Anti-Infective Agents ; therapeutic use ; Humans ; Sinusitis ; drug therapy

Based on the theory of tumor angiogenesis,the vascular system is necessary for the advance and metastasis of the tumor.To inhibit or even cure the tumor,the researchers all over the world have made a great many of studies on the mechanism of tumor angiogenesis and have acquired some achievements.Based on the findings,a lot of factors are involved in tumor angiogenesis,and they constitute the complex regulating network through interaction at the molecular and cellular level.

Allergens ; immunology ; Child ; Desensitization, Immunologic ; Humans ; Rhinitis, Allergic, Perennial ; immunology ; therapy ; Treatment Outcome

Asthma ; immunology ; Humans ; Rhinitis ; immunology

China ; Chronic Disease ; Humans ; Phenotype ; Rhinitis ; genetics ; immunology ; Sinusitis ; genetics ; immunology

Objective: This study aims to investigate the protein expressions of cyclooxygenase-2 (Cox-2) and hypoxia-inducible factor 1 alpha (HIF-1α) and their correlations with the clinicopathological features of osteosarcoma and tries to reveal the potential mechanisms for the formation and progression of osteosarcoma. Methods: We screened 59 typical samples of osteosarcoma tissues without os and decalcification treatment. They were grouped according to the clinicopathologic characteristics and were followed up post surgery. Expression of Cox-2 and HIF-1 alpha were detected by immunohistochemistry. The results were evaluated by X2 analysis and Spearman rank correlation analysis. The postoperative survival rates were calculated by Kaplan-Meier survival curve and the differences between groups were analyzed by Log-Rank method. Results: The positive expression rates of COX-2 and HIF-1α in osteosarcoma were 69.49% and 38.98%, respectively, which were significantly different compared with negative expression in control group (P<0.01 and 0.05). The expression of Cox-2 in osteosarcoma positively correlated with the clinical stage (P<0.05) and the expression of HIF-1α correlated with the age of patients (P<0.01). The expressions of Cox-2 and HIF-1α were closely associated with the histological classifications. In COX-2- and HIF-1α-positive groups, the total positive rates (16.67% and 0%) of intraosseous well-differentiated and parosteaosteosarcoma were significantly lower than that of any others (73.33%, and 43.18%), respectively. The difference was significant (and P<0.01 and P<0.05). The expression of Cox-2 had positive correlation with HIF-1alpha. The positive co-expression of both HIF-1α and Cox-2 was 38.98%. There was 30.50% osteosarcoma tissues had no expression of both Cox-2 and HIF-1 alpha. The positive expression rates of HIF-1α in Cox-2 - positive and Cox-2 - negative groups were 56.10% and 0%, respectively. The difference was significant (P<0.01). The postoperative survival period was negatively associated with the expression of Cox-2 and HIF-1α. The postoperative accumulative survival rates were significantly lower in Cox-2 - and HIF-1 alpha - positive patients compared with those in Cox-2 - and HIF-1 alpha-negative patients (P<0.05 and P<0.01, respectively). Conclusion: Cox-2 and HIF-1α were over-expressed in osteosarcoma. Their expressions are related with the formation and progression of tumor and may have synergistic effects in prompting the malignant development of osteosarcoma. The patients with positive expression of Cox-2 and HIF-1α have lower postoperative accumulative survival rates. Their expressions may be useful prognostic factors for osteosarcoma.

Objective To investigate the value of intermediated cutting approach in the total thyroidectomy.Methods The clinical data of patients with total thyroidectomy from January 2009 to December 2011 were analyzed retrospectively.Patients in group A(n =134) were underwent thyroidectomy through intermediate approach,whereas patients in Group B (n =187) were underwent traditional total thyroidectomy.The analysis index included operation periods,operative bleeding amount,the incidence of hypocalcemia and rate of laryngeal nerve injure.Results The operation periods in Group A was shorter than that in group B ((65.4 ± 8.7) second vs.(90.1 ± 11.3) min,t =22.195,P ＜ 0.01).The amount of operative bleeding in group A was,less than that in group B ((20.5 ± 5.7) ml vs.(50.8 ± 11.4) ml,t =31.294,P ＜ 0.01).The incidence rates of temporary recurrent laryngeal nerve injure and the temporary hypocalcemia rate in group A and group B were (0.74％ vs.5.88％,10.44％ vs.24.59％ respectively,x2 =5.72,10.28 respectively,P ＜ 0.05).No permanent hypoparathyroidism or permanent recurrent laryngeal nerve injure case occurred in both groups.Conclusion Intermediate cutting approach was an effective and safe method in terms of total thyroidectomy.

