With the development of the time,its original management system problem revealed. Then how to reform in order to play its due role? The author pondered on the college student associations'development prospects and ideas by analysing its status and the plight of its development.

10 000 u,5 000~10 000u and 10 000 u group,and the gene expression was slightly increased in molecular weight 5 000～10 000 u group,but no significant difference of gene expression and protein secretion in 10 000 u uremic toxin,through promoting the gene expression and protein excretion of TGF-?_1 of renal tubular epithelial cells in patients with chronic renal failure.

Objective To explore the application value of mammary gland remodeling to one-stage repair local defects after partial excision.Methods The clinical data of 62 breast patients undergoing mammary gland remodeling to one-stage repair local defect after partial excision between January 2011 and December 2014 were reviewed.Results All patients recovery,the average operative time was 60 min,the average hospital stay was 7 days,the average cost was 5 216 yuan,1 case with postoperative concurrent incision infection,6 cases with postoperative hematoma.The cosmetic results were excellent or good rate was 87.1％ (54/62),and fair rate was 12.9％ (8/62).The breasts showed natural and symmetrical appearance during a follow-up of 12-36 months.Conclusions The application of mammary gland remodeling to one-stage repair local defect after partial excision is a safe and economical method.

Objective To compare elasticity imaging and conventional ultrasound when applied to the diagnosis of benign and malignant breast tumors.Methods Totally 124 breast tumor patients (140 lesions) from some hospital were analyzed retrospectively,who were divided into a control group and an observation group according to ultrasound examination ways.The control group involved in 60 patients with 66 lesions undergoing conventional ultrasound examination,and the observation group had 64 patients with 74 lesions undergoing elasticity imaging.Pathological findings were considered as gold standard,and the two groups were compared on accuracy,specificity and sensitivity.Results Pathological detection found the accuracy,specificity and sensitivity of the malignant tumor in the control group were 85.00％,84.78％ and 84.85％ respectively,and the ones of the malignant tumor in the observation group were 95.83％,96.00％ and 95.95％ respectively.The two groups had significant differences between the accuracies,specificities and sensitivities (P＜0.05).Conclusion Elasticity imaging describes the size,shape,number,margin and etc of the breast tumor,and thus can enhance the accuracy,specificity and sensitivity for diagnosing breast tumors.

Objective] The pathogenesis of HuangQin Tang is discussed little in six meridians syndrome differentiation, and which meridian syndrome does it belong to isn’t clear. So this article tries to discuss the belonging and pathogenesis of it. And the key point of using it in clinic will also be involved.[Method] Combining the observation of efficacy of XiaoChaiHu Tang and HuangQin Tang and the elaboration articles are related to that two prescriptions to analyze the difference of that two prescriptions in pathogenesis.[Result]It’s found that HuangQin Tang and XiaoChaiHu Tang all belong to ShaoYin in six meridian syndrome.But their pathogenesis is different. HuangQin Tang should be used in heat syndrome of ShaoYang disease which caused by heat pathogen of ShaoYang meridian and diarrhea will appear because belly is shocked. However, XiaoChaiHu Tang is used to drive cold pathogen of ShaoYang meridian. [Conclusion]Both of HuangQin Tang and XiaoChaiHu Tang belong to ShaoYang Meridian, but it’s known that HuangQin Tang is used less. And less discussion of HuangQin Tang should be blamed. The more we know on pathogenesis of HuangQin Tang, the better we use it.

Objective To investigate the effect of diosmin on the pain and wound healing after modified anal fissure resection .Methods 98 patients from January 2015 to July 2016 in our hospital who were admitted to meet the inclusion criteria and improved anal fissure resection patients were selected and randomly divided into control group and study group, 49 cases in each group.And the control group was given routine postoperative treatment, the study group was treated with diosmin tablets on routine postoperative therapy , two groups were treated for seven days for a course of treatment, the wound pain, wound width and wound healing were observed and recorded before and after treatment, blood samples were taken to determine changes in serum inflammatory factors, the clinical efficacy and complications were compared.Results After treatment, the two groups of wound pain, the width of the wound and wound healing rate were decreased, the serum TNF-α, IFN-γ, IL-6 levels were decreased, the difference was statistically significant (P<0.05), compared with the control group, the degree of pain, the width of the wound and wound healing rate were lower, the serum TNF-α, IFN-γ, IL-6 content were lower, the average healing time and granulation growth time and remove decay time were shorter, the difference was statistically significant (P<0.05).The effective rate of the study group was 91.83%, higher than the control group 75.51%, the difference was statistically significant (P<0.05).All patients were followed up, no case falls off phenomenon, there was no significant difference in the incidence of adverse reactions between the two groups.Conclusion Diosmin tablets on the improvement of anal fissure after resection of wound healing has a role in promoting inflammation, reduce the severity of pain conditions.

