OBJECTIVE:To observe the effects of thalidomide in the growth of the human ovarian cancer cells (OVCAR-3) and HCT-8 as well as the angiogenesis in tumor tissue through building a model of heterograft of nude mice with human tumors. METHODS: After implanting human tumors in nude mice for modeling, the inoculated nude mice were intragastrically administered different dose of thalidomide, with tumor growth condition observed and compared with control group. The diversity of microvessel density(MVD) in tumor tissue was detected by immunohistochemistry. RESULTS: Middle and high dose thalidomide could significantly inhibit the growth of OVCAR-3 human tumor in nude mice(P

Some specific markers are useful of separating and identifying gastric cancer stem cells.They are including gastric stem cells,general tumor stem cells and mesenchymal tissue stem cells.At present,more and more new biomarkers are discovered besides familiar CD133 and CD44.Combined detection helps to separate pure gastric cancer stem cells and promote the therapy of gastric cancer.

Objective To discuss the activity of Toxoplasma gondii and its impact on fetus during pregnancy.Methods Sixty -eight pregnant women whose IgG was positive and IgM was negative for anti - Toxoplasma gondii antibodies were chosen.Their serum and umbilical blood were tested for IgG,IgM,CAg (by ELISA) and DNA (by PCR) of Toxoplasma gondii.The infections of placental samples were tested by the direct smear,homogenate smear and PCR.Results There were 28 positive cases of Toxoplasma gondii IgG antibodies in cord blood,and the vertical transmission rate of IgG in placenta was 41.2%.There were 6 positive cases of Toxoplasma gondii DNA in cord blood,and the incidence of intrauterine infection was 8.8%.There were 9 positive cases of Toxoplasma gondii DNA in placental tissues,and the incidence of intrauterine infection was 13.2%.Conclusions Past Toxoplasma gondii infection during pregnancy may lead to vertical transmission between the mother and infant.

Objective To explore the related factors of the relapse of schizophrenics in unwell developed economic areas.Methods To analyze 718 schizophrenics by single and multiple stepwise regression analyses to explore whether relapse of schizophrenia was related to those factors.Results 2.8％ experienced one time relapse, 14.2％ experienced two times relapse and 83％ three times. The rate of schizophrenic relapse was in turn correlated with treatment compliance, treatment intervention, marriage, clinical features, psychological stress,social support, the way of forming disease, medical expenses and condition of access to medical care. Conclusions More attention should be paid to the factors causing schizophrenic relapse and wider measures of prevention and treatment should be taken.

Objective To study the current situation of diagnosis and treatment of rudimentary uterine horn pregnancy through 4-cases report. Methods We made a clinical study of 4 cases of rudimentary uterine horn pregnancy admitted to our hospital between 1995 and 2005. Results Three patients had been undergone artificial abortion before they were admitted to our hospital. All the patients were asymptomatic. Ultrasonography was made in 3 cases. Two cases were diagnosed as rudimentary uterine horn pregnancy. Three patients were cured with surgery. Conclusions Rudimentary uterine horn pregnancy is rare and with atypical clinical manifestation in the first trimester. Although diagnosis before surgery is difficult, ultrasonography and MRI make a great role. Surgery is the most important therapeutic method.

Objective To investigate the effect of slow-acting antirheumatic drugs on lipid profile and disease activity index in patients with rheumatoid arthritis (RA). Methods Eighty-two patients with RA who met the American College of Rheumatology (ACR) criteria were treated with disease-modifying antirheumatic drugs including methopterin (MTX), salazosulfapyridine (SASP) and hydroxychloroquine sulfate, while seventy-nine healthy volunteers were used for control. All persons were followed up for 12 months. The observations included 28 joint indices score (DAS-28), lipid profile, c-reactive protein (CRP) and erythrocyte sedimentation rate (ESR). Results Compared with the control group before treatment, RA patients exhibited higher serum levels of total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C) and triglycerides. Whereas, high-density lipoprotein cholesterol (HDL-C) was significantly lower. After 12 months treatment, the disease activity index[(6.7±0.6)vs. (2.1±0.9)], ESR [(62±18)mm/h vs. (13±9)mm/h] and CRP [(2.2±0.3 ) mg/L vs. (0. 3±0.2 ) mg/L, all P<0.01] were significantly decreased and HDL-C [1.0±0. 1)mmol/L vs. (1.5±0. 3)mmol/L, P<0. 01] was increased in RA patients. Conclusions RA patients are characterized by an atherogenic lipid profile, which are improved after therapy. Immuno-intervention to control disease activity may reduce the risks for the atherosclerotic process and cardiovascular events in RA patients.

Objective To investigate the information ability of medical personnel from a large general hospital that integrates medical care,teaching and research under the network environment for providing evidence to improve targeted information service in library.Method A self-designed and self-administered questionnaire was distributed through the questionnaire system on hospital office network.Information ability involved in the questionnaire mainly included the ability to precisely retrieve literature in Chinese and foreign language,commonly used retrieval strategies,and screening,evaluation and English full-text access methods.A total of 1 094 medical personnel voluntarily participated and completed the questionnaire,and complete responses were obtained from 1 065 individuals (effective response rate 97.35％).x2 and trend x2 tests were used for data analysis.Results The overall accuracy of precise retrieval information was only 6.1％,and the accuracies of precise retrieval literature in Chinese and foreign language databases were 14.6％ and 14.6％,respectively.Duty,education and working years of the medical personnel had significantly effects on the ability to precisely retrieve literature and the strategies and methods of information retrieval,screening,evaluation and full-text access.Conclusion Medical personnel had certain information ability,but library should carry out relevant training program to improve the medical personnel's information quality and ability further.

Recurrence and metastasis are main causes ofsurgical treatment failure of non-small cell lung cancer.In prevention of non-small cell lung cancer recurrence and metastasis,Chinese medicine has achieved a certain effect. It has been proved by clinical use of Chinese patent drugs, compound modification based on syndrome differentiation, integrated traditional Chinese and western medicine treatment, and molecular mechanism of anti-recurrence and metastasis, though there are still some problems. This article reviews the recent clinical and experimental research status.

Objective To conclude nursing experience of second anastomotic leakage after radical gastric cancer surgery. Methods The present study compared the differences of clinical symptoms between these two anastomotic leakages, and found a way to detect latent anastomotic leakage with an unobvious abdominal sign accurately and opportunely. The study also intervened in the patient′s observation, psychological condition, peritoneal irrigation and nutritional support after surgery. Results The patients were discharged from the hospital after cured with well controlled condition. Conclusions In order to make an early detection and treatment of the second anastomotic leakage, nurses should not only observe the abdominal sign, but also observe the systematic poisoning and concomitant symptoms.

Objective To establish a method of preparing metoprolol succinate sustained-release tablet and its content determination. Methods The formulation was optimized through the orthogonal design test by using release rate of the drug as an indicator.The different batches of metoprolol succinate sustained-release tablets were determined by HPLC. Results The tablets could release drug steadily and slowly as designed,which was similar to imported tablets. The linear range of metoprolol succinate was 10-70 μg?mL-1( r=0.999 8) . Conclusion The releasing rate of metoprolol succinate sustained-release tablet prepared in optimum condition can meet the requirement. This preparation technology is simple, the assay method is rapid, sensitive and reproducible.