Objective The quantitation measurement of microalbuminuria was adopted to verify the feasibility of urinary microalbumin and ratio of mieroalbumin to creatinine (M/C) detection with dry hemieal method. Methods Totally 80 urine samples from the patients with clinically diagnosed dia-betes were tested by the immune quantitation method (immunoturhidimetric assay) and dry heroical method (HT2000 urine analyzer and test paper, Guilin Hnatong Company) simultaneously. Sarcosine oxidase method was applied to measuring creatinine level. Ten cases of outliers were removed. With the quantitation result of M/C as the reference standard, immune quatilyzation of the M/C as refer-ence, we compared and analyzed the results of the immune quantilization of urine microalbumin, those of the dry chemistry method and of the dry chemistry system detecting M/C. Results There was sig-nificant differences in test results of urinary microalbumin with the dry chemistry method and the im-mune quantitation method (P<0.01) The sensitivity, accuracy and Youden index of the microalbu-minuria testing had been observed to decrease in order in immune the dry chemistry method, the im-mune quantitation method and M/C quantitation detection method. Conclusion The sensitivity of semi-quantitation dry chemical method is satisfactory in detecting microalbumin, which may be used as a means of microalbumin screening. M/C detection with the dry chemical method isn't suitable for screening microalbumin in instant urine samples.

In recent years,experts of international nephrology,emergency medicine and other disciplines tend to substitute acute kidney injury for acute renal failure.There is a new point of view about the definition,diagnostic criteria,staging,management and prognosis of acute kidney injury.With diagnostic criteria constantly updated,it comes up to an expectation of united criteria as well as reaching a consensus.Continuously looking for more suitable for both time window of clinical diagnosis and clinical observation indicators,and clinically making out much more scientific and reasonable standard of diagnosis and staging,which contribute to early detection and early intervention as a result of eventually mortality rate reduction.This paper gives an interpretation for the procedure of evolution about diagnostic criteria on acute kidney injury in children according to the related literatures updated in recent years.

Acute kidney injury(AKI) is a common critical syndrome involving multidisciplinary among pediatric clinical disorders.Early identification and early intervention in severe cases with kidney damage,which can reduce mortality and improve prognosis.Renal replacement therapy(RRT) is an important means in the treatment of AKI,but there is still lacking of ideal and general modality and doses of RRT at present.Understanding the risks and benefits of each modality of RRT in the treatment of AKI is helpful to evaluate RRT's influence on the prognosis of children in AKI.Therefore the selection of modality of RRT should clinically be based on hospital conditions and specific patient characteristics,in order to assure that the choice of modality of RRT is the most suitable for the individual.

Objective: To assess the diagnosis value of applying integrated visual and auditory continuous performance task(IVA-CPT) and EEG biofeedback on children with attention-deficit- hyperactivity disorder(ADHD), and provide information for assisting diagnosis of ADHD. Methods: 113 children with ADHD were tested with IVA-CPT and EEG biofeedback. The sensitivity, specificity, misdiagnosis rate and missed diagnosis rate of these two methods were assessed according to the criteria of DSM-Ⅳ. Results: The sensitivity of IVA-CPT for the diagnosis of children with ADHD was 92.54, the specificity was 76.09%, misdiagnosis rate was 23.911% and missed diagnosis rate was 7.46%. And the sensitivity of EEG biofeedback for the diagnosis of children with ADHD was 83.58%, the specificity was 82.61%, misdiagnosis rate was 17.38 and missed diagnosis rate was 16.42%. Conclusion: Relatively, for diagnosis of children with ADHD, sensitivity is higher and missed diagnosis rate is lower, specificity of EEG biofeedback is higher and misdiagnosis rate is lower .

Objective To evaluate the efficacy of combination treatment of montelukast sodium (singulair TM ) plus mycobacterium phlei F.U.36 (wtilin"s") on young children with asthma.Methods 96 young children with asthma were randomly assigned into GroupⅠ:montelukast sodium (orally 2 5mg,q.n) plus mycobacterium phlei F.U.36 intramuscular injection for a total of 6 ampules,Group Ⅱ only montelukast sodium orally 2 5mg nightly and placebo Group.Participants were followed up for 12 weeks.Resuts Combined treatment of montelukast sodium and mycobacterium phlei F.U.36 was more effective to improve asthma in young children than the treatment with only monstelukast sodium.Conclusion The combination therapy of monkelukast and mycobacterium phlei F.U.36 is a highly effective method for preventing asthma attacks or controlling asthma.

