OBJECTIVE:To observe the effects of thalidomide in the growth of the human ovarian cancer cells (OVCAR-3) and HCT-8 as well as the angiogenesis in tumor tissue through building a model of heterograft of nude mice with human tumors. METHODS: After implanting human tumors in nude mice for modeling, the inoculated nude mice were intragastrically administered different dose of thalidomide, with tumor growth condition observed and compared with control group. The diversity of microvessel density(MVD) in tumor tissue was detected by immunohistochemistry. RESULTS: Middle and high dose thalidomide could significantly inhibit the growth of OVCAR-3 human tumor in nude mice(P

Objective To investigate the expression of FoxP3 in nasopharyngeal non-keratinizing squamous cell carcinoma and the significance of regulationary T (Treg) cell in the occurrence and development of nasopharyngeal carcinoma .Methods The im-munohistochemistry staining method(SP) was used to detect the expression of FoxP3 in 57 cases of nasopharyngeal non-keratiniz-ing squamous cell carcinoma and 22 cases of nasopharyngeal mucosa with chronic inflammation ,and the expression of CD8 in naso-pharyngeal carcinoma .Results The number of Treg cell with positive FoxP3 in the nasopharyngeal carcinoma group was 105 .05 ± 52 .22 ,which was significantly higher than 6 .35 ± 6 .06 in the nasopharyngeal mucosa with chronic inflammation ,the difference be-tween them showed statistical significance(P<0 .05) .The number of T cells with positive FoxP3 was relevant to gender and the differentiation degree of carcinoma(P<0 .05) .Conclusion The proliferation of Treg cells with positive FoxP3 can inhibit the anti-tumor immunologic function of the patients with nasopharyngeal non-keratinizing squamous cell carcinoma and provide the favor-able environment for the immunologic escape of tumor cells .

Objective To explore the related factors of the relapse of schizophrenics in unwell developed economic areas.Methods To analyze 718 schizophrenics by single and multiple stepwise regression analyses to explore whether relapse of schizophrenia was related to those factors.Results 2.8％ experienced one time relapse, 14.2％ experienced two times relapse and 83％ three times. The rate of schizophrenic relapse was in turn correlated with treatment compliance, treatment intervention, marriage, clinical features, psychological stress,social support, the way of forming disease, medical expenses and condition of access to medical care. Conclusions More attention should be paid to the factors causing schizophrenic relapse and wider measures of prevention and treatment should be taken.

Objective To investigate the authors, the institution and endemic distribution of these authors of Chinese Journal of Orthopaedics, and understand the current situation and problems in the field of Orthopaedics. Methods The authors, the co-authors and the core authors of papers published in Chinese Journal of Orthopedics from 2005 to 2009 were quantitatively analyzed using literature search through CMCI.The distribution of institution, region and highly quantitative organization were also analyzed. Results The 1182 papers were published by the Journal within 5 years. There were 1108 papers with one or more co-authors; the cooperative rate and degree were 93.74% and 5.02, respectively. 74 papers were written by single author, which accounted for 6.26% of all papers. 615 authors (75.18% of all first authors) published only one paper. There were 295 (24.96%) papers from 67 (8.19%) core authors. 601 (50.85%) papers were from 20 high-productivity institutes. The top three high-productivity institutes were Tianjin hospital, Beijing Jishuitan hospital and Nanjing Drum Tower hospital of Nanjing University Medical School. The number of papers were 89 (7.53%), 75 (6.35%) and 43 (3.64%), respectively. The number of papers from the teaching hospital,provincial or municipal hospitals, military hospitals were 722 (61.08%), 226 (19.12%) and 157 (13.28%),respectively. According to the distribution of Province, Beijing was the first of place with 264 (22.34%) papers, followed by Shanghai by 160 (13.54%) papers and Tianjin by 136 (11.51%) papers. The distribution range of the authors has been expanded to five countries. Conclusion The influence of the Chinese Journal of Orthopaedics has greatly increased. Authors of the Chinese Journal of Orthopaedics have a highly cooperative rate. There is a group of active and talented core authors who has a great influence on the journal. But the number of core authors and the papers published by core authors need to be expanded.

Objectives To provide outline for the Research & Development department of hospitals making research strategy by analyzing the quantity of publications and their cited status for the top 20 hospitals in SCI and MEDLINE, and the top 100 hospitals in domestic publications in 2008. Methods The number of publications and their cited times for the first 20 hospitals in SCI and MEDLINE, and the top 100hospitals in domestic publications in 2008 were analyzed with bibliometric analysis. Results Compared with 2007, the increased number and ratio of publications in SCI and MEDLINE for the top 20 hospitals were 1050, 51.19% and 658, 15.04%, respectively; for top 100 hospitals, the average decreased number of domestic publications was 2.08, and the number of cited publications was increased 202.52 and the increased ratio was 13.51%. Conclusions The SCI publications from top 20 hospitals increase sharply, the increase of the MEDLINE articles from the top 20 hospitals is slowly. The negative growth is showed in the number of domestic articles for the top 100 hospitals. In addition, the increase of cited articles is slowly and disproportion.

