Objective To investigate the clinical effects of the surgery of mastoid muscle periosteum valvuloplasty after modified radical mastoidectomy treatment of cholesteatoma otitis media.Methods 52 cases (52 ears)cholesteatoma otitis media patients for the observation group were collected.The patients in the observation group were treated by mastoid muscle periosteum valvuloplasty after modified radical mastoidectomy.49 cases (49 ears) for the control group were treated by concomitant mastoidectomy and the classic conchaplasty cavity angioplasty.Results Patients were followed up for 1 year.38 ears were dry in control group(77.6％),8 ears were recurrenced(16.3％),regular clean-up of surgical cavity at least 3 months postoperative.50 ears were dry in observation group (95.2％),dry ear rate was significantly higher (x2 =12.374,P =0.000) ;1 ears was recurrenced(2.4％),the recurrence rate was significantly reduced (x2 =11.966,P =0.000) ; Operative cavity after complete epithelialization no longer need to be cleaned regularly; No postoperative complications (such as periosteal flap or pinna cartilage of the mastoid muscle necrosis,facial paralysis,dizziness)were occurred,the prognosis was good.Conclusion The surgery of mastoid muscle periosteum valvuloplasty after modified radical mastoidectomy treatment of otitis media with cholesteatoma is safe and effective,which is worthy of promotion.

Objective To explore the effect of different sleeping state and body position nursing on the surface temperature, heart and lung function, and development of premature infants. Methods A total of 160 cases of premature infants were selected as research objects, divided into active-prone group, active-supine group, quiet-prone group, quiet-supine groupwith random number tables. Each group on the basis of conventional nursing care was given the above position. Respiratory frequency (RR), heart rate (HR), oxygen saturation (SpO2), RR deviation, HR deviation and surface temperature were observed before and after nursing. After continuous nursing for 7 days, the differences of development indexes of each group were also observed. Results Surface temperature of forehead, right flank, right forearm, right leg were (36.4 ± 0.2), (36.4 ± 0.3), (35.6 ± 0.3),(34.4 ± 0.5)℃in group C, which were significantly higher than those in the other groups (F=2.89-3.31, P<0.05). HR, RR, HR-deviation, RR-deviation and its change value in group C were (128.5 ± 1.8) beats/min, (34.6 ± 2.2) beats/min, (9.1 ± 1.2)%, (212.5 ± 31.5)%, which were significantly lower than those in the other groups (F=4.61-4.96,P < 0.05), and SpO2 change value was 0.027±0.001, which was significantly higher than those in the other groups (F=3.65,P<0.05). Weight gain, the length growth, head circumference growth and upper arm around growth in group C and A in preterm infants after 7 days of continuous nursing were (118.6±6.3) g, (11.3±1.2) mm, (6.4±0.4) mm, (4.4± 0.2) mm and (110.3 ± 5.7) g, (11.0 ± 1.1) mm, (6.0 ± 0.5) mm, (4.2 ± 0.3) mm, which were significantly better than (74.5 ± 4.4) g, (6.9 ± 0.8) mm, (4.5 ± 0.7) mm, (3.2 ± 0.2) mm of active-supine group and (78.6 ± 4.9) g, (7.6±0.7) mm, (4.8±0.6) mm, (3.5±0.3) mm of quiet-supine group (F=3.57, 4.98,P<0.05). And the above indicators of quiet-prone group were higher than that of active-prone group (P<0.05). Conclusions The quiet-prone position can improve the heart and lung function and development of premature infants.

Femtosecond laser has been widely used in ophthalmology for its high precision, safety and minimal damage to tissues, and now its application in the corneal refractive surgery, corneal stromal ring implantation and cataract surgery has been more mature.In recent years, it has been actively developed and applied in penetrating keratoplasty, anterior lamellar keratoplasty and corneal endothelial transplantation, and some achievements have been obtained in experimental research and clinical application.While femtosecond laser promotes the development of corneal transplantation surgery, there are still some problems such as limited indications, uncertain efficacy, expensive equipment, lack of big data support and intelligence in personalized design.Continuous optimization and improvement are still needed to ensure postoperative vision, reduce postoperative complications, simplify parameter design and reduce consumption costs.In this paper, the indications, parameter design, clinical effect and limitations of femtosecond laser in keratoplasty were reviewed.

Aspirin is one of the major drugs used for preventing atherothrombotic vascular diseases,but not effective to all patients or so-called aspirin resistance.However,the mechanisms of this resistance still remains to be elucidated.The recent progresses is reviewed in the therapeatic effect of aspirin and mechanisms of the drug resistance.