Objective To evaluate the clinical efficacy of expulsive therapy using alpha 1-selective adrenoblocker and prostaglandin synthetase inhibitor for distal ureteral stones.Methods 94 patients with distal ureteral stone were randomly divided into study group and control group.In study group,47 cases received tamsulosin 0.2mg daily and dielofenac rectal suppositories 50mg 2 times daily,while watchful waiting without tamsulosin and diclofenac suppository in 47 cases of control group.Observation lasted 2 weeks and also stone expelled as end point.Results No severe adverse reactions related to the drugs were noted and no patients withdrew from the study.The stone-free rate was 91.5%(43/47) of the study group and 25.5% (12/47) of the control group (P＜0.01).A mean stone expelling date of the control group was(8.9±4.3)d and that of the study group was(6.4±3.7)d(P＜0.01).Rates of renoureteral colic recurred in the study group and the control group were 4.3%(2/47) and 48.9%(23/47),respectively (P＜0.01).Conclusion Treatment of distal ureteral stones wlth tamsulosin and dielofenac suppository can increase the stone-free rate,shorten mean stone expelling date,decrease rate of renottreteral colic recurred.

SuperparamagneticSiO2@Fe3O4microspherewithcore-shellstructurewaspreparedbythe method of hydro-thermal and St?ber method, and modified by (3-aminopropyl) triethoxysilane ( APTES) . The obtained amino-Fe3 O4@SiO2 microsphere with controlled surface charge properties was characterized by SEM and TEM, and the surface electric property of amino-microsphere was investigated by Zeta potential measurement. The microsphere was used to extract DNA from human blood, and the solid phase extraction method by microspheres was developed. The mechanism of action between Aminated-Fe3 O4@SiO2 microsphere and DNA was explored, and the gel electrophoresis and PCR test were done on the extraction product. The result showed that genomic DNA with high purity was successfully extracted from whole blood by using amino-Fe3 O4@SiO2 microsphere, with the extraction rate about 70%, the saturated adsorption capacity for DNA was about 40 ng/μg, and the elution could be directly used for further bio-analysis.

The TLD(thermoluminescent dosimetry)must be calibrated before used in individual dosimetry.In this paper,some related issues that affect the accuracy of calibration,including principle and performance of the instruments,conditions of annealing and measuring,method of curve fitting are discussed and an appropriate calibration method is proposed.The results show that the method we proposed is valid and efficient,which ensures the accuracy of individual dosimetry.

Objective: To develop an HPLC-ELSD method for the simultaneous determination of six saponins constituents including notoginsenoside R1, ginsenoside Rg1, ginsenoside Re, ginsenoside Rb1, ginsenoside Rc and ginsenoside Rd in Xinling pills.Methods: HPLC-ELSD was used, and the chromatographic separation was performed on an Agilent Eclipse XBD-C18 column (150 mm×4.6 mm,5 μm) with acetonitrile-water as the mobile phase with gradient elution at the flow rate of 1.0 ml·min-1, the column temperature was maintained at 20℃, the drift tube temperature was 60℃, and the gas pressure was 4.00 bar.Results: Notoginsenoside R1, ginsenoside Rg1, ginsenoside Re, ginsenoside Rb1, ginsenoside Rc and ginsenoside Rd was linear within the range of 0.30-6.00μg(r=0.999 5), 1.14-22.80μg (r=0.999 6), 0.17-3.40 μg (r=0.999 7), 0.81-16.20 μg (r=0.999 7), 0.08-1.60 μg (r=0.999 8) and 0.07-1.40 μg (r=0.999 8), respectively.The average recovery was 98.23%, 97.98%, 99.14%, 99.15%, 98.72% and 98.37%, and the RSDs were 1.56%, 1.31%, 1.16%, 1.07%, 0.73% and 0.92%(n=6), respectively.Conclusion: The method is convenient, accurate and reproducible in the quality control of saponins components in Xinling pills

Objective To investigate the relationship between the clinical features of patients with different cancer and their clin‐icalstage,lymphnodemetastasissituation.Methods 135cancerpatientsdiagnosedandtreatedinthehospitalfromJanuary2010to December 2014 were enrolled in the study ,in addition to that ,57 people who underwent healthy examination in the hospital and proved to be healthy were also recruited as control group .Prothrombin time(PT) ,thrombin clotting time(TT) ,activated partial thromboplastin time(APTT) ,D‐dimer ,fibrinogen(FIB) were tested for the people mentioned above .Results The level of routine coagulation indicators were statistically significant different between people with different types of cancers and control group(P<0 .05) .Compared with the control group ,PT ,APTT of the cancer patients significantly shortened ,FIB ,D‐dimer levels were signifi‐cantly increased(P<0 .05) .PT ,APTT was prolonged in lung cancer ,esophageal cancer ,breast cancer ,stomach cancer compared with lung cancer ,FIB ,D‐dimer decreased compared with other malignancies(P<0 .05) .PT ,APTT was decreased and D‐dimer ,FIB was significantly increased in cancer(lung ,esophagus ,breast ,stomach) with Ⅲ‐Ⅳ stage or lymph node metastasis than Ⅰ‐Ⅱstage or non‐lymph node metastasis ,the difference was statistically significant(P<0 .05) .Conclusion The malignant tumors were with hypercoagulable state ,there are differences in coagulation in different clinical stages ,lymph node metastasis .