Objective To study the changes of negative symptoms in PCP-induced schizophrenia rat model.Methods Thirty newborn female SD rats randomly divided into control group,PCP-week 6 group and PCP-week 10 group( n=10 in each group).Perinatal rat treated with PCP ( 10 mg/kg) on postnatal days 7,9 and 11(10 mg/kg,ip),and sucorse intalce test(SIT),forced swimming test(FST) and resident-intruder test(RIT) were used to test the emotional and negative symptoms.Results In the SIT,there was no difference between control and PCP groups (con:(28.24 ±0.86) ml/kg; week 6:(26.57 ± 1.01 ) ml/kg; week 10:(27.98 ±0.99) ml/kg,F =12.35,P ＞ 0.05 ).In the FST,PCP model rats showed longer still time ( con:(39.32 ± 1.98 ) s ; week 6:(52.39 ± 1.66)s,week 10:(55.56 ± 1.49)s,F=3.99,P＜ 0.05 ).In the RIT,PCP models rats showed less explore time ( (40.31 ± 13.56)s vs (63.90 ± 13.12)s,(43.65 ±12.86 )s vs (65.18 ± 15.12)s,P ＜ 0.05 ) and more escape time ((19.33±2.26) s vs (9.26 ± 1.32) s,(17.79 ±2.99) s vs (9.38 ± 1.36) s,P＜ 0.05).Conclusion Perinatal PCP injection can induce the long-lasting negative-symptoms changes.

OBJECTIVETo study the chemical constituents from the whole plant of Aster poliothamnus.

METHODSeparating the chemical constituents by means of chromatography and identifying ther structures on basis of chemical and spectral tecnology.

RESULT AND CONCLUSIONA new triterpene saponin was isolated and identified.

Aster Plant ; chemistry ; Molecular Structure ; Plant Roots ; chemistry ; Plants, Medicinal ; chemistry ; Saponins ; chemistry ; isolation & purification

Objective To discuss the relationships between regular pattern changes of plasma fibrinogen (Fib),D-dimer and fibrinogen degradation products (FDP) levels and the recent dissolution of thrombus in patients with pulmonary embolism (PE) in 14 days after treatment.Methods A prospective study was conducted.PE patients admitted to Departments of Respiratory Disease in 4 hospitals from January 2015 to March 2016 were enrolled and all of them were treated with thrombolysis and/or anticoagulation after admission.The computed tomographic pulmonary angiography (CTPA) was examined pre-treatment and 14 days post-treatment in PE patients.The pulmonary artery obstruction index (PAOI) was assessed according to the Mastora scoring method to estimate the thrombus load.The plasma Fib,D-dimer and FDP levels were measured before and on 1,2,3,5,7 and 14 days after treatment,and the relationships between the change regularities of these parameters and PAOI were also analyzed.Results A total of 42 PE patients were enrolled.The curve change of coagulation-fibrinolytic system parameters in 14 days after treatment showed that the Fib level was raised to its peak on the 3rd day after treatment and then decreased (g/L:4.24 ± 1.45 vs.3.83 ± 1.56),representing that its curve change was in accordance with the quadratic model (P =0.095).After treatment,the D-dimer and FDP levels were kept declining,they were reached the valley on 14th day [D-dimer (mg/L):1.58 ± 1.38vs.8.84 ± 6.35,FDP (mg/L):4.23 ± 3.63 vs.23.41 ± 16.54],and their curve changes were in accordance with the cubic model (F was 32.190 and 34.326,respectively both P =0.000).The PAOI variation before and 14 days after treatment [(18.77 ± 14.22)％] was not correlated with Fib variation [(1.20 ± 0.93) g/L,r =-0.194,P =0.219],but was positively correlated with D-dimer variation [(7.29 ± 7.10) mg/L] and FDP variation [(19.29 ± 18.67) mg/L,r was 0.556 and 0.460,respectively;P was 0.020 and 0.002,respectively].Conclusions The D-dimer and FDP levels are kept falling in PE patients after treatment,suggesting that the pulmonary artery embolus is being dissolved.

