A high-performance liquid chromatographic procedure for the simultaneous determination of vitamin B1, B2 and niacin in fortified mixed protein was developed. The sample was heated on water bath by adding 0.1N HC1 at 100℃ for 30 min. Afterward, the vitamins in the filtrate of the sample were separated on a column of u-bondapak C18 with methanol/water containing 0.005M pic B7 ion pair reagents (40:60 V/V) as mobile phase, and then the eluate was measured at 254nm. The detection limits for vitamin B1, B2 and niacin were approximately 15 ng, 5ng and 6ng respectively. The average recoveries of vitamin B1, B2 and Niacin were 92.21,90.25 and 92.33% respectively. The coefficients of variation of these vitamins were all under 6%. The method is simple, rapid and accurate, so it could also be applied to the analysis of other samples such as fortified foods and multivitamin preprati-ons etc.

The contents of the individual fractions of glucose oligosaccharides were determined simultaneously by means of reversed-phase HPLC. The sample was dissolved in water and filtered and the filtrate was used directly for the analysis. The sugar was separated on a column of u-Bondapak C18 using water as the mobile phase and determined refractometrically.The method was applicable to the analysis of glucose oligosaccharides from monomeric glucose through polymeric maltooctose. Quantitative results were obtained for glucose, maltose, maltotriose, maltotetrose, maltopentose and maltohexose in the samples prepared in our laboratory. Recovery tests revealed this method reliable.

The effects of grape seed oil on serum total cholesterol (TC) and high density lipoprotein cholesterol (HDL-C) levels were investigated in experimental hypercholesterolemic rats. The grape seed oil containing about 74% linoleic acid, multivitamins and trace elements. Each animal was given 4 ml/kg BW grape seed oil mixed in high fat ration for 40 days. The results in two experiments showed that TC concentration in the serum of rats fed grape seed oil was markedly lowered as compared to control, but serum HDL-C was less lowered. Furthermore, the ratio of HDL-C to TC in the experimental group was also significantly higher than that of control.