Lung transplantation is now considered one of the effective management of end stage pulmonary diseases. This review presents the recent progress of primary graft failure in lung preservation, such as low temperature, ventilation, etc. The purpose is to provide some valid methods in clinical practice.

The catabolic response to severe injury , burn, inflammatory ,acute pancreatitis and operation is characterized by whole-body protein loss, mainly reflecting increased breakdown of muscle proteins. Glucocorticoids and various proinflammatory cytokines are important regulators of muscle proteolysis in stressed patients. In recent years, three types of treatments have been used to reduce or prevent the catabolic response to injury and sepsis: ①nutritional, ②hormonal, and ③pharmacologic. This article review the catabolic response and the corresponding interruption under the stress conditions.

Objective To study the effect of adenovirus type 3I,7b on the expressions of mRNA and protein of transforming growth factor-beta 1(TGF-?1) in human embryonic lung fibroblast cells.Method The expression of mRNA and protein of TGF-?1 were determined in human embryonic lung fibroblast cells before and after being infected by adenovirus type 3I,7b and in normal fibroblast cells with enzyme-linked immunosorbent assay and in situ hybridization.Results The mRNA and protein of TGF-?1 expression in human embryonic lung fibroblast cells increased siginificantly after being infected by adenovirous type 3I,7b compared with those in normal fibroblast cells(Pa0.05).Conclusion Lung fibroblast cells and TGF-?1 may play some roles in pathophysiological processes of viral pneumonia.

AIM: To investigate the effect of curcumin on the binding activity of hepatic nuclear factor-kappa B(NF-?B) and the expression of peroxisome proliferator-activated receptor-?(PPAR-?) in rats with liver fibrosis induced by carbon tetrachoride. METHODS: A total of 60 clean male rats were randomly and averagely divided into group A,B,C,D,E and F.The rats in group A served as normal controls,while those in other five groups were injected subcutaneously 40% CCI_4 for seven weeks to induce the model of liver fibrosis.After seven weeks,the rats in group C,D,E,F were intragastrically administered with 50 mg/kg silibinin,100 mg/kg cur,200 mg/kg cur,400 mg/kg cur once per day for six weeks,respectively.HE staining was used to observe the pathological changes of liver tissues under light microscope,and immunohistochemistry and reverse transcriptase polymerase chain reaction(RT-PCR) were performed to detect the activity of NF-?B,PPAR-? and mRNA expression of PPAR-?.RESULTS: The inflammatory and fibrotic degrees were obviously alleviated in group C,D,and E compared with group B.The expression of NF-?B p65 was significantly decreased in liver tissue in group C,D and E,compared with model control group B(P

0.05).②Gabexate mesilate significantly reduced the frequency of contraction (P0.05).③High dose gabexate mesilate could markedly reduce the motility index ( P

Objective To evaluate the standardization of diagnosis and therapy for stage I epithelial ovarian cancer.Methods Retrospective study of 146 cases of ovarian cancer admitted in our hospital. All of cases had postoperative pathological diagnosis.Results 146 cases of epithelial ovarian cancer from Jan 1980 to Jun 2002. 32 of them were stage I cases (21 92%). The average age was 45 32. The first four symptom were self touching the mass, abdomen bulge,abdomen pain and oppress symptoms. All of 29 cases accepted surgery and chemotherapy in our hospitalexcept that 3 cases.4/32 lost touch ,3/32 died of other diseases, 5/32 recurrence, 3/32 died of cancer.Five-year survival rates were 92 87%, ten-year survival rates were 83 90%. Conclusions Prognosis between early and advanced ovarian cancer is obvious difference. If the diagnosis and therapy for epithelial ovarian cancer is early,and survival rates is highly.

Objective To study the relation between protein kinase C(PKC) and coronary heart disease.Methods To measure the activity of PKC and its inhibitor(PKCI) in platelets,the activity of PKC in erythrocytes from 43 patients with unstable angina pectoris(UAP),45 patients with acute myocardial infarction (AMI),and 42 healthy controls(HC),respectively.Results The PKC activity in platelet membrane from UAP and AMI patients was higher than that from HC.The PKC activity in plalelet cytosol from UAP and AMI patients was lower than that from HC.The PKC activity in platelet cytosol from UAP patients was lower than that from AMI patients.The PKC activity in erythrocyte membrane from UAP and AMI patients was higher than that from HC.The PKC activity in erythrocytes cytosol from UAP and AMI patients was lower than that from HC.Conclusion PKC is likely to be associated with the pathogenesis of coronary heart disease.

Objective To investigate the effect of alendronate combined with calcitriol on bone metabolism in the treatment of postmenopausal osteoporosis patients.Methods66 postmenopausal osteoporosis patients from Cancer Hospital of Hangzhou were selected and randomly divided into the control group and the study group.33 cases in the control group were treated by compound calcium amino acid chelate.33 cases in the study group were treated by compound calcium amino acid chelate, alendronate and alfacalcidol.All patients were taking the drug for 48 weeks.The bone mineral density (BMD) of lumbar vertebrae between 4 and 1, Bone alkaline phosphatase (BALP) and bone metabolism index of left femur (BMD) Type I collagen C end peptide (CTX), acid phosphatase (TRAP), parathyroid hormone (PTH) and clinical curative effect were compared.ResultsAfter treatment, the clinical effective rate of the study group (93.94%) was higher than that of the control group (72.73%), the difference was statistically significant (P<0.05).Compared with the control group,the level of bone mineral density of lumbar spine was increased from 1 to 4, and the level of TRAP, CTX, PTH and BALP were increased,the difference was statistically significant (P<0.05).There was no difference in adverse effects between the two groups.ConclusionThe combination of the application of sodium hyaluronate and the combination of the method can effectively improve the bone mineral density and bone metabolism in postmenopausal women with osteoporosis, and improve the clinical efficacy.

Objective To observe the effects of treating knee osteoarthritis(KOA)combined with synovitis with Gubiqing,and discuss its mechanism.Methods A total of 60 cases with KOA were randomly recruited into a control group and a treatment group,30 cases in each.TCM symptoms,signs and health assessment questionnaire(HAQ)were observed before and after the treatment.Results The tohal therapeutic effect was 90%and 70%in the treatment group and the control group respectively.There was significant difference between the two group(χ~2=48,P=0.003).Body signs and HAQ were also greatly improved in the treatment group(t=0.004、P=0.008).Conclusion Gubiqing can not only restrain chondrocyte apoptosis in knee osteoarthritis combined with synovitis,but also relive the damage of articular cartilage.

Adoptive Transfer ; methods ; trends ; Cells ; pathology ; Cells, Cultured ; pathology ; Humans