Objective: To summarize the physical and psychosocial problems of burned children aged 0 to 5 years so as to explore their demands of care after discharge. Methods: Databases including Embase, PubMed, PsycARTICLES, and CINAHL Complete were searched using key words " child^*, infant^*, toddler, preschool, kid, baby, junior, burn, scald, empyrosis, nursing, care" , and Chinese Journals Full-text Database, China Biology Medicine disc, and VIP Database were searched using key words in Chinese version "小儿,儿童,幼儿,学龄前,婴儿,新生儿,烧伤,烫伤,照护" to obtain the articles about physical and psychosocial health-related problems of burned children aged 0 to 5 years after discharge from the establishment of each database to February 2018. After screening of extracted articles, the physical and psychosocial problems of burned children aged 0 to 5 years after discharge and psychosocial problems of their caregivers were summarized. Results: (1) A total of 3 880 articles were obtained, after screening layer by layer, 9 English articles were finally included, including 4 prospective observational studies, 3 cross-sectional studies, 1 cohort study, and 1 case-control study, with 1 high-quality literature and 8 medium-quality literatures. (2) After discharge, burned children aged 0 to 5 years mainly had physical problems such as pain, itching, fine motor developmental disorders, and gross motor developmental disorders. They had psychosocial problems such as negative emotional behavior, delayed language development, affected game interaction, post-traumatic stress disorder (PTSD), and disrupted family life. The caregivers had psychosocial problems such as worrying about the appearance and the future of children, dissatisfaction with the current status of children, anxiety and depression, pain, and PTSD. The influencing factors of the above health problems were post-discharge time, post-injury time, total burn area, and burn site, etc. There were 12 kinds of measuring tools used in this group to evaluate the health problems of burned children and psychosocial problems of their caregivers. Only the burn outcomes questionnaire for children aged 0 to 5 years was developed for burned children aged 0 to 5 years. Conclusions After discharge, burned children aged 0 to 5 years still face many physical and psychosocial problems, which are influenced by factors such as post-discharge time, post-injury time, total burn area and burn site, which suggests that there are certain care needs for them. Appropriate tools for evaluating the health problems of burned children aged 0 to 5 years should be developed.

Objective: To observe the effect of ulinastatin combined with glutamine on early hemodynamics in patients with severe burns. Methods: Thirty-two patients with severe burns who met the inclusion criteria and hospitalized in the Affiliated Huaihai Hospital of Xuzhou Medical University from January 2016 to December 2018 were selected for conducting a prospective randomized controlled trial. According to the random number table, the patients were divided into conventional treatment group (4 males and 4 females), ulinastatin group (5 males and 3 females), glutamine group (5 males and 3 females), and ulinastatin+ glutamine group (4 males and 4 females), with ages of (36±8), (34±8), (35±9), and (38±13) years in turn. From post injury day 2, patients in the 4 groups were given nutritional support of equal nitrogen and equal calories, of which protein was 2.0 g/kg daily. In addition, patients in the ulinastatin group received intravenous injection of 100 kU ulinastatin every 8 hours for 7 consecutive days; 0.3 g/kg of protein given to patients in the glutamine group was provided by alanine glutamine for 7 consecutive days; patients in the ulinastatin+ glutamine group received corresponding treatments of both ulinastatin group and glutamine group. With the help of pulse contour cardiac output (PiCCO) monitoring technology, the cardiac index, stroke volume index (SVI), global end-diastolic volume index (GEDI), systemic vascular resistance index (SVRI), extravascular lung water index (EVLWI), pulmonary vascular permeability index (PVPI) of patients in each group were measured on treatment day (TD) 1, 3, and 7. Data were processed with Fisher′s exact probability method, one-way analysis of variance, analysis of variance for repeated measurement, and Bonferroni method. Results: The cardiac index was low and the SVI value was lower than the normal value on TD 1 in patients of the 4 groups, without statistically significant differences between any two groups (P>0.05), and then they were all gradually increased. On TD 3 and 7, compared with those of the conventional treatment group, the cardiac index and SVI of patients in the other three groups were all increased, and the cardiac index and SVI of patients in the ulinastatin+ glutamine group were significantly increased (P<0.05 or P<0.01). On TD 1, the GEDI of patients in the conventional treatment group, ulinastatin group, glutamine group, and ulinastatin+ glutamine group were at normal low levels, which were (659±58), (661±79), (659±88), and (653±71) mL/m² respectively, without statistically significant differences between any two groups (P>0.05), and then they all gradually increased. On TD 3 and 7, compared with (684±82) and (742±46) mL/m² of the conventional treatment group, the GEDI of patients in the ulinastatin group, glutamine group, and ulinastatin+ glutamine group were all elevated, which were (732±53) and (777±33), (725±58) and (783±49), (813±65) and (849±27) mL/m² respectively, and the GEDI of patients in the ulinastatin+ glutamine group was significantly increased (P<0.05). The SVRI of patients in the four groups were all at high levels on TD 1, without statistically significant differences between any two groups (P>0.05), and then they all gradually decreased. On TD 3 and 7, compared with those of the conventional treatment group, the SVRI of patients in the other three groups were all increased, and the SVRI in the ulinastatin+ glutamine group was significantly increased (P<0.05). On TD 1, the EVLWI of patients in the conventional treatment group, ulinastatin group, glutamine group, and ulinastatin+ glutamine group were all in the normal range, which were (6.6±0.6), (6.3±0.4), (6.5±0.4), and (6.6±0.6) mL/kg respectively, without statistically significant differences between any two groups (P>0.05), and then they all showed the increasing trend. On TD 3 and 7, compared with (7.1±0.9) and (7.9±0.5) mL/kg of the conventional treatment group, the EVLWI of patients in the ulinastatin group, glutamine group, and ulinastatin+ glutamine group were all decreased, which were (6.2±0.6) and (7.1±0.4), (6.3±1.0) and (7.2±0.9), (5.8±0.7) and (6.7±0.6) mL/kg respectively, and the EVLWI of patients in the ulinastatin+ glutamine group was significantly decreased (P<0.05). On TD 1, the PVPI of patients in the four groups were all in the normal range, without statistically significant differences between any two groups (P>0.05), and then they all gradually decreased. On TD 3 and 7, compared with those of the conventional treatment group, the PVPI of patients in the other three groups were all decreased, and the PVPI in the ulinastatin+ glutamine group was significantly decreased (P<0.05). Conclusions Ulinastatin combined with glutamine can increase the cardiac index, SVI, GEDI, and SVRI and reduce the EVLWI and PVPI in treating patients with severe burns, thereby increasing early cardiac output after injury, promoting tissue and organ perfusion, and reducing pulmonary edema, resulting in significant improvement in early hemodynamics of patients with severe burns.