Background and purpose:Uterine body invasion of carcinoma of the uterine cervix is not in the International Federation of Gynecology and Obstetrics(FIGO)staging system.Some reports suggested that uterine body invasion of carcinoma of the uterine cervix was an important prognostic factor.The aim of this study was to investigate the clinical characteristics and prognosis of the uterine cervix carcinoma with uterine body invasion.Methods:The clinicopathologic records of 406 patients with carcinoma of the uterine cervix of stage Ⅰb~Ⅱa who underwent radical hysterectomy and pelvic lymphadenectomy were retrospectively analyzed,the number of uterine body invasion was 69 in all cases.On the contrary,the negative number was 337.We studied the general condition(age of onset)and tumor conditions,including clinical stage,tumor diameter,pathological type,pathology differentiated degree,positive pelvic lymph nodes,deep stromal invasion,and lymph vascular space involvement.Results:In univariate survival analysis,the incidence of stage Ⅱa,non-squamous cell cancer(non-SqCC),tumor size≥4 cm,deep stromal invasion and positive pelvic lymph nodes were signifi cantly higher in carcinoma of the uterine cervix with uterine body invasion than those in control group(P

Objective:To study the effect of interleukin-18 on transdifferentiation in renal tubular epithelial cells(TECs).Methods:Human proximal tubular epithelial cell line(HK-2) was cultured in vitro.TECs were exposed to different concentrations(0,0.1,1,10 and 100 ng/ml) of IL-18 for 24,48 and 72 hours.At the end of each incubation,the expressions of the ?-SMA and TGF-?_1 mRNA were assessed by RT-PCR,the rate of ?-SMA expressing TECs was assessed by immunocytochemistry,and the expression of the ?-SMA protein was assessed by Western blotting.Results:(1)The expressions of ?-SMA and TGF-?_1 mRNA were increased significantly by a dose- and time-dependent manner when TECs were exposed to IL-18(P

Objective To investigate the effect of cyclosporine A (CsA) on autophagylysosomal pathway in tubular epithelial cells.Methods Human renal tubular epithelial cell line (HK-2 cell) was treated with different concentrations (3,5 and 10 μmol/L) of CsA for 24 h.Then the viability and apoptosis of cells were measured by MTT assay or AnnexinV-PI staining followed by flow cytometry analysis,respectively.Autophagy-related protein LC3-Ⅱ and p62 were detected by immunofluorescence assay.Autophagic flux was analyzed in HK-2 cells transfected with a tandem mRFP-GFP fluorescent-tagged LC3 (ffLC3) plasmid by laser confocal microscope.The lysosomal degradation was evaluated by DQ-ovalbumin staining followed by flow cytometry analysis.Results The viability of HK-2 cells was significantly decreased with CsA stimulation when compared with control group (P ＜ 0.01),but the number of apoptotic cells was markedly increased by CsA treatment (P ＜ 0.05).Compared with the control group,different doses of CsA dramatically increased the expressions of LC3-Ⅱ (P ＜ 0.01) and p62 (P ＜ 0.05) in HK-2 cells.Moreover,HK-2 cells treated with CsA displayed a significant increase in autophagosomes but a marked decrease in autolysosomes.In HK-2 cells,exposured to CsA caused a decrease in lysosomal degradation by DQ-ovalbumin staining when compared with control group (P ＜ 0.01).Conclusion Blockade of autophagy via disrupting lysosome degradation may represent a novel mechanism of CsA-induced tubular epithelial cells injury.

Aim To investigate the effects of Panax Notoginsenosides(PNS) on the gene expression and protein excretion of TGF-?_1 and CTGF of human renal tubular epithelial cell induced by uremic serum in vitro.Methods Forty sera from CRF patients and twenty sera from healthy volunteers were collected,gently mixed,inactived and separated respectively in steriled condition.HK-2 Cells were cultured in RPMI-1640 medium with 10% newborn calves serum and subcultured routinely.They were differentiated by phase contrast microscope and scanning electron microscope detection and cytokeratin18(CK-18) immunohistochemistry method.The protein levels of TGF-?_1 were examined by enzyme-linked immunoadsordent assay(ELISA).The protein levels of CTGF were examined by Western Bloting assay.The gene expression of TGF-?_1 and CTGF was detected by Semi-quantitative reverse trainscriptase polymerase chain reaction(RT-PCR).Results TGF-?_1 and CTGF gene expression and protein level were increased in 10% uremic serum groups compared with that of normal control group(P