AIM:To explore the effects of panax notoginsenosides(PNS)on bone histomorphometry in rats with hyperlipidemia-induced osteoporosis.METHODS:Thirty 3-month-old Sprague-Dawley rats were randomly divided into three groups with 10 per group as follows.Control group was intragastrically given distilled water at the dose of 5 mL?kg-1?d-1 for 20 weeks;Model group was intragastrically given lipid emulsion at the dose of 5 mL?kg-1?d-1 for 20 weeks;PNS preventing group was intragastrically given lipid emulsion at the dose of 5 mL?kg-1?d-1 in the morning and PNS at the dose of 5 mL?kg-1?d-1 at afternoon for 20 weeks.At the end of the experiment,the right longitudinal proximal tibial metaphyseal parameters of rats were performed undecalcifiedly and used for bone histomorphometry analysis.And the blood serum contents of FFA,TG,TC,HDL-C and LDL-C were measured by biochemical analysis.RESULTS:Compared with model group,the blood serum contents of FFA,TC and LDL-C were decreased and HDL-C was increased significantly(P

OBJECTIVE:To optimize the emulsifying technology of Compound kuhuang cream. METHODS: The factors influencing the stability of the cream were optimized by orthogonal experiment with the thickness of the oil layer obtained after centrifugalization and substratification of cream as index. RESULTS: The optimum condition was obtained when the ratio of oil-phase stearine∶glyceryl monostearate∶lanoline∶liguid paraffin∶white vaseline was 4∶1∶2∶2∶1,the proportion of emulsifier was 4%, the hydrophile-lipophile balance (HLB) was 13 and the emulsion temperature was 80℃. CONCLUSION: The optimized emulsifying technology is stable and feasible and provides theoretic basis for the industrial production of Compound kuhuang cream.

AIM: To investigate the ability of leupeptin, TLCK and lactoferrin on tryptase release from human colon mast cells. METHODS: Human mast cells were dispersed from colon tissue with collagenase and hyaluronidase, and were challenged with stimulus for 15 min at 37 ℃. Tryptase concentrations were measured with a sandwich ELISA procedure. RESULTS: Leupeptin, TLCK and lactoferrin were not able to provoke significant tryptase release from human colon mast cells. But they were able to inhibit anti-IgE and calcium ionophore (CI)-induced tryptase release in a concentration-dependent manner. Preincubation of inhibitors of leupeptin and TLCK with cells for 20 min before challenging with anti-IgE failed to enhance their inhibitory actions. The extent of inhibition by leupeptin was increased when colon mast cells were preincubated for 20 min before CI being added. However, the same treatment failed to improve the action of TLCK. CONCLUSION: We found for the first time that leupeptin, TLCK and lactoferrin were able to inhibit anti-IgE and calcium ionophore-induced tryptase release from human colon mast cells, which may indicate a potential of a novel therapy for the treatment of inflammatory bowel disease or other mast cells-related diseases.

Many newly discovered interleukins have been implicated to play an important role in the pathogenesis of the allergic diseases, for example, human interleukin (IL)-17, a T-cell derived cytokine; interleukin (IL)-18, an interferon (IFN)-gamma-inducing cytokine; interleukin (IL)-23, produced by activated dendritic cells, and interleukin (IL)-25, a recently described T helper 2 (Th2) cell-derived cytokine. Understanding their characteristic and roles in diseases may help us to know better the mechanism of the allergic diseases and develop the strategy for treating the disease. [

Objective To determine the content of calycosin-7-?-O-D-glucopyranpside in Danggui Buxie Dropping Pills.Methods HPLC method was used. The chromatographic column was YMC-C18(150?4.6 mm, 5 ?m), the mobile phase was methanol and water(40 ∶60), the detection wavelenth was 235 nm, column temperature at 20 ℃and flow rate was 1.0mL/min.Results Calycosin-7-?-O-D-glucopyranpside was separated well with other components and was in good linearity in the range of 0.02～0.80 ?g. The average recovery was 97.62 %and RSD=1.06 %.Conclusion The method is rapid and accurate and can be used for quality control of Danggui Buxie Dropping Pills.

Objective To investigate the effectiveness of early minimal enteral feeding′s prevention and cure of feeding intolerance of very low birth weight infants (VLBWs).Methods A total of 45 VLBWs from the neonatal intensive care unit(NICU) of the second people′s hospital of Shantou,were included,from 2011 Jan to 2012 Dec. 23 infants in the intervention group which were hospitalized in 2012,received early minimal enteral feeding (MEF), while 22 infants in the control group which were hospitalized in 2011,received routine feeding .The incidence of feed-ing intolerance of the two groups was observed .Results The incidence rate of feeding intolerance was 21.74%(5 cases) in the intervention group and 72.73%(16 cases) in the control group.The incidence rate of feeding intol-erance of the intervention group was lower than that of the control group obviously and the difference was statistical significance(P<0.05).The time to achieve full enteral feeding of the intervention group [(28.22 ±1.97)days] was significantly shorter than that of the control group [(34.23 ±3.10)days](P<0.05).Conclusion An early MEF is beneficial to reduce the occurrence rate of feeding intolerance ,achieve full enteral feeding sooner ,decrease the length of hospital stay and improve the life quality of VLBWs .