Objective To understand the prevalence of chronic kidney disease (CKD) in the elderly in xuzhou area and the main related factors, and to provide a theoretical basis for the prevention and treatment of CKD. Methods Xuzhou gulou, mountain springs, deals for the city select venture, peixian county rural countryside, deals with the community (village) as unit, adopt the method of stratified cluster sampling, a random sample of urban and rural community people aged 60 to 79, and residents living longer than six months as investigation object, obtain 482 valid data, chronic kidney disease (CKD) sampling investigation, All subjects underwent conventional questionnaires, including gender, age, occupation, chronic disease history, family history, etc. Laboratory tests included, UA, SCr, FBG, 2H PBG, TG and TC levels. EGFR was calculated based on SCr, and logistic regression was used to analyze the independent risk factors for CKD in the elderly in community. Results Among 482 elderly patients, 259 were males and 223 were females, with an average age of (45.73±6.14) years. 63 patients with CKD were diagnosed, with a prevalence rate of 13.07%. The prevalence of chronic diseases was 20.33%(98/482) for hypertension, 15.15%(73/482) for hyperuricemia, 11.00%(53/482) for overweight or obesity, 8.71%(42/482) for hyperlipidemia and 6.430%(31/482) for diabetes. The prevalence of CKD in 70-79 year olds was significantly higher than that in 60-69 year olds (χ²=6.621 , P<0.05). There were statistically significant differences in age, hypertension, diabetes and hyperuricemia between 2 groups (P<0.05). Logistic regression analysis showed that advanced age, hypertension, diabetes and hyperuricemia were independent risk factors for CKD in elderly population in Xuzhou area (P<0.05). Conclusion The prevalence of CKD in the elderly in Xuzhou community is high, and the main risk factors are advanced age, diabetes, hypertension and hyperuricemia. It is necessary to actively carry out early screening of CKD and take effective preventive measures to effectively delay the development of CKD and prevent the occurrence of end-stage renal disease.

Objective: To investigate the molecular and signal pathway mechanism of Interleukin-27 affecting the anti-tumor effect of NK92 cells. Methods: NK92 cells were cultured with different concentrations of IL-27 (10, 20, 30 and 60 ng/ml) for 24 hours. The cytotoxicity of NK92 cells to target cells was detected by LDH assay. The expressions of NKG2D, NKp30 and NKp46 on the surface of NK92 cells and the secretion of perforin and granzyme B were detected by Flow cytometry. The expression and phosphorylation level of STATs protein was detected by WB. The DU145 cell transplanted tumor model of prostatic carcinoma in NOD-PrkdcscidIl2rgem1/Smoc mice was established and treated with the combination of NK92 cells and IL-27 to evaluate their anti-tumor efficacy. Results: IL-27 at concentrations of 10, 20 and 30 ng/ml could significantly increase the cytotoxicity of NK92 cells to target cells, and 30 ng/ml exerted the best effect (P<0.05 or P<0.01). 30 ng/ml IL-27 could significantly promote the expressions of NKG2D, NKp30 and NKp46 on surface of NK92 cells, as well as elevate the secretion of perforin (all P<0.05), but didn’t affect the secretion of granzyme B (P>0.05); moreover, it also up-regulated the phosphorylation of STAT1, STAT3 and STAT5 protein (all P<0.01). The combined treatment of IL-27 and NK92 cells obviously extended the survival time of tumor-bearing mice (P<0.05). Conclusions: IL-27 can promote the cytotoxicity of NK92 cells against solid tumor cells and blood tumor cells by promoting expressions of NKG2D, NKp30 and NKp46 on the surface of NK92 cells and the secretion of perforin, which might be related with the phosphorylation of STAT1, STAT3 and STAT5 in JAKSTAT pathway.