Humans ; Lead ; urine ; Lead Poisoning ; diagnosis ; urine ; Spectrometry, Fluorescence ; methods ; Spectrophotometry, Atomic ; methods

The article introduced the recent progress of tissue-engineered trachea.The problems encountered in the field and the study direction in the future are also mentioned.

Objective To investigate the role of peripheral blood lymphocyte apoptosis through Fas/ Fas ligand pathway in the development of viral myocarditis. Methods Twenty-five viral myocarditis (VMC) and 25 healthy children were chosen for study. The apoptosis percentage in peripheral blood iymphocytes was quantitatively examined, flow cytometry with Annexin V/PI double parameter was used. And the expressions of Fas and Fasl protein in peripheral blood lymphocytes were analyzed with flow cytometry. Results The percentage of apoptosis in peripheral blood lymphocytes in children with VMC [(0. 6976 ± 0. 1109)%] was significantly higher than that of healthy children [(0. 1288 ± 0. 1069) %] (t = 18.459, P ＜ 0.001). Moreover, the apoptosis cell percentage showed a significant positive correlation with the serum levels of myocardium enzyme (creatine kinase-MB, lactate dehydrogenase, creatine kinase, hydroxybutyrate dehydrogenase)(r =0. 817 ,P ＜0. 01 ;r =0. 785 ,P ＜0.01 ;r =0. 726 ,P ＜0. 01 ;r =0. 712 ,P ＜0. 01). The expression of Fas and Fasl protein in VMC patients(2. 8804 ± 0. 3020,4. 4496 ± 0. 4836) were higher than those of health children(2. 1300 ±0. 6200,0. 1238 ±0. 0384) ,which had significant difference(t = 17. 321 ,P ＜0. 001 ;t' =44. 577, P ＜ 0. 001). The apoptosis percentage of peripheral blood lymphocytes in children with VMC showed a significant positive correlation with the Fas and Fasl protein expression in peripheral blood lymphocytes(r =0. 972, P ＜ 0. 01; r = 0. 958, P ＜ 0. 01). Conclusion Apoptosis percentage of peripheral blood lymphocytes increases among children with VMC, probably because immune response decreased or it is related with the degree of myocardium damaged. Fas and Fasl play an important role in apoptosis of peripheral blood lymphocytes in children with VMC.

Recently, it is reported that regulatory T cells (Tregs) can be reprogrammed into a novel population [interleu?kin (IL)-17+Foxp3+T cells] phenotypically and functionally resembling Th17 cells under complicated cytokine circumstanc?es. IL-17+Foxp3+T cells are characterized by production of IL-17 and expression of retinoic acid receptor related orphan re?ceptor (ROR)γt, demonstrating dual functions in immune response and providing novel insight into the interconnection be?tween Tregs and Th17 cells. In this review, we lay emphasis on the phenotype features, origination, differentiation and the pleiotropic functions of IL-17+Foxp3+T cells. Furthermore, we summarized the functions of IL-17+Foxp3+T cells in inflam?matory disease and tumor microenvironment.

Objective To study the effects of traditional Chinese medicine Zuojin Pill on gastrointestinal system.Methods Through ig giving 0.1％ methyl orange solution,the gastric residual rate was calculated,and the effect of Zuojin Pill on gastric emptying in mice was observed.By ig administration of 5％ carbon powder,the advance rate of carbon powder was calculated,and the effect of Zuojin Pill on intestinal motility in normal mice and the antagonistic effect on intestinal motility hyperfunction induced by neostigmine were observed.Effect of Zuojin Pill on contraction of isolated guinea pig ileum induced by histamine was observed.After D-xylose solution was ig given to rats,the serum xylose level was determined 1 h later,and the effect of Zuojin Pill on rat intestinal absorption was observed.The anti-diarrhea effect of Zuojin Pill was observed by giving castor oil to mice.At the same time,we have made a comparison between Zuojin Pill and Wuji Pill-berberine,to evaluate the rationalization of Zuojin Pill.Results It was obvious in experimental results that Zuojin Pill had a regulating effect on gastrointestinal system.Zuojin Pill dose-dependently inhibited the gastric emptying.Zuojin Pill demonstrated more effective suppression on the accelerated movement induced by neostigmine,but did not inhibit normal intestinal transit in mice.Zuojin Pill markedly inhibited sperm motility in mice.Zuojin Pill concentration-dependently inhibited the contraction intensity of isolated ileum muscles in guinea pigs induced by histamine.Zuojin Pill markedly inhibited diarrhea induced by castor oil.Compared with Wuji Pill-berberine,the effect of Zuojin Pill on gastrointestinal system was more visible in the same dose.Conclusion Zuojin Pill has obvious regulation effect on the gastrointestinal tract,there are indications that the application of Zuojin Pill to clinic is scientific and reasonable.

