＞1 ULN was significantly higher than placebo [5 mg bid tofacitinib:RR=1.59,95％CI (1.25,2.03),P=0.000 2;10 mg bid tofacitinib:RR=1.90,95％CI(1.50,2.40),P＜0.01],there was no significant difference in the incidence of AST＞3 ULN [5 mg bid tofacitinib:RR=1.17,95％CI (0.27,5.17),P=0.83;10 mg bid tofacitinib:RR=0.95,95％CI (0.26,3.44),P=0.94].Conclusion Tofacitinib slightly increases ALT and AST in patient with RA.Due to the limited sources and lack of domestic studies,more randomized controlled trials are still needed to verify the above conclusion.

Objective To observe the estrogenic activity of octylphenol(OP) in vitro and to conduct a preliminary study of its mechanism. Methods The estrogenic activity of OP was detected by cell proliferation test of MCF 7 cells in vitro and the mechanism was preliminarily studied by growth curve analysis, cell cycle analysis, tamoxifen(Tam) antagonistic test and apoptosis detection. Results OP was found to have estrogenic activity to stimulate the proliferation of MCF 7 cells. Cell cycle analysis revealed that the cell proliferation indexes of OP and 17? estradiol(E 2) were higher than those of alcohol. The estrogenic activities of OP and E 2 to stimulate the proliferation of MCR 7 could be antagonized by Tam. Both OP and E 2 could inhibit the cell apoptosis of MCF 7 cells. Conclusion OP possesses estrogenic activity to stimulate the proliferation of MCF 7 cells. The mechanism may be due to binding to the estrogen receptor, which may have effect on cell proliferation and apoptosis.