1.Transvaginal ultrasound monitoring of gestational sac size and drug abortion
Chinese Journal of Biochemical Pharmaceutics 2017;37(5):397-398,402
Objective To study the effect of transvaginal ultrasound on the size of gestational sac and drug abortion, and to explore the relationship between the effect of drug abortion and the gestational sac size.MethodsIn our hospital in July 2016 were treated 50 cases of drug abortion of pregnant women as the research object in the process in February 2015, a retrospective analysis of 50 cases of pregnant women, patients were divided into gestational sac and greater than 10 and less than 10mm to 25mm 25mm three.Transvaginal ultrasound was performed before taking the medicine.After 7 days, the patients were examined by transvaginal ultrasound.The results showed that 7 patients had abortion failure, and the other 5 patients had incomplete abortion.ResultsTransvaginal ultrasound monitoring results can be obtained, the gestational sac size of less than 10 mm in patients with a total of 13 cases, abortion in patients with failure of the 2 cases, incomplete abortion in 4 cases, complete abortion in 7 cases, abortion failure probability was 15.4%.The gestational sac size ranged from 10mm to 25mm in a patient with a total of 25 cases, with a miscarriage of failure in a total of 2 patients, incomplete abortion in a total of 2 patients, complete abortion in a total of 21 patients, and a miscarriage probability of failure of up to & 8%.The gestational sac size is greater than 25 mm in patients with a total of 12 cases, the abortion of the failure of the patients in the 4 cases, incomplete abortion in the 2 cases, complete abortion in the 6 cases, the probability of abortion failure was 33.3%.Therefore, there was a significant difference between the three groups in the rate of abortion failure (P<0.05).ConclusionThe process of drug abortion in pregnant women, the use of transvaginal ultrasound monitoring, can monitor the situation of pregnant women with gestational sac in a certain extent, and that the effect of drug abortion, is conducive to the treatment and the subsequent operation, thereby reducing the pain of patients, improve the success rate of miscarriage.
2.Phenylephrine-induced excessive hypertrophy responses in cardiomyocytes from endothelial nitric oxide synthase gene knockout mice
Shumei SHI ; Gang XU ; Huabi WU ; Jianlin LU
Chinese Journal of Tissue Engineering Research 2007;0(15):-
AIM: The study aimed to observe the difference in hypertrophy responses in cardiomyocytes of cultured neonatal endothelial nitric oxide synthase (eNOS) gene knockout mice and wild mice by RNA quantitation detection and cell area estimation. METHODS: The experiment was performed at the Empirical Study Center of Medicine Department in Shunde Profession and Technique Institution in August 2007. ①Male or female C57BL/6 stock neonatal eNOS knockout mice and wild mice aged 1-3 days were selected. ②Cultivated Mouse cardiomyocytes were divided into experimental and control groups 72 hours later. Adrenergic receptor agonist phenylephrine was cultured with cardiomyocytes. DMEM of the same volume as the experimental group was added in the medium in the blank control group. ③DNA, RNA quantitation detection and cell area estimation methods was used to observe the responses contrast in cell proliferation and hypertrophy of cardiomyocytes in cultured neonatal eNOS gene knockout mice and wild mice. RESULTS: ①The oval or rhombus cardiomyocytes were observed 72 hours after phenylephrine incoporation under a microscope. ②RNA fluorescence value increased in cultured neonatal cadiomyocyte and collagenoblast in the experimental group compared to the control group (P