Objective To investigate Shuxuening injection combined with deproteinized extract of calf blood on serum IGF-1, IL-1 and ICAM-1 level in patients with cerebral infarction recovery stage.Methods 120 patients with cerebral infarction were collected.According to the different drug treatment, 60 cases in each group were given corresponding drug treatment, on the basis of control group, the calf blood extract injection was given in experimental group.After the end of treatment, all patients blood rheology, IGF-1, IL-1 and ICAM-1 levels were tested.Results Compared with control group, the indexes of the patients in experimental group improved more significantly, whole blood viscosity, red blood cell pressure volume, platelet aggregation rate decreased significantly(P<0.05); serum IGF-1 levels in experimental group decreased significantly(P<0.05).The levels of serum IL-1, ICAM-1 in experimental group decreased significantly(P<0.05).Conclusion Shuxuening injection combined with deproteinized extract of calf blood can significantly reduce serum IGF-1, IL-1 and ICAM-1 levels in patients with cerebral infarction recovery stage, improve blood flow, reduce blood viscosity.

The implementation of the Malpractice handling Regulations, which has set new and higher demands on medical institutions and medical workers, will have a certain impact on hospital administration and patient care. Medical institutions and medical workers must be highly aware of this. The entire staff of each hospital must study the Regulations carefully and try to obtain an objective understanding of it. Management of medical institutions must be strengthened, weak links identified, measures formulated and regulations perfected. Medical workers ought to strive for better professional skills, and supervision and management of medical records ought to be intensified.

Objective To establish a database for models of teeth, which could provide a new technology for personal identification based upon teeth and bite mark for forensic medical practice. Method The (X, Y) digital coordinates were established based up on materials concerning shape and arrangement of the teeth by analyzing samples of 400 models of teeth. The position of every tooth's key point corresponding to the origin was obtained, thus, the characters of tooth and dental arch could be calculated rapidly according to Borland C ++ 5.0 computer language under DOS system. Results The models of teeth could be identified automatically. Among the correlative precision indexes, the length was 1.3mm, the angle was 3?. It seems reasonable to select 12-14 teeth points for studying, which needed about 5-7 complete tooth marks. Conclusion This method is one of the bases in studying personal identification relating to tooth and bite mark.

Objective To discuss the condition in which ricochet bullet wound is formed, study its ballistic characters and ability to damage in the body, and establish features for the diagnosis of ricochet bullet wound. Method We shot four Yorkshire pigs by selecting No.54 pistol (made in China) and No.56 semiautomatic rifle with No.51 pistol bullet and No.56 rifle bullet, using quern stone as ricocheting target, copied 12 cases of ricochet bullet wound models and observed the characters of entrance wound, wound cavity and cartridge point. Results The formation of ricochet bullet lies with the properties of the target, the shape and velocity of the bullet, angle of incidence and angle of ricochet; the angle of ricochet formed by sharp bullet with high velocity is smaller than the angle by the round bullet with low velocity under the same circumstance; the wound does not produce an evident temporary cavity; six features for the diagnosis were established according to experimented results. Conclusion The correct diagnosis of ricochet bullet wound is of practical value to judging direction and distance of shot, trajectory reconstruction, analysing purpose of shot and evaluation of injury degree.

Objective To probe the mechanism of Warlker-256 cell apoptosis resulted from permanent and strong magnetic field. Method The amplification, rearrangement, deletion, transcription and expression of P53 were detected in 320 rats treated by magnetic field and 80 controls, using Dot Blot of DNA and RNA, Southern and Northern blot, and immunohistochemical technologies. Results P53 is wild type in Warlker-256 cells, no amplification, rearrangement and deletion were found in either magnetic treated group or control group. The transcription and expression of P53 is significantly enhanced in the treated group versus the control(P