Objective To investigate the effect of slow-acting antirheumatic drugs on lipid profile and disease activity index in patients with rheumatoid arthritis (RA). Methods Eighty-two patients with RA who met the American College of Rheumatology (ACR) criteria were treated with disease-modifying antirheumatic drugs including methopterin (MTX), salazosulfapyridine (SASP) and hydroxychloroquine sulfate, while seventy-nine healthy volunteers were used for control. All persons were followed up for 12 months. The observations included 28 joint indices score (DAS-28), lipid profile, c-reactive protein (CRP) and erythrocyte sedimentation rate (ESR). Results Compared with the control group before treatment, RA patients exhibited higher serum levels of total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C) and triglycerides. Whereas, high-density lipoprotein cholesterol (HDL-C) was significantly lower. After 12 months treatment, the disease activity index[(6.7±0.6)vs. (2.1±0.9)], ESR [(62±18)mm/h vs. (13±9)mm/h] and CRP [(2.2±0.3 ) mg/L vs. (0. 3±0.2 ) mg/L, all P<0.01] were significantly decreased and HDL-C [1.0±0. 1)mmol/L vs. (1.5±0. 3)mmol/L, P<0. 01] was increased in RA patients. Conclusions RA patients are characterized by an atherogenic lipid profile, which are improved after therapy. Immuno-intervention to control disease activity may reduce the risks for the atherosclerotic process and cardiovascular events in RA patients.

Objective To conclude nursing experience of second anastomotic leakage after radical gastric cancer surgery. Methods The present study compared the differences of clinical symptoms between these two anastomotic leakages, and found a way to detect latent anastomotic leakage with an unobvious abdominal sign accurately and opportunely. The study also intervened in the patient′s observation, psychological condition, peritoneal irrigation and nutritional support after surgery. Results The patients were discharged from the hospital after cured with well controlled condition. Conclusions In order to make an early detection and treatment of the second anastomotic leakage, nurses should not only observe the abdominal sign, but also observe the systematic poisoning and concomitant symptoms.

Objective To investigate the information ability of medical personnel from a large general hospital that integrates medical care,teaching and research under the network environment for providing evidence to improve targeted information service in library.Method A self-designed and self-administered questionnaire was distributed through the questionnaire system on hospital office network.Information ability involved in the questionnaire mainly included the ability to precisely retrieve literature in Chinese and foreign language,commonly used retrieval strategies,and screening,evaluation and English full-text access methods.A total of 1 094 medical personnel voluntarily participated and completed the questionnaire,and complete responses were obtained from 1 065 individuals (effective response rate 97.35％).x2 and trend x2 tests were used for data analysis.Results The overall accuracy of precise retrieval information was only 6.1％,and the accuracies of precise retrieval literature in Chinese and foreign language databases were 14.6％ and 14.6％,respectively.Duty,education and working years of the medical personnel had significantly effects on the ability to precisely retrieve literature and the strategies and methods of information retrieval,screening,evaluation and full-text access.Conclusion Medical personnel had certain information ability,but library should carry out relevant training program to improve the medical personnel's information quality and ability further.

Objective To establish a method of preparing metoprolol succinate sustained-release tablet and its content determination. Methods The formulation was optimized through the orthogonal design test by using release rate of the drug as an indicator.The different batches of metoprolol succinate sustained-release tablets were determined by HPLC. Results The tablets could release drug steadily and slowly as designed,which was similar to imported tablets. The linear range of metoprolol succinate was 10-70 μg?mL-1( r=0.999 8) . Conclusion The releasing rate of metoprolol succinate sustained-release tablet prepared in optimum condition can meet the requirement. This preparation technology is simple, the assay method is rapid, sensitive and reproducible.

Liver cirrhosis is the advanced stage of various chronic liver diseases,and its complications include portal hypertension,esophageal and gastric varices,hepatocellular carcinoma,etc. Transient elastography( TE),a noninvasive method for liver stiffness measurement( LSM),has been used widely to detect liver fibrosis qualitatively and quantitatively. Recently,correlations were observed for LSM with liver cirrhosis and its complications. This article reviewed the studies focused on these correlations,as well as the clinical application of TE.