Objective To describe the special ramp lesion, which involves the capsulomeniscal region of posterior horn of medial meniscus and most commonly occurs in association with anterior cruciate ligament(ACL) injury, pre- and intra-operative diagnosis methods, arthroscopy repair technique and follow-up results of arthroscopy second-look & MR review. Methods From April 2002 to April 2005, 89 cases of ACL injury with ramp lesions are enrolled in this series, among them 85 need operation repairing. Arthroscopic ACL reconstruction with hamstring or B-PT-B autograft or allograft and all-inside medial meniscus repair through 2 posteromedial portals and transcondylar portal have been performed. Generally, the ramp lesion accounts for 26.7% of ACL injury, 30.5% for chronic and 19.5% for acute injury respectively. Results Of the repaired ramp lesion cases, 75 cases(88.2%) are available for follow-up, with the duration from 5 to 41 months (mean, 20.2 months). 25 cases underwent arthroscopic second-look and 21 cases received MR evaluation. The study include subjective symptom, clinical examination, and arthroscopic second-look as well as MR review. All of the repaired cases present normal subjective and objective evaluations, and all the 25 second-look cases show completely healing. Of the 21 MR follow-up cases, 18 completely healed, 3 partial healed. Conclusion About 1/3 ACL injuries are associated with medial meniscus ramp lesion, and the morbidity is higher in old injuries than acute injuries. For this combined lesion, one stage ACL reconstruction and meniscus repair was recommended. All-inside meniscus repair is a satisfactory technique for ramp lesion and can get high healing rate.

Asthma ; immunology ; Congresses as Topic ; Humans ; Hypersensitivity ; immunology ; United States

Background The special pathological change of diabetic retinopathy(DR)is microvascular disorder.Angiopoietin-like protein 2(Ang-2)is a new protein associated with genesis of blood vessels.Quercetin has multiple pharmacological action,including improving the microcircularion and the permeability of blood capillary.However,the action mechanism of Ang-2 on DR was unclear.Objective The present study was to investigate the effects of quercetin on Ang-2 and its receptor Tie2 expression in retina with diabetes mellitus.Methods Sixty clean male Wistar rats were randomized into 7 groups and 10 rats for each group,and 10 rats served as blank control group.Streptozotocin of 35 mg/kg was intraperitoneally injected in 60 rats to establish the diabetic models.Quercetins encapsulated by liposome with the doses of 50,150 and 250 mg/(kg · d)(3-5 ml)were used to gavage in different groups of models for 12 weeks,and normal saline solution and calcium dobesilate were used at the same fashion as the negative control group and positive control group,respectively.Twelve weeks later,the animals were sacrificed and retinas were isolated.Expressions of Ang-2 protein and Tie2 mRNA in retinas were detected by ELISA and RT-PCR,respectively.The usage and rearing of the animals complied with the Regulations for the Administration of Affair Concerning Experimental Animals by State Sciences and Technology Commission.Results ELISA showed that the A450 of Ang-2 in 150 and 250 mg/(kg · d)quercetin groups was 0.796±0.057 and 0.842±0.043 respectively and was lower than that negative group(1.012±0.046),showing statistically significant differences(q =2.95,2.698,P＜0.05).RT-PCR assay showed that expression of Tie2 mRNA(Tie2 mRNA/GAPDH mRNA)in retinas was 0.712±0.092 and 0.821±0.087,presenting statistically significant differences in comparison with negative group(1.182±0.098)(q =3.497,2.852,P＜0.05).The expression levels of Ang-2 and Tie2 mRNA in retina were lowest in 150 mg/(kg · d)quercetin group.Conclusions Quercetin can improve the retinal microcirculation by downregulating the expressions of Ang-2 and its receptor in early period of diabetic rats.